LOCUS       NZ_CP023649             7098 bp    DNA     circular CON 17-APR-2024
DEFINITION  Shigella sonnei strain CFSAN030807 plasmid pCFSAN030807_4, complete
            sequence.
ACCESSION   NZ_CP023649
VERSION     NZ_CP023649.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03612247
            Assembly: GCF_002442535.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Hoffmann,M., Sanchez,M., Baugher,J. and Timme,R.
  TITLE     Whole genome sequencing of cultured foodborne pathogen
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Hoffmann,M., Sanchez,M., Baugher,J. and Timme,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2017) CFSAN-ORS-DM-MMSB, US Food and Drug
            Administration, 5100 Paint Branch Parkway, College Park, MD 20740,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 700.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002442535.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 03:16:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,167
            CDSs (total)                      :: 5,037
            Genes (coding)                    :: 4,336
            CDSs (with protein)               :: 4,336
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 701
            CDSs (without protein)            :: 701
            Pseudo Genes (ambiguous residues) :: 0 of 701
            Pseudo Genes (frameshifted)       :: 197 of 701
            Pseudo Genes (incomplete)         :: 506 of 701
            Pseudo Genes (internal stop)      :: 123 of 701
            Pseudo Genes (multiple problems)  :: 117 of 701
            Pseudo Genes (short protein)      :: 3 of 701
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023649.1.
            Bacteria and source DNA available from Dwayne Roberson, US FDA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="CFSAN030807"
                     /serovar="Serotype group D"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="pCFSAN030807_4"
                     /geo_loc_name="Somalia"
     gene            219..1874
                     /locus_tag="AT852_RS27910"
                     /old_locus_tag="AT852_27910"
     CDS             219..1874
                     /locus_tag="AT852_RS27910"
                     /old_locus_tag="AT852_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_025672428.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSSAMESRKKKEDKKRNAENKLNEEKNKPRKGVK
                     DYGHDYHPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKGRKIYEWDSQHGELE
                     GYRASDGQHLGSFEPKTGNQLKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1884..2141
                     /locus_tag="AT852_RS27915"
                     /old_locus_tag="AT852_27915"
     CDS             1884..2141
                     /locus_tag="AT852_RS27915"
                     /old_locus_tag="AT852_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_025672427.1"
                     /translation="MGLKLHINWFDKTTEEFKGGEYSKDFGDDGSVIERLGMPLKDNIN
                     NGWFDVIAEWVPLLQPYFNHQIDISDNEYFVSFDYRDGDW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2308..2568
                     /locus_tag="AT852_RS27920"
                     /old_locus_tag="AT852_27920"
     CDS             2308..2568
                     /locus_tag="AT852_RS27920"
                     /old_locus_tag="AT852_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025672426.1"
                     /translation="MELKKSISDYTEAEFLELVKKICRAEGATEEDDNKLVREFKRLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            2630..2773
                     /locus_tag="AT852_RS27925"
                     /old_locus_tag="AT852_27925"
     CDS             2630..2773
                     /locus_tag="AT852_RS27925"
