LOCUS       NZ_CP023830             7939 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain 4/4 plasmid p4_4.4, complete sequence.
ACCESSION   NZ_CP023830
VERSION     NZ_CP023830.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07760938
            Assembly: GCF_003856655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Sandegren,L.
  TITLE     Dynamics of resistance plasmids in extended spectrum
            beta-lactamase-producing Enterobacteriaceae in the gut microflora of
            patients during 1 year post UTI
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Sandegren,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2017) Medical Biochemistry and Microbiology,
            Uppsala University, Husargatan 3, Uppsala SE-75123, Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics workbench v. 10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 24x
            Sequencing Technology             :: Pacific bio + Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003856655.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 02:24:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,270
            Genes (coding)                    :: 5,051
            CDSs (with protein)               :: 5,051
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 81 of 219
            Pseudo Genes (incomplete)         :: 165 of 219
            Pseudo Genes (internal stop)      :: 39 of 219
            Pseudo Genes (multiple problems)  :: 56 of 219
            Pseudo Genes (short protein)      :: 1 of 219
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023830.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="4/4"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p4_4.4"
                     /geo_loc_name="Sweden"
                     /collection_date="2009"
     gene            complement(173..331)
                     /locus_tag="CRT43_RS28495"
                     /old_locus_tag="CRT43_28495"
     CDS             complement(173..331)
                     /locus_tag="CRT43_RS28495"
                     /old_locus_tag="CRT43_28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(990..1181)
                     /locus_tag="CRT43_RS27370"
                     /old_locus_tag="CRT43_28510"
     CDS             complement(990..1181)
                     /locus_tag="CRT43_RS27370"
                     /old_locus_tag="CRT43_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1246..1569
                     /locus_tag="CRT43_RS27375"
                     /old_locus_tag="CRT43_28515"
     CDS             1246..1569
                     /locus_tag="CRT43_RS27375"
                     /old_locus_tag="CRT43_28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955993.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1778..>2209
                     /locus_tag="CRT43_RS28275"
                     /pseudo=""
     CDS             1778..>2209
                     /locus_tag="CRT43_RS28275"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2247..2765
                     /locus_tag="CRT43_RS27650"
     CDS             2247..2765
                     /locus_tag="CRT43_RS27650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            2772..3005
                     /locus_tag="CRT43_RS27655"
     CDS             2772..3005
                     /locus_tag="CRT43_RS27655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3716..3955
                     /locus_tag="CRT43_RS27395"
                     /old_locus_tag="CRT43_28535"
     CDS             3716..3955
                     /locus_tag="CRT43_RS27395"
                     /old_locus_tag="CRT43_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            3952..4314
                     /locus_tag="CRT43_RS27400"
                     /old_locus_tag="CRT43_28540"
     CDS             3952..4314
                     /locus_tag="CRT43_RS27400"
