LOCUS       NZ_CP024463            10046 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain QS17-0161 plasmid pMRSN480738_10.0,
            complete sequence.
ACCESSION   NZ_CP024463
VERSION     NZ_CP024463.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07792166
            Assembly: GCF_002752955.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Snesrud,E.
  TITLE     Sequencing of mcr-1 carrying Klebsiella pneumoniae from Thailand
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   Snesrud,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2017) Multidrug Resistant Organism Repository and
            Surveillance Network, Walter Reed Army Institute of Research, 503
            Robert Grant Ave., Silver Spring, MD 20910, United States of America
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 03:00:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,574
            CDSs (total)                      :: 5,451
            Genes (coding)                    :: 5,315
            CDSs (with protein)               :: 5,315
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 65 of 136
            Pseudo Genes (incomplete)         :: 78 of 136
            Pseudo Genes (internal stop)      :: 37 of 136
            Pseudo Genes (multiple problems)  :: 33 of 136
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024463.1.
            Bacteria and source DNA available from The Multidrug Resistant
            Organism Repository and Surveillance Network (MRSN).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="QS17-0161"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /culture_collection="MRSN<USA>:480738"
                     /db_xref="taxon:573"
                     /plasmid="pMRSN480738_10.0"
                     /geo_loc_name="Thailand: Chonburi"
                     /lat_lon="12.6890 N 100.9820 E"
                     /collection_date="2017-05"
                     /collected_by="AFRIMS"
     gene            complement(join(9808..10046,1..19))
                     /locus_tag="CR231_RS28755"
                     /old_locus_tag="CR231_28600"
     CDS             complement(join(9808..10046,1..19))
                     /locus_tag="CR231_RS28755"
                     /old_locus_tag="CR231_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_047067361.1"
                     /translation="MGLKLNLTWFDKKTEEFIGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGGFDVEKSWVPLLQPYFKNKIEVDKYWYQISFDYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(29..1717)
                     /locus_tag="CR231_RS28760"
                     /old_locus_tag="CR231_28605"
     CDS             complement(29..1717)
                     /locus_tag="CR231_RS28760"
                     /old_locus_tag="CR231_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_099722058.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVNVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGVKDYGHDYHPAPKTEEIKGLGELKKAPKKTPKQGGGGRRDRWIGDKGRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQIKGPDPKGRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(2302..2820)
                     /locus_tag="CR231_RS28770"
                     /old_locus_tag="CR231_28615"
     CDS             complement(2302..2820)
                     /locus_tag="CR231_RS28770"
                     /old_locus_tag="CR231_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020956924.1"
