LOCUS       NZ_CP024491             6657 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Klebsiella pneumoniae strain INF249 isolate INF249 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP024491
VERSION     NZ_CP024491.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3357223
            Assembly: GCF_002752775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlic,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: INF249
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 11:11:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,576
            CDSs (total)                      :: 5,454
            Genes (coding)                    :: 5,308
            CDSs (with protein)               :: 5,308
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 59 of 146
            Pseudo Genes (incomplete)         :: 84 of 146
            Pseudo Genes (internal stop)      :: 30 of 146
            Pseudo Genes (multiple problems)  :: 24 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024491.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF249"
                     /isolate="INF249"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="Australia"
                     /collection_date="2013-12"
     gene            322..513
                     /locus_tag="CTI54_RS28685"
                     /old_locus_tag="CTI54_28495"
     CDS             322..513
                     /locus_tag="CTI54_RS28685"
                     /old_locus_tag="CTI54_28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            513..917
                     /locus_tag="CTI54_RS28690"
                     /old_locus_tag="CTI54_28500"
     CDS             513..917
                     /locus_tag="CTI54_RS28690"
                     /old_locus_tag="CTI54_28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_099755384.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLETSE
                     LLRVFGAFAPVSVEQAEEQVSGQRDTVTSDRNEQVIHTLKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREEKRQNTTRPRAWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(924..1622)
                     /locus_tag="CTI54_RS28695"
                     /old_locus_tag="CTI54_28505"
     CDS             complement(924..1622)
                     /locus_tag="CTI54_RS28695"
                     /old_locus_tag="CTI54_28505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071080961.1"
                     /translation="MTRTFILLCTGVFAIAYALYELKHMGVYYPDASNPQLFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRSSESRRIQREISGYQQALREAEEAHTARQNALI
                     RDHNMKTETLHSHYCDAANVLREEYAKREKRWRQALQNAREEARQELEEQTEALNQRAS
                     GLLVQEQTLKARMEEVRKYVAGERHNAMRARLEAEEAEKRRQNASATAERRRRKLEKLQ
                     ATDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(1619..2506)
                     /locus_tag="CTI54_RS28700"
                     /old_locus_tag="CTI54_28510"
     CDS             complement(1619..2506)
                     /locus_tag="CTI54_RS28700"
                     /old_locus_tag="CTI54_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="WP_099755385.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKE
                     NRLNHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAADP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLIPSPLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="integrase"
     gene            complement(2499..2720)
                     /locus_tag="CTI54_RS28705"
