LOCUS       NZ_CP024494             3223 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF249 isolate INF249 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP024494
VERSION     NZ_CP024494.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357223
            Assembly: GCF_002752775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: INF249
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:11:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,576
            CDSs (total)                      :: 5,454
            Genes (coding)                    :: 5,308
            CDSs (with protein)               :: 5,308
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 59 of 146
            Pseudo Genes (incomplete)         :: 84 of 146
            Pseudo Genes (internal stop)      :: 30 of 146
            Pseudo Genes (multiple problems)  :: 24 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024494.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF249"
                     /isolate="INF249"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Australia"
                     /collection_date="2013-12"
     gene            21..236
                     /locus_tag="CTI54_RS29130"
                     /old_locus_tag="CTI54_28615"
     CDS             21..236
                     /locus_tag="CTI54_RS29130"
                     /old_locus_tag="CTI54_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            404..736
                     /locus_tag="CTI54_RS28810"
                     /old_locus_tag="CTI54_28620"
     CDS             404..736
                     /locus_tag="CTI54_RS28810"
                     /old_locus_tag="CTI54_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            1754..2077
                     /locus_tag="CTI54_RS28830"
                     /old_locus_tag="CTI54_28640"
     CDS             1754..2077
                     /locus_tag="CTI54_RS28830"
                     /old_locus_tag="CTI54_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2286..>2720
                     /locus_tag="CTI54_RS29980"
                     /pseudo=""
     CDS             2286..>2720
                     /locus_tag="CTI54_RS29980"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     ARG             405..736
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          1467..1658
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(1004..1484)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          2068..3221
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.247"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tactggcggg gaaatagcac atgacggagc tggaaaaaca gttgctgagc gcattagagc
       61 agctacagca ggactactcg aaaaggctgg acgagtggga gaacgccttc gcggaatggc
      121 ggacgatgtc tggtcttatt caacgggaga acgcggcgct gaacgagcgc gtcacggtct
      181 tgagcaggca ggtgcagagt ttgagcgagc agctgcgccg gttgtcgaaa ggctgaacgc
      241 gatagaaact caccggcagc aggagcgggc ggtgcagcac cagaaagcgc tggagctgga
      301 acgatcgcag tggcagcatc atggtccttc gctatgataa gccgggttct attaattagt
      361 tgttatgtaa agatatttaa atttttcaac atgaggtatt tttatgaata catatatttt
      421 cctgggaggg gctattgtgg ctgaagtcat aggcaccacc tttatgaaat attcagaagg
      481 atttacacgc ctgtggccgt ctgtaggaac tgcaataagt tattgcgctg ccttttatct
      541 gctttcccaa acattggaaa atatacctac cggtattgct tacgcaattt ggtctggtgc