                     /translation="MQKVTDLYPPEIARHKLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVAHSLKQKYLPVTARTISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKRTNAQKEMSRLYKEFGEDGILALLRNVAQSNGT
                     PPPGAHPAS"
                     /name="hypothetical protein"
     gene            3594..3857
                     /locus_tag="CR231_RS28775"
                     /old_locus_tag="CR231_28620"
     CDS             3594..3857
                     /locus_tag="CR231_RS28775"
                     /old_locus_tag="CR231_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_099752041.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPESDPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGITTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            3847..4146
                     /locus_tag="CR231_RS28780"
                     /old_locus_tag="CR231_28625"
     CDS             3847..4146
                     /locus_tag="CR231_RS28780"
                     /old_locus_tag="CR231_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023389908.1"
                     /translation="MPYDVEWKQGAIEDVTMLFDYIAENSSLWDAQNVTDRVLTAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4208..4585)
                     /locus_tag="CR231_RS28785"
                     /old_locus_tag="CR231_28630"
     CDS             complement(4208..4585)
                     /locus_tag="CR231_RS28785"
                     /old_locus_tag="CR231_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004146445.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTPRRWWQWGKR"
                     /name="hypothetical protein"
     gene            4654..4833
                     /locus_tag="CR231_RS28790"
                     /old_locus_tag="CR231_28635"
     CDS             4654..4833
                     /locus_tag="CR231_RS28790"
                     /old_locus_tag="CR231_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4861..5391)
                     /locus_tag="CR231_RS28795"
                     /old_locus_tag="CR231_28640"
     CDS             complement(4861..5391)
                     /locus_tag="CR231_RS28795"
                     /old_locus_tag="CR231_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004146446.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRTQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKSARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5398..6129)
                     /gene="mobC"
                     /locus_tag="CR231_RS28800"
                     /old_locus_tag="CR231_28645"
     CDS             complement(5398..6129)
                     /gene="mobC"
                     /locus_tag="CR231_RS28800"
                     /old_locus_tag="CR231_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_020956927.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQISVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(6129..8090)
                     /locus_tag="CR231_RS28805"
                     /old_locus_tag="CR231_28650"
     CDS             complement(6129..8090)
                     /locus_tag="CR231_RS28805"
                     /old_locus_tag="CR231_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_099752043.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRVRRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGITWCAGGLV
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(9574..9723)
                     /locus_tag="CR231_RS28815"
                     /old_locus_tag="CR231_28660"
     CDS             complement(9574..9723)
                     /locus_tag="CR231_RS28815"
                     /old_locus_tag="CR231_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_020956931.1"
                     /translation="MKKVKVVFLFILIFLGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..6717
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          8893..9471
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5402..6129)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.062"
                     /coverage="100.0"
                     /evalue="9.930000000000001e-144"
ORIGIN
        1 aatttaattt aagccccata cttacctctc aaagatattt tttaatgttt cgtcctttcg
       61 gatccggacc tttaatttgc ttacccgttt ttggatcaaa tgccccgagg tgttcgccat
      121 cactggcacg atacccttca agctcaccgt gctgggagtc ccattcataa atcttacggc
      181 ctttatcacc aatccaacgg tcacgtctac caccaccacc ctgtttaggt gtttttttag
      241 gtgctttttt taattccccc aaccccttga tttcttcggt cttcggagcg ggatgataat
      301 catgaccgta atctttaact cccttgcgag gcttgttctt ttcctcattt aacttatttt
      361 ctgcatcctt cgcttttttc tccttttgtt tccggctctc catcgccccg gtaagtgcag
      421 ccttatctct ggataaggat tcattcattg cgtttaaaga tgcctgagcc gcttttaatt
      481 tctcattctg attctgaata tccttatcaa gatagcgaac ctgatcctgc cacccggagc
      541 catattcatg aggcgggtta taagtcacaa aatcatcttt atttgcctct gcctctttta
      601 cctttttatc ggcttcctga acagcagccc ttgcaccggg gagcgccttc tctgtcgatg
      661 caatgcggct gtttaaggtc gcaatatttt tatcttcggc atccagctca gctttcgctt
      721 tatcatagtc tcttttcagc ccctcttccg gatgagcggc gtcccatgcc tgctggcgac
      781 gcttttcttc ctcctgacgc tgttttacct gtgccggggt aagaacatct gtcaccgaca
      841 cataaaccgg cttctgtccg gtctctttcg ggaaacgaat aacggcctca cgggagttac
      901 caccggctgt aaaaccagcc ggacgtgaat cacctttttc agcaacaatg ccttttggcg
      961 gggctttcgc tgtcggaaca cctttcggta cgttcacctg cagagacggg agacccggaa
     1021 tcgacacact gaataccccc ggacgttttg tcggtttcgc atccaccaca gggacactca
     1081 ttggccgacc tgcgacaacc gcaatatgct gccgctcatc cttcaccaca tccacaaccc
     1141 gttgtctgac acgaaccgtc gcctggtcca gtggtaaagt acttgccgga gtctccgtca
     1201 ccgcatcggc cggtaatgac gtcataattt ttgacatcat tttcggatca tcttttgcaa
     1261 tctcagaagg cagcacaccg tagatcgcaa tcccccacag accaaactta aacggcccct
     1321 tcagggcagc cagcacatca gcaacggcgg ccgacaacgc atcgccggaa actgataaag
     1381 ccagtccttc agcccccgga gtagccaggg caggcagacc gaaggccatc gcagtggccg
     1441 aagactgtcc gccaccagag ctaccgctat gattgccgct gttaccacca ttaccaccgg
     1501 gtttactccc gccgttacca aactcccccg gattatagtt atgaatatct ccattcggcg
     1561 tatgcgtcgt accccagccc ggcccgctgt ttggatccgt accaccacct gatgaagggc
     1621 cacctttacc agacgtaccg ttcacattcc cactggcctg accaccatta tgtcccagac
     1681 ctgaattacc cggacctcga ccgtctccac cactcatatc gttttcctct ttgacattta
     1741 aaacaataaa ttaaaaccaa tatactgtat atatacacag tataattgtt accttactcc
     1801 tccagttttc aggtgtcaat atatgagatt agttctcatc ttcaggcaca aaaaagcgcc
     1861 cggagccgcc tgtaagcaga tcgcgtcttt tcccgaaaat ttctcatggc aacaaatttt
     1921 attatttgag acattcatca cacttacttt tatattggta tagatgtacc tgaaagcgaa
     1981 atggcatgag gagagtacct gcggttaaac tgttcaggaa tcggagacaa cccggaggcc
