LOCUS       NZ_CP024540             4510 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KSB1_9D plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP024540
VERSION     NZ_CP024540.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07211282
            Assembly: GCF_002753165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB1_9D
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 02:58:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,668
            CDSs (total)                      :: 5,545
            Genes (coding)                    :: 5,387
            CDSs (with protein)               :: 5,387
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 53 of 158
            Pseudo Genes (incomplete)         :: 105 of 158
            Pseudo Genes (internal stop)      :: 15 of 158
            Pseudo Genes (multiple problems)  :: 15 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024540.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_9D"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
     gene            33..224
                     /locus_tag="CTI62_RS29365"
                     /old_locus_tag="CTI62_29155"
     CDS             33..224
                     /locus_tag="CTI62_RS29365"
                     /old_locus_tag="CTI62_29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            224..634
                     /locus_tag="CTI62_RS29370"
                     /old_locus_tag="CTI62_29160"
     CDS             224..634
                     /locus_tag="CTI62_RS29370"
                     /old_locus_tag="CTI62_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            1421..1867
                     /locus_tag="CTI62_RS29375"
                     /old_locus_tag="CTI62_29165"
     CDS             1421..1867
                     /locus_tag="CTI62_RS29375"
                     /old_locus_tag="CTI62_29165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(2409..2651)
                     /locus_tag="CTI62_RS29385"
                     /old_locus_tag="CTI62_29175"
     CDS             complement(2409..2651)
                     /locus_tag="CTI62_RS29385"
                     /old_locus_tag="CTI62_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     gene            2834..3154
                     /locus_tag="CTI62_RS29390"
                     /old_locus_tag="CTI62_29180"
     CDS             2834..3154
                     /locus_tag="CTI62_RS29390"
                     /old_locus_tag="CTI62_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3358..3714
                     /locus_tag="CTI62_RS29395"
                     /old_locus_tag="CTI62_29185"
     CDS             3358..3714
                     /locus_tag="CTI62_RS29395"
                     /old_locus_tag="CTI62_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     Typing          complement(4015..4424)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttacactgag cactgacgct ctgaggcttc ttatgacgaa acaacagaaa acagcgctga
       61 acatggctaa attcatccag aatcagtctc tgctcctgct ggagaagctt aacgagcttg
      121 atctcgatgt tgaggccgac ctttgtgaga aactccacga tgatgcggag catcttttcc
      181 gtacactgtc ctcgagactg gatgaattac aggatggtaa ctgatggcca gagtgtctat
      241 tagtgaagcg gcacggcttg ttaaggttag tcgccccaca atttacaaga tgattaacag
      301 tgggaagtta agttacacct ctgttgtaaa gcacgggaag atcattaaag ttattgatac
      361 ttcagagctt attagagttt ttggttctct tgatggtgtc atcgatactg taaaaagtga
      421 cgcaggatct acaggtgtta acagcgttgg tttacatgat ttacaacata gaattgcttt
      481 actagaagct gaaaacgatg gtcttaaagg ggctgttaag gctcgagatg agcatattga
      541 ttcccttcgt caggctatgc agcttctgga gcacaagcac gaaccttctt caccacccga
      601 ttccccgtgg tggaaattct ggaaaaaatc ctgactccgt catgcttccg ttttcctctc
      661 cggccatccc ggggagttcc tgaaccttcc caaaaatcgc cagatctctg aacgctcctc
      721 accactaaaa atacccacac cccacgcaaa attaaaattt tgaagataat tcattaatat
      781 cagttagttg tgggttatta cattacgcag tattactttc tgtaaggtga atccaagtcg
      841 tcggcgattc gtgtacgact tgccgacgac tcgtcataga gtcatcgacg actcacttaa
      901 gactatcagt aacttatgac gacttgactc tagagtcgaa tcgtcatttt ggtgtttttt
      961 tgaccttggc gactctagag tcaagtcacg agtcgaatcg ccggtgaatc gtgtggtgag
     1021 tcgtccggcg acttgcataa cgtgattgcc ggccgtcgac gaacccgatt cctgaacgtg
     1081 ctgaaaaccg tttttccggc tgcgccggca acgcttcacg gccacgcaat aaattgcgtc