                     /old_locus_tag="AT852_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_021553018.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAELTGVE
                     TQ"
                     /name="colicin release lysis protein"
     gene            3302..4195
                     /locus_tag="AT852_RS27935"
                     /old_locus_tag="AT852_27935"
     CDS             3302..4195
                     /locus_tag="AT852_RS27935"
                     /old_locus_tag="AT852_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277133.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(4411..4686)
                     /locus_tag="AT852_RS27945"
                     /old_locus_tag="AT852_27945"
     CDS             complement(4411..4686)
                     /locus_tag="AT852_RS27945"
                     /old_locus_tag="AT852_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091753.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEMLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            4865..7084
                     /gene="mobQ"
                     /locus_tag="AT852_RS27950"
                     /old_locus_tag="AT852_27950"
     CDS             4865..7084
                     /gene="mobQ"
                     /locus_tag="AT852_RS27950"
                     /old_locus_tag="AT852_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_025672425.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     CGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPRSVHDPVNVYLSPGGAVMVTDIHAG
                     RPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSNDRRPVA
                     SPLPSAHRVDITADAAPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLMVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     BGC             1..6874
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4690..4864)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.17e-87"
     Typing          complement(4865..5223)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.889"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3303..4195
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.163"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4866..7081
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.647"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccccggaccc cgccaacaca tcacgggcca caaaattttt tgtggcccgc tctgcgtttt
       61 ctaagtgtta tccctcctga tttctaaaaa attttccacc tgaacttgac agaaaaaacg
      121 atgacgggta ctttttgacc tgtacataaa accagtggtt ttatgtacag tattaatcat
      181 gtaattaatt gttttaacgc ttaaaagagg gagtttttat gagcggtggc gatggacgcg
      241 gccataacac gggcgcgcat agcacaagtg gtaacattaa tggtggcccg accgggcttg
      301 gtgtaggtgg tggtgcttct gatggttccg ggtggagttc ggaaaataac ccgtggggtg
      361 gtggttccgg tagcggcatt cactggggtg gtggttccgg tcatggtaat ggcgggggga
      421 atggtaattc cggtggtggc tcgggaacag gcggtaatct gtcagcagta gctgcgccag
      481 tggcatttgg ttttccggca ctttccactc caggagctgg cggtctggcg gtcagtattt
      541 cagcgggagc attatcggca gctattgctg atattatggc tgccctgaaa ggaccgttta
      601 aatttggtct ttggggggtg gctttatatg gtgtattgcc atcacaaata gcgaaagatg
      661 accccaatat gatgtcaaag attgtgacgt cattacccgc agatgatatt actgaatcac
      721 ctgtcagttc attacctctc gataaggcaa cagtaaacgt aaatgttcgt gttgttgatg
      781 atgtaaaaga cgaacgacag aatatttcgg ttgtttcagg tgttccgatg agtgttccgg
      841 tggttgatgc aaaacctacc gaacgtccag gtgtttttac ggcatcaatt ccaggtgcac
      901 ctgttctgaa tatttcagtt aataacagta cgccagcagt acagacatta agcccaggtg
      961 ttacaaataa tactgataag gatgttcgcc cggcaggatt tactcagggt ggtaatacca
     1021 gggatgcagt tattcgattc ccgaaggaca gcggtcataa tgccgtatat gtttcagtga
     1081 gtgatgttct tagccctgac caggtaaaac aacgtcagga tgaagaaaat cgccgtcagc
