LOCUS       NZ_CP024195             4660 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae isolate KSB1_5D plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP024195
VERSION     NZ_CP024195.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06112197
            Assembly: GCF_002741685.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlick,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Antimicrobial resistant Klebsiella pneumoniae carriage and infection
            in specialized geriatric care wards linked to acquisition in the
            referring hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Gorrie,C.L., Mirceta,M., Wick,R., Judd,L.M., Wyres,K.L.,
            Thomson,N.R., Strugnell,R.A., Pratt,N.F., Garlick,J.S., Watson,K.M.,
            Hunter,P.C., McGloughlin,S.A., Spelman,D.W., Jenney,A.W.J. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2017) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3052,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2017
            Assembly Method                   :: Unicycler v. 0.4.0
            Assembly Name                     :: KSB1_5D
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 83x
            Sequencing Technology             :: Illumina HiSeq and ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 03:14:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,735
            CDSs (total)                      :: 5,613
            Genes (coding)                    :: 5,446
            CDSs (with protein)               :: 5,446
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 167
            CDSs (without protein)            :: 167
            Pseudo Genes (ambiguous residues) :: 0 of 167
            Pseudo Genes (frameshifted)       :: 53 of 167
            Pseudo Genes (incomplete)         :: 113 of 167
            Pseudo Genes (internal stop)      :: 16 of 167
            Pseudo Genes (multiple problems)  :: 15 of 167
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024195.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="KSB1_5D"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="Australia: Melbourne"
                     /lat_lon="37.8457 S 144.9821 E"
                     /collection_date="2013"
                     /collected_by="University of Melbourne"
     gene            124..306
                     /locus_tag="CS526_RS29675"
                     /old_locus_tag="CS526_29500"
     CDS             124..306
                     /locus_tag="CS526_RS29675"
                     /old_locus_tag="CS526_29500"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080882243.1"
                     /translation="MGKELSLNKIYDKNSRYINDSRGHELGKEKKVFDFEVEMIKKRRE
                     DAIRKIIEDSKSLKW"
                     /name="hypothetical protein"
     gene            319..2019
                     /locus_tag="CS526_RS29680"
                     /old_locus_tag="CS526_29505"
     CDS             319..2019
                     /locus_tag="CS526_RS29680"
                     /old_locus_tag="CS526_29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697690.1"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage dCTP deaminase"
                     /protein_id="WP_225368539.1"
                     /translation="MSLARKLREKRSNPIHSANEDSSGGDSINQITSRQSMDLVIGFCG
                     AVGSGIKSIVDVTEEELDDLGYDVYRIRVSELMQKLFSEWRDDIVTPFDRYHKLQDFGD
                     ALRNKYYPHILSEAVIHEIKIEKGIDTSSKKNKKAFLVDQLKHQDEIELLRMVYQHNFY
                     LLGVIRSEVERKRNLRDEGLSKSEVDIVIHHDRKSESDYGQQTAKAILDSDYFVKNNQG
                     QRAHLKAKVKRFIGLIHGLNGLTPTTYEKGMYAAHSASLQSACLSRQVGAAILDVNGNL
                     ISVGRNDVPKFNGGLYSADDGVNDHRCVYKGGKCYNEVRKLKIKSKISQILMSESACEL
                     GVKLTEDQAEQLADVIYKKTPVSSIIEYSRSIHAEMDAITSLARLGNGGFSDKVLYTTT
