LOCUS       NZ_CP024044             4660 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain QS17-0029 plasmid pMRSN479404_4.7,
            complete sequence.
ACCESSION   NZ_CP024044
VERSION     NZ_CP024044.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07792096
            Assembly: GCF_003073235.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Snesrud,E.
  TITLE     Sequencing of mcr-1 carrying Escherichia coli from Thailand
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Snesrud,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2017) Multidrug Resistant Organism Repository and
            Surveillance Network, Walter Reed Army Institute of Research, 503
            Robert Grant Ave., Silver Spring, MD 20910, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2017
            Assembly Method                   :: HGAP v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 01:13:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,590
            CDSs (total)                      :: 5,460
            Genes (coding)                    :: 5,305
            CDSs (with protein)               :: 5,305
            Genes (RNA)                       :: 130
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 76 of 155
            Pseudo Genes (incomplete)         :: 78 of 155
            Pseudo Genes (internal stop)      :: 17 of 155
            Pseudo Genes (multiple problems)  :: 16 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024044.1.
            Bacteria and source DNA available from The Multidrug Resistant
            Organism Repository and Surveillance Network (MRSN).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="QS17-0029"
                     /host="Homo sapiens"
                     /culture_collection="MRSN<USA>:479404"
                     /db_xref="taxon:573"
                     /plasmid="pMRSN479404_4.7"
                     /geo_loc_name="Thailand: Chonburi"
                     /lat_lon="12.6890 N 100.9820 E"
                     /collection_date="2017-04"
                     /collected_by="AFRIMS"
     gene            107..220
                     /locus_tag="CR230_RS30115"
                     /old_locus_tag="CR230_28715"
     CDS             107..220
                     /locus_tag="CR230_RS30115"
                     /old_locus_tag="CR230_28715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_080882241.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(424..2124)
                     /locus_tag="CR230_RS28855"
                     /old_locus_tag="CR230_28725"
     CDS             complement(424..2124)
                     /locus_tag="CR230_RS28855"
                     /old_locus_tag="CR230_28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697690.1"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage dCTP deaminase"
                     /protein_id="WP_225368539.1"
                     /translation="MSLARKLREKRSNPIHSANEDSSGGDSINQITSRQSMDLVIGFCG
                     AVGSGIKSIVDVTEEELDDLGYDVYRIRVSELMQKLFSEWRDDIVTPFDRYHKLQDFGD
                     ALRNKYYPHILSEAVIHEIKIEKGIDTSSKKNKKAFLVDQLKHQDEIELLRMVYQHNFY
                     LLGVIRSEVERKRNLRDEGLSKSEVDIVIHHDRKSESDYGQQTAKAILDSDYFVKNNQG
                     QRAHLKAKVKRFIGLIHGLNGLTPTTYEKGMYAAHSASLQSACLSRQVGAAILDVNGNL
                     ISVGRNDVPKFNGGLYSADDGVNDHRCVYKGGKCYNEVRKLKIKSKISQILMSESACEL
                     GVKLTEDQAEQLADVIYKKTPVSSIIEYSRSIHAEMDAITSLARLGNGGFSDKVLYTTT
                     FPCHNCARHIVAVGITKVVYIEPYEKSLALELHDDAITEVNEHGKVIFESFEGVSPRRY
                     QKFFFSTDERKDSFGNAEKYSTKYKNHIDIQFIDSYLDFENRVVDIFRTNTNIDSGNHV
                     GRTDSTINLSSQPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPDSK"
                     /name="anti-phage dCTP deaminase"
     gene            complement(2137..2319)
