LOCUS       NZ_CP023825             1552 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain 7/2 plasmid p7_2.5, complete sequence.
ACCESSION   NZ_CP023825
VERSION     NZ_CP023825.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07760941
            Assembly: GCF_003856675.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Sandegren,L.
  TITLE     Dynamics of resistance plasmids in extended spectrum
            beta-lactamase-producing Enterobacteriaceae in the gut microflora of
            patients during 1 year post UTI
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Sandegren,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2017) Medical Biochemistry and Microbiology,
            Uppsala University, Husargatan 3, Uppsala SE-75123, Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics workbench v. 10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20x
            Sequencing Technology             :: Pacific bio + Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003856675.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 02:46:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,203
            CDSs (total)                      :: 5,081
            Genes (coding)                    :: 4,903
            CDSs (with protein)               :: 4,903
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 65 of 178
            Pseudo Genes (incomplete)         :: 125 of 178
            Pseudo Genes (internal stop)      :: 37 of 178
            Pseudo Genes (multiple problems)  :: 42 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023825.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="7/2"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p7_2.5"
                     /geo_loc_name="Sweden"
                     /collection_date="2009"
     gene            484..960
                     /locus_tag="CRT46_RS26490"
                     /old_locus_tag="CRT46_27705"
     CDS             484..960
                     /locus_tag="CRT46_RS26490"
                     /old_locus_tag="CRT46_27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          2..87
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.954"
                     /coverage="95.0"
                     /evalue="4.39e-26"
     Typing          complement(297..405)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          complement(1191..1451)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.13"
                     /coverage="100.0"
                     /evalue="1.84e-104"
ORIGIN
        1 ggggtgtcgg ggcgaagccc tgaccagatg gcaaacggaa tatcgtcgcg ggtgacggta
       61 ttacatttgc acatcctgtc ccgatttctg cgggggttaa cggtgaacgg acggcaaaaa
      121 acgtgcagga gctgttgaac gtgaaaacca cgaaatctgg caaaaatcac ggctggcgag
      181 gcttgcagag gagtaagcca gtatacactc cgctatcgct acgtgactgg gtcagggctt
      241 cgccccgaca cccccaaagg gcgttgcgtt gcacgcaaca cccttgccta gaatagatct
      301 tttacgccga tttgtaagtt gttgtttttt tagtgtgttt tttgtttacc tgtcgcatcc
      361 agggtgacac aacgttgtcg catcctggat gcgacaacac gcaacaaggt gttgcacaga
      421 tgtgtcatga tggcttagat ttgaaccaac gttagtgcat gggattttta gagggaaaaa
      481 atcatgttta ttgattcaga aaaacgactg aaacaacttt cagatgaggc aaagaaaaac
      541 accgaggatc tcgaagaagc aaagaagaat tcaaggttta cacaggtatc cccaaaaggt
      601 tgggaacgcg ttcgagagct gctgaaggat agccaaggca tatcagcact gaagctgtac
      661 tcatttttag cggagcatat cgatcctacg tgtggcgctg tcgttgcgga tcagcaattc
      721 ctagctgaaa aacttggagt tagcagaagc acaattattc gttggctcaa ttacttagaa
      781 tcaaaaaatg cattagttag aatccccgtt gctggtaagg tttgtgcgta tgccctcgat
      841 ccacatgaag tctggaaggg atacaacact acgaaaaacc atgcagcatt tgtcactaaa
      901 acactggtca acaaagacgg tgatattcag cgccgaatca tggccatgtt ttcaaattga
      961 gccagcggca ggcggacaat caggggctac gtgttaacgt tctgaccatg attgtctatc
     1021 ctgcattgct cttttgccgc ctcaaaatcc cttgcgtgtt tttgctcccc gatctccaga
     1081 acgaccgagc cgccacggtt ccggcagcgc cttgagcgca accggaaacc gtctggggag
     1141 aaccccagac ccccatcgct gtaacaccga cggtctacga ccacctacca accgagtgac
     1201 tgcctgtacc gtgcaaatgg gctttgcaga gggctggcgc ggttcctggc gacctgaaag
     1261 cccctggcta ggggtatcgg cgcgcctgac ctcctgaaac gctctgtaag cgtttttcgc
     1321 gccttccatg ccctgacggg catttaaacc ccgcctcgtg cgtctgagag cttctgagcg
     1381 cgtttgaagg gctatctgac gactcatccc cgaaagaaca acagcggaat caccgacaac
     1441 atgcgagagg tggcaccgct cgccgcagcc ccagcgaccg aacgttagtg agcgagtggg
     1501 cgaggaagcg gaagagaacc ggaaaccaca ttgagcacgt acgcactgat ac
//
