LOCUS       NZ_CP024532             4660 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF157 isolate INF157 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP024532
VERSION     NZ_CP024532.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357077
            Assembly: GCF_002753075.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: INF157
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:16:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,605
            Genes (coding)                    :: 5,446
            CDSs (with protein)               :: 5,446
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 50 of 159
            Pseudo Genes (incomplete)         :: 108 of 159
            Pseudo Genes (internal stop)      :: 16 of 159
            Pseudo Genes (multiple problems)  :: 15 of 159
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024532.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF157"
                     /isolate="INF157"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Australia"
                     /collection_date="2013-08"
     gene            587..778
                     /locus_tag="CTI61_RS29715"
                     /old_locus_tag="CTI61_29515"
     CDS             587..778
                     /locus_tag="CTI61_RS29715"
                     /old_locus_tag="CTI61_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            778..1179
                     /locus_tag="CTI61_RS29720"
                     /old_locus_tag="CTI61_29520"
     CDS             778..1179
                     /locus_tag="CTI61_RS29720"
                     /old_locus_tag="CTI61_29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2022..2231)
                     /locus_tag="CTI61_RS29730"
                     /old_locus_tag="CTI61_29525"
     CDS             complement(2022..2231)
                     /locus_tag="CTI61_RS29730"
                     /old_locus_tag="CTI61_29525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            2619..2801
                     /locus_tag="CTI61_RS29735"
                     /old_locus_tag="CTI61_29530"
     CDS             2619..2801
                     /locus_tag="CTI61_RS29735"
                     /old_locus_tag="CTI61_29530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080882243.1"
                     /translation="MGKELSLNKIYDKNSRYINDSRGHELGKEKKVFDFEVEMIKKRRE
                     DAIRKIIEDSKSLKW"
                     /name="hypothetical protein"
     gene            2814..4514
                     /locus_tag="CTI61_RS29740"
                     /old_locus_tag="CTI61_29535"
     CDS             2814..4514
                     /locus_tag="CTI61_RS29740"
                     /old_locus_tag="CTI61_29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697690.1"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage dCTP deaminase"
                     /protein_id="WP_225368539.1"
                     /translation="MSLARKLREKRSNPIHSANEDSSGGDSINQITSRQSMDLVIGFCG
                     AVGSGIKSIVDVTEEELDDLGYDVYRIRVSELMQKLFSEWRDDIVTPFDRYHKLQDFGD
                     ALRNKYYPHILSEAVIHEIKIEKGIDTSSKKNKKAFLVDQLKHQDEIELLRMVYQHNFY
                     LLGVIRSEVERKRNLRDEGLSKSEVDIVIHHDRKSESDYGQQTAKAILDSDYFVKNNQG
