LOCUS       NZ_CP024043             5251 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain QS17-0029 plasmid pMRSN479404_5.3,
            complete sequence.
ACCESSION   NZ_CP024043
VERSION     NZ_CP024043.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07792096
            Assembly: GCF_003073235.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Snesrud,E.
  TITLE     Sequencing of mcr-1 carrying Escherichia coli from Thailand
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Snesrud,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2017) Multidrug Resistant Organism Repository and
            Surveillance Network, Walter Reed Army Institute of Research, 503
            Robert Grant Ave., Silver Spring, MD 20910, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2017
            Assembly Method                   :: HGAP v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 01:13:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,590
            CDSs (total)                      :: 5,460
            Genes (coding)                    :: 5,305
            CDSs (with protein)               :: 5,305
            Genes (RNA)                       :: 130
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 76 of 155
            Pseudo Genes (incomplete)         :: 78 of 155
            Pseudo Genes (internal stop)      :: 17 of 155
            Pseudo Genes (multiple problems)  :: 16 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024043.1.
            Bacteria and source DNA available from The Multidrug Resistant
            Organism Repository and Surveillance Network (MRSN).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="QS17-0029"
                     /host="Homo sapiens"
                     /culture_collection="MRSN<USA>:479404"
                     /db_xref="taxon:573"
                     /plasmid="pMRSN479404_5.3"
                     /geo_loc_name="Thailand: Chonburi"
                     /lat_lon="12.6890 N 100.9820 E"
                     /collection_date="2017-04"
                     /collected_by="AFRIMS"
     gene            464..625
                     /locus_tag="CR230_RS30105"
                     /old_locus_tag="CR230_28690"
     CDS             464..625
                     /locus_tag="CR230_RS30105"
                     /old_locus_tag="CR230_28690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            complement(778..1398)
                     /locus_tag="CR230_RS28820"
                     /old_locus_tag="CR230_28695"
     CDS             complement(778..1398)
                     /locus_tag="CR230_RS28820"
                     /old_locus_tag="CR230_28695"
                     /inference="COORDINATES: protein motif:HMM:NF025084.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(1391..2746)
                     /locus_tag="CR230_RS28825"
                     /old_locus_tag="CR230_28700"
     CDS             complement(1391..2746)
                     /locus_tag="CR230_RS28825"
                     /old_locus_tag="CR230_28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     gene            complement(3379..3591)
                     /locus_tag="CR230_RS29125"
     CDS             complement(3379..3591)
                     /locus_tag="CR230_RS29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3595..4080)
                     /locus_tag="CR230_RS29130"
     CDS             complement(3595..4080)
                     /locus_tag="CR230_RS29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<4118..4795)
                     /locus_tag="CR230_RS30110"
                     /pseudo=""
