LOCUS       NZ_CP024574             9326 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF274 isolate INF274 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP024574
VERSION     NZ_CP024574.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357268
            Assembly: GCF_002753605.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: INF274
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:26:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,692
            CDSs (total)                      :: 5,570
            Genes (coding)                    :: 5,416
            CDSs (with protein)               :: 5,416
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 51 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 15 of 154
            Pseudo Genes (multiple problems)  :: 15 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024574.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9326
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF274"
                     /isolate="INF274"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Australia"
                     /collection_date="2013-01"
     gene            join(9280..9326,1..166)
                     /locus_tag="CTI69_RS29465"
                     /old_locus_tag="CTI69_29275"
     CDS             join(9280..9326,1..166)
                     /locus_tag="CTI69_RS29465"
                     /old_locus_tag="CTI69_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="WP_099730323.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTPDRAEAKKIAAQAA
                     EQGASYKITSAQFNNRVHMTAELTK"
                     /name="DUF1471 domain-containing protein"
     gene            complement(972..1376)
                     /locus_tag="CTI69_RS29475"
                     /old_locus_tag="CTI69_29280"
                     /pseudo=""
     CDS             complement(972..1376)
                     /locus_tag="CTI69_RS29475"
                     /old_locus_tag="CTI69_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1376..1570)
                     /locus_tag="CTI69_RS29480"
                     /old_locus_tag="CTI69_29285"
     CDS             complement(1376..1570)
                     /locus_tag="CTI69_RS29480"
                     /old_locus_tag="CTI69_29285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_099730332.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2303..4003)
                     /locus_tag="CTI69_RS29500"
                     /old_locus_tag="CTI69_29305"
     CDS             complement(2303..4003)
                     /locus_tag="CTI69_RS29500"
                     /old_locus_tag="CTI69_29305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697690.1"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage dCTP deaminase"
                     /protein_id="WP_225368539.1"
                     /translation="MSLARKLREKRSNPIHSANEDSSGGDSINQITSRQSMDLVIGFCG
                     AVGSGIKSIVDVTEEELDDLGYDVYRIRVSELMQKLFSEWRDDIVTPFDRYHKLQDFGD
                     ALRNKYYPHILSEAVIHEIKIEKGIDTSSKKNKKAFLVDQLKHQDEIELLRMVYQHNFY
                     LLGVIRSEVERKRNLRDEGLSKSEVDIVIHHDRKSESDYGQQTAKAILDSDYFVKNNQG
