LOCUS       NZ_CP023782            10647 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Enterococcus faecium strain Efaecium_ER03933.3A isolate isolate
            plasmid pER93933.3A.3, complete sequence.
ACCESSION   NZ_CP023782
VERSION     NZ_CP023782.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07679728
            Assembly: GCF_002848625.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10647)
  AUTHORS   Chako,K., Sullivan,M., Pak,T.J., Beckford,C., Altman,D., Ciferri,B.,
            Pak,T., Sebra,R., Kasarskis,A., Hamula,C. and van Bakel,H.
  TITLE     The genetic basis of emerging Vancomycin, Linezolid, and Daptomycin
            heteroresistance in a case of persistent Enterococcus faecium
            bacteremia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10647)
  AUTHORS   Chacko,K.I., Sullivan,M.J. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2017) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.2.0
            Expected Final Version            :: yes
            Genome Coverage                   :: 26.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/30/2023 13:51:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,955
            Genes (coding)                    :: 2,835
            CDSs (with protein)               :: 2,835
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 41 of 120
            Pseudo Genes (incomplete)         :: 68 of 120
            Pseudo Genes (internal stop)      :: 31 of 120
            Pseudo Genes (multiple problems)  :: 15 of 120
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023782.1.
            Bacteria and source DNA available from Icahn School of Medicine at
            Mount Sinai.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Efaecium_ER03933.3A"
                     /isolate="isolate"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pER93933.3A.3"
                     /geo_loc_name="USA: New York, NY"
                     /lat_lon="40.789990 N 73.953689 W"
                     /collection_date="2015"
                     /collected_by="Icahn School of Medicine at Mount Sinai"
     gene            1..933
                     /locus_tag="CO993_RS15915"
                     /old_locus_tag="CO993_03013"
     CDS             1..933
                     /locus_tag="CO993_RS15915"
                     /old_locus_tag="CO993_03013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="CO993_RS15920"
                     /old_locus_tag="CO993_03014"
     CDS             950..1420
                     /locus_tag="CO993_RS15920"
                     /old_locus_tag="CO993_03014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1821
                     /locus_tag="CO993_RS15925"
                     /old_locus_tag="CO993_03015"
     CDS             1504..1821
                     /locus_tag="CO993_RS15925"
                     /old_locus_tag="CO993_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_002302541.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRILL
                     F"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2111..3292
                     /locus_tag="CO993_RS15930"
                     /old_locus_tag="CO993_03016"
     CDS             2111..3292
                     /locus_tag="CO993_RS15930"
                     /old_locus_tag="CO993_03016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            complement(3521..4669)
                     /gene="tnpB"
                     /locus_tag="CO993_RS15935"
                     /old_locus_tag="CO993_03017"
     CDS             complement(3521..4669)
                     /gene="tnpB"
                     /locus_tag="CO993_RS15935"
                     /old_locus_tag="CO993_03017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(4686..5090)
                     /gene="tnpA"
                     /locus_tag="CO993_RS15940"
                     /old_locus_tag="CO993_03018"
     CDS             complement(4686..5090)
                     /gene="tnpA"
                     /locus_tag="CO993_RS15940"
                     /old_locus_tag="CO993_03018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            5719..6873
                     /locus_tag="CO993_RS15945"
                     /old_locus_tag="CO993_03019"
     CDS             5719..6873