                     /old_locus_tag="CTI54_28515"
     CDS             complement(2499..2720)
                     /locus_tag="CTI54_RS28705"
                     /old_locus_tag="CTI54_28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            complement(4088..4408)
                     /locus_tag="CTI54_RS28710"
                     /old_locus_tag="CTI54_28520"
     CDS             complement(4088..4408)
                     /locus_tag="CTI54_RS28710"
                     /old_locus_tag="CTI54_28520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202963179.1"
                     /translation="MMRVLSLGAILMAMSALPLTAISQVTLAPDGSYVSGSEATLAPDG
                     SYVGGSEVTLAPDGSYVGGSEVTLAPDGSYVGGSEVTLAPDGSYVGGSEATLAPDGSYV
                     GN"
                     /name="hypothetical protein"
     gene            complement(4540..4731)
                     /locus_tag="CTI54_RS28715"
                     /old_locus_tag="CTI54_28525"
     CDS             complement(4540..4731)
                     /locus_tag="CTI54_RS28715"
                     /old_locus_tag="CTI54_28525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049106033.1"
                     /translation="MLKKDDIIFMKSCGNAKTIIFLRGVDEKLMINASFEEIKFAINNN
                     LQELPGVQAVAATLKIAT"
                     /name="hypothetical protein"
     gene            4973..6083
                     /locus_tag="CTI54_RS28720"
                     /old_locus_tag="CTI54_28530"
                     /pseudo=""
     CDS             4973..6083
                     /locus_tag="CTI54_RS28720"
                     /old_locus_tag="CTI54_28530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     Typing          complement(2..274)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.331"
                     /coverage="92.0"
                     /evalue="1.87e-77"
     Typing          complement(6455..6657)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.549"
                     /coverage="87.0"
                     /evalue="2.13e-93"
ORIGIN
        1 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc accaggggga aacgcctggt
       61 atctttatag tcctgtcggg tttcgccacc gctgatttga gcgtcagatt ctgtgatgct
      121 tgtcaggggg gcggagccta tggaaaaacg gcaacgccgt ggcctttgcc acaggctctt
      181 ctcttcctgg tgatagcgat tactcttctg tgtgtcccgt ccgctcgccg cagccgaacg
      241 gcaggagcgt agcgactgag tgagcgagga agcggaaaag agaccggttt gacactgagc
      301 actgacgccc tgaggcccct tatgacgaaa caacagaaaa ctgccctgaa catggctaaa
      361 ttcatccaga atcagtccct gctcctgctg gagaagctta acgagcttga tctcgatgcg
      421 gaggctgacc tttgtgagaa actccacgat gatgcggagc atcttttccg tacactgtcc
      481 ctcagactgg atgcactaca ggagggtgac tgatggcgaa agtgtcgatc agtgaggctg
      541 cacgcctgac gggtaagagc aggacaaccc tgcacaggtt aattaaagcc ggtgaactgt
      601 ccacctgtcg gggtgaacgg aatgcgaaaa tgctcgagac ttcagagctt ttgcgggtgt
      661 ttggtgcctt tgcgccggta tccgttgaac aggcggaaga acaggtcagt ggacagcgtg
      721 acacagtcac ttctgaccgt aatgaacagg tcattcatac tctgaaacag gaaattgaac
      781 acctgaacac ccttctggcc tcaaaagatt cacatatcga cagtctgaaa caggcgatgt
      841 tgttgctgga gaaccgggaa gagaagcggc agaacacgac ccggccccgt gcctggtggc
      901 gtttctggca gtcctgaaat gcctcagtgt ttttgccctg ctgttccgtc agtggcctgg
      961 agtttttcca gtttccgtct gcgccgttcc gctgttgcgc tggcattttg tcgccttttc
     1021 tctgcttcct ctgcctccag acgggcccgc atggcgttat gccgctctcc ggcaacatac
     1081 tttctgactt cctccattct ggctttcagt gtctgctcct gtaccagcag gccggacgcg
