LOCUS       NZ_CP024197             3825 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae isolate KSB1_5D plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP024197
VERSION     NZ_CP024197.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06112197
            Assembly: GCF_002741685.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlick,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlick,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB1_5D
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 83x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 03:14:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,735
            CDSs (total)                      :: 5,613
            Genes (coding)                    :: 5,446
            CDSs (with protein)               :: 5,446
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 167
            CDSs (without protein)            :: 167
            Pseudo Genes (ambiguous residues) :: 0 of 167
            Pseudo Genes (frameshifted)       :: 53 of 167
            Pseudo Genes (incomplete)         :: 113 of 167
            Pseudo Genes (internal stop)      :: 16 of 167
            Pseudo Genes (multiple problems)  :: 15 of 167
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024197.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="KSB1_5D"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.8457 S 144.9821 E"
                     /collection_date="2013"
                     /collected_by="University of Melbourne"
     gene            join(3733..3825,1..180)
                     /locus_tag="CS526_RS29760"
                     /old_locus_tag="CS526_29585"
     CDS             join(3733..3825,1..180)
                     /locus_tag="CS526_RS29760"
                     /old_locus_tag="CS526_29585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019405140.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     gene            2336..2527
                     /locus_tag="CS526_RS29775"
                     /old_locus_tag="CS526_29600"
     CDS             2336..2527
                     /locus_tag="CS526_RS29775"
                     /old_locus_tag="CS526_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2527..2928
                     /locus_tag="CS526_RS29780"
                     /old_locus_tag="CS526_29605"
     CDS             2527..2928
                     /locus_tag="CS526_RS29780"
                     /old_locus_tag="CS526_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1500..1714
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.562"
                     /coverage="100.0"
                     /evalue="1.62e-49"
     Typing          complement(1003..1519)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.847"
                     /coverage="87.0"
                     /evalue="5.5e-153"
ORIGIN
        1 gatgtgacct acaccgtttt tgggtatgac cggaaaggta aaatacaggt caccacggga
       61 tgctgtgccg gaatgctgac agagccggtt ctgctgggtg tctgtgggtg ctttgacccg
      121 gaagaacgtg acgagatcat gcagaatcac cccatggcaa agcagttttt cacagagtga
      181 ttccgtatct gttgagtctg aagcccgcct tgtgcgggct ttttcatatc tgcgtgaaat
      241 gagacgttat ggtaaatctc taaaggcaat atttcccgtc ccttggagcg aactactctg
      301 tatttgatct ttggtgttga taccccgctg cttgcagagg ggggattcat tatcgttgtt
      361 gtttgctgta attgtctaaa atatatttgg ggatagatac gggtacacat tcagcagata
      421 ttatgttttt cccggatata ttatgataaa tgtctttgtt tgcaatgatt ttatctttga
      481 attcaatgca ttcttcattc agaatgaagg tgttaacaac attgtctttc attcccaaat
      541 ttgctaattc tgaagaacaa cttgcaatat tattcgtgtt aaaacaagtt gttagtatta
      601 ttgcgtatat gtatggcatt aaataaacct atttaaaaaa gtacggaatg atagtgatct
      661 tgttcggaaa tatactagtc agactttaag tctcagggat atgaacctag aacatcgttg
      721 tattaacttg tttgagtgtt ccaatcctga atattgatgt tatttgagta taaaggaaac
