LOCUS       NZ_CP024042             6141 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain QS17-0029 plasmid pMR0617oxa, complete
            sequence.
ACCESSION   NZ_CP024042
VERSION     NZ_CP024042.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07792096
            Assembly: GCF_003073235.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Snesrud,E.
  TITLE     Sequencing of mcr-1 carrying Escherichia coli from Thailand
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Snesrud,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2017) Multidrug Resistant Organism Repository and
            Surveillance Network, Walter Reed Army Institute of Research, 503
            Robert Grant Ave., Silver Spring, MD 20910, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2017
            Assembly Method                   :: HGAP v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 01:13:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,590
            CDSs (total)                      :: 5,460
            Genes (coding)                    :: 5,305
            CDSs (with protein)               :: 5,305
            Genes (RNA)                       :: 130
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 76 of 155
            Pseudo Genes (incomplete)         :: 78 of 155
            Pseudo Genes (internal stop)      :: 17 of 155
            Pseudo Genes (multiple problems)  :: 16 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP024042.1.
            Bacteria and source DNA available from The Multidrug Resistant
            Organism Repository and Surveillance Network (MRSN).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="QS17-0029"
                     /host="Homo sapiens"
                     /culture_collection="MRSN<USA>:479404"
                     /db_xref="taxon:573"
                     /plasmid="pMR0617oxa"
                     /geo_loc_name="Thailand: Chonburi"
                     /lat_lon="12.6890 N 100.9820 E"
                     /collection_date="2017-04"
                     /collected_by="AFRIMS"
     gene            join(5031..6141,1..344)
                     /locus_tag="CR230_RS30010"
     CDS             join(5031..6141,1..344)
                     /locus_tag="CR230_RS30010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(626..907)
                     /locus_tag="CR230_RS28775"
                     /old_locus_tag="CR230_28650"
     CDS             complement(626..907)
                     /locus_tag="CR230_RS28775"
                     /old_locus_tag="CR230_28650"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            1251..2048
                     /locus_tag="CR230_RS28780"
                     /old_locus_tag="CR230_28655"
     CDS             1251..2048
                     /locus_tag="CR230_RS28780"
                     /old_locus_tag="CR230_28655"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            complement(2455..>2607)
                     /locus_tag="CR230_RS28785"
                     /old_locus_tag="CR230_28660"
                     /pseudo=""
     CDS             complement(2455..>2607)
                     /locus_tag="CR230_RS28785"
                     /old_locus_tag="CR230_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            2983..3930
                     /locus_tag="CR230_RS28790"
                     /old_locus_tag="CR230_28665"
     CDS             2983..3930
                     /locus_tag="CR230_RS28790"
                     /old_locus_tag="CR230_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            4338..4730
                     /locus_tag="CR230_RS28795"
                     /old_locus_tag="CR230_28670"
     CDS             4338..4730
                     /locus_tag="CR230_RS28795"
                     /old_locus_tag="CR230_28670"
                     /inference="COORDINATES: protein motif:HMM:NF037437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            4712..5050
                     /locus_tag="CR230_RS28800"
                     /old_locus_tag="CR230_28675"
     CDS             4712..5050
