LOCUS       NZ_CP023901             1552 bp    DNA     circular CON 14-JUN-2024
DEFINITION  Escherichia coli strain FDAARGOS_433 plasmid unnamed10, complete
            sequence.
ACCESSION   NZ_CP023901
VERSION     NZ_CP023901.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07312477
            Assembly: GCF_002589795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Croxen,M., Tallon,L., Sadzewicz,L., Ott,S., Zhao,X., Nagaraj,S.,
            Vavikolanu,K., Aluvathingal,J., Nadendla,S., Geyer,C. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Croxen,M., Tallon,L., Sadzewicz,L., Ott,S., Zhao,X., Nagaraj,S.,
            Vavikolanu,K., Aluvathingal,J., Nadendla,S., Geyer,C. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0, HGAP v. 3, SPAdes v. 3.6.0
            Genome Coverage                   :: 696.335x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002589795.1-RS_2024_06_14
            Annotation Date                   :: 06/14/2024 04:40:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,097
            CDSs (total)                      :: 4,972
            Genes (coding)                    :: 4,693
            CDSs (with protein)               :: 4,693
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 279
            CDSs (without protein)            :: 279
            Pseudo Genes (ambiguous residues) :: 0 of 279
            Pseudo Genes (frameshifted)       :: 87 of 279
            Pseudo Genes (incomplete)         :: 190 of 279
            Pseudo Genes (internal stop)      :: 46 of 279
            Pseudo Genes (multiple problems)  :: 39 of 279
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023901.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_433"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_433"
                     /db_xref="taxon:562"
                     /plasmid="unnamed10"
                     /geo_loc_name="Canada: BC"
                     /collection_date="2015-12"
                     /collected_by="BC Centre for Disease Control"
     gene            join(1107..1552,1..31)
                     /locus_tag="CRN16_RS26065"
                     /old_locus_tag="CRN16_26060"
     CDS             join(1107..1552,1..31)
                     /locus_tag="CRN16_RS26065"
                     /old_locus_tag="CRN16_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          625..710
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.954"
                     /coverage="95.0"
                     /evalue="4.39e-26"
     Typing          complement(920..1028)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.091"
                     /coverage="100.0"
                     /evalue="9.09e-49"
     Typing          complement(262..522)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.511"
                     /coverage="100.0"
                     /evalue="3.95e-106"
ORIGIN
        1 gcgccgaatc atggccatgt tttcaaattg agccagcggc aggcggacaa tcaggggcta
       61 cgtgttaacg ttctgaccat gattgtctat cctgcattgc tcttttgccg cctcaaaatc
      121 ccttgcgtgt ttttgctccc cgatctccag aacgaccgag ccgccacggt tccggcagcg
      181 ccttgagcgc aaccggaaac cgtctgggga gaaccccaga cccccatcgc tgtaacaccg
      241 acggtctacg accacctacc aaccgagtga ctgcctgtac cgtgcaaatg ggctttgcag
      301 ggggctggcg cggttcctgg cgacctgaaa gcccctggct aggggtatcg gcgcgcctga
      361 cctcctgaaa cgctctgtaa gcgtttttcg cgccttccat gccctgacgg gcatttaaac
      421 cccgcctcgt gcgtctgaga gcttctgagc gcgtttgaag ggctatctga cgactcatcc
      481 ccgaaagaac aacagcggaa tcaccgacaa catgcgagag gtggcaccgc tcgccgcagc
      541 cccagcgacc gaacgttagt gagcgagtgg gcgaggaagc ggaagagaac cggaaaccac
      601 attgagcacg tacgcactga tacggggtgt cggggcgaag ccctgaccag atggcaaacg
      661 gaatatcgtc gcgggtgacg gtattacatt tgcacatcct gtcccgattt ctgcgggggt
      721 taacggtgaa cggacggcaa aaaacgtgca ggagctgttg aacgtgaaaa ccacgaaatc
      781 tggcaaaaat cacggctggc gaggcttgca gaggagtaag ccagtataca ctccgctatc
      841 gctacgtgac tgggtcaggg cttcgccccg acacccccaa agggcgttgc gttgcgcgca
      901 acacccttgc ctagaataga tcttttacgt cgatttgtaa gttgttgttt ttttagtgtg
      961 ttttttgttt acctgtcgca tccagggtga cacaacgttg tcgcatcctg gatgcgacaa
     1021 cacgcaacaa ggtgttgcac agatgtctca tgatggctta gatttgaacc aacgttagtg
     1081 catgggattt ttagagggaa aaaatcatgt ttattgattc agaaaaacga ctgaaacaac
     1141 tttcagatga ggcaaagaaa aacaccgagg atctcgaaga agcaaagaag aattcaaggt
     1201 ttacacaggt atccccaaaa ggttgggaac gcgttcgaga gctgctgaag gatagccaag
     1261 gcatatcagc actgaagctg tactcatttt tagcggagca tatcgatcct acgtgtggcg
     1321 ctgtcgttgc ggatcagcaa ttcctagctg aaaaacttgg agttagcaga agcacaatta
     1381 ttcgttggct caattactta gaatcaaaaa atgcattagt tagaatcccc gttgctggta
     1441 aggtttgtgc gtatgccctc gatccacatg aagtctggaa gggatacaac actacgaaaa
     1501 accatgcagc atttgtcact aaaacactgg tcaacaaaga cggtgatatt ca
//