                     QRAHLKAKVKRFIGLIHGLNGLTPTTYEKGMYAAHSASLQSACLSRQVGAAILDVNGNL
                     ISVGRNDVPKFNGGLYSADDGVNDHRCVYKGGKCYNEVRKLKIKSKISQILMSESACEL
                     GVKLTEDQAEQLADVIYKKTPVSSIIEYSRSIHAEMDAITSLARLGNGGFSDKVLYTTT
                     FPCHNCARHIVAVGITKVVYIEPYEKSLALELHDDAITEVNEHGKVIFESFEGVSPRRY
                     QKFFFSTDERKDSFGNAEKYSTKYKNHIDIQFIDSYLDFENRVVDIFRTNTNIDSGNHV
                     GRTDSTINLSSQPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPDSK"
                     /name="anti-phage dCTP deaminase"
     gene            complement(4016..4198)
                     /locus_tag="CTI69_RS29505"
                     /old_locus_tag="CTI69_29310"
     CDS             complement(4016..4198)
                     /locus_tag="CTI69_RS29505"
                     /old_locus_tag="CTI69_29310"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080882243.1"
                     /translation="MGKELSLNKIYDKNSRYINDSRGHELGKEKKVFDFEVEMIKKRRE
                     DAIRKIIEDSKSLKW"
                     /name="hypothetical protein"
     gene            4586..4795
                     /locus_tag="CTI69_RS29510"
                     /old_locus_tag="CTI69_29315"
     CDS             4586..4795
                     /locus_tag="CTI69_RS29510"
                     /old_locus_tag="CTI69_29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            complement(5638..6039)
                     /locus_tag="CTI69_RS29520"
                     /old_locus_tag="CTI69_29320"
     CDS             complement(5638..6039)
                     /locus_tag="CTI69_RS29520"
                     /old_locus_tag="CTI69_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(6039..6230)
                     /locus_tag="CTI69_RS29525"
                     /old_locus_tag="CTI69_29325"
     CDS             complement(6039..6230)
                     /locus_tag="CTI69_RS29525"
                     /old_locus_tag="CTI69_29325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7149..7406)
                     /locus_tag="CTI69_RS29540"
                     /old_locus_tag="CTI69_29340"
     CDS             complement(7149..7406)
                     /locus_tag="CTI69_RS29540"
                     /old_locus_tag="CTI69_29340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_099730337.1"
                     /translation="MKKKSLALELHDDAITEVNEHGKVIFESFEGVSPRRYQKISSFLQ
                     MKGKIVSVTQKSTPLNIKNHIDIQFIDSYLDFENRVVDIF"
                     /name="hypothetical protein"
     gene            complement(7576..7932)
                     /locus_tag="CTI69_RS29785"
     CDS             complement(7576..7932)
                     /locus_tag="CTI69_RS29785"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157771187.1"
                     /translation="MKKSMYAAHSASLQSACLSRQVGAAILDVNGNLISVGRNDVPKFN
                     GGLYSADDGVNDHRCVYKGGKCYNEVRKLKIKSKISQILMSESACELGVKLTEDQAEQL
                     ADVIYKKKHQYLLL"
                     /name="hypothetical protein"
     gene            complement(8426..8581)
                     /locus_tag="CTI69_RS29790"
