LOCUS       NZ_CP024561             4510 bp    DNA     circular CON 26-DEC-2023
DEFINITION  Klebsiella pneumoniae strain INF164 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP024561
VERSION     NZ_CP024561.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07211280
            Assembly: GCF_002753555.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: INF164
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/26/2023 04:24:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,609
            Genes (coding)                    :: 5,445
            CDSs (with protein)               :: 5,445
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 55 of 164
            Pseudo Genes (incomplete)         :: 109 of 164
            Pseudo Genes (internal stop)      :: 16 of 164
            Pseudo Genes (multiple problems)  :: 16 of 164
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024561.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF164"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
     gene            join(4488..4510,1..388)
                     /locus_tag="CTI75_RS29715"
                     /old_locus_tag="CTI75_29515"
     CDS             join(4488..4510,1..388)
                     /locus_tag="CTI75_RS29715"
                     /old_locus_tag="CTI75_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            1175..1621
                     /locus_tag="CTI75_RS29720"
                     /old_locus_tag="CTI75_29520"
     CDS             1175..1621
                     /locus_tag="CTI75_RS29720"
                     /old_locus_tag="CTI75_29520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(2163..2405)
                     /locus_tag="CTI75_RS29725"
                     /old_locus_tag="CTI75_29530"
     CDS             complement(2163..2405)
                     /locus_tag="CTI75_RS29725"
                     /old_locus_tag="CTI75_29530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     gene            2588..2908
                     /locus_tag="CTI75_RS29730"
                     /old_locus_tag="CTI75_29535"
     CDS             2588..2908
                     /locus_tag="CTI75_RS29730"
                     /old_locus_tag="CTI75_29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3112..3468
                     /locus_tag="CTI75_RS29735"
                     /old_locus_tag="CTI75_29540"
     CDS             3112..3468
                     /locus_tag="CTI75_RS29735"
                     /old_locus_tag="CTI75_29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     gene            4297..4488
                     /locus_tag="CTI75_RS29750"
                     /old_locus_tag="CTI75_29555"
     CDS             4297..4488
                     /locus_tag="CTI75_RS29750"
                     /old_locus_tag="CTI75_29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3769..4178)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agcggcacgg cttgttaagg ttagtcgccc cacaatttac aagatgatta acagtgggaa
       61 gttaagttac acctctgttg taaagcacgg gaagatcatt aaagttattg atacttcaga
      121 gcttattaga gtttttggtt ctcttgatgg tgtcatcgat actgtaaaaa gtgacgcagg
      181 atctacaggt gttaacagcg ttggtttaca tgatttacaa catagaattg ctttactaga
      241 agctgaaaac gatggtctta aaggggctgt taaggctcga gatgagcata ttgattccct
      301 tcgtcaggct atgcagcttc tggagcacaa gcacgaacct tcttcaccac ccgattcccc
      361 gtggtggaaa ttctggaaaa aatcctgact ccgtcatgct tccgttttcc tctccggcca
      421 tcccggggag ttcctgaacc ttcccaaaaa tcgccagatc tctgaacgct cctcaccact
      481 aaaaataccc acaccccacg caaaattaaa attttgaaga taattcatta atatcagtta
      541 gttgtgggtt attacattac gcagtattac tttctgtaag gtgaatccaa gtcgtcggcg
      601 attcgtgtac gacttgccga cgactcgtca tagagtcatc gacgactcac ttaagactat
      661 cagtaactta tgacgacttg actctagagt cgaatcgtca ttttggtgtt tttttgacct
      721 tggcgactct agagtcaagt cacgagtcga atcgccggtg aatcgtgtgg tgagtcgtcc
      781 ggcgacttgc ataacgtgat tgccggccgt cgacgaaccc gattcctgaa cgtgctgaaa
      841 accgtttttc cggctgcgcc ggcaacgctt cacggccacg caataaattg cgtccccgtt
      901 caccgttttc agtctggttt ggtgcgggtt ttgagttgtc tggcctgaat tcaggctgaa
      961 atccgccacc agtcagtctg gtgcataaca attcatgccc gtaaaacgcc ctggagcgat
     1021 tttgaggcgt tttcaggggg taagacgtag gatttaatgg attacgcccc agaatcgttt
     1081 ctagagcgtt tgagcggtgc gcgtaatgag gcgttatggt aaataattgc tcgtgaaaac
