LOCUS       NZ_CP023842             8809 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 4/1-2 plasmid p4_1_2.3, complete
            sequence.
ACCESSION   NZ_CP023842
VERSION     NZ_CP023842.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07760932
            Assembly: GCF_003856595.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   Sandegren,L.
  TITLE     Dynamics of resistance plasmids in extended spectrum
            beta-lactamase-producing Enterobacteriaceae in the gut microflora of
            patients during 1 year post UTI
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8809)
  AUTHORS   Sandegren,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2017) Medical Biochemistry and Microbiology,
            Uppsala University, Husargatan 3, Uppsala SE-75123, Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics workbench v. 10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 13x
            Sequencing Technology             :: Pacific bio + Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:31:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,533
            CDSs (total)                      :: 5,409
            Genes (coding)                    :: 5,296
            CDSs (with protein)               :: 5,296
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 49 of 113
            Pseudo Genes (incomplete)         :: 65 of 113
            Pseudo Genes (internal stop)      :: 19 of 113
            Pseudo Genes (multiple problems)  :: 16 of 113
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023842.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8809
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="4/1-2"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4_1_2.3"
                     /geo_loc_name="Sweden"
                     /collection_date="2009"
     gene            complement(join(8761..8809,1..194))
                     /locus_tag="CRT36_RS29280"
     CDS             complement(join(8761..8809,1..194))
                     /locus_tag="CRT36_RS29280"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(560..1192)
                     /locus_tag="CRT36_RS28120"
                     /old_locus_tag="CRT36_28220"
     CDS             complement(560..1192)
                     /locus_tag="CRT36_RS28120"
                     /old_locus_tag="CRT36_28220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(1192..2307)
                     /locus_tag="CRT36_RS28125"
                     /old_locus_tag="CRT36_28225"
     CDS             complement(1192..2307)
                     /locus_tag="CRT36_RS28125"
                     /old_locus_tag="CRT36_28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(2652..2765)
                     /locus_tag="CRT36_RS28130"
                     /old_locus_tag="CRT36_28230"
                     /pseudo=""
     CDS             complement(2652..2765)
                     /locus_tag="CRT36_RS28130"
                     /old_locus_tag="CRT36_28230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3181..3372
                     /locus_tag="CRT36_RS28140"
                     /old_locus_tag="CRT36_28240"
     CDS             3181..3372
                     /locus_tag="CRT36_RS28140"
                     /old_locus_tag="CRT36_28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3372..3773
                     /locus_tag="CRT36_RS28145"
                     /old_locus_tag="CRT36_28245"
     CDS             3372..3773
                     /locus_tag="CRT36_RS28145"
                     /old_locus_tag="CRT36_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4616..4825)
