LOCUS       NZ_CP024498             4064 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain KSB1_7E isolate KSB1_7E plasmid
            unnamed2, complete sequence.
ACCESSION   NZ_CP024498
VERSION     NZ_CP024498.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357328
            Assembly: GCF_002752905.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 12-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB1_7E
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:15:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,288
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 5,079
            CDSs (with protein)               :: 5,079
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 41 of 91
            Pseudo Genes (incomplete)         :: 49 of 91
            Pseudo Genes (internal stop)      :: 17 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024498.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KSB1_7E"
                     /isolate="KSB1_7E"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="Australia"
                     /collection_date="2013-09"
     gene            join(3855..4064,1..69)
                     /gene="traD"
                     /locus_tag="CTI55_RS27470"
     CDS             join(3855..4064,1..69)
                     /gene="traD"
                     /locus_tag="CTI55_RS27470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_099745350.1"
                     /translation="MGSKKTLEQRMALAQDRLSKLKKEASQKERKAREHKKFTVGGEVT
                     RVFGLDYDLALVVGFLEHFNKLSDEQKQALKNNGERLLKSRLENADK"
                     /name="traD"
     gene            202..468
                     /locus_tag="CTI55_RS27475"
     CDS             202..468
                     /locus_tag="CTI55_RS27475"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_113519627.1"
                     /translation="MSQKKEQKTAEKPTAGPLRKHRSRAEKIDDLKKQISELRKAEREE
                     RQRARDEKLIILAKAVYEKAGKPKDIEALIDFVKSEDFKIEYK"
                     /name="hypothetical protein"
     gene            complement(483..752)
                     /locus_tag="CTI55_RS27255"
                     /old_locus_tag="CTI55_27035"
     CDS             complement(483..752)
                     /locus_tag="CTI55_RS27255"
                     /old_locus_tag="CTI55_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_099745351.1"
                     /translation="MKGNEIRKGLTQKEMAKKFNLSLSTVKKYTSQPREHYESEAENRR
                     KTAYTLHHKKGLKWREVAEKMNTTENAVKALAKRYKQIDQENRG"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(749..1606)
                     /locus_tag="CTI55_RS27260"
                     /old_locus_tag="CTI55_27040"
     CDS             complement(749..1606)
                     /locus_tag="CTI55_RS27260"
                     /old_locus_tag="CTI55_27040"
                     /inference="COORDINATES: protein motif:HMM:NF015076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_099745352.1"
                     /translation="MILQKEYSRSDFINQIISRPFCTDDFKRDGIYRTNKRRALNSVYI
                     EHNNDSFINSIVFDIDSDTAAIAWQDADIPKPNFITQNPANGHAHLFYALSSPVCITEN
                     ARRKPQKLLKGVIEGLTERLGADPCYTGKITKNPLNPRWRTFWNEQPRFELNYLCEFID
                     TNKRVKKEVRKSIVAEGRNTALFDSLRFYAYSIIFKYQKNDDFCGFISALQEEAENIND
                     SFEDQLSFKEINHTIRSISSWVWGTFDSDTFSEIQSNRAKKAKGKTKTNTKAKKLIEFI
                     GVKK"
                     /name="replication initiation protein"
