LOCUS       NZ_CP023647             8401 bp    DNA     circular CON 17-APR-2024
DEFINITION  Shigella sonnei strain CFSAN030807 plasmid pCFSAN030807_2, complete
            sequence.
ACCESSION   NZ_CP023647
VERSION     NZ_CP023647.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03612247
            Assembly: GCF_002442535.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Hoffmann,M., Sanchez,M., Baugher,J. and Timme,R.
  TITLE     Whole genome sequencing of cultured foodborne pathogen
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Hoffmann,M., Sanchez,M., Baugher,J. and Timme,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2017) CFSAN-ORS-DM-MMSB, US Food and Drug
            Administration, 5100 Paint Branch Parkway, College Park, MD 20740,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 700.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002442535.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 03:16:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,167
            CDSs (total)                      :: 5,037
            Genes (coding)                    :: 4,336
            CDSs (with protein)               :: 4,336
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 701
            CDSs (without protein)            :: 701
            Pseudo Genes (ambiguous residues) :: 0 of 701
            Pseudo Genes (frameshifted)       :: 197 of 701
            Pseudo Genes (incomplete)         :: 506 of 701
            Pseudo Genes (internal stop)      :: 123 of 701
            Pseudo Genes (multiple problems)  :: 117 of 701
            Pseudo Genes (short protein)      :: 3 of 701
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023647.1.
            Bacteria and source DNA available from Dwayne Roberson, US FDA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="CFSAN030807"
                     /serovar="Serotype group D"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="pCFSAN030807_2"
                     /geo_loc_name="Somalia"
     gene            complement(12..401)
                     /locus_tag="AT852_RS27830"
                     /old_locus_tag="AT852_27830"
     CDS             complement(12..401)
                     /locus_tag="AT852_RS27830"
                     /old_locus_tag="AT852_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            619..1059
                     /locus_tag="AT852_RS27835"
                     /old_locus_tag="AT852_27835"
     CDS             619..1059
                     /locus_tag="AT852_RS27835"
                     /old_locus_tag="AT852_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(1826..1975)
                     /locus_tag="AT852_RS29160"
     CDS             complement(1826..1975)
                     /locus_tag="AT852_RS29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            2546..2704
                     /locus_tag="AT852_RS29350"
                     /old_locus_tag="AT852_27845"
     CDS             2546..2704
                     /locus_tag="AT852_RS29350"
                     /old_locus_tag="AT852_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            3164..3979
                     /gene="sul2"
                     /locus_tag="AT852_RS27855"
                     /old_locus_tag="AT852_27855"
     ARG             3165..3976
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4040..4843
                     /gene="aph(3'')-Ib"
                     /locus_tag="AT852_RS27860"
                     /old_locus_tag="AT852_27860"
     ARG             4041..4840
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4843..5679
                     /locus_tag="AT852_RS27865"
                     /old_locus_tag="AT852_27865"
     CDS             4843..5679
                     /locus_tag="AT852_RS27865"
                     /old_locus_tag="AT852_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            5985..6227
                     /locus_tag="AT852_RS27875"
                     /old_locus_tag="AT852_27875"
     CDS             5985..6227
                     /locus_tag="AT852_RS27875"
                     /old_locus_tag="AT852_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(6259..6909)
                     /gene="tetR(A)"
                     /locus_tag="AT852_RS27880"
                     /old_locus_tag="AT852_27880"