                     /locus_tag="CRT36_RS28155"
                     /old_locus_tag="CRT36_28250"
     CDS             complement(4616..4825)
                     /locus_tag="CRT36_RS28155"
                     /old_locus_tag="CRT36_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            5213..5395
                     /locus_tag="CRT36_RS28160"
                     /old_locus_tag="CRT36_28255"
     CDS             5213..5395
                     /locus_tag="CRT36_RS28160"
                     /old_locus_tag="CRT36_28255"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080882243.1"
                     /translation="MGKELSLNKIYDKNSRYINDSRGHELGKEKKVFDFEVEMIKKRRE
                     DAIRKIIEDSKSLKW"
                     /name="hypothetical protein"
     gene            5408..7090
                     /locus_tag="CRT36_RS28165"
                     /old_locus_tag="CRT36_28260"
     CDS             5408..7090
                     /locus_tag="CRT36_RS28165"
                     /old_locus_tag="CRT36_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697690.1"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage dCTP deaminase"
                     /protein_id="WP_227538319.1"
                     /translation="MSLARKLREKRSNPIHSANEDSSGGDSINQITSRQSMDLVIGFCG
                     AVGSGIKSIVDVTEEELDDLGYDVYRIRVSELMQKLFSEWRDDIVTPFDRYHKLQDFGD
                     ALRNKYYPHILSEAVIHEIKIEKGIDTSSKKNKKAFLVDQLKHQDEIELLRMVYQHNFY
                     LLGVIRSEVERKRNLRDEGLSKSEVDIVIHHDRKSESDYGQQTAKAILDSDYFVKNNQG
                     QRAHLKAKVKRFIGLIHGLNGLTPTTYEKGMYAAHSASLQSACLSRQVGAAILDVNGNL
                     ISVGRNDVPKFNGGLYSADDGVNDHRCVYKGGKCYNEVRKLKIKSKISQILMSESACEL
                     GVKLTEDQAEQLADVIYKKTPVSSIIEYSRSIHAEMDAITSLARLGNGGFSDKVLYTTT
                     FPCHNCARHIVAVGITKVVYIEPYEKSLALELHDDAITEVNEHGKVIFESFEGVSPRRY
                     QKFFFSTDERKDSFGNAEKYSTKYKNHIDIQFIDSYLDFENRVVDIFRTNTNIDSGNHV
                     GRTDSTINLSSQPVPPVPPVPPVPSVPPVPPVPPVPPVPPDSK"
                     /name="anti-phage dCTP deaminase"
     gene            complement(7294..7407)
                     /locus_tag="CRT36_RS29775"
                     /old_locus_tag="CRT36_28270"
     CDS             complement(7294..7407)
                     /locus_tag="CRT36_RS29775"
                     /old_locus_tag="CRT36_28270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_080882241.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            7823..8014
                     /locus_tag="CRT36_RS28185"
                     /old_locus_tag="CRT36_28280"
     CDS             7823..8014
                     /locus_tag="CRT36_RS28185"
                     /old_locus_tag="CRT36_28280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8014..8415
                     /locus_tag="CRT36_RS28190"
                     /old_locus_tag="CRT36_28285"
     CDS             8014..8415
                     /locus_tag="CRT36_RS28190"
                     /old_locus_tag="CRT36_28285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2653..3060)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
     Typing          complement(7295..7702)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.355"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gacttgccat aacgtgattg ccggccgtcg acgaacccga ttcctgaagg tgctgaaaac
       61 cggttttccg gctgcgccgg caacgcttca cggccacgca ataaattgcg tccccgttca
      121 ccgttttcgg aggagaagaa cgccggaaac aggacttccc ctggctgaat ggtgtgaatt
      181 tccgcgctat gcacttgcgc gcatactcat gcatgccgta aaaacagagc ctgcgcgttt