     gene            complement(1915..3174)
                     /gene="mobQ"
                     /locus_tag="CTI55_RS27265"
                     /old_locus_tag="CTI55_27045"
     CDS             complement(1915..3174)
                     /gene="mobQ"
                     /locus_tag="CTI55_RS27265"
                     /old_locus_tag="CTI55_27045"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_099745348.1"
                     /translation="MALYRLEMQNVSRSDGVSSVAKAAYRHRSVMIDERTGEIHGEKSA
                     NRDDLVYAEILAPEGTPDFLIKSSNDLWNYVEKTEKRKDARTAKEFKITLPCELTNEQN
                     IKLLKEYLTKNFVSKGIICDFVIHNDKDNKNPHAHVMITTREITLSGFGKKVRSWDEEK
                     TLHDWRKQWAKVQNQHLKNAGLKSRVSHRTLEEQKNIMIDLAKKAEDAKDFDKSILYLA
                     KAIELDRPPMQNLSRKKWRTKEGQEQRKRDQAIRDRARADARAFRLLRGVAVVDVASFV
                     VHPLEKKEQSKNNDFSEKKSWWRSTVNFIKNIKAKFSTKKDIKNDVHASDDLMTDPVTG
                     LPITKAKWQEMSDRANVRTPQPAISYQVKQSDGEGLKTAQNALQDVLDVSEGKSLKVKS
                     VPVPGSRKRRVVEEDSSDNG"
                     /name="mobQ"
     gene            3434..3865
                     /locus_tag="CTI55_RS27270"
                     /old_locus_tag="CTI55_27050"
     CDS             3434..3865
                     /locus_tag="CTI55_RS27270"
                     /old_locus_tag="CTI55_27050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157769352.1"
                     /translation="MNKKQALWIGGKMNKKEIEKRLLIFTSEIDIEDIKNDFINDEVNE
                     DVFNRCLKLYYAIDDAIYNNKKSLFEALIYLINKDRENKDHDEPIITDYDLVYLTLNNY
                     INIYELKKLFLLQKNINDFDFKSMISEIEKIKREVEYGK"
                     /name="hypothetical protein"
     Typing          complement(1919..3084)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caaaaacaag cgttaaaaaa caatggtgaa agattgctaa aatcacgtct agaaaacgct
       61 gacaagtgac agatttctaa tctgacactt ggaagggttg aacaagcaag aacccgccgc
      121 tggcggggct tgtgcgttag tcttcaccgt agcccggctt tgtgatatgt ttaaaaaaca
      181 tatcatccgg gagagtaaaa aatgtctcaa aaaaaagagc aaaaaacagc agaaaaacca
      241 actgcagggc cacttcgtaa acacagatct agagctgaaa aaatagacga tttaaaaaag
      301 cagattagcg aactaagaaa agcagaaaga gaagaacgac aacgagcaag agacgaaaaa
      361 ttaattattc ttgcaaaagc tgtttatgaa aaggcaggaa aaccaaaaga tattgaagca
      421 ttaattgatt tcgtaaagtc tgaagatttc aagattgaat ataaataaag ctaaggggca
      481 ttttagcccc tgttttcttg gtcgatttgt ttgtatcttt tggctaatgc tttaacggca
      541 ttttctgttg tattcatttt ttcagctact tcacgccatt ttaagccttt tttatggtgt
      601 aacgtataag ctgtttttct tctgttttct gcttctgatt cataatgttc tcttggttga
      661 gaagtgtatt tttttacagt gcttaaagaa agattgaact ttttagccat ctctttttgt
      721 gttaagccct ttcttatctc gttgcctttc atttttttac tcctataaat tctataagtt
      781 tttttgcttt tgtattggtt ttagttttac ctttcgcttt ctttgctctg ttgctttgta
      841 tttcgctgaa agtatcacta tcaaaagtcc cccataccca agatgaaatg cttcttattg
      901 tgtggtttat ttctttaaag cttagttgat cttcgaatga atcgtttatg ttttcagctt
      961 cttcttgtaa tgctgaaatg aaaccgcaga aatcatcatt tttttgatat ttaaaaatta
     1021 tgctgtaagc gtaaaaacgc agactatcaa ataacgctgt attccttccc tctgcaacta
     1081 ttgattttct tacttctttt tttactcttt tatttgtatc tatgaactca catagatagt
     1141 tcaattcaaa tcttggttgt tcgttccaaa atgtacgcca tctcggattg agagggtttt
     1201 ttgttatctt gcccgtataa caaggatctg cacctaatct ttcggtgagt ccttctataa
     1261 cgccttttaa taacttttgt ggctttcttc tggcgttttc tgttatacaa acaggagatg