                     FPCHNCARHIVAVGITKVVYIEPYEKSLALELHDDAITEVNEHGKVIFESFEGVSPRRY
                     QKFFFSTDERKDSFGNAEKYSTKYKNHIDIQFIDSYLDFENRVVDIFRTNTNIDSGNHV
                     GRTDSTINLSSQPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPDSK"
                     /name="anti-phage dCTP deaminase"
     gene            complement(2223..2336)
                     /locus_tag="CS526_RS31065"
                     /old_locus_tag="CS526_29515"
     CDS             complement(2223..2336)
                     /locus_tag="CS526_RS31065"
                     /old_locus_tag="CS526_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_080882241.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            2752..2943
                     /locus_tag="CS526_RS29700"
                     /old_locus_tag="CS526_29525"
     CDS             2752..2943
                     /locus_tag="CS526_RS29700"
                     /old_locus_tag="CS526_29525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2943..3344
                     /locus_tag="CS526_RS29705"
                     /old_locus_tag="CS526_29530"
     CDS             2943..3344
                     /locus_tag="CS526_RS29705"
                     /old_locus_tag="CS526_29530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4187..4396)
                     /locus_tag="CS526_RS29715"
                     /old_locus_tag="CS526_29535"
     CDS             complement(4187..4396)
                     /locus_tag="CS526_RS29715"
                     /old_locus_tag="CS526_29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     Typing          complement(2224..2631)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.355"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttttctcttg ccgaatttcc taatgtttag gataagaaaa aaaaatctct atcgattacg
       61 tttcttggct attgcctgtt ataaagataa cagtaaaatc catgcgtctt gcaggagggt
      121 gtgatgggta aagagctatc tctaaataaa atttatgata agaattctag atatattaat
      181 gattctagag ggcatgagct gggaaaggaa aagaaagttt tcgattttga agttgaaatg
      241 attaaaaaaa gaagagagga cgctataagg aagataattg aggattcaaa atcattaaaa
      301 tggtaagtga ggtcttttat gagcctggct agaaagttaa gagaaaaaag aagtaatccg
      361 attcatagtg ctaacgagga ctcctctgga ggggatagca taaaccaaat aacctccaga
      421 cagtctatgg atcttgttat aggattttgc ggtgctgttg gctcaggaat aaagtcaatt
      481 gttgatgtga ctgaagaaga actagatgat ctaggatatg atgtttatcg cataagagtt
      541 agcgagctta tgcaaaaact tttttcagaa tggcgtgatg atattgttac tccgtttgat
      601 agatatcata agctgcaaga ttttggtgat gcgttaagaa ataaatatta cccacatata
      661 ctatctgaag ctgtaatcca tgaaataaaa atagaaaaag gcattgacac ctcaagtaaa
      721 aaaaacaaaa aggctttttt ggtcgatcag ttgaagcatc aggatgaaat tgaactttta
      781 aggatggtct accaacataa tttttattta ctaggtgtaa ttagaagtga agtagaaaga
      841 aagcgtaatc ttagagatga agggctgagt aagtcagaag tcgatatagt tattcatcac
      901 gatagaaaat cagagagcga ttatggtcag cagactgcga aagctatact tgactctgat
      961 tattttgtta agaataatca aggacaaagg gcgcacctta aggctaaggt taagcgtttt
     1021 attggcttga tacatgggct aaatggttta actccaacca cgtatgaaaa aggtatgtat
     1081 gcagctcatt cagcatctct gcaatctgca tgcctttctc gtcaagttgg tgctgctata
     1141 ttggatgtta atgggaatct gatttccgtt gggcgtaatg atgtacctaa attcaatggt
     1201 gggctttata gtgcggatga tggtgtcaat gatcatcgtt gtgtatacaa aggtggtaaa
     1261 tgttataatg aagttagaaa actaaagatt aaaagcaaaa tttcacaaat acttatgtcg
     1321 gaaagcgctt gtgaacttgg tgttaaatta acagaagatc aagcagagca actggctgat
     1381 gtaatatata aaaaaacacc agtatcttct attatagagt actctcgctc aattcatgca
     1441 gagatggatg ctattacatc attagctaga ctaggtaatg gaggtttttc tgataaagtt
     1501 ttatatacaa ccacatttcc ttgtcataac tgcgcgagac atattgtcgc tgttggaata
     1561 acaaaagttg tttatataga gccatatgaa aaaagcctcg ctttagaatt gcatgatgat
