LOCUS       NZ_CP023855             4063 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain 2/0 plasmid p2_0.2, complete sequence.
ACCESSION   NZ_CP023855
VERSION     NZ_CP023855.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07760925
            Assembly: GCF_003856635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Sandegren,L.
  TITLE     Dynamics of resistance plasmids in extended spectrum
            beta-lactamase-producing Enterobacteriaceae in the gut microflora of
            patients during 1 year post UTI
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Sandegren,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2017) Medical Biochemistry and Microbiology,
            Uppsala University, Husargatan 3, Uppsala SE-75123, Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics workbench v. 10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 17x
            Sequencing Technology             :: Pacific bio + Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003856635.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 02:27:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,253
            CDSs (total)                      :: 5,139
            Genes (coding)                    :: 4,906
            CDSs (with protein)               :: 4,906
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 84 of 233
            Pseudo Genes (incomplete)         :: 163 of 233
            Pseudo Genes (internal stop)      :: 48 of 233
            Pseudo Genes (multiple problems)  :: 53 of 233
            Pseudo Genes (short protein)      :: 1 of 233
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023855.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2/0"
                     /isolation_source="UTI"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2_0.2"
                     /geo_loc_name="Sweden"
                     /collection_date="2009"
     gene            complement(join(2497..4063,1..581))
                     /gene="mobQ"
                     /locus_tag="CRT29_RS26670"
                     /old_locus_tag="CRT29_27715"
     CDS             complement(join(2497..4063,1..581))
                     /gene="mobQ"
                     /locus_tag="CRT29_RS26670"
                     /old_locus_tag="CRT29_27715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            760..1014
                     /gene="mobC"
                     /locus_tag="CRT29_RS26675"
                     /old_locus_tag="CRT29_27720"
     CDS             760..1014
                     /gene="mobC"
                     /locus_tag="CRT29_RS26675"
                     /old_locus_tag="CRT29_27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            1548..2249
                     /locus_tag="CRT29_RS26685"
                     /old_locus_tag="CRT29_27730"
     CDS             1548..2249
                     /locus_tag="CRT29_RS26685"
                     /old_locus_tag="CRT29_27730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(224..582)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          583..759
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1549..2249
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..668)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.297"
                     /coverage="99.0"
                     /evalue="8e-141"
     Typing          complement(2501..4062)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccctgtcgct cgtatgaacg atggtctatc tcttcctgat aaccggcgtt cgccagtgcc
       61 ctgtttgcat ggtcagccca tgatgcccgc cacgtctcgg catgtgtccg gtcgttccag
      121 tccctgacct ttccgctgaa cccagcaggt ccgatagccc tcgtggtcag catgatgtgc
      181 gcatggggat ttgttttatc catgtgatgg aacgccacat cagcaatcat gccctgactg
      241 acaaagtttt ctctgacaaa ggcgagaacg gtttcccgtg cggcatcctg cggcagttcg
      301 tgggggatgg cgagttcaat ttcccgcgca agttgtgagt tcttccggcg ttcggccttc
      361 tcaacggcat tccagagttc ggcacggttc gtacaccagg caggggcggt ggcgggggtc
      421 agaatcacag cctcagccac accgcttttg cgggtgtagt cgtgagtcag tccggtacgc
      481 tcatcaagca tcagtgaacc ggaacgatag gcagcggaag ctacggcgct gtagccgctg
      541 cttctggaga taattttcat gctgagatga taaatcgcca tttctgccgt aatgcctttt
      601 tgtagttttc tttcagcccc gcagggcgca cacaatcgaa acggagtgga gattgtataa