                     /locus_tag="CR230_RS28860"
                     /old_locus_tag="CR230_28730"
     CDS             complement(2137..2319)
                     /locus_tag="CR230_RS28860"
                     /old_locus_tag="CR230_28730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080882243.1"
                     /translation="MGKELSLNKIYDKNSRYINDSRGHELGKEKKVFDFEVEMIKKRRE
                     DAIRKIIEDSKSLKW"
                     /name="hypothetical protein"
     gene            2707..2916
                     /locus_tag="CR230_RS28865"
                     /old_locus_tag="CR230_28735"
     CDS             2707..2916
                     /locus_tag="CR230_RS28865"
                     /old_locus_tag="CR230_28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            complement(3759..4160)
                     /locus_tag="CR230_RS28875"
                     /old_locus_tag="CR230_28740"
     CDS             complement(3759..4160)
                     /locus_tag="CR230_RS28875"
                     /old_locus_tag="CR230_28740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4160..4351)
                     /locus_tag="CR230_RS28880"
                     /old_locus_tag="CR230_28745"
     CDS             complement(4160..4351)
                     /locus_tag="CR230_RS28880"
                     /old_locus_tag="CR230_28745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2..220
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.909"
                     /coverage="94.0"
                     /evalue="1.27e-95"
     Typing          4473..4660
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.122"
                     /coverage="99.0"
                     /evalue="5.27e-72"
ORIGIN
        1 cctgccattc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
       61 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
      121 cgttcagtcc gactgctgcg tcttatccgg taactatcat cttgagtcca acccggtaag
      181 acatgcaaaa gcaccactgg cagcagccac cggtaactga tttagaggag ttagtcttga
      241 agttatgcgc cagttaaggc taaactgaaa gaacaagttt tggtgactgc gctcctctaa
      301 gccagttacc ttggttcata gagccgcaaa ctctagaacc ttaaaaaaac cgccccgcaa
      361 aggcggtttt tttctttctc gtctcaaggt taagctctgt ataacatcag agtcaacaga
      421 tcttcatttc gagtctggtg gtactggtgg tactggtggt actggtggta ctggcggtac
      481 gggcggtacg ggcggtacgg gcggtacggg cggtacgggc ggtacgggcg gtacgggctg
      541 ggaagataaa ttaatagtcg aatcagttct cccgacatga ttgccagaat caatatttgt
      601 gttagttcta aaaatatcaa cgactcgatt ttcgaaatct aaatagctat caataaactg
      661 tatatcaatg tgatttttat atttagtgga gtacttttct gcgttaccga aactatcttt
      721 cctttcatct gtagaaaaga agaatttttg atacctacga ggtgatacac cttcaaagga
      781 ttcaaatata accttcccat gctcattcac ctcggttata gcatcatcat gcaattctaa
      841 agcgaggctt ttttcatatg gctctatata aacaactttt gttattccaa cagcgacaat
      901 atgtctcgcg cagttatgac aaggaaatgt ggttgtatat aaaactttat cagaaaaacc
      961 tccattacct agtctagcta atgatgtaat agcatccatc tctgcatgaa ttgagcgaga
     1021 gtactctata atagaagata ctggtgtttt tttatatatt acatcagcca gttgctctgc
     1081 ttgatcttct gttaatttaa caccaagttc acaagcgctt tccgacataa gtatttgtga
     1141 aattttgctt ttaatcttta gttttctaac ttcattataa catttaccac ctttgtatac
     1201 acaacgatga tcattgacac catcatccgc actataaagc ccaccattga atttaggtac
     1261 atcattacgc ccaacggaaa tcagattccc attaacatcc aatatagcag caccaacttg
     1321 acgagaaagg catgcagatt gcagagatgc tgaatgagct gcatacatac ctttttcata
     1381 cgtggttgga gttaaaccat ttagcccatg tatcaagcca ataaaacgct taaccttagc
     1441 cttaaggtgc gccctttgtc cttgattatt cttaacaaaa taatcagagt caagtatagc
     1501 tttcgcagtc tgctgaccat aatcgctctc tgattttcta tcgtgatgaa taactatatc
     1561 gacttctgac ttactcagcc cttcatctct aagattacgc tttctttcta cttcacttct
     1621 aattacacct agtaaataaa aattatgttg gtagaccatc cttaaaagtt caatttcatc