     CDS             complement(8426..8581)
                     /locus_tag="CTI69_RS29790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157771188.1"
                     /translation="MDLVIGFCGAVGSGIKSIVDVTEEELDDLGYDVYRIRVSELMQKK
                     LFFRMA"
                     /name="hypothetical protein"
     Typing          1692..2099
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.355"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          6352..6760
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.404"
                     /coverage="99.0"
                     /evalue="1.26e-177"
ORIGIN
        1 gtctttcggt gcttttgctc agagcgtcag cgctaccgcc tccaccccgg atcgtgctga
       61 agcaaaaaaa attgccgcac aggccgctga acaaggtgcg tcatacaaaa tcaccagcgc
      121 tcagttcaat aaccgtgtcc acatgacggc tgaactgacc aaataagatg gtcgcaaagg
      181 gggctgccga acaggccgcc gggaggcggc ccgtttcgtc cccttagcag gaaaaccttc
      241 aggccccttc tggggctttt tttttttgca tccagcattt taacataaag gcatgattgt
      301 acgcaccgct aaaacggcct cagggcgttt ctgacggaaa accggtaaaa caggcaacaa
      361 accacccgaa aacccgccag aaacgcgcag gctcttttta cggcatgcat gagtatgcac
      421 gcaagtgcat agcgcgaaaa ttcacgccat tcagccagag taagtcctgt ttccggcgtt
      481 ctttccatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg aagcgttgcc
      541 ggcgcagccg gaaaaccggt tttcagcacg ttcaggaatc gggttcgtcg acggccggca
      601 atcacgttat ggcaagtcgc cggacgactc accacacgat tcaccggcga ctcttcacta
      661 gagtcgtacc cagagtcgcc gcgtctaaaa aaaaacaacc aaacggggag tcgtgtctag
      721 agtcgggtcg ttattaactt atttttttat agtcctgagt cgttaccgac tctatgacga
      781 ctcgccgggg actctatgac gagtcgtcat agagtcggat ctatataaat aatacaaatc
      841 tgcaaaatat taattttgcg tggggtgtgg gtatttttta gtggtgagga gcgttcagag
      901 atctgacgat tttttgggaa ggttcaggaa ctcccgggat ggccggagag gaaaacggaa
      961 gcatgacggg gtcaggattt tttccagaat ttccaccatg gcgccgggga ttcagttaat
     1021 tttgcccggt gattttcgag aaggagcatg gcctgtttca ggctgtcaat gtgcgaatcc
     1081 ttggacaaaa aagtaaggta ttcaggtgtt caatctcctg tttcagatga cgaatcacct
     1141 gttcactctg gtcagcagag gctgtatcac gctgttcaga gacctgtcca gaatggtgtt
     1201 cacccttcgg ttgctcaaag ggaccaaaga cacgtaaaag ctctgaagta tctaccattt
     1261 tttgcattgc gttcaccgct acaggtggac aggtcacctg ctttaatcaa cctgtgcagt
     1321 gttgttctac tcttacccgt cagtcgggca gcctcactaa tagacactct ggccatcagt
     1381 taccatcctg taattcatcc agtctcgagg acagtgtacg gaaaaaaaga tgctccgcat
     1441 catcgtggag tttctcacaa aggtcggcct caacatcgag atcaagctcg ttaagcttct
     1501 ccagcaggag cagagactga ttctggatga atttagccat gttcagcgct gttttctgtt
     1561 gtttcgtcat aagaagcctc agagcgtcag tgctcagtgt aagaacagtc tcttttccgc
     1621 ttcctcgctc actcagtcgc tacgctcctg ccgttcggct gcggcgagcg gcaggagcat
     1681 gcgtgtaaaa gctcgagaaa accgagcggt ctcgtgatgg ggaaaaggaa aggccgcaca
     1741 gcggccgttt ttccataggc tccgcccccc tgacgagcat cacggaatct gacgctcaaa
     1801 tcagtggcgg cgaaacccga caggactata aagataccag gcgtttcccc ctgatggctc
     1861 cctcgtgcgc tctcctgttc ctgccattcg gtttaccggt gtcattccgc tgttatggcc