      241 ctggcgggtt ttcgggtggt ttgttgcctg ttttaccggt ttcccgtcag aaacgtcctg
      301 agtccgtttt agcggtgcgt acaattatga ttttatgtta aattgtttta agtgaataga
      361 gagaatataa cttaagggag catcttgtgc tgggtctttt acattagatc tgcacaatca
      421 ttgttttggc atctggtgat gtctttagga cgtttgagat tgctgataac taaagtgtat
      481 gatgctttct atacgttatc cggcgtttaa taacaccctg gtgataagcc agcccgtgaa
      541 gggctggctt gatttggatt tatatttttg aaataaaatc ttttatttca tcaaagcaat
      601 tgtctgctgt gtttattatt ccatttttca tggatattcc tattcttgag tcggattctc
      661 tattgcagct aagatagact tgtaccttag cttttttttc gtttgttgga taagtgtact
      721 ctttgtcact cgctgagatg tcatctttat tatttttaat tttttcgaaa aattctatta
      781 tgtattcata taacgctcga gtgcttgcgt gttcaattat cagatcttca atggcacctt
      841 gcttgtcttt tcctggcatt ataaagaaag aaatttttgt attgccattc atagctatct
      901 cgttatgcga gctgggggag gagatttgta gttctcgcaa tgtggcaatt attgatcttt
      961 ctgtgtcaga aaatgatgtg tcagcatcta atataataaa aatactttct ttttcatcga
     1021 aagaatcaat gtttttgaat gctctaagtg cttgttttaa ttttgatttt ccatttattt
     1081 tttcgacaaa aacatcggta atgccttgta tttcaagtag gttgcataga aaatgaaaat
     1141 catcatcacc ctcaacgaaa atgatctttt tgctattatt tatgtctttc attatcttac
     1201 ctcccagtcg ttctcaactg agtaggagaa atcatccata ttgaattgta cggctgttgg
     1261 ttttagggag ttcttttctc ttcctagtct aatataagcg atatcttcat ttttgctatc
     1321 tatttcgttt attgctcgta tcacatcttg actatgagtt gttgcaaata tttgattgtt
     1381 gttttgtttt gcagatgaaa ttaatgattt tattatgtct ttaattactg agtaatgtat
     1441 tccgttctcg atttcatcaa taagaataat tgagttttcc tgtaccagca ctgagctgat
     1501 aaaagataat gctcgactga taccctcgcc aagcattgat atttcattta gctcactaaa
     1561 tccaatgtct aaatatattt ccttattgtt acctatcgca gatattgcta tatctacaat
     1621 tctgtcatct atttttttta agttttctag tatgtctttt ttcctttttt gtgttagtag
     1681 gctagataca tttgtaattg ttgcctcttt gtttatcttt ctggatgttg ttattattgt
     1741 tgctgtctta aatttataat taatcccatc cctttcgttt tttatttcgg attttatttc
     1801 tacgccatct tctttcaatt gcatagagaa aagcttattg gaaaccctgt ttttcgcatt
     1861 gggtgtctcg gtcacattta tttttaaaga gtttactgtg gcctgagaac cgatagtttg
     1921 acgaggtatg ttttggttag atagtacggt gtcaacgttt agagacaaac ttgattctaa
     1981 tctctgattt gtttctgttt catatgtttg tgttacttca gagcgttcat ctcgaatgac
     2041 tatggacatc ttttgtgatg tgtccatatc atgaaagtaa ctaacccaaa atggcttgtt
     2101 tttgtttatt tctgccattt cttttctgaa tgcttgaatg tttaacaaaa cagccgggtt
     2161 ttttacatca taaaaacaaa aaatagcatc cagtatagat gttttcccgt agttgttttt
     2221 cccagctatg atatttaaag tctttaattg ctttagatat agctcattga agcatttgaa
     2281 gttctttaaa cttatttcct ttatcatgat agcctctgtt cgtgtttttg ttatatgcca
     2341 tacgatttgt tagtacctta ataaaatgta cagctttcat tgtgattagc tagactttat
     2401 gtcaaagatt aactttgatt gcttgtgttt taatcgattt tttttacttt cttcttgaga
     2461 ttatctggca gcagccgtaa gcttccggct gtgaacgaaa aaaaccgcct tggcgggcgg
     2521 ttttttcgaa aggttcagta gagttggcgc tctatgaacc gaggtaactg gcttaaaggg
     2581 agcgcagtca ccaaaactgt tcgtttagtt tagctttaac tggcgtataa cttcaagact
     2641 aactccctaa atcagttacc agtggctgct gccagtggcg ctttgtcgtg tctttccggg
     2701 ttggactcaa gacgatagtt accggattag gcgcagcagt cggactgaac ggggggttcg
     2761 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg
     2821 agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
     2881 gcgcacgagg gagccatcag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2941 ccaccactga tttgagcgtc agattccgtg atgctcgtca ggggggcgga gcctatggaa
     3001 aaacggccgc tgtgcggcct ttccttcttt cctccatgcg attactcggt tttctcgagc
     3061 gtttctacgc atgctcctgc cgctcgccgc agccgaacgg caggagcgta gcgactgagt
