LOCUS       NZ_CP024526             4510 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF158 isolate INF158 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP024526
VERSION     NZ_CP024526.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357080
            Assembly: GCF_002753055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: INF158
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 125x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:23:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,728
            CDSs (total)                      :: 5,605
            Genes (coding)                    :: 5,446
            CDSs (with protein)               :: 5,446
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 50 of 159
            Pseudo Genes (incomplete)         :: 108 of 159
            Pseudo Genes (internal stop)      :: 16 of 159
            Pseudo Genes (multiple problems)  :: 15 of 159
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024526.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF158"
                     /isolate="INF158"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Australia"
                     /collection_date="2013-08"
     gene            join(4354..4510,1..35)
                     /locus_tag="CTI59_RS29745"
                     /old_locus_tag="CTI59_29590"
     CDS             join(4354..4510,1..35)
                     /locus_tag="CTI59_RS29745"
                     /old_locus_tag="CTI59_29590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            35..445
                     /locus_tag="CTI59_RS29750"
                     /old_locus_tag="CTI59_29550"
     CDS             35..445
                     /locus_tag="CTI59_RS29750"
                     /old_locus_tag="CTI59_29550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            1232..1678
                     /locus_tag="CTI59_RS29755"
                     /old_locus_tag="CTI59_29555"
     CDS             1232..1678
                     /locus_tag="CTI59_RS29755"
                     /old_locus_tag="CTI59_29555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(2220..2462)
                     /locus_tag="CTI59_RS29760"
                     /old_locus_tag="CTI59_29565"
     CDS             complement(2220..2462)
                     /locus_tag="CTI59_RS29760"
                     /old_locus_tag="CTI59_29565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     gene            2645..2965
                     /locus_tag="CTI59_RS29765"
                     /old_locus_tag="CTI59_29570"
     CDS             2645..2965
                     /locus_tag="CTI59_RS29765"
                     /old_locus_tag="CTI59_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3169..3525
                     /locus_tag="CTI59_RS29770"
                     /old_locus_tag="CTI59_29575"
     CDS             3169..3525
                     /locus_tag="CTI59_RS29770"
                     /old_locus_tag="CTI59_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     Typing          complement(3826..4235)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cctcgagact ggatgaatta caggatggta actgatggcc agagtgtcta ttagtgaagc
       61 ggcacggctt gttaaggtta gtcgccccac aatttacaag atgattaaca gtgggaagtt
      121 aagttacacc tctgttgtaa agcacgggaa gatcattaaa gttattgata cttcagagct
      181 tattagagtt tttggttctc ttgatggtgt catcgatact gtaaaaagtg acgcaggatc
      241 tacaggtgtt aacagcgttg gtttacatga tttacaacat agaattgctt tactagaagc
      301 tgaaaacgat ggtcttaaag gggctgttaa ggctcgagat gagcatattg attcccttcg
      361 tcaggctatg cagcttctgg agcacaagca cgaaccttct tcaccacccg attccccgtg
      421 gtggaaattc tggaaaaaat cctgactccg tcatgcttcc gttttcctct ccggccatcc
      481 cggggagttc ctgaaccttc ccaaaaatcg ccagatctct gaacgctcct caccactaaa
      541 aatacccaca ccccacgcaa aattaaaatt ttgaagataa ttcattaata tcagttagtt
      601 gtgggttatt acattacgca gtattacttt ctgtaaggtg aatccaagtc gtcggcgatt
      661 cgtgtacgac ttgccgacga ctcgtcatag agtcatcgac gactcactta agactatcag
      721 taacttatga cgacttgact ctagagtcga atcgtcattt tggtgttttt ttgaccttgg
      781 cgactctaga gtcaagtcac gagtcgaatc gccggtgaat cgtgtggtga gtcgtccggc
      841 gacttgcata acgtgattgc cggccgtcga cgaacccgat tcctgaacgt gctgaaaacc
      901 gtttttccgg ctgcgccggc aacgcttcac ggccacgcaa taaattgcgt ccccgttcac
      961 cgttttcagt ctggtttggt gcgggttttg agttgtctgg cctgaattca ggctgaaatc
