LOCUS       NZ_CP023944            10061 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_444 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP023944
VERSION     NZ_CP023944.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07312488
            Assembly: GCF_002587165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Croxen,M., Tallon,L.J., Sadzewicz,L., Ott,S., Zhao,X., Nagaraj,S.,
            Vavikolanu,K., Aluvathingal,J., Nadendla,S., Geyer,C. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Croxen,M., Tallon,L.J., Sadzewicz,L., Ott,S., Zhao,X., Nagaraj,S.,
            Vavikolanu,K., Aluvathingal,J., Nadendla,S., Geyer,C. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0, HGAP v. 3, SPAdes v. 3.6.0
            Genome Coverage                   :: 587.359x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 02:50:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,905
            CDSs (total)                      :: 5,783
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 78 of 171
            Pseudo Genes (incomplete)         :: 103 of 171
            Pseudo Genes (internal stop)      :: 16 of 171
            Pseudo Genes (multiple problems)  :: 26 of 171
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023944.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_444"
                     /isolation_source="Wound"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_444"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="Canada: BC"
                     /collection_date="2013-07"
                     /collected_by="BC Centre for Disease Control"
     gene            354..2318
                     /locus_tag="CRN70_RS30400"
                     /old_locus_tag="CRN70_30390"
     CDS             354..2318
                     /locus_tag="CRN70_RS30400"
                     /old_locus_tag="CRN70_30390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2318..3049
                     /gene="mobC"
                     /locus_tag="CRN70_RS30405"
                     /old_locus_tag="CRN70_30395"
     CDS             2318..3049
                     /gene="mobC"
                     /locus_tag="CRN70_RS30405"
                     /old_locus_tag="CRN70_30395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3056..3586
                     /locus_tag="CRN70_RS30410"
                     /old_locus_tag="CRN70_30400"
     CDS             3056..3586
                     /locus_tag="CRN70_RS30410"
                     /old_locus_tag="CRN70_30400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3613..3792)
                     /locus_tag="CRN70_RS30415"
                     /old_locus_tag="CRN70_30405"
     CDS             complement(3613..3792)
                     /locus_tag="CRN70_RS30415"
                     /old_locus_tag="CRN70_30405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3818..4246)
                     /locus_tag="CRN70_RS30420"
                     /old_locus_tag="CRN70_30410"
     CDS             complement(3818..4246)
                     /locus_tag="CRN70_RS30420"