     1921 gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
     1981 actgtatgca cgaacccccc gttcagtccg actgctgcgt cttatccggt aactatcatc
     2041 ttgagtccaa cccggtaaga catgcaaaag caccactggc agcagccacc ggtaactgat
     2101 ttagaggagt tagtcttgaa gttatgcgcc agttaaggct aaactgaaag aacaagtttt
     2161 ggtgactgcg ctcctctaag ccagttacct tggttcatag agccgcaaac tctagaacct
     2221 taaaaaaacc gccccgcaaa ggcggttttt ttctttctcg tctcaaggtt aagctctgta
     2281 taacatcaga gtcaacagat cttcatttcg agtctggtgg tactggtggt actggtggta
     2341 ctggcggtac tggcggtact ggcggtactg gcggtacggg cggtacgggc ggtacgggcg
     2401 gtacgggcgg tacgggctgg gaagataaat taatagtcga atcagttctc ccgacatgat
     2461 tgccagaatc aatatttgtg ttagttctaa aaatatcaac gactcgattt tcgaaatcta
     2521 aatagctatc aataaactgt atatcaatgt gatttttata tttagtggag tacttttctg
     2581 cgttaccgaa actatctttc ctttcatctg tagaaaagaa gaatttttga tacctacgag
     2641 gtgatacacc ttcaaaggat tcaaatataa ccttcccatg ctcattcacc tcggttatag
     2701 catcatcatg caattctaaa gcgaggcttt tttcatatgg ctctatataa acaacttttg
     2761 ttattccaac agcgacaata tgtctcgcgc agttatgaca aggaaatgtg gttgtatata
     2821 aaactttatc agaaaaacct ccattaccta gtctagctaa tgatgtaata gcatccatct
     2881 ctgcatgaat tgagcgagag tactctataa tagaagatac tggtgttttt ttatatatta
     2941 catcagccag ttgctctgct tgatcttctg ttaatttaac accaagttca caagcgcttt
     3001 ccgacataag tatttgtgaa attttgcttt taatctttag ttttctaact tcattataac
     3061 atttaccacc tttgtataca caacgatgat cattgacacc atcatccgca ctataaagcc
     3121 caccattgaa tttaggtaca tcattacgcc caacggaaat cagattccca ttaacatcca
     3181 atatagcagc accaacttga cgagaaaggc atgcagattg cagagatgct gaatgagctg
     3241 catacatacc tttttcatac gtggttggag ttaaaccatt tagcccatgt atcaagccaa
     3301 taaaacgctt aaccttagcc ttaaggtgcg ccctttgtcc ttgattattc ttaacaaaat
     3361 aatcagagtc aagtatagct ttcgcagtct gctgaccata atcgctctct gattttctat
     3421 cgtgatgaat aactatatcg acttctgact tactcagccc ttcatctcta agattacgct
     3481 ttctttctac ttcacttcta attacaccta gtaaataaaa attatgttgg tagaccatcc
     3541 ttaaaagttc aatttcatcc tgatgcttca actgatcgac caaaaaagcc tttttgtttt
     3601 ttttacttga ggtgtcaatg cctttttcta tttttatttc atggattaca gcttcagata
     3661 gtatatgtgg gtaatattta tttcttaacg catcaccaaa atcttgcagc ttatgatatc
     3721 tatcaaacgg agtaacaata tcatcacgcc attctgaaaa aagtttttgc ataagctcgc
     3781 taactcttat gcgataaaca tcatatccta gatcatctag ttcttcttca gtcacatcaa
     3841 caattgactt tattcctgag ccaacagcac cgcaaaatcc tataacaaga tccatagact
     3901 gtctggaggt tatttggttt atgctatccc ctccagagga gtcctcgtta gcactatgaa
     3961 tcggattact tcttttttct cttaactttc tagccaggct cataaaagac ctcacttacc
     4021 attttaatga ttttgaatcc tcaattatct tccttatagc gtcctctctt ctttttttaa
     4081 tcatttcaac ttcaaaatcg aaaactttct tttcctttcc cagctcatgc cctctagaat
     4141 cattaatata tctagaattc ttatcataaa ttttatttag agatagctct ttacccatca
     4201 caccctcctg caagacgcat ggattttact gttatcttta taacaggcaa tagccaagaa
     4261 acgtaatcga tagagatttt tttttcttat cctaaacatt aggaaattcg gcaagagaaa
     4321 agcaagagag attacgccaa actaaccatt atttttcaat ttgttaaaat aaaatcatca
     4381 atgttaacat ttctatccac cacctcattt ggtacctaaa atccagagat aaacctctgg
