LOCUS       NZ_CP023857             2101 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain 2/0 plasmid p2_0.4, complete sequence.
ACCESSION   NZ_CP023857
VERSION     NZ_CP023857.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07760925
            Assembly: GCF_003856635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Sandegren,L.
  TITLE     Dynamics of resistance plasmids in extended spectrum
            beta-lactamase-producing Enterobacteriaceae in the gut microflora of
            patients during 1 year post UTI
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Sandegren,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2017) Medical Biochemistry and Microbiology,
            Uppsala University, Husargatan 3, Uppsala SE-75123, Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics workbench v. 10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 17x
            Sequencing Technology             :: Pacific bio + Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003856635.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 02:27:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,253
            CDSs (total)                      :: 5,139
            Genes (coding)                    :: 4,906
            CDSs (with protein)               :: 4,906
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 84 of 233
            Pseudo Genes (incomplete)         :: 163 of 233
            Pseudo Genes (internal stop)      :: 48 of 233
            Pseudo Genes (multiple problems)  :: 53 of 233
            Pseudo Genes (short protein)      :: 1 of 233
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023857.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2/0"
                     /isolation_source="UTI"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2_0.4"
                     /geo_loc_name="Sweden"
                     /collection_date="2009"
     gene            complement(join(2026..2101,1..890))
                     /locus_tag="CRT29_RS26700"
                     /old_locus_tag="CRT29_27745"
     CDS             complement(join(2026..2101,1..890))
                     /locus_tag="CRT29_RS26700"
                     /old_locus_tag="CRT29_27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            complement(1756..2022)
                     /locus_tag="CRT29_RS26710"
                     /old_locus_tag="CRT29_27755"
     CDS             complement(1756..2022)
                     /locus_tag="CRT29_RS26710"
                     /old_locus_tag="CRT29_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          complement(266..497)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ccgtcatcag tgccatcccc tacatcgtcg gcaatgacaa gatcctcatt ggtttctcga
       61 tccaactgga ggacgttttt tagcgcccca ccggtcgaga taaaacggcg cttgtgaagc
      121 tgccgcgtca gctcaagaaa ccactcagga tcgtttgcca tatcttccgg tttaacggag
      181 tatttcagcg tttcagcaac cgcgctttgc agttgctcgg caacgttggc cacaacctca
      241 cctgtcttag tttttactgc ccgaatatcg atattcggct catagttcac ccgcaagcaa
      301 tcgcgccaga gttctaccca acgttcgtgc ttaacgtagt tcttcccttt aaaccaagaa
      361 ggttgcacca tcagcagaca gtgaaaatgc ggatgtgcgc tgccatcctt acctcgcgtc
      421 acctccgtag ccctgatcca cccctgaaca ggtgatagct cctttcgctt ttccattcgc
      481 ttaaacgccg cattcattgc tgtaaggact gttccaagtt cacctatctc gcagttcctg
      541 acagttaacg tcagaaacaa ccatcgggaa gacgggtaat ccacaacgat tttcggtaga
      601 gcctgataaa aacgggcttg ccacatgagg gttcttctcc actggcagac agggcaatga
      661 cggacacggc aaaattgcgc actacgaagc cgtaggcgcg tttctccggt ttccatgatg
      721 gtagaccaac caaaacgtaa caaaccgcta caggaggcca tacggagtgc atagcgctcg
      781 aattcagcag cagtctggta gatacccccg acagaatcgg taagcgtgcg ctgggtatcc
      841 caaactgcat cacgggggct gtagtccgaa aggaatttat cttctgacat taagcctgct
      901 ccgatctggg caggctgtga tttgatgtgg tatattcatc acgtacctgt gcattgcgag
      961 ccagtacatg tcaccagagt ttgccgctga ggtgacacat tcgaaatgtt ggggagtgtt
     1021 gacgcactcc ccaacgccga ttaaaaacgc ctcaattctg ctccattttc tttgacctgt
     1081 caaaacccac tcaaagcctc gtcattactg ggctaaacca ctatttcata tccgccattt
     1141 atcccttaag tataaataca aggatcacgc gcgagaattg cgcaaaaaat aaccacaatt
     1201 ttaacccccc aaaacaagca cccccgaacg gcttacccca accttttcca cacacccgat
     1261 caccaaaaca gggaataaaa acaaaaccct gtcttacagg tggatggcct cacttcgtga
     1321 aaccatccac cccgcataag ttttttaatc ggtggtgtcg tcaatcacta aatcggaacc
     1381 ccaaagggga cccccacgat ttagcatttg acgagattaa ctctcggaga tggcatcatg
     1441 cttttaacgg actttaaaaa acggtgaacg gatgcggatt ttaatccgtg tctgtgaggc
     1501 gttgagcaac gagcaaagcg aggcggtaga aagagagcca gcggcaggct caggacgata
     1561 atccggggaa aatgtaccac tcgcagagtg ttaacatttg acatcggatt gtctatcctg
     1621 cattggtgtc gagttggcaa ggctgaggat aatgctgagg ataatgctga ggataatgca
     1681 ggatagacag agcactagag attgcatgca atctcgtacc tatcacctca gtctgccgct
     1741 ggctcttttc cagaactaag aatgcgttct taaaaatcca aacttgatac cgtttaaatc
     1801 tattttcaca aaaagagttt ttaccctctc gtttgttttc tgaatatata aaattaattc
     1861 atcctcaaaa ccttcgtgcg gatgaaaacc aaatgtgcca aaacttaatc catcaataag
     1921 caaaataagg tttttaaacc tgtcaggaat cactgcttca cttcttaaaa aaaataaacc
     1981 aatagtctta tttccattca ggtattcaat attaatattc atacgttaat ccgatttatc
     2041 cttctcaggg gcgcgtttgt aacgccgttt acctgaatcc cagacaaacg ccgtccgctt
     2101 c
//
