LOCUS       NZ_CP023940             1556 bp    DNA     linear   CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_444 plasmid unnamed.
ACCESSION   NZ_CP023940
VERSION     NZ_CP023940.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07312488
            Assembly: GCF_002587165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Croxen,M., Tallon,L.J., Sadzewicz,L., Ott,S., Zhao,X., Nagaraj,S.,
            Vavikolanu,K., Aluvathingal,J., Nadendla,S., Geyer,C. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Croxen,M., Tallon,L.J., Sadzewicz,L., Ott,S., Zhao,X., Nagaraj,S.,
            Vavikolanu,K., Aluvathingal,J., Nadendla,S., Geyer,C. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0, HGAP v. 3, SPAdes v. 3.6.0
            Genome Coverage                   :: 587.359x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 02:50:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,905
            CDSs (total)                      :: 5,783
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 78 of 171
            Pseudo Genes (incomplete)         :: 103 of 171
            Pseudo Genes (internal stop)      :: 16 of 171
            Pseudo Genes (multiple problems)  :: 26 of 171
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023940.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_444"
                     /isolation_source="Wound"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_444"
                     /db_xref="taxon:573"
                     /plasmid="unnamed"
                     /geo_loc_name="Canada: BC"
                     /collection_date="2013-07"
                     /collected_by="BC Centre for Disease Control"
     gene            complement(224..625)
                     /locus_tag="CRN70_RS00005"
                     /old_locus_tag="CRN70_00005"
     CDS             complement(224..625)
                     /locus_tag="CRN70_RS00005"
                     /old_locus_tag="CRN70_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(625..816)
                     /locus_tag="CRN70_RS00010"
                     /old_locus_tag="CRN70_00010"
     CDS             complement(625..816)
                     /locus_tag="CRN70_RS00010"
                     /old_locus_tag="CRN70_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          938..1345
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 tctatgacga gtcgtcatag agtcggatct atataaataa aatttaaaaa acgaaatttc
       61 aggaaagtaa cccactgata ttaaatacaa atctgcaaaa tattaatttt gcgtggggtg
      121 tgggtatttt tagtggtgag gagcgttcag agatctgacg atttttggga aggttcagga
      181 actccccggg atggccggag aggaaaactg aagcatgacg gggtcaggat tttttccaga
      241 atttccacca tggcgccggg gattcagtta attttgcctg gtgattttcg agaaggagca
      301 tggcctgttt caggctgtca atgtgcgaat ccttggacaa aagtaaggta ttcaggtgtt
      361 caatctcctg tttcagatga cgaatcacct gttcactctg gtcagcagag gctgtatcac
      421 gctgttcaga gacctgtcca gaatggtgtt cacccttcgg ttgctcaaag ggaccaaaga
      481 cacgtaaaag ctctgaagta tctaccattt ttgcattgcg ttcaccgcta caggtggaca
      541 ggtcacctgc tttaatcaac ctgtgcagtg ttgttctact cttacccgtc agtcgggcag
      601 cctcactaat agacactctg gccatcagtt accatcctgt aattcatcca gtctcgagga
      661 cagtgtacgg aaaagatgct ccgcatcatc gtggagtttc tcacaaaggt cggcctcgac
      721 atcgagatca agctcgttaa gcttctccag caggagcaga gactgattct ggatgaattt
      781 agccatgttc agcgctgttt tctgttgttt cgtcataaga agcctcagag cgtcagtgct
      841 cagtgtaaga acagtctctt ttccgcttcc tcgctcactc agtcgctacg ctcctgccgt
      901 tcggctgcgg cgagcggcag gagcatgcgt agaaacgctc gagaaaaccg agtaatcgca
      961 tggaggaaag aaggaaaggc cgcacagcgg ccgtttttcc ataggctccg cccccctgac
     1021 gagcatcacg gaatctgacg ctcaaatcag tggcggcgaa acccgacagg actataaaga
     1081 taccaggcgt ttccccctga tggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     1141 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
     1201 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
     1261 ctgcgcctta tccggtaact atcatcttga gtccaacccg gaaagacacg caaaagcgcc
     1321 actggcagca gccactggta actgatttag aggagttagt cttgaagtta tgcgccagtt
     1381 aaggctaaac tgaaagaaca agttttggtg actgcgctcc tctaagccag ttaccttggt
     1441 tcatagagcc gcaaactcta gaaccttaaa aaaaccgccc cgcaaaggcg gtttttttct
     1501 ttctcgtctc aaggttaagc tctgtataac atcagagtca acagatcttc atttcg
//
