LOCUS       NZ_CP024196             4510 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae isolate KSB1_5D plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP024196
VERSION     NZ_CP024196.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06112197
            Assembly: GCF_002741685.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlick,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlick,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB1_5D
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 83x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 03:14:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,735
            CDSs (total)                      :: 5,613
            Genes (coding)                    :: 5,446
            CDSs (with protein)               :: 5,446
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 167
            CDSs (without protein)            :: 167
            Pseudo Genes (ambiguous residues) :: 0 of 167
            Pseudo Genes (frameshifted)       :: 53 of 167
            Pseudo Genes (incomplete)         :: 113 of 167
            Pseudo Genes (internal stop)      :: 16 of 167
            Pseudo Genes (multiple problems)  :: 15 of 167
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024196.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="KSB1_5D"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.8457 S 144.9821 E"
                     /collection_date="2013"
                     /collected_by="University of Melbourne"
     gene            complement(join(4226..4510,1..36))
                     /locus_tag="CS526_RS29720"
                     /old_locus_tag="CS526_29580"
     CDS             complement(join(4226..4510,1..36))
                     /locus_tag="CS526_RS29720"
                     /old_locus_tag="CS526_29580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            219..461
                     /locus_tag="CS526_RS29725"
                     /old_locus_tag="CS526_29540"
     CDS             219..461
                     /locus_tag="CS526_RS29725"
                     /old_locus_tag="CS526_29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     gene            complement(1003..1449)
                     /locus_tag="CS526_RS29730"
                     /old_locus_tag="CS526_29550"
     CDS             complement(1003..1449)
                     /locus_tag="CS526_RS29730"
                     /old_locus_tag="CS526_29550"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(2236..2646)
                     /locus_tag="CS526_RS29735"
                     /old_locus_tag="CS526_29555"
     CDS             complement(2236..2646)
                     /locus_tag="CS526_RS29735"
                     /old_locus_tag="CS526_29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2646..2837)
                     /locus_tag="CS526_RS29740"
                     /old_locus_tag="CS526_29560"
     CDS             complement(2646..2837)
                     /locus_tag="CS526_RS29740"
                     /old_locus_tag="CS526_29560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3666..4022)
                     /locus_tag="CS526_RS29755"
                     /old_locus_tag="CS526_29575"
     CDS             complement(3666..4022)
                     /locus_tag="CS526_RS29755"
                     /old_locus_tag="CS526_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     Typing          2957..3366
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcggcaatca tcatcactat catgattttg ctgcattttt cttttacctc aaatgttgcc
       61 gcaacttggc aagttctaga tcacctttta ccaccaccat agtacaacaa acgccagaaa
      121 tgcacaatag cactgtgcac ttgtgcaaaa aaccataatg atataaaatg cacaaaagca
      181 cagtgctact gtgcaaaaaa agaatagttg gaggtcatat ggcgcttgtt cgctcaggaa
      241 ataaaaccaa gatttttgaa gcagatccga aatttaaccc agtttttgat acacaacaca
      301 agcctggcga tacgtgcacg tatgaaggaa tttacaaatg cacaggttgc ggtaaagaag
      361 taactatcgc aggagggaaa acacttccac cacaaaatca tcatcaacat agtcccggac
      421 agggggacat ccgctggcaa ctatttgttt atgcagagtg aggtatgaaa atggcagacc
      481 gtagaccact taacccgcat gtcgtcgtcc ctaaaccagg aggcggaact cagccacgag
      541 atcgtaataa taatggaaga gtccgagaga aacgttcaga tgcgggaaaa tcaagaaaat
      601 aagaaaagcc gccaaatagg ggtcagtttg gatatcggaa acttttgtac gttaagcgca
      661 ttatttaacc aatattgatg cgtaagggat tgattttacc tcagctacga tcgcttttaa
      721 gtcaccgctc tttctgtatc gcatttaggt tatccgatta aggaaaattc acgggaaacg
      781 gcgtatagcg cgggagtaca tcattgcgag aggtaagcct gttcaaaaca caggcttacc
      841 gtacaataat tctctatatc caaactgacc ccaagaagtc tgacactaac cccacacaaa