                     /old_locus_tag="CRN70_30410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4410..4724)
                     /locus_tag="CRN70_RS30425"
                     /old_locus_tag="CRN70_30415"
     CDS             complement(4410..4724)
                     /locus_tag="CRN70_RS30425"
                     /old_locus_tag="CRN70_30415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4712..4990)
                     /locus_tag="CRN70_RS30430"
                     /old_locus_tag="CRN70_30420"
     CDS             complement(4712..4990)
                     /locus_tag="CRN70_RS30430"
                     /old_locus_tag="CRN70_30420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5165..5530)
                     /locus_tag="CRN70_RS31875"
                     /old_locus_tag="CRN70_30425"
     CDS             complement(5165..5530)
                     /locus_tag="CRN70_RS31875"
                     /old_locus_tag="CRN70_30425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5527..5898)
                     /gene="tolA"
                     /locus_tag="CRN70_RS30440"
                     /old_locus_tag="CRN70_30430"
     CDS             complement(5527..5898)
                     /gene="tolA"
                     /locus_tag="CRN70_RS30440"
                     /old_locus_tag="CRN70_30430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(6172..6417)
                     /locus_tag="CRN70_RS30445"
                     /old_locus_tag="CRN70_30435"
     CDS             complement(6172..6417)
                     /locus_tag="CRN70_RS30445"
                     /old_locus_tag="CRN70_30435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6744..8429
                     /locus_tag="CRN70_RS30450"
                     /old_locus_tag="CRN70_30440"
     CDS             6744..8429
                     /locus_tag="CRN70_RS30450"
                     /old_locus_tag="CRN70_30440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8439..8696
                     /locus_tag="CRN70_RS30455"
                     /old_locus_tag="CRN70_30445"
     CDS             8439..8696
                     /locus_tag="CRN70_RS30455"
                     /old_locus_tag="CRN70_30445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            8781..8930
                     /locus_tag="CRN70_RS30460"
                     /old_locus_tag="CRN70_30450"
     CDS             8781..8930
                     /locus_tag="CRN70_RS30460"
                     /old_locus_tag="CRN70_30450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1744..10061
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(9034..9613)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.113"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2319..3046
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 accacccaca aaaatccacg gtgctggacg cacctgacga agcagcaaag ctgctcctga
       61 ttttgcgggt ggtcagccgc tcgctatggc gaactctgac gggggccgga acaccggaaa
      121 agaccgcact ggggtgccgc cttttcctct cacacgctgg ggcgtgctcg ttgttcctcc
      181 ggggctgcgc cttttccgca tggggcggaa agaggctggc ctccgcggtg aaagctgaac