      661 gtgcgcactt cgtgatttgc cgtgcattat gccagctcag tttttaaact accagtgacg
      721 cgctaaaatg cgatttttca tcgcgcacgg agttacatca tgagcagaac gctcgaacag
      781 aagattgctg atgctgaggc gagattacag cgcctgaagg cgaagagccg gagcctggac
      841 acagcgcaga aggttgttgt gggtgctgca ttgctggcaa aggtcagaaa gccggaggag
      901 gtgcagttgc gtgcctggtt actccagttc ctgaaggcag aggtgacacg acaggctgat
      961 gtgacgcgca tactgccgct gattaacgag ctggaggcgc tgccagaaca gtgattgtat
     1021 cgtccgctcg ccgcagcccc agcgaccgag cgttagcgag cgagtgggcg aggaagcgga
     1081 agtgaatcag tcgccaggtt gagcacatac gcacggtttt tcggcaggtg atatgcgctg
     1141 aacccgcatg aatactgggt ttccacaggg ttggaggggg tgtattcgaa acttgcaatt
     1201 ctgctttatt gcaacgcatt gtttttattg gttttttgtt ttcagcaccc caagttatcc
     1261 acaggctcgc cgttaagttt ttaagatctt atttttagat cttttttttt aaaacctttt
     1321 taggtcttgt aacaccatga ttttaaagat gttctggcaa gataagtgga gcttttgggg
     1381 gtgaaaggtg gagcttttgg gggtgaaagg tggagctttt gggggtgaaa ggtggagctt
     1441 ttgggggtgt aatccacaga aaaagggggt tgcaaaaaaa aaacttccac ccatatgata
     1501 agtggcgtac agtaaaaaaa cgccaccaat agcaacgagc aagcgaaatg tcagatctga
     1561 tagcttacaa atcaaacgcc cttatcgagg caagctacaa gctcactttg caggaacagc
     1621 gcttcctgct ggtctgtatc agccgcctaa aatccggaga agattcccct tcgccggatg
     1681 aacaaaaaac catgaccatc acggctgcag agtttcatga tgcctttcct gacatgggac
     1741 ggcagcacgc tgaagctagg ctcaaggaag cgatagatcg cctctgggac aggtcagtaa
     1801 tcatcaagaa cgaggaaaaa cgagaagagt tccgctgggt gcagtaccgg gcgcagtatt
     1861 tcaaaggtga ggcgaaagta gatatcacct tctccgatgc catcatgccg tatctgacac
     1921 agctcaaggg gcagtttacg cgggtagtcg tgaagaacgt ctccagcctt tccagcacct
     1981 acagcatccg catttacgag ctgctccagc agttccgcag caccggtgac agaacgatag
     2041 ctatcgatga tttccgaacc atgcttgccg ttgaagataa atatcaacaa ttcaaagact
     2101 tgaataagat gataatcaag ccagccatcg ccgaactgaa cgaaaaaagc gatctggttg
     2161 taactgttga gacgatcaaa aaggggcgca cggttgtagc cctgcatttt cgcttcaagg
     2221 aagacaaaca gatcaagatg acgatctaaa atcgatttta aggcacctaa aacgctccct
     2281 gctgcgtttt ttttgtgtta gtagtgcgaa ggtactattg aagtgaaaaa ttgcgtcaaa
     2341 aacgctctga tgcagcgaaa aaagaccccc caatttttgg gggcggaaaa acaaaactat
     2401 gcttgcgtta tgtgagtgtg cttcttgcat tcatccctgt cgatgaacat cgacgaacat
     2461 aacacgatgt taaacaaaac aaaatattgt tcacattcac tgtgaccaac cgcgatcatg
     2521 tctcctctcg gtggtgtgtg ttcgtgcatc ttcctcgcgc tgccattcct cccaggtacg
     2581 attaaaaccc agcgagcgga acagatgatc cggctgtttt tctggtgtgt tgacggcagc
     2641 aaaaagcggt gcctcacggg ttttcagatg aaactgcatc agttctggtg caggtgatat
     2701 gtggctgtct gcatcgtgat ttacggcaac accataccct gcataccgta accccttatc
     2761 ggtcagcgca ctgatgacca gatgcctgtt atcggctgag ggaacggcaa acgcgggata
     2821 cagcgtacca ttaagccgga taacaccgcc cagagagtca tccacgccca tgaccagcaa
     2881 aagctgaagc tggcgggttt cccggtcgac agaactcatg accggcatca gcgtcggtgg
     2941 cagacgtccc tgcataaaat ccaccttcct tgccgctggt gatggcatgg ctggctggcg
     3001 gtcttctcca agacagaaag actccggcca tgtcttcggc tggcggagat tgcgggtatg
     3061 ttgcggccat gtctggcaaa cctgcgtcag cagttgctca aaggttgcag aggaataaac
     3121 acgttgcagt ggcggacgtt ggccgatttc gcttcggtca cagatataac cactcctgtc
     3181 acgcattaaa tcaacactct caaagattct ggtgcggtat ttctccagct cgttccccca
     3241 gagcgtatat tctccgttcc ggttgtgaag cagaaggtaa caggtcattt ccccgttacc
     3301 aggcatggga gcctcgccat accccgtaac gactgctctc agcacccctg aatccataac
     3361 ggcattgtca tttttcctga gctgctccgc gcgactacgt tcagtatctt cctgttcctt
     3421 ccggcgcaat tcctcttcac gtttaagcaa ctgggctctg agagctgcaa ggccggattc
     3481 cccccactgc tgccactgtt tttcaaatgc catctgactc gcgggaatgt cctgtttctc
     3541 caccttccgg taatgctgtc gtaatttttc catttgctgg ctcagagact ggatttttcc
     3601 ctgctcacta cggtattccg gtgccgccca ttgcatcagg ggaatacggg aaaaaccgga
     3661 cgaaaacggc gatgattttt tcaggctggc agcatggctc atcgcttcct ttcgttcggt
     3721 gttcagctct tcccagcgtc tgcgcgtcgc ctgcatatct gccatcatcg acatggcatt
     3781 actggtgagt ttccatgccg agttactgtc cttcggcaac gcttcgataa tgtcacgtag
     3841 tgtgtacgaa gttgcgttgt caggaagccc gatattcaga aggtcagcca cacggcgggc
     3901 aggctctgtc agccctccgt tcctgagttc ggtgcgggaa atctgtattt ccaggttaag
     3961 cctgttaatc agacgattct gttcgccgcg ttctgtttcg ataccgcgtg tttccatcgc
     4021 acaggcagac tttccgaggt gaataccagg cgttttctca agc
//