     1141 aggaatggga tgctacgcat ccggttgaag cggctgagcg aaattatgaa cgcgcgcgtg
     1201 cagagctgaa tcaggcaaat gaagatgttg ccagaaatca ggagcgacag gctaaagctg
     1261 ttcaggttta taattcgcgt aaaagcgaac ttgatgcagc gaataaaact cttgctgatg
     1321 caatagctga aataaaacaa tttaatcgat ttgcacatga cccaatggct ggcggtcaca
     1381 gaatgtggca aatggccggg cttaaagctc agcgggcgca gacggatgta aataataagc
     1441 aggctgcatt tgatgctgct gcaaaagaga agtcagatgc tgatgctgca ttgagttctg
     1501 ctatggaaag caggaagaag aaagaagata agaaaaggaa cgctgaaaat aaattaaacg
     1561 aggaaaaaaa caagcctcgc aagggagtta aagattacgg tcatgattat catccagatc
     1621 ctaaaactga agatataaaa gggctgggtg agttaaaaga gggtaaacca aaaactccaa
     1681 agcaaggtgg tggcggtaaa cgtgctagat ggtatggaga taaagggcgt aagatttatg
     1741 agtgggactc tcagcatggt gagcttgagg ggtatcgtgc cagtgatggt cagcatcttg
     1801 gctcattcga gcctaagact ggtaatcagt tgaaaggacc tgatccaaaa cgaaatatca
     1861 aaaagtatct ttgagaggat gttatggggc ttaaattaca tattaattgg tttgataaga
     1921 cgaccgagga atttaaaggt ggtgagtatt caaaagattt tggagatgat ggctcggtca
     1981 ttgaacgtct tggaatgcct ttaaaagata atatcaataa tggttggttt gatgttatag
     2041 ctgaatgggt acctttgcta caaccatact ttaatcatca aattgatatt tccgataatg
     2101 agtattttgt ttcgtttgat tatcgtgatg gtgattggtg atcaaatatt atcagggatg
     2161 agttgatgta cgggcttcta gtgttcatgg atgaacgctg gagcctccaa atgtagaagt
     2221 gttatatttt ttattgagtt cttggttata attgctctgc aataatttaa ataggcatta
     2281 tttaaaacat tctcaggaga ggtgaaggtg gagctaaaga aaagtattag tgattatacc
     2341 gaggctgaat ttctggagct tgtaaaaaaa atatgtagag ctgaaggtgc tactgaagag
     2401 gatgacaata aattagtgag agagtttaag cgattaactg agcacccaga tggttcagat
     2461 ctgatttatt atcctcgcga tgacagggaa gatagtcctg aagggattgt caaggaaatt
     2521 aaagaatggc gagctgctaa cggtaagtca ggatttaaac agggctgaaa tatgaatgcc
     2581 ggttgtttaa ggatgaatga ctggcattct ttcacaacaa ggagtcgtta tgaaaaaaat
     2641 aacagggatt attttattgc ttcttgcagt cattattctg gctgcatgtc aggcaaacta
     2701 tatccgtgat gttcagggcg ggactgtatc accgtcgtca actgctgaac tgaccggagt
     2761 ggaaacgcag taacccgaaa tcctctttga caaaaacaaa gcgtgtcagg ctgattctga
     2821 tgcgcttttt tttgaaatgt cacaaaaatt ccatgtggga gatgggatct aaaatccccg
     2881 tgcagaactt tccatccagg gggagaaaac ttgtcgtttt gagccgttcg gtgttcagaa
     2941 cgcacgaaac cgatcgtgca tcgctttcgt gcatagttat gcaacgcgct aaaaacgatt
     3001 ctgacgcatt ttttatgttt accctggtgt ttccttgtct ttttgttttt tttgcgtcag
     3061 aaagcgactg agggtgtttt aaggggtgcg tacaacggga gttatggtaa atggatcggg
     3121 ttttcgggaa ggttcgacag gatttgccgt tgggtgtagt gtaagcgact gaaaaacaaa
     3181 cgccccgtaa atcgtgctct caccgccaag attgatcacg aaattacagg gcgccgggtt
     3241 ccgcgtttcc cgatgggaaa gcgcggttag ttaaactgtg taccgagaga aatcgtatca
     3301 catgagcgcc gtacttcaac gcttcaggga aaaattaccg cacaaaccgt actgtacgaa
     3361 cgatttcgcg tacggcgttc gcattctgcc gaaaaacatt gccattcttg cccgtttcat
     3421 ccagcagaac cagccacatg cactgtactg gcttcccttt gacgtggacc ggacgggggc
     3481 atcaatcgac tggagcgacc ggaattgtcc ggccccgaac atcaccgtaa aaaatccccg
     3541 taacgggcac gcgcatctgc tctacgcgct cgcccttcct gtgagaactg cgccggatgc
     3601 atcggcttcg gcgctcagat acgctgccgc tattgagcgt gcgttgtgtg aaaaactggg
     3661 cgcggatgtg aattacagcg gcctgatctg caaaaatccg tgccaccctg aatggcagga
     3721 agtggaatgg cgcgaggaac cctacactct cgacgaactg gctgattatc tcgatttgag
     3781 cgcctcagcg cgccgtagcg tcgataaaaa ttacgggctg gggcgaaact accatctgtt
     3841 cgaaaaggtc cgtaaatggg cctacagagc gattcgtcag ggctggcctg tattctcaca
     3901 atggcttgat gccgtgatcc agcgtgtcga aatgtacaac gcctcgcttc ccgttccgct
     3961 ttctccggct gaatgtcggg ctattggcaa gagcattgcg aaatatacac acaggaaatt
     4021 ctcaccagag ggattttccg ctgtacaggc cgctcgaggt cgcaagggcg gaactaaatc
     4081 taagcgcgca gcagttccta catcagcacg ttcgctgaag ccgtgggagg cattagggat
