LOCUS       NZ_CP024464             2780 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain QS17-0161 plasmid pMRSN480738_2.8,
            complete sequence.
ACCESSION   NZ_CP024464
VERSION     NZ_CP024464.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07792166
            Assembly: GCF_002752955.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Snesrud,E.
  TITLE     Sequencing of mcr-1 carrying Klebsiella pneumoniae from Thailand
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Snesrud,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2017) Multidrug Resistant Organism Repository and
            Surveillance Network, Walter Reed Army Institute of Research, 503
            Robert Grant Ave., Silver Spring, MD 20910, United States of America
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 03:00:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,574
            CDSs (total)                      :: 5,451
            Genes (coding)                    :: 5,315
            CDSs (with protein)               :: 5,315
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 65 of 136
            Pseudo Genes (incomplete)         :: 78 of 136
            Pseudo Genes (internal stop)      :: 37 of 136
            Pseudo Genes (multiple problems)  :: 33 of 136
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024464.1.
            Bacteria and source DNA available from The Multidrug Resistant
            Organism Repository and Surveillance Network (MRSN).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="QS17-0161"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /culture_collection="MRSN<USA>:480738"
                     /db_xref="taxon:573"
                     /plasmid="pMRSN480738_2.8"
                     /geo_loc_name="Thailand: Chonburi"
                     /lat_lon="12.6890 N 100.9820 E"
                     /collection_date="2017-05"
                     /collected_by="AFRIMS"
     gene            complement(23..424)
                     /locus_tag="CR231_RS28820"
                     /old_locus_tag="CR231_28665"
     CDS             complement(23..424)
                     /locus_tag="CR231_RS28820"
                     /old_locus_tag="CR231_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032446877.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLSSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(424..615)
                     /locus_tag="CR231_RS28825"
                     /old_locus_tag="CR231_28670"
     CDS             complement(424..615)
                     /locus_tag="CR231_RS28825"
                     /old_locus_tag="CR231_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032446876.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNEFDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1029..1112
                     /locus_tag="CR231_RS29930"
                     /old_locus_tag="CR231_28680"
     CDS             1029..1112
                     /locus_tag="CR231_RS29930"
                     /old_locus_tag="CR231_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072042462.1"
                     /translation="MHEPPVQSDCCASSGNYRLESNPERHD"
                     /name="replication initiation protein"
     gene            complement(1361..2041)
                     /locus_tag="CR231_RS28840"
                     /old_locus_tag="CR231_28685"
     CDS             complement(1361..2041)
                     /locus_tag="CR231_RS28840"
                     /old_locus_tag="CR231_28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072042463.1"
                     /translation="MGRKSREKKERHIKDRASPLKVYRFFKEKSHAEALCNGDVWLSTI
                     ETCRRYEDPLQGDPNEAIQTYSSGFISGGSDDSRFVSAAAHAGIHIGPGCSGITIQNCR
                     SFNKMPDAFVLCTTLEFQPENLNDTFGNYCVEISNVEEFFGLITKKLKGLLPIKLGAMG