     3121 gagcgaggaa gcggaaaaga ggagatccct acactgacca ctgacgctct gaggcctctt
     3181 atgacgaaac aacagaaaac agcgctgaac atggctaaat tcatccagaa tcagtctctg
     3241 ctcctgctgg agaagcttaa cgagcttgat ctcgatgtcg aggccgacct ttgtgagaaa
     3301 ctccacgatg atgcggagca tcttttccgt acactgtcct cgagactgga tgcattacag
     3361 gatggtaact gatggccaga gtgtctatta gtgaggctgc ccgactgacg ggtaagagta
     3421 gaacaacact gcacaggtta attaaagcag gtgacctgtc cacctgtagc ggtgagcgca
     3481 atgcaaaaat ggtagatact tcagagcttt tacgtgtctt tggccccttt gagcaaccga
     3541 agggtgaaca ccattctgga caggtctctg gacagcgtga tacagcctct gctgaccaga
     3601 gtgaacaggt gatccgtcat ctgaaacagg agattgaaca cctgaacacc ttacttttgt
     3661 ccaaggattc gcacattgac agcctgaaac aggccatgct ccttcttgaa aatcaccagg
     3721 caaaattaac tgaatccccg gcgccatggt ggaaattctg gaaaaaatcc tgaccccgtc
     3781 atgcttcagt tttcctctcc ggccatcccg gggagttcct gaaccttccc aaaaatcgtc
     3841 agatctctga acgctcctca ccactaaaaa tacccacacc ccacgcaaaa ttaatatttt
     3901 gcagatttgt atttaatatc agtgggttac tttcctgaaa tttcgttttt taaattttat
     3961 ttatatagat ccgactctat gacgactcgt catagagtcc ccggcgagtc gtcatagagt
     4021 cggtaacgac tcaggactat aaaaacaata agttaataac gacccgactc tagacacgac
     4081 tccccgtttg gttgtttttt agacgcggcg actctgggta cgactctagt gaagagtcgc
     4141 cggtgaatcg tgtggtgagt cgtccggcga cttgccataa cgtgattgcc ggccgtcgac
     4201 gaacccgatt cctgaacgtg ctgaaaaccg gttttccggc tgcgccggca acgcttcacg
     4261 gccacgcaat aaattgcgtc cccgttcacc gttttcggat ggaaagaacg ccggaaacag
     4321 gacttactct ggctgaatgg cgtgaatttt cgcgctatgc acttgcgtgc atactcatgc
     4381 atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt cgggtggttt gttgcctgtt
     4441 ttaccggttt tccgtcagaa acgccctgag gccgttttag cggtgcgtac aatcatgcct
     4501 ttatgttaaa taacagatgc aaaaaagccc cagaaggggc ctgaaggttt tcctgctaag
     4561 ggacgaaacg ggccgcctcc cggcggcctg ttcggcagct ccctttgcga ccatcttatt
     4621 tggtcagttc agccgtcatg tggacacggt tattgaactg agcgctggtg attttgtatg
     4681 acgcaccttg ttcagcggcc tgtgcggcaa tttttgcttc agcacgatcc agggtggagg
     4741 cggtagcgct gacgctctga gcaaaagcac cgaaagacat catggataga gcggcaactg
     4801 caacgaaagt tttgatggat ttcatggtcg tattccttaa gtgattttgt tctgtatggg
     4861 gcgttgttgc cctgatgtga taaatgatag gacgaccatt agttgattaa aatcgaaaca
     4921 atttgcggat atcattcaaa aaaaaagaac aagaattgag cgcatcaaga ccagaggttt
     4981 atctctggat tttaggtacc aaatgaggtg gtggatagaa atgttaacat tgatgatttt
     5041 attttaacaa attgaaaaat aatggttagt ttggcgtaat ctctcttgct tttctcttgc
     5101 cgaatttcct aatgtttagg ataagaaaaa aaaatctcta tcgattacgt ttcttggcta
     5161 ttgcctgtta taaagataac agtaaaatcc atgcgtcttg caggagggtg tgatgggtaa
     5221 agagctatct ctaaataaaa tttatgataa gaattctaga tatattaatg attctagagg
     5281 gcatgagctg ggaaaggaaa agaaagtttt cgattttgaa gttgaaatga ttaaaaaaag
     5341 aagagaggac gctataagga agataattga ggattcaaaa tcattaaaat ggtaagtgag
     5401 gtcttttatg agcctggcta gaaagttaag agaaaaaaga agtaatccga ttcatagtgc
     5461 taacgaggac tcctctggag gggatagcat aaaccaaata acctccagac agtctatgga
     5521 tcttgttata ggattttgcg gtgctgttgg ctcaggaata aagtcaattg ttgatgtgac
     5581 tgaagaagaa ctagatgatc taggatatga tgtttatcgc ataagagtta gcgagcttat
     5641 gcaaaaactt ttttcagaat ggcgtgatga tattgttact ccgtttgata gatatcataa
     5701 gctgcaagat tttggtgatg cgttaagaaa taaatattac ccacatatac tatctgaagc
     5761 tgtaatccat gaaataaaaa tagaaaaagg cattgacacc tcaagtaaaa aaaacaaaaa
     5821 ggcttttttg gtcgatcagt tgaagcatca ggatgaaatt gaacttttaa ggatggtcta
     5881 ccaacataat ttttatttac taggtgtaat tagaagtgaa gtagaaagaa agcgtaatct