                     /old_locus_tag="CRT43_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_124800797.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYEVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(4339..5232)
                     /locus_tag="CRT43_RS27405"
                     /old_locus_tag="CRT43_28545"
     CDS             complement(4339..5232)
                     /locus_tag="CRT43_RS27405"
                     /old_locus_tag="CRT43_28545"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(5235..6827)
                     /locus_tag="CRT43_RS27410"
                     /old_locus_tag="CRT43_28550"
     CDS             complement(5235..6827)
                     /locus_tag="CRT43_RS27410"
                     /old_locus_tag="CRT43_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(6866..7564)
                     /locus_tag="CRT43_RS27415"
                     /old_locus_tag="CRT43_28555"
     CDS             complement(6866..7564)
                     /locus_tag="CRT43_RS27415"
                     /old_locus_tag="CRT43_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000939065.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     Typing          835..914
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.78"
                     /coverage="92.0"
                     /evalue="8.94e-33"
     Typing          complement(260..802)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.679"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          1214..1563
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.42e-67"
     Typing          1560..3109
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgccagta aacgaaaaaa
       61 ccgcctgggg aggcggttta gtcgaaggtt aagtcagttg gggactgctt aaccgggtaa
      121 ctggcttggt ggagcacaga taccaaatac tgtccttcta gtgtagccgc agttaggcca
      181 ccacttcaag aactcttaat atctcaatcc accttgtcca gttaccagtg gctgctgcca
      241 gtggcgcttt gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      301 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      361 ccgagccgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      421 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      481 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      541 gtcgattttt atgatgctcg tcaggggggc ggagcctatg gaaaaacgcc aacggcgcag
      601 ccttttcctg gttctcgttt tttgctcaca tgtttctttt ggcgttatcc cctgattctg
      661 tggataaccg catctccgct tttgagtgag cagacaccgc tcgccgcagc cgaacgaccg
      721 agtgtagcga gtcagtgagc gaggaagcgg aagagcgccg gaacgtgcat tttctcctta
      781 cgcatctgtg cggcatttca catcggacat ggtgcgcttt ccatacaatt cgtactgatg
      841 ccgcatagtt aagccagtat acactccgct atcgctacgt gactggttca gggcttcgcc
      901 ccgaaacccc ctgacgcgcc ctgaggggct tgtctgctcc cggcatccgc tcacagacaa
      961 gctgttaccg tctccgggag ctgtatgtgt cagaggtttt caccgtcatc cccgaagcgt
     1021 gcgaggcagc tgcggtaaag ctcgtcggcg tggtcgtgaa gtgattcaca aatatcggcc
     1081 tgttcatctg cgtccagttc gttgagtttc tccagcagcg ttaatgtctg gcttctgata
     1141 aaacgggcca tgttaagggc ggttttttcc tgtttagtca ctgatgcctc cgtgtaaggg
     1201 ggatttctgt tttatggggt aatgataccg atgaaacgcg agaggatgct caccatacgg
     1261 gttactgatg atgaacatgc ccggttactg gaacgctgtg agggtaaaca gctggcggta
     1321 tggatgcggc gggtctgcct gggtgagccg gttgcacgct ccgggaagct gccgacactg
     1381 gcaccgccgt tactgcgtca gctggccgcc atcgggaata acctgaatca gacagcgagg
     1441 aaggtgaaca gcgggcagtg gtcttccggt gatcgggttc aggtgttggc tgcgctgatg
     1501 gccatcgggg aggagctgcg tcggctgcgt ctggccgtca gggaacaggg cgcgcgggat
     1561 gattgttaaa tttcatgcca ggggaaaagg cggtggcagt ggtccggttg attacctgct
     1621 gggaagggag cgaaaccggg aaggggcaac ggtgcttcag ggtaatccgg aagaagtccg
     1681 ggaactcatc gatgccacgc catttgcgaa gaaatacacg tcaggtgttc tgtcgttcgc
     1741 ggagaaggag ctgccgccgg gaggacgtga aaaggtgatg gcgagctttg agcgtgtact
