LOCUS       NZ_CP024493             3514 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF249 isolate INF249 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP024493
VERSION     NZ_CP024493.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357223
            Assembly: GCF_002752775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: INF249
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:11:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,576
            CDSs (total)                      :: 5,454
            Genes (coding)                    :: 5,308
            CDSs (with protein)               :: 5,308
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 59 of 146
            Pseudo Genes (incomplete)         :: 84 of 146
            Pseudo Genes (internal stop)      :: 30 of 146
            Pseudo Genes (multiple problems)  :: 24 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024493.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF249"
                     /isolate="INF249"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Australia"
                     /collection_date="2013-12"
     gene            complement(join(3065..3514,1..48))
                     /locus_tag="CTI54_RS29110"
                     /old_locus_tag="CTI54_28580"
     CDS             complement(join(3065..3514,1..48))
                     /locus_tag="CTI54_RS29110"
                     /old_locus_tag="CTI54_28580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(726..1067)
                     /locus_tag="CTI54_RS28785"
                     /old_locus_tag="CTI54_28590"
     CDS             complement(726..1067)
                     /locus_tag="CTI54_RS28785"
                     /old_locus_tag="CTI54_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2238..2429
                     /locus_tag="CTI54_RS28800"
                     /old_locus_tag="CTI54_28605"
     CDS             2238..2429
                     /locus_tag="CTI54_RS28800"
                     /old_locus_tag="CTI54_28605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071008427.1"
                     /translation="MLKKNEIILIKSCGNSKTKIFLRGVDEGLMIDASFEDIKFAINNN
                     LHDLLGIQAETTKLKLTK"
                     /name="hypothetical protein"
     gene            complement(2843..3058)
                     /locus_tag="CTI54_RS29120"
                     /old_locus_tag="CTI54_28610"
     CDS             complement(2843..3058)
                     /locus_tag="CTI54_RS29120"
                     /old_locus_tag="CTI54_28610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1153..1325)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.655"
                     /coverage="100.0"
                     /evalue="9.54e-56"
     Typing          1250..1796
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(3..736)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="4.67e-164"
     Typing          complement(733..1046)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="9.860000000000001e-59"
     Typing          complement(2728..3512)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.53e-167"
ORIGIN
        1 tttcgatttc tcctcgaagt ccctcgaggc cgttaaaaga ctgctcataa atggctcccc
       61 tcagtcggat atttcgcccc ccgtccgggt cggcaatgct gatactgttt tttgtggttc
      121 tgaccacctc aaaaccggcc gctgtgagcg cccctgtgac gtcatcacgc gttttcagct
      181 ctcctgatga ggctaacatc agcaaacccc gtgtaatcgt ctctgcggct tcctgcttcg
      241 tttctggcag tcctgaaggc gtgaccagcg cccgccggtt ctccgggtcg ttcggatcat
      301 gtagccccag cctgccgttg accactgtct gccacgcatc gatgcgcggc cggtcggcac
      361 gatcgtaata cggctggagg cgtttccccg tcagtaattc ggtattgggg atcagaaaat
      421 tcagctcaag acgacccttg tctgcgtgtt ccacccacag aatgctgtac tggtctttat
      481 cgagtccggg catcagaacc cgctcaaagc tggccatcag tttttcacgc tgacccggcg
