LOCUS       NZ_CP023902             2101 bp    DNA     circular CON 14-JUN-2024
DEFINITION  Escherichia coli strain FDAARGOS_433 plasmid unnamed9, complete
            sequence.
ACCESSION   NZ_CP023902
VERSION     NZ_CP023902.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07312477
            Assembly: GCF_002589795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Croxen,M., Tallon,L., Sadzewicz,L., Ott,S., Zhao,X., Nagaraj,S.,
            Vavikolanu,K., Aluvathingal,J., Nadendla,S., Geyer,C. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Croxen,M., Tallon,L., Sadzewicz,L., Ott,S., Zhao,X., Nagaraj,S.,
            Vavikolanu,K., Aluvathingal,J., Nadendla,S., Geyer,C. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0, HGAP v. 3, SPAdes v. 3.6.0
            Genome Coverage                   :: 696.335x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002589795.1-RS_2024_06_14
            Annotation Date                   :: 06/14/2024 04:40:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,097
            CDSs (total)                      :: 4,972
            Genes (coding)                    :: 4,693
            CDSs (with protein)               :: 4,693
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 279
            CDSs (without protein)            :: 279
            Pseudo Genes (ambiguous residues) :: 0 of 279
            Pseudo Genes (frameshifted)       :: 87 of 279
            Pseudo Genes (incomplete)         :: 190 of 279
            Pseudo Genes (internal stop)      :: 46 of 279
            Pseudo Genes (multiple problems)  :: 39 of 279
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023902.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_433"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_433"
                     /db_xref="taxon:562"
                     /plasmid="unnamed9"
                     /geo_loc_name="Canada: BC"
                     /collection_date="2015-12"
                     /collected_by="BC Centre for Disease Control"
     gene            complement(join(1141..2101,1..5))
                     /locus_tag="CRN16_RS26080"
                     /old_locus_tag="CRN16_26075"
     CDS             complement(join(1141..2101,1..5))
                     /locus_tag="CRN16_RS26080"
                     /old_locus_tag="CRN16_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            complement(871..1137)
                     /locus_tag="CRN16_RS26075"
                     /old_locus_tag="CRN16_26070"
     CDS             complement(871..1137)
                     /locus_tag="CRN16_RS26075"
                     /old_locus_tag="CRN16_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          complement(1482..1713)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
       61 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
      121 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
      181 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
      241 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
      301 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
      361 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
      421 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
      481 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
      541 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
      601 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
      661 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
      721 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
      781 gctgaggata atgcaggata gacagagcac tagagattgc atgcaatctc gtacctatca
      841 cctcagcctg ccgctggctc ttttccagaa ctaagaatgc gttcttaaaa atccaaactt
      901 gataccgttt aaatctattt tcacaaaaag agtttttacc ctctcgtttg ttttctgaat
      961 atataaaatt aattcatcct caaaaccttc gtgcggatga aaaccaaatg tgccaaaact
     1021 taatccatca ataagcaaaa taaggttttt aaacctgtca ggaatcaccg cttcacttct
     1081 taaaaaaaat aaaccaatag tcttatttcc attcaggtat tcaatattaa tattcatacg
     1141 ttaatccgat ttatccttct caggggcgcg tttgtaacgc cgtttacctg aatcccagac
     1201 aaacgccgtc cgcttcccgt catcagtgcc atcccctaca tcgtcggcaa tgacaagatc
     1261 ctcattggtt tctcgatcca actggaggac gttttttagc gccccaccgg tcgagataaa
     1321 acggcgcttg tgaagctgcc gcgtcagctc aagaaaccac tcaggatcgt ttgccatatc
     1381 ttccggttta acggagtatt tcagcgtttc agcaaccgcg ctttgcagtt gctcggcaac
     1441 gttggccaca acctcacctg tcttagtttt tactgcccga atatcgatat tcggctcata
     1501 gttcacccgc aagcaatcgc gccagagttc tacccaacgt tcgtgcttaa cgtagttctt
     1561 ccctttaaac caagaaggtt gcaccatcag cagacagtga aaatgcggat gtgcgctgcc
     1621 atccttacct cgcgtcacct ccgtagccct gatccacccc tgaacaggtg atagctcctt
     1681 tcgcttttcc attcgcttaa acgccgcatt cattgctgta aggactgttc caagttcacc
     1741 tatctcgcag ttcctgacag ttaacgtcag aaacaaccat cgggaagacg ggtaatccac
     1801 aacgattttc ggtagagcct gataaaaacg ggcttgccac atgagggttc ttctccactg
     1861 gcagacaggg caatgacgga cacggcaaaa ttgcgcacta cgaagccgta ggcgcgtttc
     1921 tccggtttcc atgatggtag accaaccaaa acgtaacaaa ccgctacagg aggccatacg
     1981 gagtgcatag cgctcgaatt cagcagcagt ctggtagata cccccgacag aatcggtaag
     2041 cgtgcgctgg gtatcccaaa ctgcatcacg ggggctgtag tccgaaagga atttatcttc
     2101 t
//