     CDS             complement(6259..6909)
                     /gene="tetR(A)"
                     /locus_tag="AT852_RS27880"
                     /old_locus_tag="AT852_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            7015..8214
                     /gene="tet(A)"
                     /locus_tag="AT852_RS27885"
                     /old_locus_tag="AT852_27885"
     ARG             7016..8211
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<8181..8369)
                     /locus_tag="AT852_RS27890"
                     /old_locus_tag="AT852_27890"
                     /pseudo=""
     CDS             complement(<8181..8369)
                     /locus_tag="AT852_RS27890"
                     /old_locus_tag="AT852_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     ARG             4844..5676
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(2011..2184)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          2108..2713
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga
       61 cattgccagc cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg
      121 ttattgcgtg caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa
      181 ttaatattct tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat
      241 gcagtataag atgaaccact actattatca tggccaccat taccaccacc gttaaaacta
      301 cctcccgaac ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga
      361 ccaaaaccac cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact
      421 tttaaaaaca gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact
      481 acgcccgtca aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca
      541 gcctcagacc ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca
      601 ggagacactg tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga
      661 aagtcgcgct cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga
      721 aaaaatgatc gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc
      781 acttctgaga caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg
      841 gaaaatgctc agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc
      901 ctaatgcttg aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg
      961 gaacaactac aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg
     1021 ggattctggt cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt
     1081 accataacat ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac
     1141 cggtaaaaca ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta
     1201 cggcatgcat gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg
     1261 taagtcctgt ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg
     1321 cgtggccgtg aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg
     1381 gggtgatgta caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa
     1441 aagtgatgta taagtgatgt aaaagtgatg tataagtgat gtaaaagtga tgtatgaact
     1501 gaaggagcga tgtaattctg tagtcaggaa gaataacccg ttcatgcagg catgaacgat
     1561 acagtgagga acgctttttt tgtcacgatc tccccttaac ctgcgtaaat cacccgccat
     1621 ggttgattat ttttcaacct caaaatggtc atacacctaa cgcagccgcg attttcatcg
     1681 caactgcatg aattaaaaga aaaaaaagga atatttcgtt ttcacgagta atacccgggt
     1741 attaaaaatc cactgtaatt acatgaaaat aaagaaataa aaggtaatac cggagtatta
     1801 ccacgtacgc gggcaagcga aaaagctata acgcttcccg gccgacaggc gacccaaaat
     1861 ccacaggggc atgcaggttc tcttcagtaa cctgcgccat taaatcaaaa aggtcatatt
     1921 cgccgggaag ctgcgtatca gtcgaatcca gttgttcatc atattcagat ggcatggggt
     1981 gatatccagt agctgagagg ttgttagcgg gtttcggggc gcagccctga accagtcatg
     2041 tagcgctagc ggagtgtata ctggcttact atgttgccac gattgcgaaa atcagtgaag
     2101 tgcttcatgt ggcaggagaa aaaaggcagc accggcgcgt cagcagaata tgcgatacag
     2161 gagatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcggc tgcggcgagc
     2221 ggtaccggct tacggacggg gacggaaatt tcctggaaga taccaggaag acacttagca
     2281 aagaagagat aaggccacgg cgaagccgtt tttccatagg ctccgccccc ctgacaagca
     2341 tcgcaaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatccca
     2401 ggcgtttccc cctggtagct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg
     2461 tgtcattccg ctgttatggc cgcggtttct cattccacgc ctgacactca gttccgtgaa
     2521 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