      241 gcaatcacca cggacgtgac cgccgccgcc atgccttggg gcatgtccgc ggcttgtgca
      301 gcgggggctg cgcatcacgc cggtaaaccc gtcgtctgaa ggagagagat tgcatgttta
      361 atacggattc gcttgcctgg cagtggcata tcccggagct ggcccggcag atttacccgt
      421 gggtcagctg gctgtaccgg gagtattaca gtctggccgg tttttttctg gttatcggcc
      481 tgtttttcgg tctggctgaa ctctgggccg gccgaatgcg ttttgtgggg ctgagccata
      541 aggcgcggcg ggagtatgcc gatcgctata agcgcgataa taaccggact gtgccatttt
      601 cggtgctggc ctcgctggat tcacccgggc ggatggtcag ggcacggcga catgtctgga
      661 actacgcgta ctggctgacc ggcgctgccg gtctggccct tctggggggg ctggttgtcg
      721 ccgtgtttat cgagagctgg ggagaaaccg tcctgcgccc ggacctctat gcgaacgcag
      781 attattacct gctgcagccg gctaacctgt tcgggatagc ctggtgtgcc ggagggcttg
      841 tcgccggcgt ccttctggcg cggctgctgg cgttcctgct gcttgatggt cattttgcgg
      901 ccgctgacga ggcggtgaac gcgaaactga atcaggagag ccggcgcagc agccagcgga
      961 ccggagaaat gacagacgtc cgccacctgc atttcgggga gccggtaccg gttaacgcgc
     1021 tggccgattt ttccacggag caggcccgga aacagcaggc ggtgtttctg ggtaaggatg
     1081 agcagggcca gccggtgctg gtccctcgtg atacctggcg caagaccaat attcagatcc
     1141 tcggcctgcc cgggagcggc aagagcgcga tggccaccaa cgcgctcatt cgctgcgtac
     1201 gcgatttcgg ggacgctgtg gtgtacttcg accccaaagg ggacgcgtgg gcgccgcacg
     1261 tcttccgggc gcactgcccg gacttcaccc tgctggatct gcggccgggg aaaccggcgc
     1321 agctgaatct gttccgcgat ctcgaccagt acgccctgaa gaatctgctg gttgccgggt
     1381 ttaacctgag tgagaccggc gacgtggcgg accactaccg catcagcgag cagaaagcgg
     1441 cgaagctgat tgcggagcag ttcccgcagg gggcaaacat tcagcaggtg ctggcggcgg
     1501 cgtacgcgtt gccggaggcc ctgaaaaagg acgtgaaggg gctgatcacc aagctggaga
     1561 acgtggccga cctgagcgtc ctgcagacgg acagcggtat cgatgtggcg gggataatca
     1621 acggcggcgg ctgcctgtac gtcatcggct cgatggacga tgaggcggtg atccgggtgc
     1681 agaagatgct gtttgcccgc tgcgcgcaaa tcatcattgc gcgggatgag ttccggaggt
     1741 ggccgcacgc gagcatcatg ctcgatgaga ttaaatatct gctgtcgaag tacgtgctga
     1801 acgcgctggg gacgctgcgc agccgggact gcaacctgct gctcgcgcac cagtcgctgg
     1861 gcgacttcgg tcagtgcggg caggatctgc ccgctgattt tgtgaaaacc acggtactgg
     1921 ataacacgcc gatccgctgg ttctatcgcg cggccagcca ggagtccgcg cagtgggcgg
     1981 cgggccagac cggggagatc cgggtggacg tggagcgccg ccgggccagc cgggaggcgg
     2041 ggaacgtgga gcacatcagc ggcgacagct tcatccagaa ggaggcgcgg ccgctgtttg
     2101 acgtgaacac cctgcaacac ctgcctgacg ggttcgcggt gatgaccggg ctgggcgtgg
     2161 cgcggctggc gttcagcagc ccgctgcggg ttgagcgccg ggaaatttcg ctgaaaacct
     2221 tcccggtgct ggcgaaaacg gacccgctgg cggagtacct gcctccggcc gacggcggcg
     2281 gaccaaaagc ggatgatgat tttgcggggc tttactgatg acactggagc agtacaacgt
     2341 cagccacgcg aagcggcagg cgcggaacgc ggagaaaaca cggctgaccc tgcgctggct
     2401 gcgggaagag ctgtgttcca cggcggagct ggttgcgcga cggctgggga ttgcggcggt
     2461 acagccggtt taccgcttcc tggacagtct ggtggcgaaa gggctgctgg tccgggcgaa
     2521 atacccggtt gacggccgtc aggtcagcgt gtgggggctg acgccgcacg gggtggcgtt
     2581 ctcgttcgac gaggacgagc cgctgacgga cgttattccg tttcagccgt caagggtgtc
     2641 ggcggcgcag ctgccgcacc ggctggccgt gcagtccctg cgcctctcca tggagggccg
