LOCUS       NZ_CP024568             4510 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF278 isolate INF278 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP024568
VERSION     NZ_CP024568.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357272
            Assembly: GCF_002752975.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JAN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: INF278
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 115x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:26:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,691
            CDSs (total)                      :: 5,569
            Genes (coding)                    :: 5,415
            CDSs (with protein)               :: 5,415
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 50 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 16 of 154
            Pseudo Genes (multiple problems)  :: 15 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024568.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF278"
                     /isolate="INF278"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Australia"
                     /collection_date="2014-01"
     gene            628..1074
                     /locus_tag="CTI68_RS29515"
                     /old_locus_tag="CTI68_29310"
     CDS             628..1074
                     /locus_tag="CTI68_RS29515"
                     /old_locus_tag="CTI68_29310"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(1616..1858)
                     /locus_tag="CTI68_RS29520"
                     /old_locus_tag="CTI68_29320"
     CDS             complement(1616..1858)
                     /locus_tag="CTI68_RS29520"
                     /old_locus_tag="CTI68_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     gene            2041..2361
                     /locus_tag="CTI68_RS29525"
                     /old_locus_tag="CTI68_29325"
     CDS             2041..2361
                     /locus_tag="CTI68_RS29525"
                     /old_locus_tag="CTI68_29325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2565..2921
                     /locus_tag="CTI68_RS29530"
                     /old_locus_tag="CTI68_29330"
     CDS             2565..2921
                     /locus_tag="CTI68_RS29530"
                     /old_locus_tag="CTI68_29330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     gene            3750..3941
                     /locus_tag="CTI68_RS29545"
                     /old_locus_tag="CTI68_29345"
     CDS             3750..3941
                     /locus_tag="CTI68_RS29545"
                     /old_locus_tag="CTI68_29345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3941..4351
                     /locus_tag="CTI68_RS29550"
                     /old_locus_tag="CTI68_29350"
     CDS             3941..4351
                     /locus_tag="CTI68_RS29550"
                     /old_locus_tag="CTI68_29350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(3222..3631)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttattacat tacgcagtat tactttctgt aaggtgaatc caagtcgtcg gcgattcgtg
       61 tacgacttgc cgacgactcg tcatagagtc atcgacgact cacttaagac tatcagtaac
      121 ttatgacgac ttgactctag agtcgaatcg tcattttggt gtttttttga ccttggcgac
      181 tctagagtca agtcacgagt cgaatcgccg gtgaatcgtg tggtgagtcg tccggcgact
      241 tgcataacgt gattgccggc cgtcgacgaa cccgattcct gaacgtgctg aaaaccgttt
      301 ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc gttcaccgtt
      361 ttcagtctgg tttggtgcgg gttttgagtt gtctggcctg aattcaggct gaaatccgcc
      421 accagtcagt ctggtgcata acaattcatg cccgtaaaac gccctggagc gattttgagg
      481 cgttttcagg gggtaagacg taggatttaa tggattacgc cccagaatcg tttctagagc
      541 gtttgagcgg tgcgcgtaat gaggcgttat ggtaaataat tgctcgtgaa aacgttatta
      601 acatttttat cttatgaggt gatgtgagtg aacgataaat caggctttag ttttatgaag
      661 atcggtgcag gtgcaaaagt gactggttta agaatgaaaa ggaatgtgat ggttgttgat
      721 gatctgtctc acgtagagag ctttgttact gttggtgagc gaggcgaagt tattaatctc
      781 gatgcggatg ggaacgaact acacacacca gacagttatc gtgaaaagtt aaaaatagaa
      841 cgcggtgaac tccaggcttc cttggagaaa gccaaggagg aaatcaactc aatagataac
      901 gatataactt cgcaaaagat taagaaccag ttgagtgctg cactatctca accaatggat
      961 gcagaaggtg agtggaacac taagagaatc ctacaagggg ttttagattt atctaaacaa
     1021 gtgggctgtg gtgtactcac ggctgcgatc acgcattatc ttggagttag ttaataaact