     CDS             complement(<4118..4795)
                     /locus_tag="CR230_RS30110"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4758..5078)
                     /locus_tag="CR230_RS28835"
                     /old_locus_tag="CR230_28710"
     CDS             complement(4758..5078)
                     /locus_tag="CR230_RS28835"
                     /old_locus_tag="CR230_28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          61..574
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3264..4768)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.215"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tatgttggca tgagtgcggt aatcaggaaa gtgcttcatg tgacagagga aaatgcagct
       61 gcgggcgtaa ggcagaaaat ggaagttcag aggatatgac gcctcctcgc tcactcagtc
      121 gctgccgctc ctgccgttcg gctgcggcgg gcgttacctg cacacggaga agagcgaagg
      181 catatggcat gacaaacggg gcagaaaaag gatgcgccgc tgccgttttt ccataggctc
      241 cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca
      301 ggactataaa gataccaggc gtttccccct ggtggctccc tcgtgcgctc tcctgttcct
      361 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct
      421 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
      481 tcagtccgac cgctgcgcct tctccggtaa ctatcatctt gagtccaacc cggaaagaca
      541 cggttcatcg ccactggcag cagccattgg taacggatta gaggaagtga gtcttgaagt
      601 tctgcgccag ttaaggctaa actgaaagga caatttttgg tgactgcgct ccctctaagc
      661 cagttacccg gtacataagt tggtagctca tgtaccttcc aaaaaccacc gttggtagcg
      721 gtggttttta tttttcatag attacagcct gtaataagcc atggtcaatg gattacttca
      781 aaagtcgtca atcgccttaa gtagctcatg gaatgatgta taacatggaa atagataagg
      841 ttcgtgcgtg tttttataat tagttttagc gtaaatatct agctttcttg cgttatcttt
      901 cgcagtacca atcctctctc ttatagcaag gtaagttagt ttactacctt tatcatagtt
      961 agttattaat ttttttagcg gggagctaga catcaaatta tcatacgacg aaaaatcttt
     1021 ggttgtgaag ccgaaatgca acaataacca taattcaaaa caaacacagg atattgctat
     1081 atttatccca tgcctattag ctttattcca tgccttttga tgacatgaaa tagacgaagg
     1141 cgtcagatgc tcttgatcat aaacaaccca aaagacgtca ccttctgcgg aagatgatga
     1201 attctttttc tttatcgcct catccaccaa ctgctcaggt gtatttttcc ttgttttagg
     1261 tattttaaat acacttaaag ttcttttaga tctgtctgaa atataagcct caaggtataa
     1321 tggttcggtt ttctcaccct cacagtatat atacattact tgtgggactt tcttattatt
     1381 tccatgtttt ttactcatca gaaagatccc cgtcaaactc ttcaaaaaaa ccatcagaca
     1441 attctttgac gtcaatatcc ggcattacca aggatatatc accagatata aatgaggcta
     1501 cctttaagta gtttatgtcg ggaaccccac caaacctacc ctcatcatac caagctgcgt
     1561 aaggtgttgt tgtttttatc tttttcttat caaaatcatc taagctataa agagacgacg
     1621 cacctttatt tttttcacaa aaccatatct gatctcttct cattttctct ggagccatta
     1681 actgcatatt atgggttgag aatacaagtt gtgagccttt tttattgaca tcaggatcat
     1741 taaataactt aacaatcaag tcagcaatat gcgggtgcaa gctattatct aactcatcaa
     1801 caattacaac catactatca ttaaacgcag atctaattag aggtatgatt tcaaataatt
     1861 tttgggtgcc ttcagactct ctccccatag ggaaagaaac actcccccct tcatcagtag
     1921 gatgagagaa catataacga tatttataat cttcttcaat tatctccttt atttcttgag
     1981 ggatttcctc atccattttt attggtattt ccgcctcaac ctcccgtaca gatataccgg
     2041 aaacaccagt gtcaaccatg cataaaacct tggccgtttc atttacgacg tcctccctct
     2101 ccccaaaaga agatattctt atcttttcat ttaaacctat atgtctaatt ccctttctaa
     2161 aaaagttata agcttcttta attatgtctg gtgatgcggc attattacct gctttcgcta
     2221 agtatgaatt attagggaag aatggtattt ttttaatacc tcctttataa tgcccaccaa
     2281 acttgattgt ttcccaggtg tcgctctcat ctctagtaaa taaatttgct ttaaccctag
     2341 atggataata gtctaatgat tcaaataaaa tccgattttt aataaaagca acttcataac