     4441 tcttgatgcg ctcaattctt gttctttttt tttgaatgat atccgcaaat tgtttcgatt
     4501 ttaatcaact aatggtcgtc ctatcattta tcacatcagg gcaacaacgc cccatacaga
     4561 acaaaatcac ttaaggaata cgaccatgaa atccatcaaa actttcgttg cagttgccgc
     4621 tctatccatg atgtctttcg gtgcttttgc tcagagcgtc agcgctaccg cctccaccct
     4681 ggatcgtgct gaagcaaaaa ttgccgcaca ggccgctgaa caaggtgcgt catacaaaat
     4741 caccagcgct cagttcaata accgtgtcca catgacggct gaactgacca aataagatgg
     4801 tcgcaaaggg agctgccgaa caggccgccg ggaggcggcc cgtttcgtcc cttagcagga
     4861 aaaccttcag gccccttctg gggctttttt gcatctgtta tttaacataa aggcatgatt
     4921 gtacgcaccg ctaaaacggc ctcagggcgt ttctgacgga aaaccggtaa aacaggcaac
     4981 aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt tttacggcat gcatgagtat
     5041 gcacgcaagt gcatagcgcg aaaattcacg ccattcagcc agagtaagtc ctgtttccgg
     5101 cgttctttcc atccgaaaac ggtgaacggg gacgcaattt attgcgtggc cgtgaagcgt
     5161 tgccggcgca gccggaaaac cggttttcag cacgttcagg aatcgggttc gtcgacggcc
     5221 ggcaatcacg ttatggcaag tcgccggacg actcaccaca cgattcaccg gcgactcttc
     5281 actagagtcg tacccagagt cgccgcgtct aaaaaacaac caaacgggga gtcgtgtcta
     5341 gagtcgggtc gttattaact tattgttttt atagtcctga gtcgttaccg actctatgac
     5401 gactcgccgg ggactctatg acgagtcgtc atagagtcgg atctatataa ataaaattta
     5461 aaaaacgaaa tttcaggaaa gtaacccact gatattaaat acaaatctgc aaaatattaa
     5521 ttttgcgtgg ggtgtgggta tttttagtgg tgaggagcgt tcagagatct gacgattttt
     5581 gggaaggttc aggaactccc cgggatggcc ggagaggaaa acggaagcat gacggggtca
     5641 ggattttttc cagaatttcc accatggcgc cggggattca gttaattttg cctggtgatt
     5701 ttcgagaagg agcatggcct gtttcaggct gtcaatgtgc gaatccttgg acaaaagtaa
     5761 ggtattcagg tgttcaatct cctgtttcag atgacgaatc acctgttcac tctggtcagc
     5821 agaggctgta tcacgctgtt cagagacctg tccagaatgg tgttcaccct tcggttgctc
     5881 aaagggacca aagacacgta aaagctctga agtatctacc atttttgcat tgcgttcacc
     5941 gctacaggtg gacaggtcac ctgctttaat caacctgtgc agtgttgttc tactcttacc
     6001 cgtcagtcgg gcagcctcac taatagacac tctggccatc agttaccatc ctgtaattca
     6061 tccagtctcg aggacagtgt acggaaaaga tgctccgcat catcgtggag tttctcacaa
     6121 aggtcggcct caacatcgag atcaagctcg ttaagcttct ccagcaggag cagagactga
     6181 ttctggatga atttagccat gttcagcgct gttttctgtt gtttcgtcat aagaagcctc
     6241 agagcgtcag tgctcagtgt aagaacagtc tcttttccgc ttcctcgctc actcagtcgc
     6301 tacgctcctg ccgttcggct gcggcgagcg gcaggagcat gcgtgtaaaa gctcgagaaa
     6361 accgagcggt ctcgtgatgg ggaaaaggaa aggccgcaca gcggccgttt tttccatagg
     6421 ctccgccccc cctgacgagc atcacggaat ctgacgctca aatcagtggc ggcgaaaccc
     6481 gacaggacta taaagatacc gggcgtttcc cctgatggct ccctcgtgcg ctctcctgtt
     6541 cctgccattc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
     6601 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     6661 cgttcagtcc gactgctgcg tcttatccgg taactatcat cttgagtcca acccggtaag
     6721 acatgcaaaa gcaccactgg cagcagccac cggtaactga tttagaggag ttagtcttga
     6781 agttatgcgc cagttaaggc taaactgaaa gaacaagttt tggtgactgc gctcctctaa
     6841 gccagttacc ttggttcata gagccgcaaa ctctagaacc ttaaaaaaaa ccgccccgca