     1801 gatgcccggt ctcgaaaaga atcagtacag catcctgtgg gtggagcacc aggacaaggg
     1861 acggcttgag ctgaattttg tcattccgaa catggagcta cagaccggaa aacgcctcca
     1921 gccgtactac gaccgggcag acaggccgag aattgatgcc tggcagacgc tggtgaatca
     1981 ccattacggg ctgcatgacc ccaacgcccc ggagaaccgc cggatactga ctctccctga
     2041 taacctgccg gaaacgaaac aggcgcttgc agagagcgtc acacgcggga tagacgctct
     2101 ttatcatgtc ggggagataa aaggtcgtca ggatgtgatt caggcgctca cagaggcagg
     2161 gcttgaagtg gtcagggtga tgcgaagcag tatcagcatt gcagacccga acggtgggaa
     2221 gaatatcagg ctgaaaggag cattttatga gcaatctttt acagacgggc gcggagttcg
     2281 agaaaaagct gaaagagaga gccgaatcta cagagacaat gctgaacgac gagttcagca
     2341 agctcgaaaa atctgtaagc aaggctgtga catcaaacga gacgaaaatc aaagacgcta
     2401 tagccctgtt cacagcttcg acagaggaat cactgaaaaa acaccgggaa ggggtgaaag
     2461 aggcgatgat gcagcacagg aaggacgtgt taaagctggc agggaatacg ggcatgatgt
     2521 tactggggat ggtccttttc ctgtttaccg tgagtggcgg gacgctctgg tatcttggcg
     2581 ggatgataca ggcgaacctg gaagaaatca ggatacagga agaaacattg cagaaactga
     2641 acgcgaagac atggggcgtg gagtttgtgc aggacgggag aaggaaattt ctggttatcc
     2701 ctcaggggaa atcggcaacg gtgattccct atcaggggaa ggactgggta caactgacgg
     2761 agtgacacag agtgacggag ctggaaagac atttgctgaa cgccttagag caactgcaac
     2821 aggactatat gcagcggctg aacgaatggg agagcgcctt cgtggaattg cagaaaatgt
     2881 tttcgcttac gcaacgggac aacgcgatgc tgaacgagcg ggtcatgcag ttgagtcagc
     2941 aggtgcagca cttgagcgag cagacagaac gcttgagcca gttatacagc gagaacagga
     3001 gataagggat gaacggctga tacaggaacg cgaacatgag ttatccctgg agcgtgagcg
     3061 ccagctggaa atacaggaac ggacacagga tggcccttcg ctgggatggt aatcagagac
     3121 aggctaaaat gttgtcagcg cgtttccggt gcattttttg ggataatggt tggttcgggg
     3181 gcaccgggaa cgcgcttcag tcagaccttg ctgaaaacac aggaacgcgt taaaacgcat
     3241 acgggcgcga ttctggtgtg tcttttgttg ctgtgttttg cctctattgc cagatcgcgc
     3301 tcattcccct gaacttctgt tttcccgctc cggtccccgt cttcttcgct tccgaaagca
     3361 tgaaaaaaat ccgtttcagc tgtctctggc tggcaattcc tgcgtgattc acatggctgc
     3421 atagctatgc atgtggttaa aatttaccgg gtgcgatcgc ggcagttttt cgggtggttt
     3481 gttgccattt ttacctgtct gctgccgtga tcgcgccgaa cgcgtttcag cggtgcgtac
     3541 aataagggat tatggtaaat tatcttgttc cttagagaat gatttgaggt ggtatcctgc
     3601 acttatttaa atatgcttgc ttgggaaatt ccaggttgca gtatggttga gttgtaattt
     3661 tcctatgata ggaaaaatgt tatattatta atttatttgt gaggcttcat aaagaatgaa
     3721 aatttctgat attgaaaaaa acatatataa aggactagct gaatttattt cacaaaaaat
     3781 cagagatgaa catgatatat gccttggaca gtttgaatca gaggaattac ttgatgagat
     3841 tcttaaaaga gtaatcccag tcatatataa tgtggctatt gatgatgcta taaaatcagt
     3901 aaggaatgtt ggggatagat tagaggagga attagatatg aggaaggtag tgtgactaat
     3961 tttaaatcag acttaaatct aataagaaaa gaatggtata atgcatttta ttcaggtaat
     4021 actgaaatgc tagattactt ggaagctgat tggtttattt caacaaatgg tcaaaaagtt
     4081 atctataaaa aacatcagtt acgaaagatt gatatgcgtc cagcgaggat tttacctagc
     4141 atagaaagat atgaatatga ggtcgtaatg cgtgagttta aaggcattgc ttgtgtttca
     4201 gggaaagctg aaataaagca aaatgatgga aatatattaa tggcttttat tgagaattgg
     4261 ataaaggtta atggtggatg gaaaatgcag tttatatcgt atgaaaaaat ctaaggggaa
     4321 gcccccttag attaatggtt atatatgttc aagttttatt tttttatagc gataagttct
     4381 gctttgagct ttaagcatgt tttctgcaaa ctgaatatca aaactattta tagcattagt
     4441 tattatagtt tttatttttt ctttatcaga taatgaatca ccatgctcta taggttcccg
     4501 catatatgga ttgtttttta attcatcaaa taatgtttta aatagttcta atcgacgagg
     4561 tcttataact gtaaaagata tatgaagaga gtgtttgctc tgagtcgtaa cattgtgcca
     4621 gtatccagca ggaagatata tggcctgacc tggggttata tcaaactcat aatctgggga
     4681 ggtattaggt ggtagataat cgaagcttct ttcatcttcg agagggtatg aataggtagg
     4741 gttgtaaact ttccattttt ttattccctc aatttgaaca gctattacat catgattgtc
     4801 aaaatgaagc ccaaagcttg gtgttgctgt aaatgcagca tatagattag cactacatgt