      901 tcgaatccgt tgcaagatga gacacacaag aaaagagcta gacgaaggtc gctccgctcc
      961 ccatggtttt tgtataccca ccatgggggc ataggtagtt tattaactaa ctccaagata
     1021 atgcgtgatc gcagccgtga gtacaccaca gcccacttgt ttagataaat ctaaaacccc
     1081 ttgtaggatt ctcttagtgt tccactcacc ttctgcatcc attggttgag atagtgcagc
     1141 actcaactgg ttcttaatct tttgcgaagt tatatcgtta tctattgagt tgatttcctc
     1201 cttggctttc tccaaggaag cctggagttc accgcgttct atttttaact tttcacgata
     1261 actgtctggt gtgtgtagtt cgttcccatc cgcatcgaga ttaataactt cgcctcgctc
     1321 accaacagta acaaagctct ctacgtgaga cagatcatca acaaccatca cattcctttt
     1381 cattcttaaa ccagtcactt ttgcacctgc accgatcttc ataaaactaa agcctgattt
     1441 atcgttcact cacatcacct cataagataa aaatgttaat aacgttttca cgagcaatta
     1501 tttaccataa cgcctcatta cgcgcaccgc tcaaacgctc tagaaacgat tctggggcgt
     1561 aatccattaa atcctacgtc ttaccccctg aaaacgcctc aaaatcgctc cagggcgttt
     1621 tacgggcatg aattgttatg caccagactg actggtggcg gatttcagcc tgaattcagg
     1681 ccagacaact caaaacccgc accaaaccag actgaaaacg gtgaacgggg acgcaattta
     1741 ttgcgtggcc gtgaagcgtt gccggcgcag ccggaaaaac ggttttcagc acgttcagga
     1801 atcgggttcg tcgacggccg gcaatcacgt tatgcaagtc gccggacgac tcaccacacg
     1861 attcaccggc gattcgactc gtgacttgac tctagagtcg ccaaggtcaa aaaaacacca
     1921 aaatgacgat tcgactctag agtcaagtcg tcataagtta ctgatagtct taagtgagtc
     1981 gtcgatgact ctatgacgag tcgtcggcaa gtcgtacacg aatcgccgac gacttggatt
     2041 caccttacag aaagtaatac tgcgtaatgt aataacccac aactaactga tattaatgaa
     2101 ttatcttcaa aattttaatt ttgcgtgggg tgtgggtatt tttagtggtg aggagcgttc
     2161 agagatctgg cgatttttgg gaaggttcag gaactccccg ggatggccgg agaggaaaac
     2221 ggaagcatga cggagtcagg attttttcca gaatttccac cacggggaat cgggtggtga
     2281 agaaggttcg tgcttgtgct ccagaagctg catagcctga cgaagggaat caatatgctc
     2341 atctcgagcc ttaacagccc ctttaagacc atcgttttca gcttctagta aagcaattct
     2401 atgttgtaaa tcatgtaaac caacgctgtt aacacctgta gatcctgcgt cactttttac
     2461 agtatcgatg acaccatcaa gagaaccaaa aactctaata agctctgaag tatcaataac
     2521 tttaatgatc ttcccgtgct ttacaacaga ggtgtaactt aacttcccac tgttaatcat
     2581 cttgtaaatt gtggggcgac taaccttaac aagccgtgcc gcttcactaa tagacactct
     2641 ggccatcagt taccatcctg taattcatcc agtctcgagg acagtgtacg gaaaagatgc
     2701 tccgcatcat cgtggagttt ctcacaaagg tcggcctcaa catcgagatc aagctcgtta
     2761 agcttctcca gcaggagcag agactgattc tggatgaatt tagccatgtt cagcgctgtt
     2821 ttctgttgtt tcgtcataag aagcctcaga gcgtcagtgc tcagtgtaaa accagtctct
     2881 tttccgcttc ctcgctcact cagtcgctac gctcctgccg ttcggctgcg gcgagcggta
     2941 ggactatgcg tataaatgct cgagaaaacc gagcggacgc gtgatgggca gaaggaaagg
     3001 ccgcttagcg gccgtttttc cataggctcc gcccccctga caagcatcat agaaattgac
     3061 gctcaaatca gtggtggcga aacccgacag gactataaag atatcaggcg tttccccctg
     3121 atggctccct cgtgcgctct cctgttcctg ccattcggtt taccggtgtc attccgctgt
     3181 tatggccgcg tttatctcat tccacgccag acactcagtt ccgggaaggc agttcgctcc
     3241 aagctggact gtatgcacga accccccgtt cagtccgact gctgcgtctt atccggtaac
     3301 tatcgtcttg agtccaaccc ggtaagacat gcaaaagcgc cactggcagc agccactggt
     3361 aactgattta gaggagttag tcttgaagtc atgcgcctgt taaggctaaa ctgaaagaac
     3421 aagttttggt gactgcgctc ctccaagcca gttaccttgg ttcaatcacc tgcaagtgtt
     3481 tgaaccttcg gaaaaaccgc cctgcaaggc ggtttttccg tttacagagc aagagattac
     3541 gcgtaccgaa accggatctc aagtgctcgt aaaagaaaat gatgatgacc tttagcggtt
     3601 acaaaaaaag aaggcacact attacagagg ccttcttcta gtttggttaa atttttttaa
     3661 aaaaatcatg cgaccgcatg ttttttttcc aaagattcag cattgtgctt cctttctata
     3721 atttcatgag cgggagtgac tctttctatt tcttcctgaa ggcgtgggct gctcgtaacc
     3781 tcccacaaat caacagactg ctgtatattt aaccagaact tagcggtagt atcgaatgct
     3841 ttagctaacc gaaacgccat atcggtagac agtttcctat tattgttaac caaagcactt
     3901 gcagaatttc tatgcacgtt taacatttca gccaagtcat tgatttttaa accacttggc
     3961 tcgagatatt cataaagaat aatatctcct acagtcgatg gttttctggt tgcttgggac
     4021 atgctttact cctgaattgg tcgcttctct ctgttagatg aggaagataa gtatttcttc
     4081 tgttaccagc tgtatcgatt agtaattatc ttaaatatag ttacgtcgcg ttactttatt
     4141 aatcattatt tgtgatgacg ttttaacgta ggtgaggaaa tgcctcacct acttgttttg
     4201 taagataaca gttccggtct gttacctatc ggtaatcatg tggatcgaga tacaaatcct
     4261 cgactccgtt ctcaccccac ttaaatatca agcggtactt atcattcacc cttatagatg
     4321 aataccctgt caatggaggg ttgagatttt caaatcggtt ccccggggga gaccgtaaat
     4381 ctccaatttt atcagcagca ttcagcatat ccagcttgcg acctaatgaa acactaatcg
     4441 tagctggaat ttttcgattg gctttaccgt atttataaaa ctggtatagc caatcgtctc
     4501 ggaactgaat
//
