LOCUS       NZ_CP023858             1552 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain 2/0 plasmid p2_0.5, complete sequence.
ACCESSION   NZ_CP023858
VERSION     NZ_CP023858.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07760925
            Assembly: GCF_003856635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Sandegren,L.
  TITLE     Dynamics of resistance plasmids in extended spectrum
            beta-lactamase-producing Enterobacteriaceae in the gut microflora of
            patients during 1 year post UTI
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Sandegren,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2017) Medical Biochemistry and Microbiology,
            Uppsala University, Husargatan 3, Uppsala SE-75123, Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics workbench v. 10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 17x
            Sequencing Technology             :: Pacific bio + Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003856635.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 02:27:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,253
            CDSs (total)                      :: 5,139
            Genes (coding)                    :: 4,906
            CDSs (with protein)               :: 4,906
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 84 of 233
            Pseudo Genes (incomplete)         :: 163 of 233
            Pseudo Genes (internal stop)      :: 48 of 233
            Pseudo Genes (multiple problems)  :: 53 of 233
            Pseudo Genes (short protein)      :: 1 of 233
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023858.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2/0"
                     /isolation_source="UTI"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2_0.5"
                     /geo_loc_name="Sweden"
                     /collection_date="2009"
     gene            complement(492..968)
                     /locus_tag="CRT29_RS26725"
                     /old_locus_tag="CRT29_27765"
     CDS             complement(492..968)
                     /locus_tag="CRT29_RS26725"
                     /old_locus_tag="CRT29_27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1048..1156
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          complement(1359..1451)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.426"
                     /coverage="100.0"
                     /evalue="4.39e-26"
     Typing          2..262
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.511"
                     /coverage="100.0"
                     /evalue="3.95e-106"
ORIGIN
        1 acctctcgca tgttgtcggt gattccgctg ttgttctttc ggggatgagt cgtcagatag
       61 cccttcaaac gcgctcagaa gctctcagac gcacgaggcg gggtttaaat gcccgtcagg
      121 gcatggaagg cgcgaaaaac gcttacagag cgtttcagga ggtcaggcgc gccgataccc
      181 ctagccaggg gctttcaggt cgccaggaac cgcgccagcc ccctgcaaag cccatttgca
      241 cggtacaggc agtcactcgg ttggtaggtg gtcgtagacc gtcggtgtta cagcgatggg
      301 ggtctggggt tctccccaga cggtttccgg ttgcgctcaa ggcgctgccg gaaccgtggc
      361 ggctcggtcg ttctggagat cggggagcaa aaacacgcaa gggattttga ggcggcaaaa
      421 gagcaatgca ggatagacaa tcatggtcag aacgttaaca cgtagcccct gattgtccgc
      481 ctgccgctgg ctcaatttga aaacatggcc atgattcggc gctgaatatc accgtctttg
      541 ttgaccagtg ttttagtgac aaatgctgca tggtttttcg tagtgttgta tcccttccag
      601 acttcatgtg gatcgagggc atacgcacaa accttaccag caacggggat tctaactaat
      661 gcattttttg attctaagta attgagccaa cgaataattg tgcttctgct aactccaagt
      721 ttttcagcta ggaattgctg atccgcaacg acagcgccac acgtaggatc gatatgctcc
      781 gctaaaaatg agtacagctt cagtgctgat atgccttggc tatccttcag cagctctcga
      841 acgcgttccc aaccttttgg ggatacctgt gtaaaccttg aattcttctt tgcttcttcg
      901 agatcctcgg tgtttttctt tgcctcatct gaaagttgtt tcagtcgttt ttctgaatca
      961 ataaacatga ttttttccct ctaaaaatcc catgcactaa cgttggttca aatctaagcc
     1021 atcatgacac atctgtgcaa caccttgttg cgtgttgtcg catccaggat gcgacaacgt
     1081 tgtgtcaccc tggatgcgac aggtaaacaa aaaacacact aaaaaaacaa caacttacaa
     1141 atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg caacgcaacg ccctttgggg
     1201 gtgtcggggc gaagccctga cccagtcacg tagcgatagc ggagtgtata ctggcttact
     1261 cctctgcaag cctcgccagc cgtgattttt gccagatttc gtggttttca cgttcaacag
     1321 ctcctgcacg ttttttgccg tccgttcacc gttaaccccc gcagaaatcg ggacaggatg
     1381 tgcaaatgta ataccgtcac ccgcgacgat attccgtttg ccatctggtc agggcttcgc
     1441 cccgacaccc cgtatcagtg cgtacgtgct caatgtggtt tccggttctc ttccgcttcc
     1501 tcgcccactc gctcactaac gttcggtcgc tggggctgcg gcgagcggtg cc
//