     1621 gctataaccg aggtgaatga gcatgggaag gttatatttg aatcctttga aggtgtatca
     1681 cctcgtaggt atcaaaaatt cttcttttct acagatgaaa ggaaagatag tttcggtaac
     1741 gcagaaaagt actccactaa atataaaaat cacattgata tacagtttat tgatagctat
     1801 ttagatttcg aaaatcgagt cgttgatatt tttagaacta acacaaatat tgattctggc
     1861 aatcatgtcg ggagaactga ttcgactatt aatttatctt cccagcccgt accgcccgta
     1921 ccgcccgtac cgcccgtacc gcccgtaccg ccagtaccgc cagtaccgcc agtaccgcca
     1981 gtaccaccag taccaccagt accaccagac tcgaaatgaa gatctgttga ctctgatgtt
     2041 atacagagct taaccttgag acgagaaaga aaaaaaccgc ctttgcgggg cggttttttt
     2101 aaggttctag agtttgcggc tctatgaacc aaggtaactg gcttagagga gcgcagtcac
     2161 caaaacttgt tctttcagtt tagccttaac tggcgcataa cttcaagact aactcctcta
     2221 aatcagttac cggtggctgc tgccagtggt gcttttgcat gtcttaccgg gttggactca
     2281 agatgatagt taccggataa gacgcagcag tcggactgaa cggggggttc gtgcatacag
     2341 tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg
     2401 cggccataac agcggaatga caccggtaaa ccgaatggca ggaacaggag agcgcacgag
     2461 ggagccatca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccgccactg
     2521 atttgagcgt cagattccgt gatgctcgtc aggggggcgg agcctatgga aaaacggccg
     2581 ctgtgcggcc tttccttttc cccatcacga gaccgctcgg ttttctcgag cttttacacg
     2641 catgctcctg ccgctcgccg cagccgaacg gcaggagcgt agcgactgag tgagcgagga
     2701 agcggaaaag agactgttct tacactgagc actgacgctc tgaggcttct tatgacgaaa
     2761 caacagaaaa cagcgctgaa catggctaaa ttcatccaga atcagtctct gctcctgctg
     2821 gagaagctta acgagcttga tctcgatgtt gaggccgacc tttgtgagaa actccacgat
     2881 gatgcggagc atcttttccg tacactgtcc tcgagactgg atgaattaca ggatggtaac
     2941 tgatggccag agtgtctatt agtgaggctg cccgactgac gggtaagagt agaacaacac
     3001 tgcacaggtt gattaaagca ggtgacctgt ccacctgtag cggtgaacgc aatgcaaaaa
     3061 tggtagatac ttcagagctt ttacgtgtct ttggtccctt tgagcaaccg aagggtgaac
     3121 accattctgg acaggtctct gaacagcgtg atacagcctc tgctgaccag agtgaacagg
     3181 tgattcgtca tctgaaacag gagattgaac acctgaatac cttacttttg tccaaggatt
     3241 cgcacattga cagcctgaaa caggccatgc tccttctcga aaatcaccag gcaaaattaa
     3301 ctgaatcccc ggcgccatgg tggaaattct ggaaaaaatc ctgaccccgt catgcttccg
     3361 ttttcctctc cggccatccc ggggagttcc tgaaccttcc caaaaatcgt cagatctctg
     3421 aacgctcctc accactaaaa atacccacac cccacgcaaa attaatattt tgcagatttg
     3481 tatttaatat cagtgggtta ctttcctgaa atttcgtttt ttaaatttta tttatataga
     3541 tccgactcta tgacgactcg tcatagagtc cccggcgagt cgtcatagag tcggtaacga
     3601 ctcaggacta taaaaacaat aagttaataa cgacccgact ctagacacga ctccccgttt
     3661 ggttgttttt tagacgcggc gactctgggt acgactctag tgaagagtcg ccggtgaatc
     3721 gtgtggtgag tcgtccggcg acttgccata acgtgattgc cggccgtcga cgaacccgat
     3781 tcctgaacgt gctgaaaacc ggttttccgg ctgcgccggc aacgcttcac ggccacgcaa
     3841 taaattgcgt ccccgttcac cgttttcgga tggaaagaac gccggaaaca ggacttactc
     3901 tggctgaatg gcgtgaattt tcgcgctatg cacttgcgtg catactcatg catgccgtaa
     3961 aaacagagcc tgcgcgtttc tggcgggttt tcgggtggtt tgttgcctgt tttaccggtt
     4021 ttccgtcaga aacgccctga ggccgtttta gcggtgcgta caatcatgcc tttatgttaa
     4081 ataacagatg caaaaaagcc ccagaagggg cctgaaggtt ttcctgctaa gggacgaaac
     4141 gggccgcctc ccggcggcct gttcggcagc tccctttgcg accatcttat ttggtcagtt
     4201 cagccgtcat gtggacacgg ttattgaact gagcgctggt gattttgtat gacgcacctt
     4261 gttcagcggc ctgtgcggca atttttgctt cagcacgatc cagggtggag gcggtagcgc
     4321 tgacgctctg agcaaaagca ccgaaagaca tcatggatag agcggcaact gcaacgaaag
     4381 ttttgatgga tttcatggtc gtattcctta agtgattttg ttctgtatgg ggcgttgttg
     4441 ccctgatgtg ataaatgata ggacgaccat tagttgatta aaatcgaaac aatttgcgga
     4501 tatcattcaa aaaaaaagaa caagaattga gcgcatcaag accagaggtt tatctctgga
     4561 ttttaggtac caaatgaggt ggtggataga aatgttaaca ttgatgattt tattttaaca
     4621 aattgaaaaa taatggttag tttggcgtaa tctctcttgc
//