                     /locus_tag="CO993_RS15945"
                     /old_locus_tag="CO993_03019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_002348708.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            6846..7289
                     /locus_tag="CO993_RS15950"
                     /old_locus_tag="CO993_03020"
     CDS             6846..7289
                     /locus_tag="CO993_RS15950"
                     /old_locus_tag="CO993_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            7591..7971
                     /locus_tag="CO993_RS15955"
                     /old_locus_tag="CO993_03021"
     CDS             7591..7971
                     /locus_tag="CO993_RS15955"
                     /old_locus_tag="CO993_03021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002348702.1"
                     /translation="MSEQNQNLASDHSKKYTYRSKPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7953..8867
                     /locus_tag="CO993_RS15960"
                     /old_locus_tag="CO993_03022"
     CDS             7953..8867
                     /locus_tag="CO993_RS15960"
                     /old_locus_tag="CO993_03022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002348701.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8827..9105
                     /locus_tag="CO993_RS16515"
     CDS             8827..9105
                     /locus_tag="CO993_RS16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            9107..9796
                     /locus_tag="CO993_RS15970"
                     /old_locus_tag="CO993_03023"
     CDS             9107..9796
                     /locus_tag="CO993_RS15970"
                     /old_locus_tag="CO993_03023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347611.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7954..8864
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatt atggtaaaaa atcatacaaa
     1741 aacactaata ctattatttc attttgtaat ttttttaagt ttagttttca ccttaattca
     1801 gtccggattc ttttatttta gccattcaat tgccacttgg ttattaatat tttttctaag
     1861 tttactatta ataattaata cattaataat taatacttta attattatct ttaataaaca
     1921 tagaaactta tttcttcaca gcatatttcc aattgtttta ttaatttttc tatactttat
     1981 ataaaaagat agaactattt agtaagataa aaaagtagct aattgtaatt ttcgtctaaa
     2041 actcaaaact agtaaaaata actttattgt gttattttta tttataaatt atgaaaaagg
     2101 acctttcgaa atgaaaaata atagaatcta caaaaatcag ttgaattatg ggcttttatt
     2161 gccagttttt ctgctatgta tcattggatt actttcttta tatgttgctt tatatcacga
     2221 ctcttacact aattcaaaaa tcacaaaaga attaattaaa caagtattgt ggtatagtat
     2281 tgggttcact tcagcaataa ctatttattt ttttaatcct aaaattttgt ggaaattaac
     2341 tcctatattt tacggattat gcatcataat gttattactt ctcttgaaat tctataatcc
     2401 acaattagca gtcttaactg gagcaaaaaa ttggttttat attgctggtt tttcgtttca
     2461 acctactgag ttagtcaaaa taggatacac ccttatgcta gctaaaataa ccgtcaatta
     2521 tcagcaaaaa tctattacta gtaatacgaa agatgattgg atttatttac ttaaaacttt
     2581 tattgctact cttccaatag gaatattatt attttttcag aacgatctag gaacaatgtt
     2641 agtcttcatt gttatttata tttttatttt gtttacgtct aacataaata taaaaataat
     2701 tgccccaata atcgccattg gtgttgtttt atccttaata tttttatatt tggttatatc
     2761 agatacagga agagatttct tattaaaaat aggatttcac gactatcagt ttaaaagaat
     2821 agactcatgg ttgaatccat ttttagatcc aaatggatct agctatcagt tagcaaatag
     2881 tttaattgct attggctctg gcggactttt aggtactggt tttaatgtat caaacattca
     2941 cgttcctgtt agagaatccg atatgatatt ttctgtaatt ggagaaaatt ttggctttat
     3001 aggaagttgt cttgtaatat tcctatactt ttatttaatt tatcaaatgt ataaaacatg
     3061 ttttaaaagc aataacttat ttttcaccta tatcggtata ggcattgttg caatgatttt
     3121 ttttcatgtt tttgaaaata taggtgcaag tatcggatta ctacctctaa ctggtattcc
     3181 actcccctat attagtcaag gtggttctgc cattcttagt aattttttgg ggttgggatt
     3241 tatgttgtgt tgtagtagat atactaacga aaaaaaagga acacaaaaat gaattcaaaa
     3301 taacacttta tgaatagaat gtctatttca ataaaaatta tgatacctta aagttaatca
     3361 gtgaactact caccacttag ctaaacctga cggttcttaa cgcttgaagt gggagcttct
     3421 tgggaataga gcatacttga tagcgtattt ctttactaac agcggtgtct cttatttacc
     3481 aagctatccc cgtagttcct acggttcttg atgattggat ctaagccaat cccatagaat
     3541 gtagtcttag accttcggtc agaatattga tactagcatt gatatcacga tcatgatgag
     3601 tatgacaaac aggacaagtc cattctcgaa tttcaagaga tttcttgcca tctttatgtc