                     QRAHLKAKVKRFIGLIHGLNGLTPTTYEKGMYAAHSASLQSACLSRQVGAAILDVNGNL
                     ISVGRNDVPKFNGGLYSADDGVNDHRCVYKGGKCYNEVRKLKIKSKISQILMSESACEL
                     GVKLTEDQAEQLADVIYKKTPVSSIIEYSRSIHAEMDAITSLARLGNGGFSDKVLYTTT
                     FPCHNCARHIVAVGITKVVYIEPYEKSLALELHDDAITEVNEHGKVIFESFEGVSPRRY
                     QKFFFSTDERKDSFGNAEKYSTKYKNHIDIQFIDSYLDFENRVVDIFRTNTNIDSGNHV
                     GRTDSTINLSSQPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPDSK"
                     /name="anti-phage dCTP deaminase"
     Typing          complement(59..466)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.355"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cttgttcttt cagtttagcc ttaactggcg cataacttca agactaactc ctctaaatca
       61 gttaccggtg gctgctgcca gtggtgcttt tgcatgtctt accgggttgg actcaagatg
      121 atagttaccg gataagacgc agcagtcgga ctgaacgggg ggttcgtgca tacagtccag
      181 cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc
      241 ataacagcgg aatgacaccg gtaaaccgaa tggcaggaac aggagagcgc acgagggagc
      301 catcaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccgc cactgatttg
      361 agcgtcagat tccgtgatgc tcgtcagggg ggcggagcct atggaaaaac ggccgctgtg
      421 cggcctttcc ttttccccat cacgagaccg ctcggttttc tcgagctttt acacgcatgc
      481 tcctgccgct cgccgcagcc gaacggcagg agcgtagcga ctgagtgagc gaggaagcgg
      541 aaaagagact gttcttacac tgagcactga cgctctgagg cttcttatga cgaaacaaca
      601 gaaaacagcg ctgaacatgg ctaaattcat ccagaatcag tctctgctcc tgctggagaa
      661 gcttaacgag cttgatctcg atgttgaggc cgacctttgt gagaaactcc acgatgatgc
      721 ggagcatctt ttccgtacac tgtcctcgag actggatgaa ttacaggatg gtaactgatg
      781 gccagagtgt ctattagtga ggctgcccga ctgacgggta agagtagaac aacactgcac
      841 aggttgatta aagcaggtga cctgtccacc tgtagcggtg aacgcaatgc aaaaatggta
      901 gatacttcag agcttttacg tgtctttggt ccctttgagc aaccgaaggg tgaacaccat
      961 tctggacagg tctctgaaca gcgtgataca gcctctgctg accagagtga acaggtgatt
     1021 cgtcatctga aacaggagat tgaacacctg aataccttac ttttgtccaa ggattcgcac
     1081 attgacagcc tgaaacaggc catgctcctt ctcgaaaatc accaggcaaa attaactgaa
     1141 tccccggcgc catggtggaa attctggaaa aaatcctgac cccgtcatgc ttccgttttc
     1201 ctctccggcc atcccgggga gttcctgaac cttcccaaaa atcgtcagat ctctgaacgc
     1261 tcctcaccac taaaaatacc cacaccccac gcaaaattaa tattttgcag atttgtattt
     1321 aatatcagtg ggttactttc ctgaaatttc gttttttaaa ttttatttat atagatccga
     1381 ctctatgacg actcgtcata gagtccccgg cgagtcgtca tagagtcggt aacgactcag
     1441 gactataaaa acaataagtt aataacgacc cgactctaga cacgactccc cgtttggttg
     1501 ttttttagac gcggcgactc tgggtacgac tctagtgaag agtcgccggt gaatcgtgtg
     1561 gtgagtcgtc cggcgacttg ccataacgtg attgccggcc gtcgacgaac ccgattcctg
     1621 aacgtgctga aaaccggttt tccggctgcg ccggcaacgc ttcacggcca cgcaataaat
     1681 tgcgtccccg ttcaccgttt tcggatggaa agaacgccgg aaacaggact tactctggct
     1741 gaatggcgtg aattttcgcg ctatgcactt gcgtgcatac tcatgcatgc cgtaaaaaca
     1801 gagcctgcgc gtttctggcg ggttttcggg tggtttgttg cctgttttac cggttttccg
     1861 tcagaaacgc cctgaggccg ttttagcggt gcgtacaatc atgcctttat gttaaataac
     1921 agatgcaaaa aagccccaga aggggcctga aggttttcct gctaagggac gaaacgggcc
     1981 gcctcccggc ggcctgttcg gcagctccct ttgcgaccat cttatttggt cagttcagcc
     2041 gtcatgtgga cacggttatt gaactgagcg ctggtgattt tgtatgacgc accttgttca