     2701 gggggccacc ggctggcggt acctgcaccg tatggccctg aaagggatga aagtgccgga
     2761 cgcgctggcg gagctggacg gcagaacggt ggcatttgag gtagagcgca ccgtcaagag
     2821 ccgcaggcgg tatcaggaag tggtgtccgg gtatctgttc aaccgccggg cgaacggcat
     2881 cgatgaaatc tggtacatct gccctgaccg gccgacgctt gtccgggtac agcgggcgat
     2941 tctggcggtg gatgaaatcg tgaatccaca gaccggcgag gcccgcaaaa cggtggagct
     3001 ggaccgggag aggctgttcg cgtgctttaa atttatgaca acggagtgag ggattatggc
     3061 gctgtacatg gtatttattg aggacgtgga agacaaagac ggcgagcgct gcattatgcc
     3121 aaccagcgag catctggaag acatggtggt gctcgcctgg cagaccgggg cgtatgtttt
     3181 ctgttttgtc gaccgggccc agcatctggc gatgatgaaa tccatcggga tgtatgacga
     3241 gcggctcaac agcgacgaaa aagccacgta ctttttcacc ggtgaaaatc cgcaatggga
     3301 acgggacaaa cccacaggaa tgaagctgtg tgattacctc tatcgaaagg tggagcggtt
     3361 cgccatcgag aacggtgtgc aggtggttga tgccgggaag tgctcaccgg agcagattaa
     3421 cgcgatgatt gaggtggtca gcgagcggca cggggaaagc gcggtgaacg agaagctgaa
     3481 agcggagctg atgcgggaat ataacagccg ctattttaac gtgccggtgc ctgaactgcc
     3541 ggaggacaaa cccgcccgcc ggtggtggcc gttcggcaaa aaataacgcc agcaccggca
     3601 tggccggtgc tgttattctt ccccgagctt atcccgcatc acctgccaga gtgtttccgc
     3661 atcctcgtga agccgttcgc acatgtcggc cggggtatcc atgtccagct cgttcagctt
     3721 ttccagcaac agcagcgact gatccttaat gaatttagcc atattgaggg cagttttttc
     3781 ctgcttcgtc atgttctcca ttcccttcgg taagttctca gctaaaccag ccggaccaga
     3841 agcgtttttt cttctgtgtc agttctgttg tcaatctgtc cacttcggcc tggagtttcc
     3901 ggcgctcatc atcgtgctgg cggcgttctt cttcccggga tgttttatct tccagcatca
     3961 gggtgacagc ctgccggaga tcggccagtt ccttttgcat tgccagaagc tgttcctgtg
     4021 ggaaattttt ttgtgtattt tcacggtgtg aaatgccggg aatttgtttc tcagggatat
     4081 tcacatttcc gtatacacgg atcagttctg acacatccac gacagggttg tttttcccgt
     4141 cacgtgacac ggtaaccttt ccctgtttta catgattgta tagcgttctt ctggtaatgc
     4201 ccgcggcttg tgctgcctgt gaaaggttga gcaatgtttt cgccactgta tacccctgat
     4261 tttatcgggt gtgaaaggtg tgtaatttct cacggtaaat gtatcgctga attttacccg
     4321 tttttccgtt caggatgtgg ttttctgacg cgctgcgctt gtcaaaccgg cgacggccac
     4381 gcaatgaatt gcgcccccgt catcggttat tagaggctgt ctgcccccgg atacccggat
     4441 tctttctgat gcctgacggt gacgatcaca atctggtcca tttcccggtc gtggcggtag
     4501 agcatcacat agccactgtc gccaaacccg atcaccagtt ccttatattc cagcggcaga
     4561 aacggcaccg ggcggccaat gtctggcaat gttttcagtt gctggatagc ccggacgatc
     4621 acctcacctc cttttctggc tgccagccga tttttggttt tgagaaagtc ctgaaggcgt
     4681 tgtaaatcgc gctgtgccag cgcggaaatc gttacctgtg gcatggcgga gcatcctgct
     4741 cgttctcagt tccccaggta cttatccagg cttccgcttc ttccgcagta aggtgcagac
     4801 ccgtttcctg atagtgctgc catgcggcct gtccgtcccg caggtactgg ctgcgcttct
     4861 cttcccggtc gatgtattcg gtgattgcct ccagcattag cgcgtgcgct gagcggtgcc
     4921 ggtcatcggc cagcgttttc agccggtcct taagttcttc atcgagcctg atggatgttg
     4981 ctgatgacat aaaagcccct ttgtagtcat atgtattaca catgactact ttaatccgtg
     5041 gcggcgctat ttgccagaaa tacggctttt gctgcccatt tttcagcttc gcttccacta
     5101 atcagggctg ctacaggctc actcatcatc caggactatt gcagcctgcg ggttaaagga
