LOCUS       NZ_CP024465             1551 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain QS17-0161 plasmid pMRSN480738_1.6,
            complete sequence.
ACCESSION   NZ_CP024465
VERSION     NZ_CP024465.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07792166
            Assembly: GCF_002752955.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Snesrud,E.
  TITLE     Sequencing of mcr-1 carrying Klebsiella pneumoniae from Thailand
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Snesrud,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2017) Multidrug Resistant Organism Repository and
            Surveillance Network, Walter Reed Army Institute of Research, 503
            Robert Grant Ave., Silver Spring, MD 20910, United States of America
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 03:00:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,574
            CDSs (total)                      :: 5,451
            Genes (coding)                    :: 5,315
            CDSs (with protein)               :: 5,315
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 65 of 136
            Pseudo Genes (incomplete)         :: 78 of 136
            Pseudo Genes (internal stop)      :: 37 of 136
            Pseudo Genes (multiple problems)  :: 33 of 136
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024465.1.
            Bacteria and source DNA available from The Multidrug Resistant
            Organism Repository and Surveillance Network (MRSN).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="QS17-0161"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /culture_collection="MRSN<USA>:480738"
                     /db_xref="taxon:573"
                     /plasmid="pMRSN480738_1.6"
                     /geo_loc_name="Thailand: Chonburi"
                     /lat_lon="12.6890 N 100.9820 E"
                     /collection_date="2017-05"
                     /collected_by="AFRIMS"
     gene            complement(734..1210)
                     /locus_tag="CR231_RS28855"
                     /old_locus_tag="CR231_28700"
     CDS             complement(734..1210)
                     /locus_tag="CR231_RS28855"
                     /old_locus_tag="CR231_28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(50..141)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.398"
                     /coverage="100.0"
                     /evalue="3.4000000000000004e-27"
     Typing          1290..1398
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.182"
                     /coverage="100.0"
                     /evalue="4.23e-47"
     Typing          243..503
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.985"
                     /coverage="100.0"
                     /evalue="1.85e-99"
ORIGIN
        1 cgttcaacag ctcgatcatg tttttgcctt ccgttcaccg ctaaaacgcc ccaaaaatcg
       61 ggacaggatg tgcaaatgta ataccgtcac ccgcgacgat attccgtttg ccatctggtc
      121 agggcttcgc cccgacaccc cgcatcagtg cgtacgtgct caatgtggtt tccggttctc
      181 ttccgcttcc tcgcccactc gctcactacg ttcggtcgct ggggctgcgg cgagcgggat
      241 cacctctcgc atgttgtcgg tgatcccggt gttgttcttt tggggatgag tcgccagata
      301 gcccctcaaa cgcgctcaaa agctctcaga cgcacgaaac gggttttaga tgcccgccag
      361 ggcatggaag gcgcgaaaaa cgcttacaga ggctctcagg aggtcaagcg cgccgatacc
      421 cctaaccagg ggctttcagg tcgccaggaa ccgcgccagc cccctgcaaa gcccatttgc
      481 acggaacagg cagccatgcg gttggtaggt ggtcgtagac cgtcggtgtt gcagcgatgg
      541 gggtctgggg ttctccccag acggtttccg gtagcgctca aggcgctgcc ggaaccgtgg
      601 cggctcggtt ttttctggag aacggggagc aaaaacacgc aaaggatttt gaggcggcaa
      661 aagagcaatg caggatagac aatcatggtc agaacgttaa cacgtagccc ctgattgtcc
      721 gcctgccgct agctcaattt gaaaacatgg ccatgattcg gcgctgaata tcaccgtctt
      781 tgttgaccag tgttttagtg acaaacgctg catggttttt cgtagtgttg tatcccttcc
      841 agacttcatg tggatcgagg gcatacgcac aaaccttacc agcaacgggg attctaacta
      901 atgcattttt tgattctaag taattgagcc accgaataat tgtgcttctg ctaactccaa
      961 gtttttcagc taggaattgc tgatccgcaa cgacagcgcc acacgtagga tcgatatgct
     1021 ccgctaaaaa tgagtacagc ttcagtgctg atatgccttg gctatccttc agcagctctc
     1081 gaacacgttc ccaacctttt ggggatacct gtgtaaacct tgaatttttc tttgcttctt
     1141 cgagatcctc ggtgtttttc tttgcctcat ctgaaagttg tttcagtcgt ttttctgaat
     1201 caataaacat gattttttcc ctctgaaaat cccatgcact aacgttggtt caaatctaag
     1261 ccatcatgac acatctgtgc aacaccttgt tgcgtgttgt cgcatccagg atgcgacaac
     1321 gttgtgtcat cctggatgcg acaggtaaat aaaaaacaca ctaaaaaaac aagcacttac
     1381 aaatcggcgt aaaagatcta ttctaggcaa gggtgttgcg tgcaacgcaa cgccctttgg
     1441 gggtgtcggg gcgaagccct gacccagtca cgtagcgata gcggagtgta tactggctta
     1501 ctcctctgca agcctcgtca gccgtgattt tgcacgattt cgcgctctcc a
//