     1321 ataaggcata aaataaatga gcgtgaccat tagccggatt ttgagttata aagttaggct
     1381 tcggtatatc tgcatcttgc catgcaatag ccgctgtatc gctgtcaata tcaaacacaa
     1441 tagagttaat gaagctatcg ttgttatgct cgatataaac gctgtttaaa gctcgtcttt
     1501 tgtttgttct ataaatacca tctcttttga agtcgtcagt gcaaaatggt cttgatataa
     1561 tttgatttat aaaatcactt ctgctatatt ctttttgtag tatcattttt taatacaatc
     1621 cttttattgt agatccctga ttttctcctg tcgccaaaca ttcggaaatc agggattttt
     1681 gtttttatat tactgttcta acataacctt tcatggtgtc aagtagtcac cactaaaaaa
     1741 gtgtcaagtg gtcattttag ccatatcagg taataatggg tctgggggta tttttgcgaa
     1801 gcacaggccg aaggcttcaa atcatcacaa gttaagctat agcctgatag gtacaggccg
     1861 taggctatga gcgaagccct gtaatcttat gtaggatctg gctgtgctgg ttagctaacc
     1921 gttgtcactg ctgtcttcct cgacaactcg acgcttacga gaacccggaa ccggcacaga
     1981 cttcaccttt aacgatttac cctctgatac gtctaaaacg tcctgtaacg cattctgagc
     2041 cgtttttagc ccttccccgt ccgattgctt aacctgatat gagatcgcag gctgtggagt
     2101 ccttacgttt gccctgtcac tcatctcttg ccatttcgct ttggtgattg gtaaacccgt
     2161 caccggatca gtcattaaat cgtctgatgc atggacatcg tttttaatat cttttttagt
     2221 tgagaactta gctttaatat tttttataaa attaacagta gaacgccacc aagatttttt
     2281 ttctgaaaag tcattatttt ttgactgctc ttttttctct aacggatgaa ccacgaatga
     2341 agcaacatca acaactgcaa cacctctcaa caacctgaaa gccctagcgt cggctctcgc
     2401 cctgtctctg atagcttggt cacgtttgcg ttgttcttga ccttcttttg ttctccattt
     2461 tttacgtgat aagttctgca ttggtgggcg atctagttca atagcttttg ctagatataa
     2521 aatagacttg tcaaaatctt ttgcatcttc tgctttttta gctaaatcga tcattatatt
     2581 tttttgttct tctagtgttc tatgactgac gcgagatttt aagcctgcat ttttcaaatg
     2641 ctgattttga acttttgccc attgtttacg ccaatcatga agggtttttt cttcatccca
     2701 tgaccttact tttttaccaa atcctgaaag cgttatttct cttgtcgtaa tcatcacatg
     2761 agcgtgcggg tttttattgt ctttgtcgtt atgtataaca aagtcgcaaa tgatgccttt
     2821 actaacaaag ttttttgtta agtattcttt caatagtttt atattttgtt cattggtaag
     2881 ttcacaaggc aaagttattt taaattcttt tgctgttctt gcgtcctttc tcttctctgt
     2941 tttttcaaca tagttccata gatcattgct tgatttaatc aaaaaatctg gcgttccttc
     3001 tggtgccaat atttcagcat aaaccagatc gtcacgatta gctgatttct cgccgtgtat
     3061 ttctccggtt cgttcatcta tcatgacgga acgatgacga taagcagctt ttgcaactga
     3121 gctgacacca tccgaacgac taacgttctg catttctaat ctatataaag ccatataatt
     3181 atatcctcat gagaacccct attttttctt ttgggtttgg gttttagttt ttcgtaagaa
     3241 aaacaggttt tagccgtcgg cagaccccgg cattgtttac aatgcgtaac cgggcattcc
     3301 ttagctttta tttttcgcta acgctcaaac taaaagagga atgaaaaaca aactagcttt
     3361 tctttcactg atatatataa attaactctg attaaattaa agtcaaattt gacaatattt
     3421 aaaagttacg atattgaata aaaaacaagc attatggatt ggtggtaaaa tgaataaaaa
     3481 agaaatagaa aaaaggctat tgatttttac aagcgagatc gacattgaag atattaaaaa
     3541 cgatttcatc aatgacgaag ttaatgaaga tgtttttaat aggtgtttaa agctgtatta
     3601 cgctatcgat gatgcaatct ataacaataa aaaatcattg tttgaagcgt tgatatattt
     3661 gataaataaa gatcgtgaaa ataaagatca tgatgaacca attataactg attatgatct
     3721 tgtgtattta acactaaata attacataaa tatctatgaa ctgaaaaagt tgtttttatt
     3781 acaaaaaaat atcaatgatt ttgattttaa aagtatgatt tcagagatag aaaaaattaa
     3841 aagagaggtt gagtatggga agtaaaaaaa cgttagagca aagaatggca cttgcacaag
     3901 atagattatc taaacttaaa aaagaagcat cgcagaaaga aagaaaagct agagaacata
     3961 aaaaatttac tgttggcggt gaagttacaa gggtgtttgg tttagattac gatctagctc
     4021 ttgtagtggg ttttcttgaa cactttaaca agttaagcga tgag
//