      781 ataataaata tcacaaggat attggttaac tatttatttt taaataattt cttgagatct
      841 gacgttggac gtcgtagtct cttgccatgt aaacgaaaaa accaccttgc agggtggttt
      901 ttcgaaggtt cagtaaaccg gcaagtttct gaaccgtggt aacagtcttg tgcgagacgt
      961 caccaaatct gtccttttag tgtagcctca gtctggccac cacttcaaga actcttcgta
     1021 aatctctcgc acatcctgtt taccagtggc cgctgccagt ggcgttaagt cgtgtctttc
     1081 cgggttggac tcaagacgat agttaccgga aaaggcgcag cggtcgggct gaacgggggg
     1141 ttcttgcata cagcccagct tggagcgaac tgtctaaacg gaacggggcg tggtgattta
     1201 ggtaaagctt ccaccactac acggatgccg caggacggga acaggagagc gcaagaggga
     1261 gccaccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt
     1321 tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggctttgc
     1381 cgcggcctta tcgtatccct gttaagtttc ttcctggcat ccaccaggaa ataaatttcc
     1441 gccccattag taagcctgca ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1501 gagcgaggaa gcggaatatc acctgaacca gcatcttctc ctgacgccgg aaagcctcat
     1561 ttcactcctg ccacatgaag cactttggca aatcaccact tcggtttaaa atatttagcc
     1621 agtatgcact ccgctagcgc tacgtgactg gttcagggct gcgccccgaa acccgctaac
     1681 acccctcagc tactggatat cacaccatgc catctgagta tgatgaacaa ccggatgcga
     1741 ctgatgctct gtttcccggc gaatatgacc tttttgactt aatggctcat gttactgaag
     1801 agaacctgca cgccccggtg gattctggcc cgcctgtcgg ccgggaagcg ttatagcttt
     1861 ttcgctggcc agcgtacatg taatacgctg gtattacatg ttatttgttt attttcatgt
     1921 tgttacagat gattgtaata cgttgcgtat tacgctaata cacgaaataa ctccattttt
     1981 ttcatttaat tcatgcagtt gcgataaaaa tcgcggctgc gttaggtgta tgaccattta
     2041 aggggcaaaa aattatcatt ctggctgggt gatctgcgca ggttaaggat catcggcgac
     2101 gaaaaagcgt ctttaactgc ttcgttcatg cttgcatgaa cggattattt ttccagacta
     2161 caggtaaact acaggaagac tacaaaagtt gatgatttaa tcctgcgttt tagtgcgatg
     2221 actacagata aactacaaaa taactacagg ataacctcag atccgttctc tgaaccattc
     2281 gtacatccga cccccttacc ggtttacact gagcactgat gctctgaggg cccttatgac
     2341 gaaacaacag aaaacagcgc tgaacatggc taaattcatc cagaatcagt ctctgctcct
     2401 gctggagaag cttaacgagc ttgatctcga tgttgaggcg gacctttgtg agaaactcca
     2461 cgatgatgcg gagcatcttt tccgtacact gtcctcgaga ctggatgcat tacaggatgg
     2521 taactgatgg ccagagtgtc tattagtgag gctgcccgac tgacgggtaa gagtagaaca
     2581 acactgcaca ggttaattaa agcaggtgac ctgtccacct gtagcggtga acgcaatgca
     2641 aaaatggtag atacttcaga gcttttacgt gtctttggtc cctttgaaca accgaagggt
     2701 gaacaccatt ctggacaggt ctctggacag cgtgatacag cctctgctga ccagagtgaa
     2761 caggtgattc gtcatctgaa acaggagatt gaacacctga acaccttact tttgtccaag
     2821 gattcgcaca ttgacagcct gaaacaggcc atgctccttc ttgaaaatca ccaggcaaaa
     2881 ttaactgaat ccccggcgcc atggtggaaa ttctggaaaa aatcctgatc ccgccatgct
     2941 tccattttgc tctccggcca ttccggggag ttcctgaacc ttcccaaaaa tcgtcagatc
     3001 cctgaacgct cctcaccact aaaaataccc acacactacg caaaattaaa attttgcaga
     3061 tagaccttta ttttcaatca gttatctgtt ttaaattacg caattttatt ttttgtaagg
     3121 tgaatccgag tcgtggacga ctctatggcg aatcgccgat gacttgccca cgattcatat
     3181 gtgaatcacg ccatataatc agtacgttat gatgacttga ctctagagtc gacttgccgg
     3241 gttggtggct ttttgaccat ggcgactcta gagtcaagtc acgagtcgaa tcgccggtga
     3301 attgtctggt gagtcgtccg gcgacttgcc ataacgtgat tgccggccgt cgacgaaccc
     3361 gattcctgat cgtgctgaaa accggttttc cggctgcgcc ggcaacgctt cacggccacg
     3421 caataaattg cgtccccgtt caccgttttc ggataggaag aacgccggaa acaggactta
     3481 ccctggctga atggcgtgaa tttccgcgct atgcacttgc gtgcatactc atgcatgccg
     3541 taaaaacaga gccagcgcgt ttctggcgga ttttcgggtg gtttgttgcc tgttttaccg
     3601 gtttcccgtc aggaacgccc tgaggccgtt ttcgcggtgc gcgtaatggg acgttatggt
     3661 aaatcaacgg cggatgcctg agtctgtgtt agagtcagcg gattgtgata atcagtgact
     3721 cagggagatg ttttggctac tgacgctcag gtaaaagaaa ttaatgcact catcaaaaaa
     3781 tacccggact catgctccat ctgtcatgaa gtctatgatg aggat
//