     1141 cgctgattca gtgcctccgt ctgttcctcc agttcctgcc tggcttcttc ccgggcattc
     1201 tgcaacgcct gacgccagcg cttttctctt ttcgcatact cttcccgcag tacattcgcc
     1261 gcatcacaat agtgactgtg cagggtttct gtcttcatgt tgtgatccct gataagggca
     1321 ttttgtctgg cggtatgcgc ctcttctgcc tccctcagtg cctgctgata tccgcttatc
     1381 tcgcgctgta tccggcggga ctctgagctt ctgaaaaaca aaatgaacag ccacagcgag
     1441 aacgctgtgg ctgcgatccc cgtagccagg attgcgggac cgagttcggt gaccgccttc
     1501 cagccagtga acagctgcgg attactggca tccggatagt aaacgcccat atgtttcagc
     1561 tcatacaggg cgtacgcgat ggcgaaaaca ccggtacaca ataaaataaa cgttcttgtc
     1621 atcgcaggta tgtctccgtg atttccggac gaaaatggcc catttcctgg ctgacggtgc
     1681 gcagggccat atcacgaacc agaccgagtt tttgcagctc ctgcatccgt tgctgggcgt
     1741 agctgtgacg cacgccgtgt gccccccgtg accagcccag ggtacgcatg gaggcggcac
     1801 tgaaagcgtt cgaccagcgc tggcctccgc caatggcgta ctgactctgg taaaacacgc
     1861 cgcgatcggt cacccggacc gggctcgcca gcctgacatg ctccagacgt tcagccaggg
     1921 gggacggtat caggacttcc ctgaccaggc cgcctttgcc ctgaacggtg taaatcacac
     1981 cctcccgacc ggcaaattta ctgtccgctg ccggacgggg atccgctgct cgctcctggc
     2041 ggggcgcgag ggtcagcagt tcatgggcgc gtaatccggc ggccagggca atcgcggtgg
     2101 ccagagcgtt ggcgggctgt tgtgctgcgg caatcagggc tacctggtcc gggctgtagg
     2161 cccggctgct caggatctgt gcctgctccg atttcaccac cggcaggtgt tcgccggcag
     2221 ggagggtctg cgtcacgtgg atcatcatgg cctggatggc ctggcgttcc atatcgagcg
     2281 ttttctggcc gacctgctcc ccgcgctgct ccaggtaggt gatggcctgc gccggtgtca
     2341 gtgtgcgtaa atcaccgtgg ttgagccggt tctctttcat ccactctgcg acgcgggtca
     2401 gtccctgctc gtaattccgg actgtgccga cagagcgcag aatatttccc tggagtggtt
     2461 ttatcacgcc agccgcctgg cgggcagcgc tggcgaattt agccattttt ttccagccag
     2521 cgggtaaccg tggacaacac cactttgatt ctccgggccc ggagccagcg ttgcagctcc
     2581 gctgcactcg ccccggcctg gtacaggctc agcagttcgc cggtatagcg gtcgaggcgg
     2641 gagggacggt agcggacctg gcggatacgg cgggtctgcg cctgcagggc ggccagttcg
     2701 gcagcggtat caaattccat ctgtactccg gtgtgtaaga acatcagtgg aaatccactg
     2761 cggcactggc cgattcgccc tcgtttaaac cgtatcgtgg ttcagcccgg actgctcgcg
     2821 gctgtagtaa aaatgacgtc cctttagcgt gggaggcgcg tggcccgttt gtgggcctga
     2881 cagatttggg ctgttttctc agagttctcg gattgggctg cccggtacag tatccccgga
     2941 gggacccggc tgtttaacct gctgccggta ggcaggtgcg cgtcagagtg cgcagtctca
     3001 tggtttgact aaatactagc caggtttttc gtgcgtgtga atgttgtttt ttaaatgttg
     3061 cgtctgactc cgatcaatag cgactcccgc tatgtacagg cgcattttat gcgtcgtaca
     3121 tagcgggagt cgcaggtgct gatttggcgg aataaaaaac accgggactg accgccccgg
     3181 accccgccaa cggtgaccgg acagacaaaa aatttgcctg cccggtcacc gtttcacatc
     3241 atggcgagga ggcgggatct gaaacggtgt gatgaaagtg cagatgaggt cagacgatgc
     3301 aggtcggtga gtccgtggtg aacgcgatag tggaatacca cgcacagggc agtgaggagg
     3361 cgcatgcaga ggaaaacagg atgttcctgc gttatctgcg cgagttaacg ggaacacagc
     3421 gtgtggcggt tgaggtggcc aggaaatatt tttctccggt gaggggctga tatgagctac
     3481 ctttttcttg tcgccggtac cgcaccaggt gcgacggtcc ttttacagcg ttgtcctcat
     3541 ttgacgggcc tggatggccc gttcttttat gcttttaacg tccggggccc ttttctcggc
     3601 cgaaatcacc tactatgcgg tggtgtgcat agccatgcat gtgcgtaaaa taaatcaggt
     3661 gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt accggtttcc cgtcagaaac
     3721 gccctgaggc cgttttcgcg gtgcgcgtaa gaagacgtta tgttaaatag ggcgctatcg
     3781 cctaaatgca cgactgattg attttttttc tctgaagtat gagattcctg agtagacagc
     3841 tacaacaagt agagcaatgg cgatagcaag taaaacaaag tccataagta cctgattaat