                     EVRYGSHSFYGYQPPPGPIGFVKNRDKYSSQKEFRFLWVPETFDIQPRLVKCPELSYCC
                     RMIS"
                     /name="hypothetical protein"
     Typing          664..1142
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.501"
                     /coverage="92.0"
                     /evalue="2.14e-161"
ORIGIN
        1 ggaaaactca agcatggcag gatcaggatt ttttccagaa tttccaccat ggcgccgggg
       61 attcagttaa tttggcctgg tgattttcaa gaaggagcat ggcctgtttc aggctgtcaa
      121 tgtgcgaatc cttggacgaa agtaaggtgt tcaggtgttc aatctcctgt ttcagatgac
      181 gaatcacctg ttcactctgg tcagcagagg ctgtatcacg ctgtccagag acctgtccag
      241 aatggtgttc acccttcggt tgctcaaagg gaccaaagac acgtaaaagc tctgaagtat
      301 ctaccatttt tgcattgcgc tcaccgctac aggtggacag gtcacctgct ttaattaacc
      361 tgtgcagtgt tgttctactc ttacccgtca gtcgggcagc ctcactaata gacactctgg
      421 ccatcagtta ccatcctgta atgcatccag tctcgaggac agtgtacgga aaagatgctc
      481 cgcatcatcg tggagtttct cacaaaggtc ggcctcgaca tcgagatcaa actcgttaag
      541 cttctccagc aggagcagag actgattctg gatgaattta gccatgttca gcgctgtttt
      601 ctgttgtttc gtcataaatg gcctcagagc gtcagtgctc agtgtaaaac cagtctcttt
      661 tccgcttcct cgctcactca gtcgctacgc tccggtcgtt cggctgcggc gagcggcagg
      721 agcatgcgta taaaaactcg agaagaccga gcggtcaaat ggggaagaag gaaaggccgc
      781 aaagcggccg ttttttcata ggctccgccc ccctgacaag catcacggaa tctgacgctc
      841 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggtgg
      901 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
      961 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     1021 tggactgtat gcacgaaccc cccgttcagt ccgactgctg cgcctcgtcc ggtaactatc
     1081 gtcttgagtc caacccggaa agacacgact aatcgccatt ggcagcagcc actggtaatt
     1141 gatttagagg agttagtctt gaagttatgc gccggttaag gctaaactga aaggacaagt
     1201 tttggttact gcgctcctcc aagccagtta ccgcggttca aagagttgct gctctaagaa
     1261 ccttcaaaag gaccgccttg ctgggcggtc tttttgtttc caaaggagat tacgctggta
     1321 acgtaatctc agtcaagaat tacactatgg aaaatgtcat ctaagatatc attcggcaac
     1381 aatatgaaag ctcagggcat ttgactaacc tgggttgaat atcaaatgtt tctggcaccc
     1441 aaagaaatct gaattctttc tgcgaagaat atttatctcg attttttaca aacccaatcg
     1501 gtcccggtgg aggctggtaa ccataaaaag aatgactccc atatctgacc tcccccatag
     1561 cacctaattt tataggtaat aaccccttta actttttagt aataagacca aaaaactctt
     1621 caacatttga tatttccaca cagtaatttc caaaggtatc gttcaagttt tctggctgaa
     1681 attccagtgt cgtacataaa acaaaggcgt cgggcatctt attaaaagag cgacaatttt
     1741 gtatcgttat tccactacag ccaggtccaa tatggattcc ggcatgagct gcagcagaga
     1801 caaaacggct gtcatcactg ccccctgaga taaaacccga gctataggtt tgaattgctt
     1861 cattcggatc tccctgtagg gggtcttcgt atcgtctaca cgtttcaata gtgctcagcc
     1921 aaacatcgcc attgcacaat gcctcagcat gactcttctc tttaaaaaac ctatatactt
     1981 taagggggct ggctctatct ttaatatgcc tttctttttt ctctctactt ttcctaccca
     2041 ttgcaaaata tcctttttaa attttcattt accataacat ctcttgcccg caccgctaaa
     2101 acggcctcag ggcgtttctg acgggaaacc ggtaaaacag gcaacaaacc acccgaaaac
     2161 ccgccagaaa cgcgcaggct ctgtttttac ggcatgcatg agtatgcacg caagtgcgta
     2221 gcgcgaaaat tcacgccatt cagccatggt aagtcctgtt tccggcgttc ttcccatccg
     2281 aaaacggtga acggggacgc aatttattgc gtggccgtga agcgttgccg gcgcagccgg
     2341 aaaaccggtt ttcagcacgt tcaggaatcg tgttcgtcga cggccggcaa tcacgttatg
     2401 gcaagtcgcc ggacgactca ccacacgatt ccccggcgac tcttcactag agtcgtaccc
     2461 agagtcgccg agtctaaaaa acaaccaaac ggggagtcgt gtctagagtc gggtcgttat
     2521 taactcattg tttttatagt cctgagtcgt taccgactct atgacgactc gccggggact
     2581 ctatgacgag tcgtcataga gtcggatcta tgtaaataaa atttaaaaaa cgaaatttca
     2641 ggaaagtaaa ccactgatat taaatacaaa tctgcaaaat tttaattttg cgtagtgtgt
     2701 gggtattttt agtcgtgagg agcgttcaga gatctggcga tttttgggaa ggttcaggaa
     2761 ctccccggga tggccggaga
//