     5161 cctgttactg gcggcaacca tcatcattga gcttttgggt ggtgactggt cgcggatcct
     5221 gtaagccgtc atttttcaga ctgaattcaa aatttataac ctgtcgcatt gccgggtgcc
     5281 ctgcgggata tagccagtgc ctcattgccg tcagcgtttc ccgaaaaaaa aggtgacagc
     5341 cggtaagcga cacgttgtcc actcgtccat cgtccagaat atcaaacgtg gcggtcacct
     5401 gaccttcgat gcgttttgct gcggcctgta ccgggtaggt tggctgcagt gtggttacgg
     5461 gttttagtgg tatcgcatgt accggagtga ggaacatggc cgccagcact gtcagagtaa
     5521 aagctttcat ggtctgaaat ccagaggggc gtttttgaat atttgccagg tattttcatc
     5581 cggtgccggc ggaattttgg cgtgggtaac ggctgatatt gctgctttac agagtttcgg
     5641 atctcctcct tcagcaatgg cgctgttcag agtgccatca cgttgtatgg atatccgcac
     5701 ggtgcacatt tttcccgcat aggcctctgg ttcaaacatc tgcatttcaa cagcggtctt
     5761 gatggccgca gcgtagctac tgattttaga tgacttatcc agagtataaa ttttcggcgg
     5821 ctgggtggtg gaatgcttac aggccgtcag gagtaagggg ctgcacaatg ccagcaggat
     5881 aaagggtatt aaacgcatcg ggttgatgac ctgtcagtaa aaaatgttac atgtatattt
     5941 gtcactcttt acggccacgt tcatttacat aaacacggct tatcgtatcg tttttacacc
     6001 aaacggcccg ccatgcagtt gcgtgcatag gcatgcagcg ggttacaatt taccgggcgc
     6061 gtttccggcg gttttccggg tggtttgttg cctgttttat cccgtagccg ccggaaacgc
     6121 cctcagaccg tctgagcggt gcgggcaatg cgacgttatg gtaaataccc cttaccggcg
     6181 gcgctgtggc tgccaggtga aggtggtttc cgtagcctcg tatttctccc gacgcatttc
     6241 ctgcgcccgc ctggcttcac gttcgtcctg aatggcacga agcacccttt cccgcacctg
     6301 ttgttttacc gcctttggca gcgttttgtt ccctttcagg cccatggcca gtgcctgagc
     6361 gacaaaggcg tccgccaggc gcttttcttc agcgggagtc agtttcaggt gcgtcatagt
     6421 ggcctccggg cgtctcaggt ttcccgaaca gtttaaccgc aggtcctctc ctcatgccag
     6481 ttagctttca gatgtgtcta taccatctga aatgtaagtg tgatgaatgt cttaaataat
     6541 aaaatttgtt gccatgagaa attttcggga aaagacgcga tctgcttaca ggtggctccg
     6601 ggcgcttttt tgtgcctgaa gatgagaact aatctcatat attgacacct gaaaacggga
     6661 ggagtaaggt aataattata ctgtgtatat gtacagtatg ttggctttaa tttattgttt
     6721 taaatgtcaa agaggaaaac ggtatgagtg gtggagacgg tcgaggtccg ggtaattcag
     6781 gtctgggaca taatggtggt caggccagtg ggaatgtgaa cggtacgtct ggtaaaggtg
     6841 gcccttcatc aggtggtggt acggatccaa acagcgggcc gggctggggt acgacgcata
     6901 cgccgaatgg agatattcat aactataatc cgggggagtt tggtaacggc gggagtaaac
     6961 ccggtggtaa tggtggtaac agcggcaatc atagcggtag ctctggtggc ggacagtctt
     7021 cggccactgc gatggccttc ggtctgcctg ccctggctac tccgggggct gaaggactgg
     7081 ctttatcagt ttccggcgat gcgttgtcgg ccgccgttgc tgatgtgctg gctgccctga
     7141 aggggccgtt taagtttggt ctgtggggga ttgcgatcta cggtgtgctg ccttctgaga
     7201 ttgcaaaaga tgatccgaaa atgatgtcaa aaattatgac gtcattaccg gccgatgcgg
     7261 tgacggagac tccggcaagt actttaccac tggaccaggc gacggttcgt gtcagacaac
     7321 gggttgtgga tgtggtgaag gatgagcggc agcatattgc ggttgtcgca ggtcggccaa
     7381 tgagtgtccc tgtggtggat gcgaaaccga caaaacgtcc gggggtattc agtgtgtcga
     7441 ttccgggtct cccgtctctg caggtgagcg taccgaaagg tgttccgaca gcgaaagccc
     7501 cgccaaaagg cattgttgct gaaaaaggtg attcacgtcc ggctggtttt acagccggtg
     7561 gtaactcccg tgaggccgtt attcgtttcc cgaaagagac cggacagaag ccggtttatg