     2041 accatgacgc acctgaagaa aagcgcctgg cggacgcctt tgtcgctcag gcactggcca
     2101 tgggcctgaa aggggttcga ggtccaaccg tacgaaaatg tacggtaaga gtgaaattct
     2161 cgtccgaaag aaggaaaagc aaaactggta acgccaccgc cggtagaagg gaactatctg
     2221 caccgcgatt agctgacaga tcaagagctg gcggcatcca gaaaacctta aaaataaagc
     2281 cccttagggg ctttactggt atcaacttgc agggtgtgcg cctggcggcg gtgtaccgtt
     2341 cgactgggca acattgcgta acagcgccag tatgccgtct tcaccaaact ccttgtacaa
     2401 ccggctcatt tccttctgag cattggtgcg cttctggctg ttcaggttac gttcgcactc
     2461 tttcatcatc ttttccggct ctgatttcag ccttgcaaga tcgtcctcat cgatacagaa
     2521 agccgcctgc tttaccgcac cacctacatc agcctttgtc gaaatcgttc tggccgtaac
     2581 tggcagatat ttttgcttaa gcgaatgggc cacggctgac gcacggttaa cacatagatc
     2641 ggccgtgatg ttatagccgg aagtggtcac tacgttgccg tcacagagcg ccgcaaaagt
     2701 acacaggctg gttttattta gtttctggat taactccagc ataacggtat taccggcgaa
     2761 gtgattctgg agcttatggc gagcaatttc aggaggatac agatcagtta ctttttgcat
     2821 ttatagcacc tgtttacaga ggataaataa caggtgacgg tgtccggatg agcattcgca
     2881 catcaaaaaa ctgtgaaaaa tgcctggcaa gtcggtacca agttactaca taatgcagca
     2941 ccacttgaca aacgccctaa tcgggcgttt gttgatcagg taagattcag ttttcacgaa
     3001 ctgagtcacc ggcagctatc agaatcctta gtttctcagg ccgaagatct gatagctgcc
     3061 tcgtttttca ctgacaacct cacgatcatc agaatccctt attctacctt attccataag
     3121 cgcacacata gcttttttgc tgatctttcg cgcttgattt acaaggaaat tacctgtcgg
     3181 gtcgatgaaa ttagcaacag cgctatcaga tgacgctaat gttaaactca tgtttaaaca
     3241 tacagcgaaa agttgacgca aaaatcgtca gctgaatgcc tcataaccag ccatttacca
     3301 taaaacccgt tatcagcacc gcccgagagg ccctcagagc gtttccggcg gctacgggat
     3361 aaaacaggca acaaatcacc cggaaaaccg ccggaaacgc gcccggtaaa ctttatccta
     3421 atgcatgcct atgcacgcaa ctgcatagca tatgttttga cgtaaaatgg atgccgtaag
     3481 cccttttttt accgactttc tgcttcatca gcctgactga cacgaccata ttctttcaga
     3541 ccgccccctg ctaaacttct tcatatcacc gcattaatcc gggagcctcc gcaatgagca
     3601 acactacaca ctacgaaaac gccaacttcc tgcgggaact ggctgaaaac ctgccccgca
     3661 tactgccgga aagtgacccg gacaaagctg cgttgttgca acgcctggct aacgaagaac
     3721 tggcccaggc agagtatgaa gatcaggttc gcgccaaagt aaccgccgcc cgtgctgaca
     3781 cgcgtccggg cataaccacg gaacaactcc gccagcgcct gcatggccgt tatcaggaac
     3841 tgcgcgatgc cgtatgatgt ggaatggaaa cagggagcca tcgaggatgt aacgatgctg
     3901 tttgactata tcgcggaaaa ttcatccctg tgggatgcgc agaacgttac cgatcgcgtt
     3961 ctcactgcgg cggacaaact ggccgacttc ccgcgcctct atgaagtgga ctcacgctat
     4021 ggcgaggacg ttcgccggat cagtctgatg ggacagcatg tcctgtatga tgttgatgac
     4081 cagaccagga aagtccgggt tctggccgtg gttggccagc gccagaatcc ccataccgtt
     4141 cgctgataac cggtgacggg ggcgcaattc attgcgtggc cgtcgccggt tggacaagca
     4201 cagcgcgtca gcgttttccc cactgccacc accggcgcgg tgttccggcc ggtgtctctg
     4261 ctgtctccgg tttgtgttcc agcagtctca tggcctgcat ttcctgccgc agtccggcca
     4321 cctcctccct gagtgctttt atctccgcga tcaacgcgtc ggaggccgtt ttttcagtct
     4381 catccggcag tctcaacccg tcccgctcgt gagtctcacc ctgtcttaac tcaccgtaca
     4441 cccggataag ctcgctggta tccaccaccc gaccaccatc agcttccgta cggtatgata
     4501 cgcggccttt cgccatatcc cgatacaggg aactccgggc tttccccgtc agttccgtcg
     4561 cctgtctcac agtcacccac gccattgtct cactccgcta tttttgagac agtatacccg
     4621 acaggcgcac accatgcccg gaaggaaaga atcatgacaa aacaggaaaa aactgccctc
     4681 aatatggcta aattcattaa ggaccagtcg ctgctgttgc tcgagaaact gaacgagctg