      541 gcaggtccgc ctccgcgaat gacagcaccc cggaggtata ttttttcaca tacggcgacg
      601 catcaatcag ctcccggact tcttccggct tcccctgcag gacgctggca ccttcacgct
      661 gccggtcttt acccagaaga taatccacag gaccggcgcc cccgccgcga ccccggggat
      721 ggaatttaac aatcatcgtc cggccctttt tccagtacgg catgccgcaa ccgttccaga
      781 ccggcatcga tcgccatcag cgctgccacg atctgcaccc ggtcatgacc ggcaccaccg
      841 gcgttcactt tccgggcgat ctggttcagg ttattgccca tgccggcaag ctgacgcagc
      901 agcgcgggcg aaatcgaggg aagcctgccg gcgcgtgtcg gtttctcatc aaggcaggtc
      961 tggcgcatcc acgccgccag ttgttttccg tcgcaccgct caagcaaccg ccggtgttcg
     1021 tcttccgtca cccacatcgt gagcatcttg ttgcgtttat ctgccagaat cgcctcctga
     1081 taacgccgtg acggacggga aagcctttcc cggtcggcgc ggggttggac aagccgcagg
     1141 cgcgtcagtg gggtttcggg gcgcagccct gaaccagtca cgtagcgcta gcggagtgta
     1201 tactggctta gtatgttggc atgagtgcga taatcaggaa agcgccttat gcggcagaag
     1261 aaaaatgctg cagcagagcg taagcagaat atgcgataca gaagatattc cgcttcctcg
     1321 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtgccggc ttacgggcgg
     1381 ggcggaaatt tccaggaaga taccaggaag aaacttaaca gggaagcgat gaggccgcgg
     1441 cgaagccgtt tttccatagg ctccgccccc ctgacaagca tcacaaaatc tgacgctcaa
     1501 atcagtggcg gcgaaacccg acaggactat aaagatccca ggcgtttccc cctggtagct
     1561 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     1621 cgcgtttatc tcattccacg cctgacactc agttccggga aggcagttcg ctccaagctg
     1681 gactgtatgc acgaaccccc cgttcagtcc gactgctgcg ccttatccgg taactatcgt
     1741 cttgagtcca acccggaaag acacgccaaa gcgccactgg cagcagccac tggtaacagg
     1801 attagcagag cgagttaatg taggtgatgc tacagagttc ttgaagtggt ggcctaacta
     1861 cggctacact ggaaggacag tatttggtat ctgcgctctg ctgaagccag ttacctggtt
     1921 aagcagttcc ccaactgact taaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt
     1981 tacagagcaa gagattacga cgaggctgaa tgaatctcaa gatttcattt agcctgccac
     2041 ccgatcattc aaagttttgg cgtagttctg acccgctccc cgtgatttgt tacataccca
     2101 ccacagggca ttagtttttt tatttttttg atgatatgta caaaatcagt ggttaggaat
     2161 aatcttagca gccttaacaa acacacacaa aaacaacgat aatgattcat ctgacgacaa
     2221 caaacaatga caatgtcatg ttaaaaaaaa atgaaattat cttaataaaa tcatgtggta
     2281 actcaaaaac aaaaattttt ttacgaggtg tggatgaggg actcatgatt gatgcaagtt
     2341 ttgaagatat aaaatttgct atcaacaata acctccacga tcttctcgga atccaagccg
     2401 aaaccacaaa actaaaacta acaaagtgat acagcccctg aagcatgaag gatcgaaata
     2461 aagccaccga tttaccataa cgtcttctta cccgcaccgc gccggaggca ctcagggcgt
     2521 ttctgatggc tgacgggcaa aaggtggtgg caagtacacc gaaaaccacc agaaacgcac
     2581 ctgatttatt ttacgcatat gcatcactta ttcacatcac tgcatagccg gtgatttcgg
     2641 ttaaaaaaga ctctctgtgt gctgaaatcg cagaaaatgg ccatgatccc cgtcacgctc
     2701 aggccacctg agacgggcgt ttatcagtct ccatactccc tgtcaccatg agataatgtg
     2761 cgttgccgtt ccggtaccag atctgctgtc ttctctggtt ccttttccgt tgtttgcccg
     2821 tatcgctgtt gctcaatctc attcagcctg tcgataaccg gcgcagctgc tcgctcaaac
     2881 tctgcacctg cctgctcaag cccgacacgc gctcgctcag cgccgcgttc tcccgttgca
     2941 taagaccaga catcttccgc cattcctcga aggcgctctc ccactcgtcc agccttctcg
     3001 agtagtcctg ctgtagctgc tctaacgcgc tcagcaattg tttttccagt tccgtcattc
     3061 tgctctactc ccgtaccagc ttcaccgcgt tccgcgtctt gttgtccacc gtccggcccg
     3121 tttcggcttt catcccctcc ggcagcacca gaaaccgtcc gttgcggttc tccagatagg
     3181 tcacgcccca cgtctgcatg tgcagtttcg acagcgtatc tctctgctgc ctgatttccg
     3241 ccaggttcgc cgctatccgg ttcccctggt accacagcac cccgctcagg ctggcgaaca
     3301 gcagccctga caccatggtg atggtcagcc acgtccgccc gaccattcgc agcacgctca
     3361 gccggttcga ctgcatcgcc gcgttcatcc cctgttcgtg ggcgctgatg gcgtcgttga
     3421 ttctcttcgc gcttgcgttc agttccgatt tgacaacgtt ctcgtgcttg ctgaatgcgg
     3481 ctttcagcct ctcttcggta agctgctgct gtgc
//