     7621 tgtcggtgac agatgttctt accccggcac aggtaaaaca gcgtcaggag gaagaaaagc
     7681 gtcgccagca ggcatgggac gccgctcatc cggaagaggg gctgaaaaga gactatgata
     7741 aagcgaaagc tgagctggat gccgaagata aaaatattgc gaccttaaac agccgcattg
     7801 catcgacaga gaaggcgctc cccggtgcaa gggctgctgt tcaggaagcc gataaaaagg
     7861 taaaagaggc agaggcaaat aaagatgatt ttgtgactta taacccgcct catgaatatg
     7921 gctccgggtg gcaggatcag gttcgctatc ttgataagga tattcagaat cagaatgaga
     7981 aattaaaagc ggctcaggca tctttaaacg caatgaatga atccttatcc agagataagg
     8041 ctgcacttac cggggcgatg gagagccgga aacaaaagga gaaaaaagcg aaggatgcag
     8101 aaaataagtt aaatgaggaa aagaacaagc ctcgcaaggg gactaaagac tacggccatg
     8161 attatcatcc agtacctaaa acagaagaca taaagggact tggagagtta aagcgaggtg
     8221 atcctaaaac acctaaacaa ggtggtggtg gtaagcgggc ccgatggtat ggcgataaaa
     8281 aacgtaaaat ttatgaatgg gattcccagc acggtgagct tgaagggtat cgtgccagtg
     8341 atggcgaaca cctaggcgca tttgatccca aaacgggtaa gcaggttaaa gggccagatc
     8401 caaaacggaa cattaaaaaa tatctttgag aggtaaatat ggggcttaaa ttacgtttga
     8461 catggtttga taagaaaact gaagagttta aaggggaaga gtattctaaa gactttggtg
     8521 acgatggttc tgtcattgaa agcttgggaa tgcctttaaa ggataatatt aataatggtg
     8581 agtttgattt gaagaaagaa tgggttcctt tactacagcc attttttaaa aatcaaataa
     8641 aacctgatgt ggataattgt tatatagctt ttgattatcg ggacggtaac tggtaacaaa
     8701 aaattatcaa ggatgatttg aaatactgac ttccggtgtt cagggatgaa tgccggagtc
     8761 tttaaataca gggaaagtat atgaaaaagg taaagactat ttttcttttt atattgattg
     8821 ttgctggctt tcttctggcc gcatgtcagg caaattatat ccgggatgtt cagggcggaa
     8881 cggtggcacc atcgtcctcc tctgaactga cggggatcgc ggttcagtag aaaagatcaa
     8941 aggatcttct tgagatcctt tttttctgcg cgtaatcttt tgccctgtaa acgaaaaaac
     9001 cacctgggga ggtggtttga tcgaaggtta agtcagttgg ggaactgctt aaccgtggta
     9061 actggctttc gcagagcaca gcaaccaaat ctgtccttcc agtgtagccg gactttggcg
     9121 cacacttcaa gagcaaccgc gtgtttagct aaacaaatcc tctgcgaact cccagttacc
     9181 aatggctgct gccagtggcg ttttgcgtgc tttcccgggt tggactcaag tgaacagtta
     9241 ccggataagg cgcagcagtc gggctgaacg gggagttctt gcttacagcc cagcttggag
     9301 cgaacgacct acaccgagcc gagataccag ctgtgtgagc tatgagaaag cgccacactt
     9361 cccgcaaggg agaacggcgg acaggtatcc ggtaaacggc agggtcggaa caggagagcg
     9421 caagagggag cgacctaccg gaaacggtgg ggatctttaa gtcctgtcgg gtttcgcccg
     9481 tactgtcaga ttcatggttg agcctcacgg ctcccacaga tgcaccgaaa aagcgtctgt
     9541 ttatgtgaac tctggcagga gggcggagcc tatggaaaaa cgccaccggc gcggccctgc
     9601 cgttttgcct cacatgttag tcccctgctt atccacggaa tctgtgggta actttgtatg
     9661 tgtccgcagc gcccgccgca gtctcacgcc cggagcgtag cgaccgagtg agcgaggagg
     9721 cgcaatttcc ccggcacctg caagacggtc accggacgcg ctgatgtggg atattgtccc
     9781 ctgattaagt ctttctattc cctgacgatc aattatcagg ataggtgaac catgtcggtt
     9841 tttcgctgaa cattttacgt taacctatct ctgttgcttt tttctccttg cggagaaatg
     9901 caagtcatcc ccgctggggc gtggctgact tcaggggaaa agaccttgct tacgctggct
     9961 ggacgccacc gacgcacggt cttttcccct ctttctggct ctgtccggct ggacgccggc
    10021 cacgccagaa attcacccgg cccccgtcag gctcacgcct g
//