     3661 cgcactctga acaaatctga ctagacggaa accatttatc tactttgatg atttttcgtc
     3721 cataccagtc agccttgtat tgtaatttag tcacaaactt tgaccaagaa acatcagaaa
     3781 tacttttagc taatttatga tttcgcaaca taccttttgt gtttaagtct tcaatacaga
     3841 taatatcgtg atttttgatc atttctgtac tcaacttatt cagaaaatca gtacgttgat
     3901 tcattacttt ttcatgcaat ctagctactt tgcgtttttg tttttgataa tttttagctt
     3961 caaatagatt ggtaccttta tttttagcca ataaagcacg cctagacaat ttgcgttgtt
     4021 cacgctttag tttcttttcc attttggacg tgaatttatt attatcaatt ttttgtccat
     4081 cagaaagaat cgcaaagtcc ataataccta agtcaatacc aattgtggaa ttagttttag
     4141 gtaattcgat aatttcttct ttacacaaca aagaaatata gtatttaccg ctagaatgac
     4201 gtgatattgt agcggatttg ataatccctt ttggttgtct atgaagctta attcttacta
     4261 atgacttcaa tttaggaact ttgatgaatt tactatcaat caaagcaact gtaccatttt
     4321 gattattcgt tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa
     4381 cggatttatc ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta
     4441 aagcaaggct atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg
     4501 gaaaagtcat ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat
     4561 caagcattag attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt
     4621 gttcctctgt tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct
     4681 actttctatt tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt
     4741 gcgccaccag ttgtcagaag acaaaaactt ttagaccaga acatttcttt ccaaagaaat
     4801 tgtttgactc ttggaaagtc acgttttatc aatcttgagc tggcactttt ataggcattg
     4861 atgaattttg tcaattctgt tttaggttga gctttgaaca taatatgaac gtggtcttta
     4921 tcatgattcc actccactaa agtaatgtga tacgattctg aaattctttc aaaagtagtt
     4981 ttggcataat cagatatttc atcatcaatc acttgtctac gatatttcgt tactaaaaca
     5041 aggtggtaat aaagaagaaa tactgaatga ttatttgtat ctaattccat gttatatcaa
     5101 ctcatttctt gtatagactg attgtaacat ggaacaaaat aaaaaggcaa tacgccttag
     5161 ttgtcggtct tcgagttacc aacggtaacc ctcataccga acggcattca tcacccacct
     5221 atagaggatg ggcgacttct gcctaattct gttaaattca aattattaca acagtcctaa
     5281 cgaagaattg tttgtaaaac aaacggataa ataataaaaa agactgctct ttcgagcagc
     5341 cagttaaaaa ataatgagtg tttattcttg aaaaagcaac gcgcttatga agcgctacca
     5401 aaaatttatc atattttatg aaaaataaca ccccagtaaa aaatgactgg taaaaattca
     5461 caaagaaacg gaaagaagca actgcagaaa atcaagcctt aaaaagagct tttgacctag
     5521 accaatgaaa aatcacaaag tgagcccctg tatcccttgg tagagaggct acttggcata
     5581 gtcgacatta tgttaactaa cgctataaat aacgattatg ttcccaaaac aatattttat
     5641 gaactataac aaaaatttgt tatcgcatat tgtatatttt aattataggt gtataccact
     5701 taaatttaac acaaaggcgt gattattttg aaacccaaaa agaaaattta tctaggtctc
     5761 ctcttttcct tgatgattgg aatttttagt ataggaatta caaataccta tgcttcagaa
     5821 aattctaatt tcaatatcaa ctcccaacca attgaagaac aagcaattaa ttttgcgaaa
     5881 actgattttg aacactccat aacttcaatt cttttaatgg aaaataacac cgaaactgat
     5941 attaactata ctcttggttc gccatttaaa atgctcgatg gagaaaatac ggcaaatagt
     6001 tctattttct ttccagtatt ttcagacgct gataatacga ttaaatatat ttataccgtc
     6061 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
     6121 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
     6181 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
     6241 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
     6301 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
     6361 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
     6421 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acatgctact
     6481 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
     6541 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
     6601 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
     6661 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
     6721 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
     6781 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
     6841 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
     6901 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
     6961 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