     5941 tagagatgaa gggctgagta agtcagaagt cgatatagtt attcatcacg atagaaaatc
     6001 agagagcgat tatggtcagc agactgcgaa agctatactt gactctgatt attttgttaa
     6061 gaataatcaa ggacaaaggg cgcaccttaa ggctaaggtt aagcgtttta ttggcttgat
     6121 acatgggcta aatggtttaa ctccaaccac gtatgaaaaa ggtatgtatg cagctcattc
     6181 agcatctctg caatctgcat gcctttctcg tcaagttggt gctgctatat tggatgttaa
     6241 tgggaatctg atttccgttg ggcgtaatga tgtacctaaa ttcaatggtg ggctttatag
     6301 tgcggatgat ggtgtcaatg atcatcgttg tgtatacaaa ggtggtaaat gttataatga
     6361 agttagaaaa ctaaagatta aaagcaaaat ttcacaaata cttatgtcgg aaagcgcttg
     6421 tgaacttggt gttaaattaa cagaagatca agcagagcaa ctggctgatg taatatataa
     6481 aaaaacacca gtatcttcta ttatagagta ctctcgctca attcatgcag agatggatgc
     6541 tattacatca ttagctagac taggtaatgg aggtttttct gataaagttt tatatacaac
     6601 cacatttcct tgtcataact gcgcgagaca tattgtcgct gttggaataa caaaagttgt
     6661 ttatatagag ccatatgaaa aaagcctcgc tttagaattg catgatgatg ctataaccga
     6721 ggtgaatgag catgggaagg ttatatttga atcctttgaa ggtgtatcac ctcgtaggta
     6781 tcaaaaattc ttcttttcta cagatgaaag gaaagatagt ttcggtaacg cagaaaagta
     6841 ctccactaaa tataaaaatc acattgatat acagtttatt gatagctatt tagatttcga
     6901 aaatcgagtc gttgatattt ttagaactaa cacaaatatt gattctggca atcatgtcgg
     6961 gagaactgat tcgactatta atttatcttc ccagcccgta ccgcccgtac cgcccgtacc
     7021 gcccgtaccg tccgtaccgc cagtaccgcc agtaccacca gtaccaccag taccaccaga
     7081 ctcgaaatga agatctgttg actctgatgt tatacagagc ttaaccttga gacgagaaag
     7141 aaaaaaaccg cctttgcggg gcggtttttt taaggttcta gagtttgcgg ctctatgaac
     7201 caaggtaact ggcttagagg agcgcagtca ccaaaacttg ttctttcagt ttagccttaa
     7261 ctggcgcata acttcaagac taactcctct aaatcagtta ccggtggctg ctgccagtgg
     7321 tgcttttgca tgtcttaccg ggttggactc aagatgatag ttaccggata agacgcagca
     7381 gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga
     7441 actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa
     7501 accgaatggc aggaacagga gagcgcacga gggagccatc agggggaaac gcctggtatc
     7561 tttatagtcc tgtcgggttt cgccgccact gatttgagcg tcagattccg tgatgctcgt
     7621 caggggggcg gagcctatgg aaaaacggcc gctgtgcggc ctttcctttt ccccatcacg
     7681 agaccgctcg gttttctcga gcttttacac gcatgctcct gccgctcgcc gcagccgaac
     7741 ggcaggagcg tagcgactga gtgagcgagg aagcggaaaa gagactgttc ttacactgag
     7801 cactgacgct ctgaggcttc ttatgacgaa acaacagaaa acagcgctga acatggctaa
     7861 attcatccag aatcagtctc tgctcctgct ggagaagctt aacgagcttg atctcgatgt
     7921 tgaggccgac ctttgtgaga aactccacga tgatgcggag catcttttcc gtacactgtc
     7981 ctcgagactg gatgaattac aggatggtaa ctgatggcca gagtgtctat tagtgaggct
     8041 gcccgactga cgggtaagag tagaacaaca ctgcacaggt tgattaaagc aggtgacctg
     8101 tccacctgta gcggtgaacg caatgcaaaa atggtagata cttcagagct tttacgtgtc
     8161 tttggtccct ttgagcaacc gaagggtgaa caccattctg gacaggtctc tgaacagcgt
     8221 gatacagcct ctgctgacca gagtgaacag gtgattcgtc atctgaaaca ggagattgaa
     8281 cacctgaata ccttactttt gtccaaggat tcgcacattg acagcctgaa acaggccatg
     8341 ctccttctcg aaaatcacca ggcaaaatta actgaatccc cggcgccatg gtggaaattc
     8401 tggaaaaaat cctgaccccg tcatgcttca gttttcctct ccggccatcc cggggagttc
     8461 ctgaaccttc ccaaaaatcg tcagatctct gaacgctcct caccactaaa aatacccaca
     8521 ccccacgcaa aattaaaatt ttgcagattc cttatttaca acaatgggtt aatgaaaaat
     8581 aaatgtgcgg tgatgtgtta caaagtgcag atccaagtcg tggacgactc tatggcgaat
     8641 cgctgacgaa ttgcccacga ttcatatgtg aatcacgctt tattatcata ttgttatgat
     8701 gaatcgactc ttgattcaat tcatcacttt gggtgttttt tgctcatggt gactcttgat
     8761 tcaattcacg attcgaatcg ccggtgaatc gtgtggtgag tcgtccggc
//
