LOCUS       NZ_CP023856             2331 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain 2/0 plasmid p2_0.3, complete sequence.
ACCESSION   NZ_CP023856
VERSION     NZ_CP023856.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07760925
            Assembly: GCF_003856635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Sandegren,L.
  TITLE     Dynamics of resistance plasmids in extended spectrum
            beta-lactamase-producing Enterobacteriaceae in the gut microflora of
            patients during 1 year post UTI
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Sandegren,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2017) Medical Biochemistry and Microbiology,
            Uppsala University, Husargatan 3, Uppsala SE-75123, Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics workbench v. 10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 17x
            Sequencing Technology             :: Pacific bio + Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003856635.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 02:27:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,253
            CDSs (total)                      :: 5,139
            Genes (coding)                    :: 4,906
            CDSs (with protein)               :: 4,906
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 84 of 233
            Pseudo Genes (incomplete)         :: 163 of 233
            Pseudo Genes (internal stop)      :: 48 of 233
            Pseudo Genes (multiple problems)  :: 53 of 233
            Pseudo Genes (short protein)      :: 1 of 233
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023856.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2/0"
                     /isolation_source="UTI"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2_0.3"
                     /geo_loc_name="Sweden"
                     /collection_date="2009"
     gene            complement(988..1998)
                     /locus_tag="CRT29_RS26695"
                     /old_locus_tag="CRT29_27740"
     CDS             complement(988..1998)
                     /locus_tag="CRT29_RS26695"
                     /old_locus_tag="CRT29_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859061.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_059260076.1"
                     /translation="MKNPDSPILSLRDYSTQDSKWDSDRARADQVAKIYASDQQFSRRG
                     ERMFDCSQRLQFAPQSSRLTGEMRLALRHGEFCHVPFCPVCSRRRSLRWMRRLWEALPK
                     LLAENPTARWLFLTLTVKNPPVGELRETLKQMNAAWERLTKRKEFAPVLGWLRSTEITY
                     GNVLGGCHPHFHVVLWVPSSWFKRDYVKHERWVEIWSECLRVDYAAGAHIKRVRPKRGS
                     VPEGASAAEVQRAALEAGVIETLKYTVKSSEIVRDPAWFLELARQTYGLRMIATGGRFR
                     EILQVEKPETDEDLIGADMPAKPDEFEEMAFWLAFDWRRDEKRYKRNPRADRRKD"
                     /name="protein rep"
     Typing          252..339
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.506"
                     /coverage="96.0"
                     /evalue="1.21e-31"
     Typing          complement(672..751)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.902"
                     /coverage="96.0"
                     /evalue="4.39e-26"
ORIGIN
        1 accccaccca cttacaccca tctcacccac agactttcac cccgattttg tgaacaacaa
       61 aaaaccgtct tgcaggtggt tggcctcact ccgtgacacc aaccaccatc cccagcgttt
      121 tcccagtgcc gtcgtcaatc acaaaatcgg aacccacgcc agaggcgcgg ggccccgcga
      181 ttttctgttt gacgagttcg ggaacggtga gcggtacgcg gaattttttc gcgggccgtg
      241 aggcgttggc ggggtgtcgg ggcgcagccc tgaccagatg gcaattgtga tagcgtcgcg
      301 tgcgacggta ttacaattgc acatcctgtc ccgatttttt cgccagaacg ctggcagagc
      361 atcggcagtt ctgcgccgtt aacccctccg caggagcgca cagtttcccg acgaaggaga
      421 gaaactacta agtgcgcatc cgcacttaac gcgcggatga tttttggcac cgcagccacc
      481 acagctacgc aaaaatttcg gcgaccggac agcaaatcat tttgccgttt tcgtgacgca
      541 gccgttccgc gcaatcgaca aactggcagt acagaaaact cacgaagtgc gcacttatac
      601 aaacttcact tcgtttcgtt tgtcgtgcgg tctgcgacgc tcaaaaaaac cgagaaacgg
      661 agggaatttt cgggacagga tgtgcaattg taataccgtc gcacgcgacg ctatcacaat
      721 tgcccctggt cagggctgcg ccccgacacc ctcgacaccg taccagagac acgttaaaac
      781 gacatacgcg cgtttttggc aatcaacaga ccaaggggat ggtttgcgta gcatcaagcg
      841 ccagaaacgc gctcaaaacg cttctgagcg ccttaaaacg aaaaaacggg caaatccttg
      901 cctgtttctg aaaatcacgt tagaacactt ctgagcgcgt ttcacctcca ctgccgcacc
      961 atcccactat gcgcctcaga aacgcgctca atccttccgc ctgtctgctc tgggattccg
     1021 cttgtagcgt ttctcgtccc gcctccagtc gaacgccagc cagaacgcca tctcctcgaa
     1081 ctcgtccggc ttcgctggca tgtccgcacc aatcaaatcc tcgtccgtct ccggcttctc
     1141 gacctggagg atctcccgaa accgtcctcc tgtggcaatc atccgcagcc cgtaggtctg
     1201 ccgcgcaagc tccagaaacc acgccggatc gcgcacaatt tccgaggatt tcaccgtgta
     1261 tttcagtgtc tcgatcaccc ccgcttccag cgccgcccgt tgcacctctg cagcgcttgc
     1321 tccttccggt acactcccgc gttttggccg cacgcgcttg atgtgcgccc ccgctgcata
     1381 atccacccgc aggcactcgc tccagatttc cacccagcgt tcatgcttca cgtaatcccg
     1441 cttgaaccac gacgaaggaa cccagagcac cacgtgaaag tggggatgac agccccctag
     1501 tacattcccg taggtgattt ccgtactccg cagccagccc agaacaggcg caaattcttt
     1561 acgcttggtt aaacgctccc aagccgcatt catctgcttg agcgtctctc tcaactcacc
     1621 aacggggggg ttttttacgg tcagggtcag aaagagccat ctcgcggtag ggttctcagc
     1681 cagtagtttc ggtagcgcct cccacaaacg acgcatccag cgtaacgagc ggcgacggct
     1741 gcacaccgga cagaagggga catgacagaa ttcaccatga cgcagcgcaa gccgcatctc
     1801 gcctgtaaga cgggaacttt gcggagcgaa ctgtaaacgc tgactgcaat caaacatcct
     1861 ctcgccgcga cgggaaaact gctggtctga cgcgtaaatc tttgccacct ggtcagccct
     1921 cgcgcgatcg ctgtcccatt ttgaatcctg cgtactgtag tcacgcagtg agaggatcgg
     1981 actatctgga tttttcataa cccgccccca gtgggcaggg gcagaaatct tgtgttagca
     2041 tgttcttacc acctgtgttg atctcatcga attttacctt tcaggtggtc acccccgggg
     2101 tgttagcgca ctgccgggga acctcaccaa cctgttgctc acacggtagc caaaaagccc
     2161 cgcagacgca atcaaaacat ccacaaacca aaaccccctc aaacccgcgc cagcactggg
     2221 ctgaacaggg aaaaccaatc ctcgagttgt tgcctatagt ataaatacaa ggatcgcgcg
     2281 cgagatttgc ttgaattttg agcgggattt tcagtaaccc aaaacctccc a
//
