LOCUS       NZ_CP024533             4510 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF157 isolate INF157 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP024533
VERSION     NZ_CP024533.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357077
            Assembly: GCF_002753075.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: INF157
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:16:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,605
            Genes (coding)                    :: 5,446
            CDSs (with protein)               :: 5,446
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 50 of 159
            Pseudo Genes (incomplete)         :: 108 of 159
            Pseudo Genes (internal stop)      :: 16 of 159
            Pseudo Genes (multiple problems)  :: 15 of 159
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024533.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF157"
                     /isolate="INF157"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Australia"
                     /collection_date="2013-08"
     gene            complement(141..551)
                     /locus_tag="CTI61_RS29750"
                     /old_locus_tag="CTI61_29545"
     CDS             complement(141..551)
                     /locus_tag="CTI61_RS29750"
                     /old_locus_tag="CTI61_29545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(551..742)
                     /locus_tag="CTI61_RS29755"
                     /old_locus_tag="CTI61_29550"
     CDS             complement(551..742)
                     /locus_tag="CTI61_RS29755"
                     /old_locus_tag="CTI61_29550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1571..1927)
                     /locus_tag="CTI61_RS29770"
                     /old_locus_tag="CTI61_29565"
     CDS             complement(1571..1927)
                     /locus_tag="CTI61_RS29770"
                     /old_locus_tag="CTI61_29565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(2131..2451)
                     /locus_tag="CTI61_RS29775"
                     /old_locus_tag="CTI61_29570"
     CDS             complement(2131..2451)
                     /locus_tag="CTI61_RS29775"
                     /old_locus_tag="CTI61_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2634..2876
                     /locus_tag="CTI61_RS29780"
                     /old_locus_tag="CTI61_29575"
     CDS             2634..2876
                     /locus_tag="CTI61_RS29780"
                     /old_locus_tag="CTI61_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     gene            complement(3418..3864)
                     /locus_tag="CTI61_RS29785"
                     /old_locus_tag="CTI61_29585"
     CDS             complement(3418..3864)
                     /locus_tag="CTI61_RS29785"
                     /old_locus_tag="CTI61_29585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     Typing          862..1271
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaattatc ttcaaaattt taattttgcg tggggtgtgg gtatttttag tggtgaggag
       61 cgttcagaga tctggcgatt tttgggaagg ttcaggaact ccccgggatg gccggagagg
      121 aaaacggaag catgacggag tcaggatttt ttccagaatt tccaccacgg ggaatcgggt
      181 ggtgaagaag gttcgtgctt gtgctccaga agctgcatag cctgacgaag ggaatcaata
      241 tgctcatctc gagccttaac agccccttta agaccatcgt tttcagcttc tagtaaagca
      301 attctatgtt gtaaatcatg taaaccaacg ctgttaacac ctgtagatcc tgcgtcactt
      361 tttacagtat cgatgacacc atcaagagaa ccaaaaactc taataagctc tgaagtatca
      421 ataactttaa tgatcttccc gtgctttaca acagaggtgt aacttaactt cccactgtta
      481 atcatcttgt aaattgtggg gcgactaacc ttaacaagcc gtgccgcttc actaatagac
      541 actctggcca tcagttacca tcctgtaatt catccagtct cgaggacagt gtacggaaaa
      601 gatgctccgc atcatcgtgg agtttctcac aaaggtcggc ctcaacatcg agatcaagct
      661 cgttaagctt ctccagcagg agcagagact gattctggat gaatttagcc atgttcagcg
      721 ctgttttctg ttgtttcgtc ataagaagcc tcagagcgtc agtgctcagt gtaaaaccag
      781 tctcttttcc gcttcctcgc tcactcagtc gctacgctcc tgccgttcgg ctgcggcgag
      841 cggtaggact atgcgtataa atgctcgaga aaaccgagcg gacgcgtgat gggcagaagg
      901 aaaggccgct tagcggccgt ttttccatag gctccgcccc cctgacaagc atcatagaaa
      961 ttgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatatc aggcgtttcc
     1021 ccctgatggc tccctcgtgc gctctcctgt tcctgccatt cggtttaccg gtgtcattcc