     7021 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     7081 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     7141 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     7201 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     7261 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     7321 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     7381 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgaa
     7441 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     7501 ccttttggtg agctttactc attgtgcctt tttaggtaca atggtattgc gaacgatacg
     7561 tccgtaggac gtactgaaag ggtgggactt atgagcgaac aaaatcaaaa tttggctagc
     7621 gatcattcta aaaaatatac ctatcgatcc aagccaaaac aaatcagttt tcgagtgagc
     7681 gaatctgaat ttgcaaagct gaagcagtca gctgaagctt tgcaaatgag tgtgccggct
     7741 ttcgtgaaag ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc gccagacatt
     7801 gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga atatcaatca aattgccaag
     7861 tggtgcaata cacaccaaca tgacgttgct cctggagacg cccaacgttt atccgaaaac
     7921 ctaaaaatca tgcaaaagga gctacaaaag atatggcaac agttaaagta agtcgaacca
     7981 cttcctgtag ccgagcaatc aattacgcag aaccacgagc aaccattaaa acaggaataa
     8041 actgtgacgt cgattatgcc aaaagtgaaa tgaaacaaat tcgcatgctg tatggaaaag
     8101 acgaccgtgt acaggcacac ttattgattc aatcttttcg accaggagaa attaccgctg
     8161 aaaaagctaa tcaactaggg gcagagtatg caaaaaaaat agcccccgat caccaaacag
     8221 ctatttacac acatacagac aaagaccata ttcataatca cattgtcatt aactccgtta
     8281 atctggagac tggaaaaaaa tttcaagctc atggtcaagc atttctaaac aaatgttatg
     8341 acctaaatga cgagatttgt cttgcacata acttaccaat cactgagcga ggaaagaaac
     8401 cagaaaaacg aaccatgtct gaaataaaat taaaagaaaa aaatgaacct gtttggaaag
     8461 acgaaattcg gattgcgatc gatcaaacaa tgaaaaacaa aaaaaccaga acctatgacc
     8521 aattttgtaa ttccctcaaa acattgggga ttcattgctt caatcgcggg aaaaatttca
     8581 cttacgaatt gatccacaaa aagaaaaaag tacgatccaa cagactagga aaggactacg
     8641 aaaaggagac gattttacat gagctggaca gacgagaacg aacctataac caacagcgaa
     8701 ccaacgagct acgaagagct cgagctagca ttcgcgaaac tgcagaagaa gtacgaagag
     8761 aacaacgaac tcttacgcct gaacaaccgg aaattagacc ggctcgaaga aaccatgaac
     8821 aacacgttga aagaggaaca gaccaccaac aagaattatc gctctaaaac gttaaaagac
     8881 atagaaaacg aaataaagaa acaacgtgct tttctcgttc cttatacgca agaaaggata
     8941 caaactacga ttgatttaca agttaaaaaa ctccatcaag agctattaga ccagcgaaat
     9001 aaggaacaga aaaaggtgct tattcaacga tcattggatt ttcttgcccc cttatgttgc
     9061 ttagtcctga cactgttcgc attttggtta gtatggggag gctaaaatgg cattttattt
     9121 accctatcta ctcatttttg ttagtatttc aggaagtatt tggctcatct ataaaatttt
     9181 tcaaacaaga tattcattaa aaggctctaa aattcgattt aagcgatttt tctttcttgg
     9241 ctgtatattt tccttgataa tcgtttcaag cggcttattc ggcgttttag agggaaataa
     9301 gcgcgtttct agaagtattt tattaggaaa tgtcactcaa aagtatgaat cagcgcgtga
     9361 taaaaagaaa aaagagcaag cattagcaca aaaaatggaa gagtttacca cttgttatga
     9421 ggaaatgaac gatatttttg tgaatcaaga aaaacgtttg acggataaaa acatggaaaa
     9481 actcactcgt ctttatcaaa acttgccaga aaaacagcaa aaagaggttc aagacaacta
     9541 tgagcgaacc aaaaaagacg tgcaatatgt gaaagatacg aaagtggaag aagcttgtag
     9601 tgatttattt ggagatacaa ttcctttttc aactagcgaa caagaacgaa aagaacgaca
     9661 acaatcagtc acatatgaac gctatgaaat cttgcttcaa caagcaatta acatacaaaa
     9721 tctgactaaa aaagaaacag ctttaaatta tttaaaaagc gtcaaggaat ggttagatca
     9781 gcaacagcaa aattgaattt gattgaaatt catgttaaac taaacatgca aatgttcaaa
     9841 cattagatac acgaaagccc cagagaggtc ggattctctg gggctttttt taggttgatg
     9901 tcgcctttac tgattttact tcttgtttcg gttattcaac caataactga atacagagat
     9961 acaaactcct acaagaaggg ggcaaataaa cagttcaaac atcagataca cctccttcca
    10021 gtcgcaagac tgtcggatag gcgacaagtt attataccct acaaaattaa atctgtgtgg
    10081 caattttaag tatctcttat ttccgggttg ttagggcttg ccctgaccgc ctgtagggcg
    10141 ctttaaattc cagataaagt tttttatact aagactgtct taaaacggct tagacaagct
    10201 cttagagcct caaccctcct ttttgaacaa tggttttagc aaaggagctg gaggcgactg
    10261 cgcaggtata atgtgagagc gtagcgatca cacctttttg gcgaccgtag ggagcgaggt
    10321 tttgaattag cagtttagaa aagttcgcga acctgtttaa agaaaaaata attgtttttt
    10381 taggtgggaa attttgtctc cctcttttat aatctttttt taaacctctt tttaaacctc
    10441 tttaacgtcg gctgtgtcct tactccccca aggtatacaa aattttatag gcatgaaatt
    10501 atctgtttat aggcatgaaa ttatctgttt ataggcatga aattatctgt ttataggcat
    10561 gaaattatct gttttaggta ttgtttttag acataatatg tcctaaaatg atgtcaataa
    10621 aatcatttag actggagtaa caactaa
//