     4861 acattctaac tctttagata accatagtct gctttcatct acagattcaa agaatgactg
     4921 gcatcgatct actataaggg ttgcgccatc acgtaagcat ttatatagct gatggcgatt
     4981 tatatgtggt gttctatcgc ctgtttggct atatgtgtat cttataaatc ctttataccc
     5041 cctaatttcg ggaaagttgt catttgccat tctaactcta ggatagtgca atatatcttt
     5101 ttcaagcaat ttattaagat cattccagtt gattaagttt ttataattaa catccgtgaa
     5161 tacaaacgga tatttctgga aattatattt cttaaatttt tctatgattt ccttttttat
     5221 aagattattc atttttaaac ctcatgtttt gtgatatcta taatctgtgc tttaggtata
     5281 ttatttataa gttgttcatc atgagatata acaatgatat gcttttcctt gttttcctct
     5341 tttatataat cactgattat ttttatacca agtgaatcaa acccttttgt cggttcatcg
     5401 aaaataatca agtctacgtt ttttccagat gtaagagata ctcctaattt ttgtttttgt
     5461 ccaccagata aattatgtcc gtaacctttg atctcataat ctagtggttt gtcgaactta
     5521 aatctgtgta gcagcttttt aattgtattt aaattattaa tgccggaata atgtaatata
     5581 ttttcctcta tagttccagg catgagatct agatcttgag gaacgtataa gacattaaca
     5641 aaaggagaat ttgctttttc ttgttgtatc ccattgattt ttattgtgtt ttcttctgtt
     5701 gttatattgc cagagagaat ttttgcaata gttgtttttc ccgaaccatt ctcaccggtt
     5761 ataaaggtaa caacgccttt tgttaatatt aatttatggt tgaatgattt gttgtatatt
     5821 tcaatattgc aaatattgtc ttttacatga agatattgct tgtctgtgta atttgttttt
     5881 tttatgtcgt ttatttcggc acagtataaa tcaatttctg ttcttgccat ttttatttgt
     5941 ctaaatagat aaccaatgcg atttaatggg agaaaaactt ggaataaaaa agtggcaagc
     6001 ataataatac taccacggct tgctctacca tgaagaaaca tccagtatgc tgataggata
     6061 atcagactac taaaaatgac tgaaagaaaa gcggagctga taccaaacag gaaaaattca
     6121 ttatttactt ttctttgttt ttctgaatgt gtatttacag cttgaattac tcgtttacga
     6181 cttttatccg tggtatggaa agctttatcc ataggataca tgtgcatcat atcaaacatt
     6241 cttccagatg cgaatttgga gctaagtgca actgatttca tcattggaaa tctacgttgt
     6301 gtcattttta tgacaaatat tatatagatt gccattatta ttataaactc aatggctatt
     6361 aatgtattaa cacttataca aattgttatg attattccaa tcatttgtat taataatggt
     6421 actacatcag aacatattgt ataaaataat gatgatgcag aagaaagcat gctttctata
     6481 accttattta gttctccttt atttttcttt aaaaaatgct cttggtgaga attttgtata
     6541 ttatcaataa cttgctctct aagctttaca gataattcta tatttgtttt ttgagtcaag
     6601 tattccgata tgctatatcc agcaggcata aggaatctaa ttgataagaa acatgctata
     6661 aatattaata atgagtttat ttctcctatt gaatctttat tgtcaataac tgtttttatt
     6721 aaatatccga atagaacgaa aagaaaacta cttatagttg ataataatat aataaaaaac
     6781 aaattacatt tattaaatgt gatgatgtac ttttcaataa aattcaattt ataatcctta
     6841 aagcccgtat attacgggct ttatgttatc tagaattaca tgagcaccca gcgccacatc
     6901 ctttaccgct gcaaccacaa acagaaccat tatcattcca aacatctttt cgcattatta
     6961 tttttacaaa ttctgcggct ggaaggtgag tgatactctt tactttttcg atgtaagaat
     7021 cactggatat ggattctttt ggaattctta tagtattaat gttctttatg ctgtatctta
     7081 cagcataatc accataccac tttgcgacag tttcatgacc taggtaaaaa gcgatttttt
     7141 caatttgatt tttatattcg ttaaaacgat attcttggaa tgttatacca tgctcattaa
     7201 catgccttat ctcatttttt agcatgtcat ttgtatttgg ataatggtgg aggtaagcac
     7261 caagatattt ttcacaaaag ctggtataag catctgtaaa ttgaataaac gtatgccaca
     7321 tttcatctat aatttgtaat tgctcgtgaa cttttatatt gtagcattcc gttaggcatg
     7381 ttgaggctag ccagatgaat ttctttgttt cattaaagat atcatcagct tcttctggtg
     7441 ttacattcca ttctttacga aattttagaa gaacatcatc attgtgataa ctcatgatat
     7501 cttctaatga gctaacatta tgaacttttg cataagaact gctgttcatc ttatgttgta
     7561 gcataacacc cccaattata tatttgaatg tagatgagaa tagttatcat ttgttatggt
     7621 attgttaaat ttgtgcttgt gtcaatatgc ctaattctat aaatgagata gtaatcaagt
     7681 tttttaatgg ttataggatc cattagtaat acaaggtttt tgcagccagt ttttacgggt
     7741 aggggctgca aggaaaacaa aattttccca ctctaagaga agagaattga gttggttgaa
     7801 tgagtggatg catctagggc cgctaattca ccgagtgtaa aggctgtacg aaatgtgttg
     7861 cagtccgata atagccccct ccattgacgt tttacacatg tgttgtaggt tccaggagtg
     7921 ggcggatctc aaaaagatc
//