     1081 gctgttatgg ccgcgtttat ctcattccac gccagacact cagttccggg aaggcagttc
     1141 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgactgctgc gtcttatccg
     1201 gtaactatcg tcttgagtcc aacccggtaa gacatgcaaa agcgccactg gcagcagcca
     1261 ctggtaactg atttagagga gttagtcttg aagtcatgcg cctgttaagg ctaaactgaa
     1321 agaacaagtt ttggtgactg cgctcctcca agccagttac cttggttcaa tcacctgcaa
     1381 gtgtttgaac cttcggaaaa accgccctgc aaggcggttt ttccgtttac agagcaagag
     1441 attacgcgta ccgaaaccgg atctcaagtg ctcgtaaaag aaaatgatga tgacctttag
     1501 cggttacaaa aaaagaaggc acactattac agaggccttc ttctagtttg gttaaatttt
     1561 tttaaaaaaa tcatgcgacc gcatgttttt tttccaaaga ttcagcattg tgcttccttt
     1621 ctataatttc atgagcggga gtgactcttt ctatttcttc ctgaaggcgt gggctgctcg
     1681 taacctccca caaatcaaca gactgctgta tatttaacca gaacttagcg gtagtatcga
     1741 atgctttagc taaccgaaac gccatatcgg tagacagttt cctattattg ttaaccaaag
     1801 cacttgcaga atttctatgc acgtttaaca tttcagccaa gtcattgatt tttaaaccac
     1861 ttggctcgag atattcataa agaataatat ctcctacagt cgatggtttt ctggttgctt
     1921 gggacatgct ttactcctga attggtcgct tctctctgtt agatgaggaa gataagtatt
     1981 tcttctgtta ccagctgtat cgattagtaa ttatcttaaa tatagttacg tcgcgttact
     2041 ttattaatca ttatttgtga tgacgtttta acgtaggtga ggaaatgcct cacctacttg
     2101 ttttgtaaga taacagttcc ggtctgttac ctatcggtaa tcatgtggat cgagatacaa
     2161 atcctcgact ccgttctcac cccacttaaa tatcaagcgg tacttatcat tcacccttat
     2221 agatgaatac cctgtcaatg gagggttgag attttcaaat cggttccccg ggggagaccg
     2281 taaatctcca attttatcag cagcattcag catatccagc ttgcgaccta atgaaacact
     2341 aatcgtagct ggaatttttc gattggcttt accgtattta taaaactggt atagccaatc
     2401 gtctcggaac tgaatgcggc aatcatcatc actatcatga ttttgctgca tttttctttt
     2461 acctcaaatg ttgccgcaac ttggcaagtt ctagatcacc ttttaccacc accatagtac
     2521 aacaaacgcc agaaatgcac aatagcactg tgcacttgtg caaaaaacca taatgatata
     2581 aaatgcacaa aagcacagtg ctactgtgca aaaaaagaat agttggaggt catatggcgc
     2641 ttgttcgctc aggaaataaa accaagattt ttgaagcaga tccgaaattt aacccagttt
     2701 ttgatacaca acacaagcct ggcgatacgt gcacgtatga aggaatttac aaatgcacag
     2761 gttgcggtaa agaagtaact atcgcaggag ggaaaacact tccaccacaa aatcatcatc
     2821 aacatagtcc cggacagggg gacatccgct ggcaactatt tgtttatgca gagtgaggta
     2881 tgaaaatggc agaccgtaga ccacttaacc cgcatgtcgt cgtccctaaa ccaggaggcg
     2941 gaactcagcc acgagatcgt aataataatg gaagagtccg agagaaacgt tcagatgcgg
     3001 gaaaatcaag aaaataagaa aagccgccaa ataggggtca gtttggatat cggaaacttt
     3061 tgtacgttaa gcgcattatt taaccaatat tgatgcgtaa gggattgatt ttacctcagc
     3121 tacgatcgct tttaagtcac cgctctttct gtatcgcatt taggttatcc gattaaggaa
     3181 aattcacggg aaacggcgta tagcgcggga gtacatcatt gcgagaggta agcctgttca
     3241 aaacacaggc ttaccgtaca ataattctct atatccaaac tgaccccaag aagtctgaca
     3301 ctaaccccac acaaatcgaa tccgttgcaa gatgagacac acaagaaaag agctagacga
     3361 aggtcgctcc gctccccatg gtttttgtat acccaccatg ggggcatagg tagtttatta
     3421 actaactcca agataatgcg tgatcgcagc cgtgagtaca ccacagccca cttgtttaga
     3481 taaatctaaa accccttgta ggattctctt agtgttccac tcaccttctg catccattgg
     3541 ttgagatagt gcagcactca actggttctt aatcttttgc gaagttatat cgttatctat
     3601 tgagttgatt tcctccttgg ctttctccaa ggaagcctgg agttcaccgc gttctatttt
     3661 taacttttca cgataactgt ctggtgtgtg tagttcgttc ccatccgcat cgagattaat
     3721 aacttcgcct cgctcaccaa cagtaacaaa gctctctacg tgagacagat catcaacaac
     3781 catcacattc cttttcattc ttaaaccagt cacttttgca cctgcaccga tcttcataaa
     3841 actaaagcct gatttatcgt tcactcacat cacctcataa gataaaaatg ttaataacgt
     3901 tttcacgagc aattatttac cataacgcct cattacgcgc accgctcaaa cgctctagaa
     3961 acgattctgg ggcgtaatcc attaaatcct acgtcttacc ccctgaaaac gcctcaaaat
     4021 cgctccaggg cgttttacgg gcatgaattg ttatgcacca gactgactgg tggcggattt
     4081 cagcctgaat tcaggccaga caactcaaaa cccgcaccaa accagactga aaacggtgaa
     4141 cggggacgca atttattgcg tggccgtgaa gcgttgccgg cgcagccgga aaaacggttt
     4201 tcagcacgtt caggaatcgg gttcgtcgac ggccggcaat cacgttatgc aagtcgccgg
     4261 acgactcacc acacgattca ccggcgattc gactcgtgac ttgactctag agtcgccaag
     4321 gtcaaaaaaa caccaaaatg acgattcgac tctagagtca agtcgtcata agttactgat
     4381 agtcttaagt gagtcgtcga tgactctatg acgagtcgtc ggcaagtcgt acacgaatcg
     4441 ccgacgactt ggattcacct tacagaaagt aatactgcgt aatgtaataa cccacaacta
     4501 actgatatta
//