     4741 gatatggata ccccggccga catgtgcgag cggctgcacg aggatgcgga aacactctgg
     4801 caggtgatgc gggataagct cggggaggaa taacagcacc ggcatggccg gtgctggcgt
     4861 ttattttttg ccgaacggcc accaccggcg ggcggatttg tcctccggca gttcaggcac
     4921 cggcacgtta aaatagcggc tgttatattc ccgcatcagc tccgctttca gcttctcgtt
     4981 caccgcgctt tccccgtgcc gctcgctgac cacctcaatc atcgcgctaa tctgctccgg
     5041 tgagcacttc ccggcatcaa ccacctgcac accgttctcg atggcgaacc gctccacctt
     5101 gcgatagagg taatcacaca gcttcattcc tgcgggtttg tcccgttccc attgcggatt
     5161 ttcaccggtg aaaaagtacg tggctttttc gtcgctgttg agccgctcgt catacatccc
     5221 gatggatttc atcatcgcca gatgctgggt ccggtcgaca aaacagaaaa catacgcccc
     5281 ggtctgccag gcgagcacca ccatatcttc aagatgctcg ctggtcggca tgatgcagcg
     5341 ctcgccgtcc cggtcctccg catcttcaat aaataccatg tacagcgcca taatccctca
     5401 ctccgttgtc ataaatttaa agcacgcaaa cagccgttca cgatccagct ccgccgtttt
     5461 gcgggcctcg ccggtctgtg gattcacgat ttcatccacc gacaggatcg cccgctgcac
     5521 cctgacctgc gtcgcccggt ccgggcagat gtaccagatt tcatcgatgc cgttcgcccg
     5581 gcggttaaac agatacccgg acaccacctc ctgatagcgc ctgcggctct tcacggtgcg
     5641 ctccacctca aacgccaccg ttctgccgtc cagctccgcc agcgcgtccg gcaccttcat
     5701 ccctttcagg gcaatacggt gcaggtaccg ccagccggtg gccccccggg cctccatggc
     5761 gaggcgcagg gactgcacgg ccagccggtg cggcagctgc gccgccgaca cccgcgacgg
     5821 ctgaaacgga ataatgtccg tcagcggctc gtcctcgtcg aacgagaacg ccaccccgtg
     5881 cggcgtcagc ccccacacgc tgatctgacg gccgtcaacc gggtatttcg cccggaccag
     5941 cagccctttc gccaccaggc tgtccaggaa gcggtaaacc ggctgtaccg ccgcaatccc
     6001 cagccgtcgc gcaaccagct ccgccgtgga acacagctct tcccgcagcc agcgcagggt
     6061 cagccgtgtt ttctccgcgt tccgcgcctg ccgtttcgcg tggctgacgt tgtatcgctc
     6121 cagtgccatc agtacagccc cgcaaaatca tcatcacccg gccgctgccg cccggcctcc
     6181 gactggtact ccgccagcgg gtccgttttc gctagcaccg ggaaggtttt cagcggaatt
     6241 tcccggcgat caacccgcag cgggctgctg aacgccagcc gcgccacgcc cagcccggtc
     6301 atcaccgcga acccgtcagg cagatgttgc agggtattca cgtcaaacag cggccgcgcc
     6361 tccttctgga tgaagctgtc gccgctgatg tgctccacgt tccccgcctc ccggctggcc
     6421 cggcggcgct ccacgtccac ccggatctcc ccggtctggc ccgccgccca ctgcgcggac
     6481 tcctggctgg ccgcgcgata gaaccagcgg atcggcgtgt tatccagtac cgtggttttc
     6541 acaaaatcag cgggcagatc ctggccgcac tggccgaagt cgcccagcga ctggtgcgcg
     6601 agcagcaggt tgcagtcccg gctgcgcagc gtccccagcg cgttcagcac gtatttcgac
     6661 agcagatatt taatctcatc gagcatgatg ctcgcgtgcg gccacctccg gaactcatcc
     6721 cgcgcaatga tgatttgcgc gcagcgggca aacaacatct tctgcacccg gatcaccgcc
     6781 tcatcgtcca tcgagccgat gacgtacagg cagccgccgc cgttgattat ccccgccacg
     6841 tcgatgccgc tgtccgtctg caggacgctc aggtcggcca cgttctccag cttggtgatc
     6901 agccccttca cgtccttttt cagggcctcc ggcagcgcgt atgccgccgc cagcacctgc
     6961 tgaatgtttg ccccctgcgg gaactgctcc gcaatcagct tcgccgcttt ctgctcgctg
     7021 atgcggtagt ggtccgccac gtcgccggtc tcactcaggt taaacccggc aaccagcaga
     7081 ttcttcaggg cgtactggtc gagatcgcgg aacagattca gctgcgccgg tttccccggc
     7141 cgcagatcca gcagggtgaa gtccgggcag tgcgcccgga agacgtgcgg tgcccacgcg
     7201 tcccctttcg ggtcgaagta caccaccgcg tccccgaaat cgcgtacgca gcgaatgagc
     7261 gcgttggtgg ccatcacgct cttgccgctc ccgggcaggc cgaggatctg aatgttggtc
     7321 ttgcgccagg tatcacgcgg gaccagcacc ggctggccct gctcatcctt acccagaaac