                     /locus_tag="CR230_RS28800"
                     /old_locus_tag="CR230_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             1252..2045
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2996..3917
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          5032..6140
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.108"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccgaaggggt actaaatgac gggattggag cgggaattgc tctgcgcgtt cgaggctttg
       61 cagagcgcat acgagcagca gcacaaggca tggcaggaaa gctacgcgaa cttggcgagc
      121 atgtacgaga gcaccaaaca agaggccgag atcagcaggg cagcagcaga gcgattgacg
      181 aggcaggtga acggcttaac gcagcaagtg gacggcttga gcaagcagct ggagggcttg
      241 accgggctag tcagcaagtt gcaaaagaaa gggaacacag gctcctgaag gcactggatc
      301 gaggctggga gcgtggagac agatcgcgag gctttgagcg ttgacggaac aaaacggccg
      361 tcagttataa aaaaaggaaa gtgctatcac tttcctttta tcggcgggaa ttatcgttga
      421 gttcttctat gccatttttc atagtagtaa ctgtaatctt catctctacc accagattca
      481 atccagtcat catactcagg gctatttttg aaatctgaat cagacatgct aactccttgt
      541 tattagttgg cttctatgga agctagcgca ccgtgaagtt atgatcaacg atgtagtcca
      601 catcattgtc gaaatgtgcg ttcagctagt cttcggtgtt ttctgcttta tttctagcgg
      661 cgattctcgc gtctgcccat gcggcagact ttttagcctg ttctcgttgt tcttcggttg
      721 aagactcgct gttgagaact ttgttgctcg tttgtttgac caccgaatcc aaacctgttg
      781 ttgctgcaaa atgagctagg ttaacaactc ccttacctaa caacttggca ctagcgattg
      841 cggtttcttt agccccaaaa gcatcagaca cctcctcaag cgctttagat aggttaaaaa
      901 atcccatact ccccctaaga atatcatcaa taaaattgag tgttgctctg tggataactt
      961 gcagagttta ttaagtatca ttgcagcaaa gatgaaatca atgatttatc aaaaatgatt
     1021 gaaaggtggt tgtaaataat gttacaatgt gtgaggagca gtctaaattc ttcgtgaaat
     1081 agtgattttt gaagctaata aaaaacacac gtggaattta ggtaatagtt aaagcttgtg
     1141 cctgattgtt gccacaattg gtcagcagtt tgctagggaa tgagagggca gagaatgata
     1201 agcttagcac ttaatttgta tggatatatt gcattaagca aggggacgtt atgcgtgtat
     1261 tagccttatc ggctgtgttt ttggtggcat cgattatcgg aatgccagcg gtagcaaagg
     1321 aatggcaaga aaacaaaagt tggaatgctc actttactga acataaatca cagggcgtag
     1381 ttgtgctctg gaatgagaat aagcagcaag gatttaccaa taatcttaaa cgggcgaacc
     1441 aagcattttt acccgcatct acctttaaaa ttcccaatag cttgatcgcc ctcgatttgg
     1501 gcgtggttaa ggatgaacac caagtcttta agtgggatgg acagacgcgt gatatcgccg
     1561 cttggaatcg tgaccatgac ttaattaccg cgatgaagta ctcagttgtg cctgtttatc
     1621 aagaatttgc ccgccaaatt ggtgaggcac gtatgagtaa aatgctgcac gccttcgatt
     1681 atggcaatga ggatatctcg ggcaatgtag acagtttttg gctcgatggt ggtattcgca
     1741 tttcggctac ccagcaaatc gcttttttac gcaagctgta tcacaacaag ctgcacgttt
     1801 ctgagcgtag tcagcgcatc gtgaaacaag ccatgctgac cgaagccaat ggcgactata
     1861 ttattcgggc taaaacggga tactcgacta gtatcgaacc taagattggc tggtgggttg
     1921 gttgggttga acttgatgat aatgtgtggt tttttgcgat gaatatggat atgcccacat
     1981 cggatggttt agggctgcgc caagccatca caaaagaagt gctcaaacag gagaaaatta
     2041 ttccctagaa gtggttagcg cgtatttgtg tgaaatagcc gtcatataag ctgtaaagat
     2101 atatggacaa aatgctgatg ctcgatgcgc ttgtagttaa tggctcatac ttaatcggtc
     2161 aggcctaatc gacttaagct ccgcgcgtta actttcagcc tgagcattag catcacattc
     2221 ctaaacgtaa aaagcctagc caatttgcta ggcttttttg ttatctcaat ggccatggtc
     2281 actcaacatg ggggtgtgaa taaaggagtg gcgatgtcag taaaggtgaa tgccgctgcg
     2341 ataaagtgcc tagcccatca aggattgtag gctttcttgg ctgctaaagc gtttgacgaa
     2401 aaagtccaag aaggcgctga tattggttct ccatcatttt catctaccgg tttactatag
     2461 gtcaaccagg ccgtccttgc cggcgcttgg gacgcagacg atggcatcaa atgcctcgct
     2521 caaattcgtt tctacagagg cgctttggga ccgcattcgc tttgctttca tgggggcatc
     2581 tgtcaatgtc agacatgaat cctccgatat aggttgattt ttgatctctt caacacttgg
     2641 tgttgtccga ctagagagac ttatatggat cagtattctt gcactgacaa tggtgccact
     2701 caatcccaag gatcgaccta gttagaggcg tagatcaagc aattcaaaac agcgcagaag
     2761 cagaaggagg tggaaatatg ttccagggtg aaaggttaca tgcaactaac gttgctctct
     2821 aggatgtgcc tgctgtaacg tattgtccag aaaagacaac cccccgttat cgcttgcatc
     2881 ttggcggagc tgcgaaaaac gggggccgtg acgaatcaca agagaagtct atcacatgga
