LOCUS       NZ_CP024527             3825 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF158 isolate INF158 plasmid unnamed6,
            complete sequence.
ACCESSION   NZ_CP024527
VERSION     NZ_CP024527.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357080
            Assembly: GCF_002753055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: INF158
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 125x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:23:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,605
            Genes (coding)                    :: 5,446
            CDSs (with protein)               :: 5,446
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 50 of 159
            Pseudo Genes (incomplete)         :: 108 of 159
            Pseudo Genes (internal stop)      :: 16 of 159
            Pseudo Genes (multiple problems)  :: 15 of 159
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024527.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF158"
                     /isolate="INF158"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="Australia"
                     /collection_date="2013-08"
     gene            join(3779..3825,1..145)
                     /locus_tag="CTI59_RS29785"
                     /old_locus_tag="CTI59_29595"
     CDS             join(3779..3825,1..145)
                     /locus_tag="CTI59_RS29785"
                     /old_locus_tag="CTI59_29595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            145..546
                     /locus_tag="CTI59_RS29790"
                     /old_locus_tag="CTI59_29600"
     CDS             145..546
                     /locus_tag="CTI59_RS29790"
                     /old_locus_tag="CTI59_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            1351..1623
                     /locus_tag="CTI59_RS29800"
                     /old_locus_tag="CTI59_29610"
     CDS             1351..1623
                     /locus_tag="CTI59_RS29800"
                     /old_locus_tag="CTI59_29610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019405140.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     Typing          2943..3157
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.562"
                     /coverage="100.0"
                     /evalue="1.62e-49"
     Typing          complement(2446..2962)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.847"
                     /coverage="87.0"
                     /evalue="5.5e-153"
ORIGIN
        1 gaatcagtct ctgctcctgc tggagaagct taacgagctt gatctcgatg ttgaggcgga
       61 cctttgtgag aaactccacg atgatgcgga gcatcttttc cgtacactgt cctcgagact
      121 ggatgcatta caggatggta actgatggcc agagtgtcta ttagtgaggc tgcccgactg
      181 acgggtaaga gtagaacaac actgcacagg ttaattaaag caggtgacct gtccacctgt
      241 agcggtgaac gcaatgcaaa aatggtagat acttcagagc ttttacgtgt ctttggtccc
      301 tttgaacaac cgaagggtga acaccattct ggacaggtct ctggacagcg tgatacagcc
      361 tctgctgacc agagtgaaca ggtgattcgt catctgaaac aggagattga acacctgaac
      421 accttacttt tgtccaagga ttcgcacatt gacagcctga aacaggccat gctccttctt
      481 gaaaatcacc aggcaaaatt aactgaatcc ccggcgccat ggtggaaatt ctggaaaaaa
      541 tcctgatccc gccatgcttc cattttgctc tccggccatt ccggggagtt cctgaacctt
      601 cccaaaaatc gtcagatccc tgaacgctcc tcaccactaa aaatacccac acactacgca
      661 aaattaaaat tttgcagata gacctttatt ttcaatcagt tatctgtttt aaattacgca
      721 attttatttt ttgtaaggtg aatccgagtc gtggacgact ctatggcgaa tcgccgatga
      781 cttgcccacg attcatatgt gaatcacgcc atataatcag tacgttatga tgacttgact
      841 ctagagtcga cttgccgggt tggtggcttt ttgaccatgg cgactctaga gtcaagtcac