     7381 accgcctgct gtttccgggc ctgctccgtg gaaaaatcgg ccagcgcgtt aaccggtacc
     7441 ggctccccga aatgcaggtg gcggacgtcg gtcatttcac cggtccgctg gctgctgcgc
     7501 cggctctcct ggttcagttt cgcgttcacc gcctcgtcag cggccgcaaa atgaccgtca
     7561 agcagcagga acgccagcag ccgcgccaac acaatcccga caaccagccc gcccgcgcac
     7621 caggtaatcc cgaacaggtt agccggctgc agcaggtaat aatctgcgtt cgcgtagagg
     7681 tccggacgca ggacggtttc tccccagctc tcgataaaca cggcgacaac aagccccccc
     7741 agaagggcca gaccggcagc gccggtcagc cagtacgcgt agttccagac atgccgccga
     7801 accctgacca tccgcccggg cgaatccagc gaggccagca ccgaaaatgg cgcagtccgg
     7861 ttattatcgc gcttatagcg atcggcatac tcccgccgct ccttatgact cagccccaca
     7921 aaacgcatcc gtccggccca gagttcagcc agaccgaaaa acaggccgat aaccagaaaa
     7981 gccccggcca gaccgtaata ctcccggtac agccagctga cccacgggta aatctgccgg
     8041 gccagctccg ggatatgcca ctgccaggca agcgaatccg tattaaacat gcaatctctc
     8101 tccttcagac gacgggttta ccggcgtgtt gtgcagcccc tgctgcacaa gccgcggaca
     8161 tgccccaagg catggcggcg gcggtcacgt ccgtggtgat tgcgttcagc tttcaccgcg
     8221 gaggccagcc tctttccgcc ccatgcggaa aaggcgcagc cccggaggaa caacgagcac
     8281 gccccagcgt gtgagaggaa aaggcagcac cccagtgcgg tcttttccgg tgttccggcc
     8341 cccgtcagag ttcgccatag cgagcggctg accacccgca aaatcaggag cagctttgct
     8401 gcttcgtcag gtgcgttcag caccgtggat ttttgtgggt ggtcaggcgt gagcctgacg
     8461 ggggccaggt gaatttctgg cgtggccggc gtccagccgg acagagccag aaagaggggg
     8521 aaagaccgtg cgtcggtggc gtccagccag cgtaagcagg ggatttcccc ctgaagtcag
     8581 ccacgcccta gcggggatga cttgcatttc tccgtaagga gaaaaaagca acagagatag
     8641 gttaacgtaa aatgttcagc gaaaaaccga catggttcac ctatcctgat aattgatcgt
     8701 cagggaatag aaagacttaa tcaggggaca atatcccaca tcagcgcgtc cggtgaccgt
     8761 cttgcaggtg ccggggaaat tgcgcctcct cgctcactcg gtcgctacgc tccgggcgtg
     8821 agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt ggataagcag
     8881 gggactaaca tgtgaggcaa aacggcaggg ccgcgccggt ggcgtttttc cataggctcc
     8941 gccctcctgc cagagttcac ataaacagac gctttttcgg tgcatctgtg ggagccgtga
     9001 ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag atccccaccg
     9061 tttccggtag gtcgctccct cttgcgctct cctgttccga ccctgccgtt taccggatac
     9121 ctgtccgcct ttctcccttg cgggaagtgt ggcgctttct catagctcac acactggtat
     9181 ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta agcaagaact ccccgttcag
     9241 cccgactgct gcgccttatc cggtaactgt tcacttgagt ccaacccgga aaagcacgca
     9301 aaacgccact ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa
     9361 cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt
     9421 tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt
     9481 cgatcaaacc acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa
     9541 aaaaaggatc tcaagaagat cctttgatct tttctactga accgcgatcc ccgtcagttc
     9601 agaggaggac gatggtgcca ccgttccacc ctgaacatcc cggatatagt ttgcctgaca
     9661 tgcgaccaga agaaagccta aaaaaatcaa tataaaaaga aaaacaacct ttaccttttt
     9721 catatacctt ccctgtattt aaaaaccccg gcattcatcc ctgaacaccg gaagtctgta
     9781 tttcaaatca tccttgataa ttttttgtta ccagttaccg tcccgataat caaatgaaat
     9841 ttgataccag tacttatcga cttcaatttt atttttaaaa tatggctgca acaacggaac
     9901 ccatgatttc tcaacatcaa acccgccatt gttaatatta tcctttaaag gcattccaag
     9961 gctttcaatg acagaaccat catcaccaaa gtctttagaa tactcctcac caataaattc
    10021 ctcagtcttc ttatcaaacc aggtta
//