     2941 tcatgccaaa caaccccacg ccgccaataa gcgcggtggt gcttgagaaa gtgcgcacag
     3001 cttgagctgt ttcgggagcg cctgccccgg aaaccctact attccgacga gcttacaacg
     3061 ggcctacgga ttgcggatgt tgcccgcgcc cttggtgcgc gttacatcca gccgaacggg
     3121 ccaacgcacc gccactggat cgtttttgac gtagaccatg cagccgctac gttatcttgg
     3181 gatgacgtag gcgctccagc ccctaacatt accgtcacga acaaggccaa cggccacgcg
     3241 catctgatct acggactgga tacgccgatc cgcactgccc cggatggaaa tgcagcccct
     3301 ctgcggtatg ccgccgccat cgaggccgcg ctccgggaga aattaggggc ggatatgggc
     3361 tactccgggc tgatctgcaa gaaccccctg catgagcact ggctggtgca ggtatgggaa
     3421 ccccggctct acgatttagc ctggctatcc gattacctcg atctgagctc ctacaatggc
     3481 cgcaaatcgc ttcctgagta cgggcttggg cgaaactgta ccctgttcga gaaaacgcgc
     3541 ctgtgggcct ataaagcgat ccggcagggc tggccggatt accccgaatg gttggccgct
     3601 tgtgttgacc gggcatcagg atataacgcc cagttcgagc agccgctccc cgcaaacgaa
     3661 gtccggcaca cggccaagtc cattgccaaa tggacgcacc agcacctaag ccccgcaggg
     3721 ttcagagagg agcaggcccg gagaggtgcg aagggcggca aggtatccaa gggaggggga
     3781 aggccgagca atagcggtaa ggacaagtcc gatctattgc cggaggttct gcggcttaag
     3841 gctcaggggt acaccaaccg cgatatagcg gacgatcttc aaatctcccc cagtacggtg
     3901 agcgtttacc taaagcgtga tcgtccgtga tcgaaacaag ccttatcaga taatagcccc
     3961 ctttaggggg gttcctgcgc tcttgtcttt tagccttaaa aaatcctttt agcgcggatc
     4021 tttggcgagg tggacaactg cgcctgtccg tccatcgccc ccctgttttt tcggggggtg
     4081 tgggcggcag cagttatcca cggagccgct ctttcccttg atcttgctct agggcttgca
     4141 gtatcccgcc catggttgaa taatgtacta agcaaggcta cacagaggcc cctctacctg
     4201 cttcgcggtc tcgggcctct gtggccttgt tcaggggtgc tacgcccccc cgaaaccccc
     4261 tgtggccccc gctcgatccg ccccttcggg acggtctgcg cggtggcttc ttggctctgg
     4321 agaatggacg aggccctatg tcaacaacac agatagagaa agattcgaga aggaaggcag
     4381 tcaatggcgt tatttctagt ttccaactag agggggaggc cataaccgat gaattcaaaa
     4441 ttctacttga agattatatc aatggaaaaa tttccaccga tgaggtgtta gctatggtaa
     4501 gtgacgaacc cacaacaacg gaatacgact acgaaatcac cccccgcact ctcggtcttg
     4561 gcgggggctg gaatctgcgt ttgctggaga acggcgaaga ggtcggcggg ggtgttttcc
     4621 ccttgccgga gcactgcgat tttcgggatg aaaaggcgct ccagacgcta ctcgatagcc
     4681 tctatgagga cgccttagcg gaggcctctg catggctggc cagccgttaa agcgcctccg
     4741 ctctatcaag atccgggtct cagatgcgga gcttgagcgc ctgcgggagc tctgccccaa
     4801 ggcccaactg gcggagtgga tgcgggagca atgtctcggt gttgcgcaac cccagcgccg
     4861 aacgccggcg cctaccgtag atccggcgct cttgcggcag ttggccggaa tgggcaacaa
     4921 cctgaaccag atcgcccggc gcgttaattc cggggagtgg gggccgttgg atcggctgcg
     4981 catcatagcg gagctgtccg ctataggccg cgagctggag gaactgcacc atgatcatca
     5041 aattccataa ccgtggggta ggccggggct ccgggcctgt tggctacttg ttgggccgtg
     5101 acgggaaccg ggaaggcgca acgctcgacc ggggcaaccc tgcggtgatt gaagccctga
     5161 ttgattccag tccctacgcc aaaaaataca cctctggcgt tctgtcgttt gccgaggctg
     5221 atttacccag ggcgaccaag gacaagctga tgagcgactt tgaaaaggcg ctcctgcccg
     5281 gactggaggg caaccagtac gctgttttgt gggtagagca ccgcgacaag gggcggctgg
     5341 agctgaattt tgtagtcccg aacatcgaat tgcagagcgg gaagcggctg caaccctact
     5401 tccacaaggc cgacaatccg cgcctcgatg cgtggcgaac cgtggtaaac ggggagctga
     5461 aattgcacga cccggacgat ccgttaaacc gtcaggccgt tatcacgcca aaggatttgc
     5521 cgagggggca caaggaggcc gctcaggcca ttacagacgg gcttttacgc atggccggag
     5581 caggggagat ccagagccgc gatgatgtgc gcaaagcctt gcagggtgcg ggtttcagcg
     5641 tggtacgcga aaccaaaaac agcctgagca ttgccgaccc ggacgggggg cggaacatcc
     5701 gattaaaggg ggtgctgtat gagcaagatt ttagacatgg cgaaggactt cgagcagagc
     5761 tcgaagcagc aggccaacgc tatcgagaag cgtctgaccg aagaattcac gaggcacgag
     5821 aaagctatct ccggggcgtt gaaatcaagc gaggagaaaa tgagcgccgc tattgccgag
     5881 cacagccaga aaatgggctg gctgttgctc aaaacgtgga tctggatgct ggtcagcatc
     5941 gtcctgttct tgagcgcaac gagcgggatc ctctggtatc agggggaatt gatagcggag
     6001 cggcaagcca ctctccaagc cttggcagag aagggcggca agctaaagct gacccagtgc
     6061 gggaagaaat tggagcacct ttgcgtgcag atctccgacc ccaatccaac cgtttggggg
     6121 aaggatacct attggatcgt g
//