     1021 cgccaccagt cagtctggtg cataacaatt catgcccgta aaacgccctg gagcgatttt
     1081 gaggcgtttt cagggggtaa gacgtaggat ttaatggatt acgccccaga atcgtttcta
     1141 gagcgtttga gcggtgcgcg taatgaggcg ttatggtaaa taattgctcg tgaaaacgtt
     1201 attaacattt ttatcttatg aggtgatgtg agtgaacgat aaatcaggct ttagttttat
     1261 gaagatcggt gcaggtgcaa aagtgactgg tttaagaatg aaaaggaatg tgatggttgt
     1321 tgatgatctg tctcacgtag agagctttgt tactgttggt gagcgaggcg aagttattaa
     1381 tctcgatgcg gatgggaacg aactacacac accagacagt tatcgtgaaa agttaaaaat
     1441 agaacgcggt gaactccagg cttccttgga gaaagccaag gaggaaatca actcaataga
     1501 taacgatata acttcgcaaa agattaagaa ccagttgagt gctgcactat ctcaaccaat
     1561 ggatgcagaa ggtgagtgga acactaagag aatcctacaa ggggttttag atttatctaa
     1621 acaagtgggc tgtggtgtac tcacggctgc gatcacgcat tatcttggag ttagttaata
     1681 aactacctat gcccccatgg tgggtataca aaaaccatgg ggagcggagc gaccttcgtc
     1741 tagctctttt cttgtgtgtc tcatcttgca acggattcga tttgtgtggg gttagtgtca
     1801 gacttcttgg ggtcagtttg gatatagaga attattgtac ggtaagcctg tgttttgaac
     1861 aggcttacct ctcgcaatga tgtactcccg cgctatacgc cgtttcccgt gaattttcct
     1921 taatcggata acctaaatgc gatacagaaa gagcggtgac ttaaaagcga tcgtagctga
     1981 ggtaaaatca atcccttacg catcaatatt ggttaaataa tgcgcttaac gtacaaaagt
     2041 ttccgatatc caaactgacc cctatttggc ggcttttctt attttcttga ttttcccgca
     2101 tctgaacgtt tctctcggac tcttccatta ttattacgat ctcgtggctg agttccgcct
     2161 cctggtttag ggacgacgac atgcgggtta agtggtctac ggtctgccat tttcatacct
     2221 cactctgcat aaacaaatag ttgccagcgg atgtccccct gtccgggact atgttgatga
     2281 tgattttgtg gtggaagtgt tttccctcct gcgatagtta cttctttacc gcaacctgtg
     2341 catttgtaaa ttccttcata cgtgcacgta tcgccaggct tgtgttgtgt atcaaaaact
     2401 gggttaaatt tcggatctgc ttcaaaaatc ttggttttat ttcctgagcg aacaagcgcc
     2461 atatgacctc caactattct ttttttgcac agtagcactg tgcttttgtg cattttatat
     2521 cattatggtt ttttgcacaa gtgcacagtg ctattgtgca tttctggcgt ttgttgtact
     2581 atggtggtgg taaaaggtga tctagaactt gccaagttgc ggcaacattt gaggtaaaag
     2641 aaaaatgcag caaaatcatg atagtgatga tgattgccgc attcagttcc gagacgattg
     2701 gctataccag ttttataaat acggtaaagc caatcgaaaa attccagcta cgattagtgt
     2761 ttcattaggt cgcaagctgg atatgctgaa tgctgctgat aaaattggag atttacggtc
     2821 tcccccgggg aaccgatttg aaaatctcaa ccctccattg acagggtatt catctataag
     2881 ggtgaatgat aagtaccgct tgatatttaa gtggggtgag aacggagtcg aggatttgta
     2941 tctcgatcca catgattacc gataggtaac agaccggaac tgttatctta caaaacaagt
     3001 aggtgaggca tttcctcacc tacgttaaaa cgtcatcaca aataatgatt aataaagtaa
     3061 cgcgacgtaa ctatatttaa gataattact aatcgataca gctggtaaca gaagaaatac
     3121 ttatcttcct catctaacag agagaagcga ccaattcagg agtaaagcat gtcccaagca
     3181 accagaaaac catcgactgt aggagatatt attctttatg aatatctcga gccaagtggt
     3241 ttaaaaatca atgacttggc tgaaatgtta aacgtgcata gaaattctgc aagtgctttg
     3301 gttaacaata ataggaaact gtctaccgat atggcgtttc ggttagctaa agcattcgat
     3361 actaccgcta agttctggtt aaatatacag cagtctgttg atttgtggga ggttacgagc
     3421 agcccacgcc ttcaggaaga aatagaaaga gtcactcccg ctcatgaaat tatagaaagg
     3481 aagcacaatg ctgaatcttt ggaaaaaaaa catgcggtcg catgattttt ttaaaaaaat
     3541 ttaaccaaac tagaagaagg cctctgtaat agtgtgcctt ctttttttgt aaccgctaaa
     3601 ggtcatcatc attttctttt acgagcactt gagatccggt ttcggtacgc gtaatctctt
     3661 gctctgtaaa cggaaaaacc gccttgcagg gcggtttttc cgaaggttca aacacttgca
     3721 ggtgattgaa ccaaggtaac tggcttggag gagcgcagtc accaaaactt gttctttcag
     3781 tttagcctta acaggcgcat gacttcaaga ctaactcctc taaatcagtt accagtggct
     3841 gctgccagtg gcgcttttgc atgtcttacc gggttggact caagacgata gttaccggat
     3901 aagacgcagc agtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact
     3961 gccttcccgg aactgagtgt ctggcgtgga atgagataaa cgcggccata acagcggaat
     4021 gacaccggta aaccgaatgg caggaacagg agagcgcacg agggagccat cagggggaaa
     4081 cgcctgatat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcaatttct
     4141 atgatgcttg tcaggggggc ggagcctatg gaaaaacggc cgctaagcgg cctttccttc
     4201 tgcccatcac gcgtccgctc ggttttctcg agcatttata cgcatagtcc taccgctcgc
     4261 cgcagccgaa cggcaggagc gtagcgactg agtgagcgag gaagcggaaa agagactggt
     4321 tttacactga gcactgacgc tctgaggctt cttatgacga aacaacagaa aacagcgctg
     4381 aacatggcta aattcatcca gaatcagtct ctgctcctgc tggagaagct taacgagctt
     4441 gatcttgatg cagaggccga cctttgtgag aaactccacg atgatgcgga gcatcttttc
     4501 cgtacactgt
//