     1681 ctgatgcttc aactgatcga ccaaaaaagc ctttttgttt tttttacttg aggtgtcaat
     1741 gcctttttct atttttattt catggattac agcttcagat agtatatgtg ggtaatattt
     1801 atttcttaac gcatcaccaa aatcttgcag cttatgatat ctatcaaacg gagtaacaat
     1861 atcatcacgc cattctgaaa aaagtttttg cataagctcg ctaactctta tgcgataaac
     1921 atcatatcct agatcatcta gttcttcttc agtcacatca acaattgact ttattcctga
     1981 gccaacagca ccgcaaaatc ctataacaag atccatagac tgtctggagg ttatttggtt
     2041 tatgctatcc cctccagagg agtcctcgtt agcactatga atcggattac ttcttttttc
     2101 tcttaacttt ctagccaggc tcataaaaga cctcacttac cattttaatg attttgaatc
     2161 ctcaattatc ttccttatag cgtcctctct tcttttttta atcatttcaa cttcaaaatc
     2221 gaaaactttc ttttcctttc ccagctcatg ccctctagaa tcattaatat atctagaatt
     2281 cttatcataa attttattta gagatagctc tttacccatc acaccctcct gcaagacgca
     2341 tggattttac tgttatcttt ataacaggca atagccaaga aacgtaatcg atagagattt
     2401 ttttttctta tcctaaacat taggaaattc ggcaagagaa aagcaagaga gattacgcca
     2461 aactaaccat tatttttcaa tttgttaaaa taaaatcatc aatgttaaca tttctatcca
     2521 ccacctcatt tggtacctaa aatccagaga taaacctctg gtcttgatgc gctcaattct
     2581 tgttcttttt ttttgaatga tatccgcaaa ttgtttcgat tttaatcaac taatggtcgt
     2641 cctatcattt atcacatcag ggcaacaacg ccccatacag aacaaaatca cttaaggaat
     2701 acgaccatga aatccatcaa aactttcgtt gcagttgccg ctctatccat gatgtctttc
     2761 ggtgcttttg ctcagagcgt cagcgctacc gcctccaccc tggatcgtgc tgaagcaaaa
     2821 attgccgcac aggccgctga acaaggtgcg tcatacaaaa tcaccagcgc tcagttcaat
     2881 aaccgtgtcc acatgacggc tgaactgacc aaataagatg gtcgcaaagg gagctgccga
     2941 acaggccgcc gggaggcggc ccgtttcgtc ccttagcagg aaaaccttca ggccccttct
     3001 ggggcttttt tgcatctgtt atttaacata aaggcatgat tgtacgcacc gctaaaacgg
     3061 cctcagggcg tttctgacgg aaaaccggta aaacaggcaa caaaccaccc gaaaacccgc
     3121 cagaaacgcg caggctctgt ttttacggca tgcatgagta tgcacgcaag tgcatagcgc
     3181 gaaaattcac gccattcagc cagagtaagt cctgtttccg gcgttctttc catccgaaaa
     3241 cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa
     3301 ccggttttca gcacgttcag gaatcgggtt cgtcgacggc cggcaatcac gttatggcaa
     3361 gtcgccggac gactcaccac acgattcacc ggcgactctt cactagagtc gtacccagag
     3421 tcgccgcgtc taaaaaacaa ccaaacgggg agtcgtgtct agagtcgggt cgttattaac
     3481 ttattgtttt tatagtcctg agtcgttacc gactctatga cgactcgccg gggactctat
     3541 gacgagtcgt catagagtcg gatctatata aataaaattt aaaaaacgaa atttcaggaa
     3601 agtaacccac tgatattaaa tacaaatctg caaaatatta attttgcgtg gggtgtgggt
     3661 atttttagtg gtgaggagcg ttcagagatc tgacgatttt tgggaaggtt caggaactcc
     3721 ccgggatggc cggagaggaa aactgaagca tgacggggtc aggatttttt ccagaatttc
     3781 caccatggcg ccggggattc agttaatttt gcctggtgat tttcgagaag gagcatggcc
     3841 tgtttcaggc tgtcaatgtg cgaatccttg gacaaaagta aggtattcag gtgttcaatc
     3901 tcctgtttca gatgacgaat cacctgttca ctctggtcag cagaggctgt atcacgctgt
     3961 tcagagacct gtccagaatg gtgttcaccc ttcggttgct caaagggacc aaagacacgt
     4021 aaaagctctg aagtatctac catttttgca ttgcgttcac cgctacaggt ggacaggtca
     4081 cctgctttaa tcaacctgtg cagtgttgtt ctactcttac ccgtcagtcg ggcagcctca
     4141 ctaatagaca ctctggccat cagttaccat cctgtaattc atccagtctc gaggacagtg
     4201 tacggaaaag atgctccgca tcatcgtgga gtttctcaca aaggtcggcc tcaacatcga
     4261 gatcaagctc gttaagcttc tccagcagga gcagagactg attctggatg aatttagcca
     4321 tgttcagcgc tgttttctgt tgtttcgtca taagaagcct cagagcgtca gtgctcagtg
     4381 taagaacagt ctcttttccg cttcctcgct cactcagtcg ctacgctcct gccgttcggc
     4441 tgcggcgagc ggcaggagca tgcgtgtaaa agctcgagaa aaccgagcgg tctcgtgatg
     4501 gggaaaagga aaggccgcac agcggccgtt tttccatagg ctccgccccc ctgacgagca
     4561 tcacggaatc tgacgctcaa atcagtggcg gcgaaacccg acaggactat aaagatacca
     4621 ggcgtttccc cctgatggct ccctcgtgcg ctctcctgtt
//