     1081 acctatgccc ccatggtggg tatacaaaaa ccatggggag cggagcgacc ttcgtctagc
     1141 tcttttcttg tgtgtctcat cttgcaacgg attcgatttg tgtggggtta gtgtcagact
     1201 tcttggggtc agtttggata tagagaatta ttgtacggta agcctgtgtt ttgaacaggc
     1261 ttacctctcg caatgatgta ctcccgcgct atacgccgtt tcccgtgaat tttccttaat
     1321 cggataacct aaatgcgata cagaaagagc ggtgacttaa aagcgatcgt agctgaggta
     1381 aaatcaatcc cttacgcatc aatattggtt aaataatgcg cttaacgtac aaaagtttcc
     1441 gatatccaaa ctgaccccta tttggcggct tttcttattt tcttgatttt cccgcatctg
     1501 aacgtttctc tcggactctt ccattattat tacgatctcg tggctgagtt ccgcctcctg
     1561 gtttagggac gacgacatgc gggttaagtg gtctacggtc tgccattttc atacctcact
     1621 ctgcataaac aaatagttgc cagcggatgt ccccctgtcc gggactatgt tgatgatgat
     1681 tttgtggtgg aagtgttttc cctcctgcga tagttacttc tttaccgcaa cctgtgcatt
     1741 tgtaaattcc ttcatacgtg cacgtatcgc caggcttgtg ttgtgtatca aaaactgggt
     1801 taaatttcgg atctgcttca aaaatcttgg ttttatttcc tgagcgaaca agcgccatat
     1861 gacctccaac tattcttttt ttgcacagta gcactgtgct tttgtgcatt ttatatcatt
     1921 atggtttttt gcacaagtgc acagtgctat tgtgcatttc tggcgtttgt tgtactatgg
     1981 tggtggtaaa aggtgatcta gaacttgcca agttgcggca acatttgagg taaaagaaaa
     2041 atgcagcaaa atcatgatag tgatgatgat tgccgcattc agttccgaga cgattggcta
     2101 taccagtttt ataaatacgg taaagccaat cgaaaaattc cagctacgat tagtgtttca
     2161 ttaggtcgca agctggatat gctgaatgct gctgataaaa ttggagattt acggtctccc
     2221 ccggggaacc gatttgaaaa tctcaaccct ccattgacag ggtattcatc tataagggtg
     2281 aatgataagt accgcttgat atttaagtgg ggtgagaacg gagtcgagga tttgtatctc
     2341 gatccacatg attaccgata ggtaacagac cggaactgtt atcttacaaa acaagtaggt
     2401 gaggcatttc ctcacctacg ttaaaacgtc atcacaaata atgattaata aagtaacgcg
     2461 acgtaactat atttaagata attactaatc gatacagctg gtaacagaag aaatacttat
     2521 cttcctcatc taacagagag aagcgaccaa ttcaggagta aagcatgtcc caagcaacca
     2581 gaaaaccatc gactgtagga gatattattc tttatgaata tctcgagcca agtggtttaa
     2641 aaatcaatga cttggctgaa atgttaaacg tgcatagaaa ttctgcaagt gctttggtta
     2701 acaataatag gaaactgtct accgatatgg cgtttcggtt agctaaagca ttcgatacta
     2761 ccgctaagtt ctggttaaat atacagcagt ctgttgattt gtgggaggtt acgagcagcc
     2821 cacgccttca ggaagaaata gaaagagtca ctcccgctca tgaaattata gaaaggaagc
     2881 acaatgctga atctttggaa aaaaaacatg cggtcgcatg atttttttaa aaaaatttaa
     2941 ccaaactaga agaaggcctc tgtaatagtg tgccttcttt ttttgtaacc gctaaaggtc
     3001 atcatcattt tcttttacga gcacttgaga tccggtttcg gtacgcgtaa tctcttgctc
     3061 tgtaaacgga aaaaccgcct tgcagggcgg tttttccgaa ggttcaaaca cttgcaggtg
     3121 attgaaccaa ggtaactggc ttggaggagc gcagtcacca aaacttgttc tttcagttta
     3181 gccttaacag gcgcatgact tcaagactaa ctcctctaaa tcagttacca gtggctgctg
     3241 ccagtggcgc ttttgcatgt cttaccgggt tggactcaag acgatagtta ccggataaga
     3301 cgcagcagtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
     3361 tcccggaact gagtgtctgg cgtggaatga gataaacgcg gccataacag cggaatgaca
     3421 ccggtaaacc gaatggcagg aacaggagag cgcacgaggg agccatcagg gggaaacgcc
     3481 tgatatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca atttctatga
     3541 tgcttgtcag gggggcggag cctatggaaa aacggccgct aagcggcctt tccttctgcc
     3601 catcacgcgt ccgctcggtt ttctcgagca tttatacgca tagtcctacc gctcgccgca
     3661 gccgaacggc aggagcgtag cgactgagtg agcgaggaag cggaaaagag actggtttta
     3721 cactgagcac tgacgctctg aggcttctta tgacgaaaca acagaaaaca gcgctgaaca
     3781 tggctaaatt catccagaat cagtctctgc tcctgctgga gaagcttaac gagcttgatc
     3841 tcgatgttga ggccgacctt tgtgagaaac tccacgatga tgcggagcat cttttccgta
     3901 cactgtcctc gagactggat gaattacagg atggtaactg atggccagag tgtctattag
     3961 tgaagcggca cggcttgtta aggttagtcg ccccacaatt tacaagatga ttaacagtgg
     4021 gaagttaagt tacacctctg ttgtaaagca cgggaagatc attaaagtta ttgatacttc
     4081 agagcttatt agagtttttg gttctcttga tggtgtcatc gatactgtaa aaagtgacgc
     4141 aggatctaca ggtgttaaca gcgttggttt acatgattta caacatagaa ttgctttact
     4201 agaagctgaa aacgatggtc ttaaaggggc tgttaaggct cgagatgagc atattgattc
     4261 ccttcgtcag gctatgcagc ttctggagca caagcacgaa ccttcttcac cacccgattc
     4321 cccgtggtgg aaattctgga aaaaatcctg actccgtcat gcttccgttt tcctctccgg
     4381 ccatcccggg gagttcctga accttcccaa aaatcgccag atctctgaac gctcctcacc
     4441 actaaaaata cccacacccc acgcaaaatt aaaattttga agataattca ttaatatcag
     4501 ttagttgtgg
//