     1141 cccgttcacc gttttcagtc tggtttggtg cgggttttga gttgtctggc ctgaattcag
     1201 gctgaaatcc gccaccagtc agtctggtgc ataacaattc atgcccgtaa aacgccctgg
     1261 agcgattttg aggcgttttc agggggtaag acgtaggatt taatggatta cgccccagaa
     1321 tcgtttctag agcgtttgag cggtgcgcgt aatgaggcgt tatggtaaat aattgctcgt
     1381 gaaaacgtta ttaacatttt tatcttatga ggtgatgtga gtgaacgata aatcaggctt
     1441 tagttttatg aagatcggtg caggtgcaaa agtgactggt ttaagaatga aaaggaatgt
     1501 gatggttgtt gatgatctgt ctcacgtaga gagctttgtt actgttggtg agcgaggcga
     1561 agttattaat ctcgatgcgg atgggaacga actacacaca ccagacagtt atcgtgaaaa
     1621 gttaaaaata gaacgcggtg aactccaggc ttccttggag aaagccaagg aggaaatcaa
     1681 ctcaatagat aacgatataa cttcgcaaaa gattaagaac cagttgagtg ctgcactatc
     1741 tcaaccaatg gatgcagaag gtgagtggaa cactaagaga atcctacaag gggttttaga
     1801 tttatctaaa caagtgggct gtggtgtact cacggctgcg atcacgcatt atcttggagt
     1861 tagttaataa actacctatg cccccatggt gggtatacaa aaaccatggg gagcggagcg
     1921 accttcgtct agctcttttc ttgtgtgtct catcttgcaa cggattcgat ttgtgtgggg
     1981 ttagtgtcag acttcttggg gtcagtttgg atatagagaa ttattgtacg gtaagcctgt
     2041 gttttgaaca ggcttacctc tcgcaatgat gtactcccgc gctatacgcc gtttcccgtg
     2101 aattttcctt aatcggataa cctaaatgcg atacagaaag agcggtgact taaaagcgat
     2161 cgtagctgag gtaaaatcaa tcccttacgc atcaatattg gttaaataat gcgcttaacg
     2221 tacaaaagtt tccgatatcc aaactgaccc ctatttggcg gcttttctta ttttcttgat
     2281 tttcccgcat ctgaacgttt ctctcggact cttccattat tattacgatc tcgtggctga
     2341 gttccgcctc ctggtttagg gacgacgaca tgcgggttaa gtggtctacg gtctgccatt
     2401 ttcatacctc actctgcata aacaaatagt tgccagcgga tgtccccctg tccgggacta
     2461 tgttgatgat gattttgtgg tggaagtgtt ttccctcctg cgatagttac ttctttaccg
     2521 caacctgtgc atttgtaaat tccttcatac gtgcacgtat cgccaggctt gtgttgtgta
     2581 tcaaaaactg ggttaaattt cggatctgct tcaaaaatct tggttttatt tcctgagcga
     2641 acaagcgcca tatgacctcc aactattctt tttttgcaca gtagcactgt gcttttgtgc
     2701 attttatatc attatggttt tttgcacaag tgcacagtgc tattgtgcat ttctggcgtt
     2761 tgttgtacta tggtggtggt aaaaggtgat ctagaacttg ccaagttgcg gcaacatttg
     2821 aggtaaaaga aaaatgcagc aaaatcatga tagtgatgat gattgccgca ttcagttccg
     2881 agacgattgg ctataccagt tttataaata cggtaaagcc aatcgaaaaa ttccagctac
     2941 gattagtgtt tcattaggtc gcaagctgga tatgctgaat gctgctgata aaattggaga
     3001 tttacggtct cccccgggga accgatttga aaatctcaac cctccattga cagggtattc
     3061 atctataagg gtgaatgata agtaccgctt gatatttaag tggggtgaga acggagtcga
     3121 ggatttgtat ctcgatccac atgattaccg ataggtaaca gaccggaact gttatcttac
     3181 aaaacaagta ggtgaggcat ttcctcacct acgttaaaac gtcatcacaa ataatgatta
     3241 ataaagtaac gcgacgtaac tatatttaag ataattacta atcgatacag ctggtaacag
     3301 aagaaatact tatcttcctc atctaacaga gagaagcgac caattcagga gtaaagcatg
     3361 tcccaagcaa ccagaaaacc atcgactgta ggagatatta ttctttatga atatctcgag
     3421 ccaagtggtt taaaaatcaa tgacttggct gaaatgttaa acgtgcatag aaattctgca
     3481 agtgctttgg ttaacaataa taggaaactg tctaccgata tggcgtttcg gttagctaaa
     3541 gcattcgata ctaccgctaa gttctggtta aatatacagc agtctgttga tttgtgggag
     3601 gttacgagca gcccacgcct tcaggaagaa atagaaagag tcactcccgc tcatgaaatt
     3661 atagaaagga agcacaatgc tgaatctttg gaaaaaaaac atgcggtcgc atgatttttt
     3721 taaaaaaatt taaccaaact agaagaaggc ctctgtaata gtgtgccttc tttttttgta
     3781 accgctaaag gtcatcatca ttttctttta cgagcacttg agatccggtt tcggtacgcg
     3841 taatctcttg ctctgtaaac ggaaaaaccg ccttgcaggg cggtttttcc gaaggttcaa
     3901 acacttgcag gtgattgaac caaggtaact ggcttggagg agcgcagtca ccaaaacttg
     3961 ttctttcagt ttagccttaa caggcgcatg acttcaagac taactcctct aaatcagtta
     4021 ccagtggctg ctgccagtgg cgcttttgca tgtcttaccg ggttggactc aagacgatag
     4081 ttaccggata agacgcagca gtcggactga acggggggtt cgtgcataca gtccagcttg
     4141 gagcgaactg ccttcccgga actgagtgtc tggcgtggaa tgagataaac gcggccataa
     4201 cagcggaatg acaccggtaa accgaatggc aggaacagga gagcgcacga gggagccatc
     4261 agggggaaac gcctgatatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg
     4321 tcaatttcta tgatgcttgt caggggggcg gagcctatgg aaaaacggcc gctaagcggc
     4381 ctttccttct gcccatcacg cgtccgctcg gttttctcga gcatttatac gcatagtcct
     4441 accgctcgcc gcagccgaac ggcaggagcg tagcgactga gtgagcgagg aagcggaaaa
     4501 gagactggtt
//