     1141 gttattaaca tttttatctt atgaggtgat gtgagtgaac gataaatcag gctttagttt
     1201 tatgaagatc ggtgcaggtg caaaagtgac tggtttaaga atgaaaagga atgtgatggt
     1261 tgttgatgat ctgtctcacg tagagagctt tgttactgtt ggtgagcgag gcgaagttat
     1321 taatctcgat gcggatggga acgaactaca cacaccagac agttatcgtg aaaagttaaa
     1381 aatagaacgc ggtgaactcc aggcttcctt ggagaaagcc aaggaggaaa tcaactcaat
     1441 agataacgat ataacttcgc aaaagattaa gaaccagttg agtgctgcac tatctcaacc
     1501 aatggatgca gaaggtgagt ggaacactaa gagaatccta caaggggttt tagatttatc
     1561 taaacaagtg ggctgtggtg tactcacggc tgcgatcacg cattatcttg gagttagtta
     1621 ataaactacc tatgccccca tggtgggtat acaaaaacca tggggagcgg agcgaccttc
     1681 gtctagctct tttcttgtgt gtctcatctt gcaacggatt cgatttgtgt ggggttagtg
     1741 tcagacttct tggggtcagt ttggatatag agaattattg tacggtaagc ctgtgttttg
     1801 aacaggctta cctctcgcaa tgatgtactc ccgcgctata cgccgtttcc cgtgaatttt
     1861 ccttaatcgg ataacctaaa tgcgatacag aaagagcggt gacttaaaag cgatcgtagc
     1921 tgaggtaaaa tcaatccctt acgcatcaat attggttaaa taatgcgctt aacgtacaaa
     1981 agtttccgat atccaaactg acccctattt ggcggctttt cttattttct tgattttccc
     2041 gcatctgaac gtttctctcg gactcttcca ttattattac gatctcgtgg ctgagttccg
     2101 cctcctggtt tagggacgac gacatgcggg ttaagtggtc tacggtctgc cattttcata
     2161 cctcactctg cataaacaaa tagttgccag cggatgtccc cctgtccggg actatgttga
     2221 tgatgatttt gtggtggaag tgttttccct cctgcgatag ttacttcttt accgcaacct
     2281 gtgcatttgt aaattccttc atacgtgcac gtatcgccag gcttgtgttg tgtatcaaaa
     2341 actgggttaa atttcggatc tgcttcaaaa atcttggttt tatttcctga gcgaacaagc
     2401 gccatatgac ctccaactat tctttttttg cacagtagca ctgtgctttt gtgcatttta
     2461 tatcattatg gttttttgca caagtgcaca gtgctattgt gcatttctgg cgtttgttgt
     2521 actatggtgg tggtaaaagg tgatctagaa cttgccaagt tgcggcaaca tttgaggtaa
     2581 aagaaaaatg cagcaaaatc atgatagtga tgatgattgc cgcattcagt tccgagacga
     2641 ttggctatac cagttttata aatacggtaa agccaatcga aaaattccag ctacgattag
     2701 tgtttcatta ggtcgcaagc tggatatgct gaatgctgct gataaaattg gagatttacg
     2761 gtctcccccg gggaaccgat ttgaaaatct caaccctcca ttgacagggt attcatctat
     2821 aagggtgaat gataagtacc gcttgatatt taagtggggt gagaacggag tcgaggattt
     2881 gtatctcgat ccacatgatt accgataggt aacagaccgg aactgttatc ttacaaaaca
     2941 agtaggtgag gcatttcctc acctacgtta aaacgtcatc acaaataatg attaataaag
     3001 taacgcgacg taactatatt taagataatt actaatcgat acagctggta acagaagaaa
     3061 tacttatctt cctcatctaa cagagagaag cgaccaattc aggagtaaag catgtcccaa
     3121 gcaaccagaa aaccatcgac tgtaggagat attattcttt atgaatatct cgagccaagt
     3181 ggtttaaaaa tcaatgactt ggctgaaatg ttaaacgtgc atagaaattc tgcaagtgct
     3241 ttggttaaca ataataggaa actgtctacc gatatggcgt ttcggttagc taaagcattc
     3301 gatactaccg ctaagttctg gttaaatata cagcagtctg ttgatttgtg ggaggttacg
     3361 agcagcccac gccttcagga agaaatagaa agagtcactc ccgctcatga aattatagaa
     3421 aggaagcaca atgctgaatc tttggaaaaa aaacatgcgg tcgcatgatt tttttaaaaa
     3481 aatttaacca aactagaaga aggcctctgt aatagtgtgc cttctttttt tgtaaccgct
     3541 aaaggtcatc atcattttct tttacgagca cttgagatcc ggtttcggta cgcgtaatct
     3601 cttgctctgt aaacggaaaa accgccttgc agggcggttt ttccgaaggt tcaaacactt
     3661 gcaggtgatt gaaccaaggt aactggcttg gaggagcgca gtcaccaaaa cttgttcttt
     3721 cagtttagcc ttaacaggcg catgacttca agactaactc ctctaaatca gttaccagtg
     3781 gctgctgcca gtggcgcttt tgcatgtctt accgggttgg actcaagacg atagttaccg
     3841 gataagacgc agcagtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     3901 actgccttcc cggaactgag tgtctggcgt ggaatgagat aaacgcggcc ataacagcgg
     3961 aatgacaccg gtaaaccgaa tggcaggaac aggagagcgc acgagggagc catcaggggg
     4021 aaacgcctga tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcaatt
     4081 tctatgatgc ttgtcagggg ggcggagcct atggaaaaac ggccgctaag cggcctttcc
     4141 ttctgcccat cacgcgtccg ctcggttttc tcgagcattt atacgcatag tcctaccgct
     4201 cgccgcagcc gaacggcagg agcgtagcga ctgagtgagc gaggaagcgg aaaagagact
     4261 ggttttacac tgagcactga cgctctgagg cttcttatga cgaaacaaca gaaaacagcg
     4321 ctgaacatgg ctaaattcat ccagaatcag tctctgctcc tgctggagaa gcttaacgag
     4381 cttgatctcg atgttgaggc cgacctttgt gagaaactcc acgatgatgc ggagcatctt
     4441 ttccgtacac tgtcctcgag actggatgaa ttacaggatg gtaactgatg gccagagtgt
     4501 ctattagtga
//