      901 gagtcgaatc gccggtgaat tgtctggtga gtcgtccggc gacttgccat aacgtgattg
      961 ccggccgtcg acgaacccga ttcctgatcg tgctgaaaac cggttttccg gctgcgccgg
     1021 caacgcttca cggccacgca ataaattgcg tccccgttca ccgttttcgg ataggaagaa
     1081 cgccggaaac aggacttacc ctggctgaat ggcgtgaatt tccgcgctat gcacttgcgt
     1141 gcatactcat gcatgccgta aaaacagagc cagcgcgttt ctggcggatt ttcgggtggt
     1201 ttgttgcctg ttttaccggt ttcccgtcag gaacgccctg aggccgtttt cgcggtgcgc
     1261 gtaatgggac gttatggtaa atcaacggcg gatgcctgag tctgtgttag agtcagcgga
     1321 ttgtgataat cagtgactca gggagatgtt ttggctactg acgctcaggt aaaagaaatt
     1381 aatgcactca tcaaaaaata cccggactca tgctccatct gtcatgaagt ctatgatgag
     1441 gatgatgtga cctacaccgt ttttgggtat gaccggaaag gtaaaataca ggtcaccacg
     1501 ggatgctgtg ccggaatgct gacagagccg gttctgctgg gtgtctgtgg gtgctttgac
     1561 ccggaagaac gtgacgagat catgcagaat caccccatgg caaagcagtt tttcacagag
     1621 tgattccgta tctgttgagt ctgaagcccg ccttgtgcgg gctttttcat atctgcgtga
     1681 aatgagacgt tatggtaaat ctctaaaggc aatatttccc gtcccttgga gcgaactact
     1741 ctgtatttga tctttggtgt tgataccccg ctgcttgcag aggggggatt cattatcgtt
     1801 gttgtttgct gtaattgtct aaaatatatt tggggataga tacgggtaca cattcagcag
     1861 atattatgtt tttcccggat atattatgat aaatgtcttt gtttgcaatg attttatctt
     1921 tgaattcaat gcattcttca ttcagaatga aggtgttaac aacattgtct ttcattccca
     1981 aatttgctaa ttctgaagaa caacttgcaa tattattcgt gttaaaacaa gttgttagta
     2041 ttattgcgta tatgtatggc attaaataaa cctatttaaa aaagtacgga atgatagtga
     2101 tcttgttcgg aaatatacta gtcagacttt aagtctcagg gatatgaacc tagaacatcg
     2161 ttgtattaac ttgtttgagt gttccaatcc tgaatattga tgttatttga gtataaagga
     2221 aacataataa atatcacaag gatattggtt aactatttat ttttaaataa tttcttgaga
     2281 tctgacgttg gacgtcgtag tctcttgcca tgtaaacgaa aaaaccacct tgcagggtgg
     2341 tttttcgaag gttcagtaaa ccggcaagtt tctgaaccgt ggtaacagtc ttgtgcgaga
     2401 cgtcaccaaa tctgtccttt tagtgtagcc tcagtctggc caccacttca agaactcttc
     2461 gtaaatctct cgcacatcct gtttaccagt ggccgctgcc agtggcgtta agtcgtgtct
     2521 ttccgggttg gactcaagac gatagttacc ggaaaaggcg cagcggtcgg gctgaacggg
     2581 gggttcttgc atacagccca gcttggagcg aactgtctaa acggaacggg gcgtggtgat
     2641 ttaggtaaag cttccaccac tacacggatg ccgcaggacg ggaacaggag agcgcaagag
     2701 ggagccacca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg
     2761 atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt
     2821 tgccgcggcc ttatcgtatc cctgttaagt ttcttcctgg catccaccag gaaataaatt
     2881 tccgccccat tagtaagcct gcaccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2941 agtgagcgag gaagcggaat atcacctgaa ccagcatctt ctcctgacgc cggaaagcct
     3001 catttcactc ctgccacatg aagcactttg gcaaatcacc acttcggttt aaaatattta
     3061 gccagtatgc actccgctag cgctacgtga ctggttcagg gctgcgcccc gaaacccgct
     3121 aacacccctc agctactgga tatcacacca tgccatctga gtatgatgaa caaccggatg
     3181 cgactgatgc tctgtttccc ggcgaatatg acctttttga cttaatggct catgttactg
     3241 aagagaacct gcacgccccg gtggattctg gcccgcctgt cggccgggaa gcgttatagc
     3301 tttttcgctg gccagcgtac atgtaatacg ctggtattac atgttatttg tttattttca
     3361 tgttgttaca gatgattgta atacgttgcg tattacgcta atacacgaaa taactccatt
     3421 tttttcattt aattcatgca gttgcgataa aaatcgcggc tgcgttaggt gtatgaccat
     3481 ttaaggggca aaaaattatc attctggctg ggtgatctgc gcaggttaag gatcatcggc
     3541 gacgaaaaag cgtctttaac tgcttcgttc atgcttgcat gaacggatta tttttccaga
     3601 ctacaggtaa actacaggaa gactacaaaa gttgatgatt taatcctgcg ttttagtgcg
     3661 atgactacag ataaactaca aaataactac aggataacct cagatccgtt ctctgaacca
     3721 ttcgtacatc cgaccccctt accggtttac actgagcact gatgctctga gggcccttat
     3781 gacgaaacaa cagaaaacag cgctgaacat ggctaaattc atcca
//