      601 gggaattgta ctaataagcc tagctggttg gttggttagc ggtcaaaaac ttgattggcc
      661 cgcaattgca ggaatgttcc ttatttgcgt tggagttttg gttattaata ttttttcaaa
      721 atcttctgcg cattaacttt ttaccgaagc atattggcca aagcaaaagg atttccttga
      781 gatcctttct ttctgcgcgt aatctttttc cctgtaaaca aaaaaaccac cgctaccaac
      841 ggtggttcgt ttgccggaat tcagagctac caactctttg ttccgatggt aactggcttt
      901 cacggagcgc agatacctaa actgtccttt cagtgtagcc gcactcaggc tttcacttca
      961 agaactctgt aggcattctc tacaatctct gctccgtgaa cccagttacc aatggctacc
     1021 gccactggcg atttgtcgtg tctttccggg ttggactcaa gttgatagtt accggaacgg
     1081 gcgtggtagt cggactgaac ggggggttcg tgcatacagt ccatcctgga gcgaactgcc
     1141 tacccggaac cgagtttcag gcgtggaatg agaaaccgcg gccataacag cggaatgaca
     1201 ccggtaaacc gaaaggcagg aatgcggggg agcacgaggg agccaccagg gggaaacgcc
     1261 tggtatcttt aagccgcatc gggtttcgcc accgctgatt tgagcgtcat tttcgtgata
     1321 ctcgtcaggg gggcggagcc tatggaaaaa cggcaactcc gcatcctttt cttggccttt
     1381 cctgcttcga gcggcatttg cttatcccgt aatctgtgag ctggtaacgc ccgccgcagt
     1441 ctcacggcag gagcgtagcg accgagtgag cgaggaggcg tcatttcatc accacctgca
     1501 ttttgcactt acgcccggca gccgcatttt tcccctgtca catgaagcac tttcataaat
     1561 tgccactccg tcataacatg tttagccagt atacactccg ctagcgctac gtgactggtt
     1621 cagggctgcg ccccgaaacc cgctaaaacc actgccgcgc ctgcggcttg tccaacaccg
     1681 tgccgaccgg gaaaagattt cccgtccgtc ccggcgttat caggaggccg gattggcaga
     1741 caaacgcagc aaaatgctca ccatgtgggt gactgaggac gagcaccggc gtctgctgga
     1801 gcgctgcgag ggtaagcaac tcgcggcctg gatgcggcag acgtgcctgg acgagaagcc
     1861 cgcacgcgcc ggcaaacttc cgtcgatctc gccggcgctg cttcgtcagc ttgccggcat
     1921 ggggaacaac cttaaccaga ttgcccgcca ggttaacgcc ggtggtggaa gcggacacga
     1981 ccgcgtgcag attgtcgccg cgctgatggc catcgatgcc ggactcgagc ggttgcggca
     2041 tgccgtactg gaaaagggtg ctgatgatga tcgttaagtt tcatcctcgg gggcgcggcg
     2101 gcggcggcgg gccggtcgat tatctgctgg gtaaagatcg ccagcgcgac ggcgccagcg
     2161 ttctgcaggg gaagccggat gaagtccggg agcttatcga cgcctcgccc tacgccaaaa
     2221 agtacacttc cggcgttctg tcttttgccg aacaggattt accgcccggc cagcgcgaaa
     2281 agctgatggc gagtttcgag cgggttctga tgcccggact cgataaagac cagtacagcg
     2341 tgctgtgggt tgagcaccgg gacaaggggc ggctggagct gaacttcctg atcccgaaca
     2401 cggagcttct gaccggcaag cggctacagc cgtattacga ccgcgccgac cgtccgcgca
     2461 tcgatgcctg gcagaccata gtgaacggca ggctggggct gcatgacccg aacgcgccgg
     2521 agaaccggcg ggtgctggtc tcgccttccg cgctgccgga agcgaagcag gaagccgccc
     2581 aggcgattac gagcggcctg cttgcccttg cctcatccgg ggagctgaaa acgcgtcagg
     2641 acgtcactga ggcgctggaa agcgcaggtt ttgaggtggt gcgcaccaca aaaagcagca
     2701 tcagcattgc cgacccggac ggggggcgaa acatccgact gaagggagcc atctatgaac
     2761 agtcttttaa cgctggcgaa ggacttagag cagaaatcga aagcgcagca gcagactacc
     2821 ggcgagatgc tgaaagccgc attcagcgag cacgagaagt ctgtcagagc ggaactgagc
     2881 gaaagcgaga agagaatcag cgccgccatc ctcgaccacg accggaagct gtcctcagcc
     2941 atgagccagc gcacgaaagg gatgctgcgc atggtcagcc agacgtggct gaccatcgtt
     3001 ctggtctccg cgctgctgat agcgtcgagc gcgggcattc tgtggtggca ggggcagcag
     3061 atactcgaga actatacgac catccgggag cagaagagca cgcaggccat gctgtcagag
     3121 aggaacagcg gcgtacagct ctcgacctgc ggcgagcaga gacgccgctg cgtgagggtg
     3181 aacccggaag cgggacagtt cggagaggac tcgagctgga tga
//
