LOCUS       NZ_CP024541             3825 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KSB1_9D plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP024541
VERSION     NZ_CP024541.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07211282
            Assembly: GCF_002753165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB1_9D
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 02:58:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,668
            CDSs (total)                      :: 5,545
            Genes (coding)                    :: 5,387
            CDSs (with protein)               :: 5,387
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 53 of 158
            Pseudo Genes (incomplete)         :: 105 of 158
            Pseudo Genes (internal stop)      :: 15 of 158
            Pseudo Genes (multiple problems)  :: 15 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_9D"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
     gene            join(3722..3825,1..88)
                     /locus_tag="CTI62_RS29405"
                     /old_locus_tag="CTI62_29200"
     CDS             join(3722..3825,1..88)
                     /locus_tag="CTI62_RS29405"
                     /old_locus_tag="CTI62_29200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            88..489
                     /locus_tag="CTI62_RS29410"
                     /old_locus_tag="CTI62_29205"
     CDS             88..489
                     /locus_tag="CTI62_RS29410"
                     /old_locus_tag="CTI62_29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            1294..1566
                     /locus_tag="CTI62_RS29420"
                     /old_locus_tag="CTI62_29215"
     CDS             1294..1566
                     /locus_tag="CTI62_RS29420"
                     /old_locus_tag="CTI62_29215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019405140.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     Typing          2886..3100
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.562"
                     /coverage="100.0"
                     /evalue="1.62e-49"
     Typing          complement(2389..2905)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.847"
                     /coverage="87.0"
                     /evalue="5.5e-153"
ORIGIN
        1 ggacctttgt gagaaactcc acgatgatgc ggagcatctt ttccgtacac tgtcctcgag
       61 actggatgca ttacaggatg gtaactgatg gccagagtgt ctattagtga ggctgcccga
      121 ctgacgggta agagtagaac aacactgcac aggttaatta aagcaggtga cctgtccacc
      181 tgtagcggtg aacgcaatgc aaaaatggta gatacttcag agcttttacg tgtctttggt
      241 ccctttgaac aaccgaaggg tgaacaccat tctggacagg tctctggaca gcgtgataca
      301 gcctctgctg accagagtga acaggtgatt cgtcatctga aacaggagat tgaacacctg
      361 aacaccttac ttttgtccaa ggattcgcac attgacagcc tgaaacaggc catgctcctt
      421 cttgaaaatc accaggcaaa attaactgaa tccccggcgc catggtggaa attctggaaa
      481 aaatcctgat cccgccatgc ttccattttg ctctccggcc attccgggga gttcctgaac
      541 cttcccaaaa atcgtcagat ccctgaacgc tcctcaccac taaaaatacc cacacactac
      601 gcaaaattaa aattttgcag atagaccttt attttcaatc agttatctgt tttaaattac
      661 gcaattttat tttttgtaag gtgaatccga gtcgtggacg actctatggc gaatcgccga
      721 tgacttgccc acgattcata tgtgaatcac gccatataat cagtacgtta tgatgacttg
      781 actctagagt cgacttgccg ggttggtggc tttttgacca tggcgactct agagtcaagt
      841 cacgagtcga atcgccggtg aattgtctgg tgagtcgtcc ggcgacttgc cataacgtga
      901 ttgccggccg tcgacgaacc cgattcctga tcgtgctgaa aaccggtttt ccggctgcgc
      961 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggataggaa
     1021 gaacgccgga aacaggactt accctggctg aatggcgtga atttccgcgc tatgcacttg
     1081 cgtgcatact catgcatgcc gtaaaaacag agccagcgcg tttctggcgg attttcgggt
     1141 ggtttgttgc ctgttttacc ggtttcccgt caggaacgcc ctgaggccgt tttcgcggtg
     1201 cgcgtaatgg gacgttatgg taaatcaacg gcggatgcct gagtctgtgt tagagtcagc
     1261 ggattgtgat aatcagtgac tcagggagat gttttggcta ctgacgctca ggtaaaagaa
     1321 attaatgcac tcatcaaaaa atacccggac tcatgctcca tctgtcatga agtctatgat
     1381 gaggatgatg tgacctacac cgtttttggg tatgaccgga aaggtaaaat acaggtcacc
     1441 acgggatgct gtgccggaat gctgacagag ccggttctgc tgggtgtctg tgggtgcttt
     1501 gacccggaag aacgtgacga gatcatgcag aatcacccca tggcaaagca gtttttcaca
     1561 gagtgattcc gtatctgttg agtctgaagc ccgccttgtg cgggcttttt catatctgcg
     1621 tgaaatgaga cgttatggta aatctctaaa ggcaatattt cccgtccctt ggagcgaact
     1681 actctgtatt tgatctttgg tgttgatacc ccgctgcttg cagagggggg attcattatc
     1741 gttgttgttt gctgtaattg tctaaaatat atttggggat agatacgggt acacattcag
     1801 cagatattat gtttttcccg gatatattat gataaatgtc tttgtttgca atgattttat
     1861 ctttgaattc aatgcattct tcattcagaa tgaaggtgtt aacaacattg tctttcattc
     1921 ccaaatttgc taattctgaa gaacaacttg caatattatt cgtgttaaaa caagttgtta
     1981 gtattattgc gtatatgtat ggcattaaat aaacctattt aaaaaagtac ggaatgatag
     2041 tgatcttgtt cggaaatata ctagtcagac tttaagtctc agggatatga acctagaaca
     2101 tcgttgtatt aacttgtttg agtgttccaa tcctgaatat tgatgttatt tgagtataaa
     2161 ggaaacataa taaatatcac aaggatattg gttaactatt tatttttaaa taatttcttg
     2221 agatctgacg ttggacgtcg tagtctcttg ccatgtaaac gaaaaaacca ccttgcaggg
     2281 tggtttttcg aaggttcagt aaaccggcaa gtttctgaac cgtggtaaca gtcttgtgcg
     2341 agacgtcacc aaatctgtcc ttttagtgta gcctcagtct ggccaccact tcaagaactc
     2401 ttcgtaaatc tctcgcacat cctgtttacc agtggccgct gccagtggcg ttaagtcgtg
     2461 tctttccggg ttggactcaa gacgatagtt accggaaaag gcgcagcggt cgggctgaac
     2521 ggggggttct tgcatacagc ccagcttgga gcgaactgtc taaacggaac ggggcgtggt
     2581 gatttaggta aagcttccac cactacacgg atgccgcagg acgggaacag gagagcgcaa
     2641 gagggagcca ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca
     2701 ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg
     2761 ctttgccgcg gccttatcgt atccctgtta agtttcttcc tggcatccac caggaaataa
     2821 atttccgccc cattagtaag cctgcaccgc tcgccgcagc cgaacgaccg agcgcagcga
     2881 gtcagtgagc gaggaagcgg aatatcacct gaaccagcat cttctcctga cgccggaaag
     2941 cctcatttca ctcctgccac atgaagcact ttggcaaatc accacttcgg tttaaaatat
     3001 ttagccagta tgcactccgc tagcgctacg tgactggttc agggctgcgc cccgaaaccc
     3061 gctaacaccc ctcagctact ggatatcaca ccatgccatc tgagtatgat gaacaaccgg
     3121 atgcgactga tgctctgttt cccggcgaat atgacctttt tgacttaatg gctcatgtta
     3181 ctgaagagaa cctgcacgcc ccggtggatt ctggcccgcc tgtcggccgg gaagcgttat
     3241 agctttttcg ctggccagcg tacatgtaat acgctggtat tacatgttat ttgtttattt
     3301 tcatgttgtt acagatgatt gtaatacgtt gcgtattacg ctaatacacg aaataactcc
     3361 atttttttca tttaattcat gcagttgcga taaaaatcgc ggctgcgtta ggtgtatgac
     3421 catttaaggg gcaaaaaatt atcattctgg ctgggtgatc tgcgcaggtt aaggatcatc
     3481 ggcgacgaaa aagcgtcttt aactgcttcg ttcatgcttg catgaacgga ttatttttcc
     3541 agactacagg taaactacag gaagactaca aaagttgatg atttaatcct gcgttttagt
     3601 gcgatgacta cagataaact acaaaataac tacaggataa cctcagatcc gttctctgaa
     3661 ccattcgtac atccgacccc cttaccggtt tacactgagc actgatgctc tgagggccct
     3721 tatgacgaaa caacagaaaa cagcgctgaa catggctaaa ttcatccaga atcagtctct
     3781 gctcctgctg gagaagctta acgagcttga tctcgatgtt gaggc
//