     6901 aaggcggttt ttttttttct cgtctcaagg ttaagctctg tataacatca gagtcaacag
     6961 atcttcattt cgagtctggt ggtactggtg gtactggtgg tactggcggt actggcggta
     7021 ctggcggtac tggcggtacg ggcggtacgg gcggtacggg cggtacgggc ggtacgggct
     7081 gggaagataa attaatagtc gaatcagttc tcccgacatg attgccagaa tcaatatttg
     7141 tgttagttct aaaaaatatc aacgactcga ttttcgaaat ctaaatagct atcaataaac
     7201 tgtatatcaa tgtgattttt tatatttagt ggagtacttt tctgcgttac cgaaactatc
     7261 tttcctttca tctgtagaaa agaagaaatt ttttgatacc tacgaggtga tacaccttca
     7321 aaggattcaa atataacctt cccatgctca ttcacctcgg ttatagcatc atcatgcaat
     7381 tctaaagcga ggcttttttt tttcatatgg ctctatataa acaacttttg ttattccaac
     7441 agcgacaaat atgtctcgcg cagttatgac aaggaaatgt ggttgtatat aaaactttat
     7501 cagaaaaacc tccattacct agtctagcta atgatgtaat agcatccatc tctgcatgaa
     7561 ttgagcgaga gtactctata atagaagata ctggtgtttt tttttatata ttacatcagc
     7621 cagttgctct gcttgatctt ctgttaattt aacaccaagt tcacaagcgc tttccgacat
     7681 aagtatttgt gaaattttgc ttttaatctt tagttttcta acttcattat aacatttacc
     7741 acctttgtat acacaacgat gatcattgac accatcatcc gcactataaa gcccaccatt
     7801 gaatttaggt acatcattac gcccaacgga aatcagattc ccattaacat ccaatatagc
     7861 agcaccaact tgacgagaaa ggcatgcaga ttgcagagat gctgaatgag ctgcatacat
     7921 actttttttc atacgtggtt ggagttaaac catttagccc atgtatcaag ccaataaaac
     7981 gcttaacctt agccttaagg tgcgcccttt gtccttgatt attcttaaca aaataatcag
     8041 agtcaagtat agctttcgca gtctgctgac cataatcgct ctctgatttt ctatcgtgat
     8101 gaataactat atcgacttct gacttactca gcccttcatc tctaagatta cgctttcttt
     8161 ctacttcact tctaattaca cctagtaaat aaaaaattat gttggtagac catccttaaa
     8221 agttcaattt catcctgatg cttcaactga tcgaccaaaa aaagcctttt tttttttttt
     8281 tttttacttg aggtgtcaat gccttttttt cttttttatt tcatggatta cagcttcaga
     8341 tagtatatgt gggtaatatt tatttcttaa cgcatcacca aaatcttgca gcttatgata
     8401 tctatcaaac ggagtaacaa tatcatcacg ccattctgaa aaaaagtttt ttttgcataa
     8461 gctcgctaac tcttatgcga taaacatcat atcctagatc atctagttct tcttcagtca
     8521 catcaacaat tgactttatt cctgagccaa cagcaccgca aaatcctata acaagatcca
     8581 tagactgtct ggaggttatt tggtttatgc tatcccctcc agaggagtcc tcgttagcac
     8641 tatgaatcgg attacttctt tttttctctt aactttctag ccgggctcat aaaagacctc
     8701 acttaccatt ttaatgattt tgaatcctca attatcttcc ttatagcgtc ctctcttctt
     8761 tttttttaat catttcaact tcaaaatcga aaactttttc ttttcctttc ccagctcatg
     8821 ccctctagaa tcattaatat atctagaatt cttatcataa attttattta gagatagctc
     8881 tttacccatc acaccctcct gcaagacgca tggattttac tgttatcttt ataacaggca
     8941 atagccaaga aacgtaatcg atagagattt tttttcttat cctaaacatt aggaaattcg
     9001 gcaagagaaa agcaagagag attacgccaa actaaccatt atttttttca atttgttaaa
     9061 ataaaatcat caatgttaac atttctatcc accacctcat ttggtaccta aaatccagag
     9121 ataaacctct ggtcttgatg cgctcaattc ttgttctttt tttttttgaa tgatatccgc
     9181 aaattgtttc gattttaatc aactaatggt cgtcctatca tttatcacat cagaacaaca
     9241 acgccccata cagaacaaaa tcacttaagg aatacgacca tgaaatccat caaaactttc
     9301 gttgcggttg ccgctctatc catgat
//