     2101 gcggcctgtg cggcaatttt tgcttcagca cgatccaggg tggaggcggt agcgctgacg
     2161 ctctgagcaa aagcaccgaa agacatcatg gatagagcgg caactgcaac gaaagttttg
     2221 atggatttca tggtcgtatt ccttaagtga ttttgttctg tatggggcgt tgttgccctg
     2281 atgtgataaa tgataggacg accattagtt gattaaaatc gaaacaattt gcggatatca
     2341 ttcaaaaaaa aagaacaaga attgagcgca tcaagaccag aggtttatct ctggatttta
     2401 ggtaccaaat gaggtggtgg atagaaatgt taacattgat gattttattt taacaaattg
     2461 aaaaataatg gttagtttgg cgtaatctct cttgcttttc tcttgccgaa tttcctaatg
     2521 tttaggataa gaaaaaaaaa tctctatcga ttacgtttct tggctattgc ctgttataaa
     2581 gataacagta aaatccatgc gtcttgcagg agggtgtgat gggtaaagag ctatctctaa
     2641 ataaaattta tgataagaat tctagatata ttaatgattc tagagggcat gagctgggaa
     2701 aggaaaagaa agttttcgat tttgaagttg aaatgattaa aaaaagaaga gaggacgcta
     2761 taaggaagat aattgaggat tcaaaatcat taaaatggta agtgaggtct tttatgagcc
     2821 tggctagaaa gttaagagaa aaaagaagta atccgattca tagtgctaac gaggactcct
     2881 ctggagggga tagcataaac caaataacct ccagacagtc tatggatctt gttataggat
     2941 tttgcggtgc tgttggctca ggaataaagt caattgttga tgtgactgaa gaagaactag
     3001 atgatctagg atatgatgtt tatcgcataa gagttagcga gcttatgcaa aaactttttt
     3061 cagaatggcg tgatgatatt gttactccgt ttgatagata tcataagctg caagattttg
     3121 gtgatgcgtt aagaaataaa tattacccac atatactatc tgaagctgta atccatgaaa
     3181 taaaaataga aaaaggcatt gacacctcaa gtaaaaaaaa caaaaaggct tttttggtcg
     3241 atcagttgaa gcatcaggat gaaattgaac ttttaaggat ggtctaccaa cataattttt
     3301 atttactagg tgtaattaga agtgaagtag aaagaaagcg taatcttaga gatgaagggc
     3361 tgagtaagtc agaagtcgat atagttattc atcacgatag aaaatcagag agcgattatg
     3421 gtcagcagac tgcgaaagct atacttgact ctgattattt tgttaagaat aatcaaggac
     3481 aaagggcgca ccttaaggct aaggttaagc gttttattgg cttgatacat gggctaaatg
     3541 gtttaactcc aaccacgtat gaaaaaggta tgtatgcagc tcattcagca tctctgcaat
     3601 ctgcatgcct ttctcgtcaa gttggtgctg ctatattgga tgttaatggg aatctgattt
     3661 ccgttgggcg taatgatgta cctaaattca atggtgggct ttatagtgcg gatgatggtg
     3721 tcaatgatca tcgttgtgta tacaaaggtg gtaaatgtta taatgaagtt agaaaactaa
     3781 agattaaaag caaaatttca caaatactta tgtcggaaag cgcttgtgaa cttggtgtta
     3841 aattaacaga agatcaagca gagcaactgg ctgatgtaat atataaaaaa acaccagtat
     3901 cttctattat agagtactct cgctcaattc atgcagagat ggatgctatt acatcattag
     3961 ctagactagg taatggaggt ttttctgata aagttttata tacaaccaca tttccttgtc
     4021 ataactgcgc gagacatatt gtcgctgttg gaataacaaa agttgtttat atagagccat
     4081 atgaaaaaag cctcgcttta gaattgcatg atgatgctat aaccgaggtg aatgagcatg
     4141 ggaaggttat atttgaatcc tttgaaggtg tatcacctcg taggtatcaa aaattcttct
     4201 tttctacaga tgaaaggaaa gatagtttcg gtaacgcaga aaagtactcc actaaatata
     4261 aaaatcacat tgatatacag tttattgata gctatttaga tttcgaaaat cgagtcgttg
     4321 atatttttag aactaacaca aatattgatt ctggcaatca tgtcgggaga actgattcga
     4381 ctattaattt atcttcccag cccgtaccgc ccgtaccgcc cgtaccgccc gtaccgcccg
     4441 taccgccagt accgccagta ccgccagtac cgccagtacc accagtacca ccagtaccac
     4501 cagactcgaa atgaagatct gttgactctg atgttataca gagcttaacc ttgagacgag
     4561 aaagaaaaaa accgcctttg cggggcggtt tttttaaggt tctagagttt gcggctctat
     4621 gaaccaaggt aactggctta gaggagcgca gtcaccaaaa
//