     4141 cagtcgagcg acgtactacc gaaaattaaa atgtgatcca gatctcgcaa aatgagacca
     4201 gataagcctt atcagataac agcgcccttt tggcgtcttt ttgagctgtg atctttcttg
     4261 cgattttctc cgttctgccc cgtaatggcg tggctttccg catgattgag gtggtagcgc
     4321 tcgccgcagt ctcatgaccg agcgtagcga gcgaatgagc gaggaagcgc aaaggcgtcc
     4381 ggtggtgcat gggcacttac gcgccggggc ttagtggttc tgcggtttcg cgggtggtct
     4441 gggtagcatc tccagctcgt taatcagcgg ttgtagtcgg ttcacatccg cctgtcttgt
     4501 gacttccttt cgcagaaact ggagcaggaa cgcacgcagt tgcgcttctt ccggcctccg
     4561 taccctcgcc agcatggcag cccccacaat gactttttgc gccgtgtcca ggctccggct
     4621 cttcgccttc aggcgctgta atctggcctc agcttcggca atcttctgtt cgagtgttct
     4681 gctcattttg tgactccatg cgcggtgaaa aatcgcattt tagcgcgtca ctggtagttg
     4741 caaaactgaa ctggcataat gcacggcaca tcacgaagtg cgcacttata caatctccac
     4801 ttcgtttcga ttgtgtgcgg tctgcgacgc taaaagaaaa cggcaaaaag gcattacggc
     4861 agaaatggcg atttatcatc tcagcatgaa aatcatttcg cgaaaaaacg gctacagtgc
     4921 cgttgcttct gctgcctacc gttccggctc tgtcataccc gatgaccgta ccggattaac
     4981 ccacgattac acccgtaaat gcggcgttga tgatgcggtc attctcaccc ctgcgaatgc
     5041 accgtcctgg tgtggtgacc gttccgttct ctggaatgcg gtcgagaaag ccgaacagcg
     5101 ccggaactcc cagctggcaa gggagattga acttgccatt ccccgtgaga tttcccgcga
     5161 ggccgcacgg gagaccgttc tcgcttttgt ccgggaaaac tttgtcagtc ggggcatgat
     5221 tgccgatgtg gcgttccatc acatggaccg gaccaatccc catgcgcaca tcatgctgac
     5281 cacgagagct gtcggggaaa cgggattcgc aggaaaggtc agggactgga acgaccgggc
     5341 actcgccgag acgtggcgcg catcatgggc tgaccatgcg aacagagcgc ttgcgaacgc
     5401 cggctaccag gaagagatag accatcgttc atacgagcgt cagggactgg agaaagcgcc
     5461 gggccttcac ctcggaaagg ctgcctgtgc gatggaaaaa cggggaatgg aaacagaacg
     5521 cggtgagcag aaccgtctga ttaacagcct taacctggaa atacaggttt cccgcacgca
     5581 gcttgctctc aggacggttc aggaagcgca gcgtaagcgg gaactcagcg atgctgcacg
     5641 tcgtgcagcg gaagccctta acctgaccat tcccgctgcg aatgcctcag cggataccct
     5701 gcgggaattc attgccacgc tgccgcagga atgcgggaac gcgtgggaga tgaccccgga
     5761 gttcctggcg atgagcggga aggtgaacga catcgaacgt gaggggaatg cgctgctgaa
     5821 agagcaggcc attctcgaaa aggagatgac cggactcaaa aaagcacgcc ctgtcgcgtc
     5881 ccttctgtca gagattcccc tgatgacatg ggctgaaccg gaataccgca aaagacaact
     5941 ccgtttctgg aaactcggga aacagattga atctcttcgc cgcacctaca gggccgtgaa
     6001 agaacgggac attcccgccc gtcgtcaggc ctttgaaacg cagtggaata cgtggattgc
     6061 gccgggaatg gcagagctga aagaaaaact gtcagcacgg gaagcggagc ggcgcaggga
     6121 ggaagccgaa gcggaagcgc gccggaagga acaggagcat gaggcgcggc tgaaacgtca
     6181 tgataaccac cgtctgagcc gtgaaacggc attagtcggg gttattacgg agctggggcg
     6241 tgccagagag ccgggaacgg gcaggataac ccgctacatg atgctgagta acagggccgg
     6301 agaattcacg gtatggggtg atgagctggc gcattacccc cggagtgttc atgacccggt
     6361 gaatgtttat ctgtcgcctg gcggggctgt gatggtcacg gatatacacg cagggcggcc
     6421 tgaatctcat gagacaatgg cgcggcctga gcgtgtgaga atgtattccg gtgcgacggt
     6481 ccggcatgta ctggaacaga tgcgccaggg gtggccctct tacggttttc cggcgctgcc
     6541 gcatcactgg ccggataatt tttatttcag caacgaccgc aggcccgtag cctctccgct
     6601 accgtcagcg caccgggtgg atatcaccgc tgatgcggca ccggaacaac tcatgcctgt
     6661 tgtattttcg agagagggaa acagcaggac gctgaatctg ttgttgtgca aagggccgga
     6721 ggaaatgctt gtcgggtttg ttcgccggga aggagggttt tgccccgttc ttgtgcttcc
     6781 gtcgccggat tacagtcatc tgatggtcag caccatcacg gagaacgggg tatgcctggc
     6841 aggttacgga gaagctataa accatgatga ggatactccg tacccaccgg aaccgcacct
     6901 gatgcagttc cggctcaaag gccatcatga caggcttttg gctgctgtcc acaaaccgga
     6961 agagatgccg gattatctct tccgtcaact cggttttaat cagacctggc atgagtggaa
     7021 gcgggacgaa cagcacaggc aacaacaacg ccgccccggg cacttccggg gcatgagtat
     7081 gtgatatccg gggctgca
//