     2401 tgaaccgatt cccctcaaca gaataaaatt cagcctcaaa ctttacagga gcctcattcg
     2461 tagccgaaga aagagcatat ggctcatagc agggaatccg tttaccttct ttcaaatcac
     2521 cgctttctgt tgcgataaat tttatagctt caaaagccat gagaacgtta gatttaccag
     2581 acgcattagc accataaatc ccagcactca tgagcgttcc aacattcatt ccatcatttt
     2641 tatagatatg agttgttaaa tggttactag aaccggatgc gctcaagttt aatgtcgatt
     2701 tttccttaac cgacctaaaa ttttcaatag tcaaataaac aaacatgcat acctcctaac
     2761 ggggattttt cgccaaaaaa gcgaaaaatc aatcatagga aagatatcct atatgtttta
     2821 attttggaac aacatcttcc ttaaaaaacg ttgtgctatt tataattaca cgctatttac
     2881 cataacatct gttgcccgca ccgctccgga caggttcgcc acaaacgggg cggcagtcag
     2941 gtaaaaccgg caacaaacca cccgatacac cgccccgttc gtgacagata aattttaccc
     3001 ggctgcatgg tcatgcagcc agatgcatac ggtctgattt caggccagaa tccggatgaa
     3061 cgccttaaaa acaggaaaat gcttctccag ccagtcccag atctccagcg ccatgtacag
     3121 gcaattcagc accgtctcaa tcacgttaaa cagtctcata atccctccgt tgtatacgct
     3181 gtgatggaaa catcacgacc gtatttagcg ggaaatttat gagcccttct tcttggggtt
     3241 tgtcactgga tgcgcgcagt cagcccagcg tcggaccgtc atagctccgc tgccgggaac
     3301 gggtttccgc cagctctttt tcatgctgtt tctgctcgta tgcccgttcg gcggccaccg
     3361 ccatttcatg ccgctgcact actggctcaa acgctgtaac tgcctgctca agttcgccac
     3421 ctgctgactc aagcgcgtga cgcgctcgtt cagcgccgcg ttctcccgtt gcataagacc
     3481 agacatcttc tgccattccg cgaaggcgct ctcccactcg tccagccttt gcgagtagtc
     3541 ctgatgtagc tgctctaatg cggtcagcaa ctgtttttcc agttccgtca tgggctattt
     3601 cctctccagt accatccagt tcccttcctc cccgtaagct cccgctttcg ggttcacttt
     3661 gacgcaggac agtttcttct ccccgcagag cgacagctgc acgccgtggt tcttctccga
     3721 cagcatggcc tgcgtccgct cctgcgcccg gatagtctgg taattcccgg tgatctgctg
     3781 cccctgccac cacagaatgc tcccgctcgt ggcgatcagc agtaccgaga ccagggtgat
     3841 ggtcagccac gtccggccga ccatacgcag cacgctcagc cggttcgact gcatcgccgc
     3901 ggtcataccc tgctcgtggg cgctgatggc gtcgctgatt ctcttcgcgc ttgcgctcag
     3961 ttccgctttt acagactgct cgtgctcgct gaatgcggct ttcagcatct cgccggtact
     4021 ctgctgctgc gctttcgatt gctgctccaa gtccttcgcc agcgctaaaa gactgctcat
     4081 aaatggctcc cttcagtcgg atgtttcgcc ccccgtccgg gtcggcaatg ctgatactgt
     4141 tttttgtggt gcggaccacc tcaaaacctg ccgctgtgag cgcccctgtg acgtcctcgc
     4201 gcgttttgag ctccccggat gaggctaagg ccagcaaccc acgtgtaatc gcctgagcgg
     4261 cttccagctt cgtttccggc agtgcggacg gcgtgaccag taaccgtcgg ttctccgggg
     4321 cgtttggatc atgcagcccc agcctgccgt tcaccacggt ctgccaggca tcgatgcgcg
     4381 gccggtcggc gcgatcgtag tacggctgca ggcgtttccc ggtcagcagt tcagtgttgg
     4441 ggatcaggaa attcagctcc agccgcccct tgtcggtgtg ctccacccac agaatgctgt
     4501 actggtcttt atcgagcccg ggcatcagaa cccgctcaaa gctggccatg atctgctccc
     4561 gctgcccggg cggcagttca gcctccgcga acgacagcac gccggaggtg tactttttcg
     4621 catacggcga ggcatcgata agctcccgga cttcctccgg ttttccctgc agcacggtcg
     4681 ccccctcgcg ctgccggtct tttcccagca ggtaatccac cggaccggca ccaccaccgc
     4741 gcccccgggg gtgaaattta acgatcatca tccccccctt tttccagcac cgcatgccgc
     4801 agccgctcca gtcccgcgtc gattgccatc agtgcggcca caatctgcac ccggtcatga
     4861 ccggcaccac cggcattcac tttccgggcg atctggttca ggttgttccc cataccggca
     4921 agctggcgca gcagcgccgg cgatatcgac ggcagtctgc cggcacgcgc cggcttctca
     4981 tcgaggcagg tctgacgcat ccacgccgcc agctgcttgc cgtcgcaccg ctcgagcagc
     5041 cgccggtgtt cgtcttccgt cacccacatc gtgagcatct tgttgcgttt gtctgccaga
     5101 gtagcctcct gataacaccg ggacgggcgg gaaatgtttc ccggtcggca cggtgttgga
     5161 caagccgcag gcgcgtcagt cgcttttagc gggtttcggg gcgcagccct gaaccagtca
     5221 cgtagcgcta gcggagtgta tactggctta t
//