     3901 ttaataaaaa acctgttatg attagttatt gtaagtggtt ttcattcttt tttcattaca
     3961 cttattgctt tgagctaatg gttcctgagg acgcattgtt cagtacacgt aagaagacgt
     4021 tatgataaat ccaaaaatgc tggcattcgg aattcagtat atcggtacgc agcataataa
     4081 tggggggtta atttccaacg tatgagccat caggtgctaa ggttgcttcc gaaccaccaa
     4141 cgtatgagcc atcaggtgct aaggttactt ccgaaccacc aacgtatgag ccatcaggtg
     4201 ctaaggttac ttccgaacca ccaacgtatg agccatcagg tgctaaggtt acttccgaac
     4261 caccaacgta tgagccatca ggtgctaagg ttgcttccga accactaacg tacgatccat
     4321 caggtgctaa ggttacttgg gatatcgcgg ttagtggtaa tgctgacata gccattaaaa
     4381 tggcaccaag agataatact ctcatcacta ttactcctgt taatatcaaa aatagaattg
     4441 aatcgttcag cggtgccgaa cgccggttcc agccgcggga ttcgtgatcg ggatgttata
     4501 gtaaattgat ctcttgattt tgagtgccct tactcgaaat caggttgcta ttttcagtgt
     4561 cgcggctact gcttgtaccc ccggaagctc ctgaagatta ttattgatag caaactttat
     4621 ctcttcaaag ctcgcgttaa tcatgagctt ctcatccacg ccacgtaaaa atattattgt
     4681 ttttgcatta ccacatgatt tcataaagat aatatcatct ttttttagca tgatactgtc
     4741 gttacttagt gtagtgagat aaagcatagc cactgttttt gtgtgttttt gttaaggctg
     4801 ttaagattat tcctaaccac tgattttgta catatcatca aaaaaaataa aaaaactaat
     4861 gcccccgtgg tgggtatata acaaatcacg gggagcggag ctgacctgct ccccgttgat
     4921 taatacaccg cgatgttagt aatgtcttca taagccacat gaggacatcc tcatgaagaa
     4981 gcgtttttcc gacgaacaga tcatcagtat tctccgcgag gccgaagccg gggtttctgc
     5041 ccgtgagctc tgccgtaagc acgccatttc cgacgccatc ttttacacct ggcgtaagaa
     5101 gtatggcggt atggaggtgc ccgaggttaa gcgccttaag tcgcttgagg aagagaacgc
     5161 cagactcaag aaactgcttg ccgaagccat gctggataag caggcgcttc aggtggctct
     5221 ggggcgaaag tactgacgac agaccagaag caggaagccg ttgagtttat gtgtgatgcg
     5281 accggtctgt cgcaacgtag tgcctgcagg cttacaggtt tgcccctgtc gacctgccgc
     5341 tatgaggctc agcgttcggc ggctgatgcg catttatcac tgagctggca ctggagcgca
     5401 ggcgttttgg ctaccgacgc atctggcagt tactgcgccg tgaaggcctt catgttaatc
     5461 acaagcgcgt gtaccgtctt taccacctta acgggctggg tgtaaaacgc agacgacgtc
     5521 gtaaaggtct ggcaacagaa cgtctgccgc tgctccgccc ggaggcgccc aacctgacct
     5581 ggccgatgga ttttgtcatg gacgcgctgg ccaccggtcg caggatcaag tgcctgacct
     5641 gcgtggacga cttcacgaag gagtgtctga caattaccgc cgtcttcggg atttcaggcg
     5701 ttcaggtcac gcgaattctg gacagcattg cactgtttcg cggctatccg gcgacgataa
     5761 gaactgacca ggggccggag tttacctgca gagcactcga ccagtgggct tatgagcatg
     5821 gggtggagct acggcttatc cagccgggca ggccaacaca gaacggattt attgaaagtt
     5881 ttaacggacg attcagggat gagtgcctca atgagcactg gttcagcgat atcgttcacg
     5941 ccaggaaaac gattaatgac tggcggcagg attagagcga gtgtcgtcca cattcatcgc
     6001 tgaactacca gactccggct gaatttgcgg cgggctggcg aaacgggaaa tatgaagaaa
     6061 aacgaaccga cattactaac tgaaggttgt atctaatcct ggggggcagg tcaaccgcgt
     6121 tggatgattg tggatcatga ttatatatgt tttttttctt atcagaaaca atgggttaaa
     6181 tagttttatt ttgatggttg gtgtcgcgga gtggtgctgg tttcacttca ggggtaggca
     6241 tatggaacag gatcctcttg agatcctttc tttagcatcg taatctcttg ctcagtaaac
     6301 gaaaaaaccg ccctgcaagg cggttttttc gaaggttctc tgagctacca actctttgaa
     6361 ccgaggtaac tggcttggag gagcacagtc accaaaactg ttcgtttagt ttagccttaa
     6421 ccggcgcata acttcaagtc catctcctct aaatcagtta ccagtggctg ctgccagtgg
     6481 cgcttttgca tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagca
     6541 gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga
     6601 actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccgg
//