     2581 cttgtccggt aactatcaac ttgagtccaa cccggaaaga cacgacaaaa cgccactggc
     2641 agcagccatt agtaacagga gtgcacgaga agatagatta agaggtcttg aagtagaggc
     2701 ctgactgcgg ctacactgaa agaacagttt tggtaatggc ggtctcgtga accctgttac
     2761 cgggttaagc agttccccaa ctgacttaac cttcgaacaa accgcctccc caggcggttt
     2821 tttcgtttac agagcaagag attacgattc attgaaaagg atctcaagaa gatcctttgt
     2881 agattactat caattaatct tccgaacatg acaccagtca ctgttgatta cggttaaacc
     2941 gctccgtaac aggtttccct cgtattggtg acttgcagca ccaaagaagc aaaaaagata
     3001 aatatatcta cttattgaga gatgatatat ttatttccaa aatgctacag atacatcatc
     3061 atgaaacgga tcaccgcaaa caggttactc gcctggggat tccctttcga cccgagcatc
     3121 cgtatgagac tcatgctcga ttattattat tatagaagcc cccatgaata aatcgctcat
     3181 cattttcggc atcgtcaaca taacctcgga cagtttctcc gatggaggcc ggtatctggc
     3241 gccagacgca gccattgcgc aggcgcgtaa gctgatggcc gagggggcag atgtgatcga
     3301 cctcggtccg gcatccagca atcccgacgc cgcgcctgtt tcgtccgaca cagaaatcgc
     3361 gcgtatcgcg ccggtgctgg acgcgctcaa ggcagatggc attcccgtct cgctcgacag
     3421 ttatcaaccc gcgacgcaag cctatgcctt gtcgcgtggt gtggcctatc tcaatgatat
     3481 tcgcggtttt ccagacgctg cgttctatcc gcaattggcg aaatcatctg ccaaactcgt
     3541 cgttatgcat tcggtgcaag acgggcaggc agatcggcgc gaggcacccg ctggcgacat
     3601 catggatcac attgcggcgt tctttgacgc gcgcatcgcg gcgctgacgg gtgccggtat
     3661 caaacgcaac cgccttgtcc ttgatcccgg catggggttt tttctggggg ctgctcccga
     3721 aacctcgctc tcggtgctgg cgcggttcga tgaattgcgg ctgcgcttcg atttgccggt
     3781 gcttctgtct gtttcgcgca aatcctttct gcgcgcgctc acaggccgtg gtccggggga
     3841 tgtcggggcc gcgacactcg ctgcagagct tgccgccgcc gcaggtggag ctgacttcat
     3901 ccgcacacac gagccgcgcc ccttgcgcga cgggctggcg gtattggcgg cgctgaaaga
     3961 aaccgcaaga attcgttaac tgcacattcg ggatatttct ctatattcgc gcttcatcag
     4021 aaaactgaag gaacctccat tgaatcgaac taatattttt tttggtgaat cgcattctga
     4081 ctggttgcct gtcagaggcg gagaatctgg tgattttgtt tttcgacgtg gtgacgggca
     4141 tgccttcgcg aaaatcgcac ctgcttcccg ccgcggtgag ctcgctggag agcgtgaccg
     4201 cctcatttgg ctcaaaggtc gaggtgtggc ttgccccgag gtcatcaact ggcaggagga
     4261 acaggagggt gcatgcttgg tgataacggc aattccggga gtaccggcgg ctgatctgtc
     4321 tggagcggat ttgctcaaag cgtggccgtc aatggggcag caacttggcg ctgttcacag
     4381 cctatcggtt gatcaatgtc cgtttgagcg caggctgtcg cgaatgttcg gacgcgccgt
     4441 tgatgtggtg tcccgcaatg ccgtcaatcc cgacttctta ccggacgagg acaagagtac
     4501 gccgcagctc gatcttttgg ctcgtgtcga acgagagcta ccggtgcggc tcgaccaaga
     4561 gcgcaccgat atggttgttt gccatggtga tccctgcatg ccgaacttca tggtggaccc
     4621 taaaactctt caatgcacgg gtctgatcga ccttgggcgg ctcggaacag cagatcgcta
     4681 tgccgatttg gcactcatga ttgctaacgc cgaagagaac tgggcagcgc cagatgaagc
     4741 agagcgcgcc ttcgctgtcc tattcaatgt attggggatc gaagcccccg accgcgaacg
     4801 ccttgccttc tatctgcgat tggaccctct gacttggggt tgatgttcat gccgcctgtt
     4861 tttcctgctc attggcacgt ttcgcaacct gttctcattg cggacacctt ttccagcctc
     4921 gtttggaaag tttcattgcc agacgggact cctgcaatcg tcaagggatt gaaacctata
     4981 gaagacattg ctgatgaact gcgcggggcc gactatctgg tatggcgcaa tgggagggga
     5041 gcagtccggt tgctcggtcg tgagaacaat ctgatgttgc tcgaatatgc cggggagcga
     5101 atgctctctc acatcgttgc cgagcacggc gactaccagg cgaccgaaat tgcagcggaa
     5161 ctaatggcga agctgtatgc cgcatctgag gaacccctgc cttctgccct tctcccgatc
     5221 cgggatcgct ttgcagcttt gtttcagcgg gcgcgcgatg atcaaaacgc aggttgtcaa
     5281 actgactacg tccacgcggc gattatagcc gatcaaatga tgagcaatgc ctcggaactg
     5341 cgtgggctac atggcgatct gcatcatgaa aacatcatgt tctccagtcg cggctggctg
     5401 gtgatagatc ccgtcggtct ggtcggtgaa gtgggctttg gcgccgccaa tatgttctac
     5461 gatccggctg acagagacga cctttgtctc gatcctagac gcattgcaca gatggcggac
     5521 gcattctctc gtgcgctgga cgtcgatccg cgtcgcctgc tcgaccaggc gtacgcttat
     5581 gggtgccttt ccgcagcttg gaacgcggat ggagaagagg agcaacgcga tctagctatc
     5641 gcggccgcga tcaagcaggt gcgacagacg tcatactaga tatcaagcga cttctcctat
     5701 cccctgggaa cacatcaatc tcaccggaga atatcgctgg ccaaagcctt agcgtaggat
     5761 tccgcccctt cccgcaaacg acccctggaa gccccgtagc gacgcggaga ggggcgagac
     5821 aagccaaggg cgcaggctcg atgcgcagca cgacatagcc ggttctcgca aggacgagaa
     5881 tttccctgcg gtgcccctca agtgtcaatg aaagtttcca acgcgagcca ttcgcgagag
     5941 ccttgagtcc acgctagatc tatctcatct gcgcaaggca gaacgtgaag acggccgccc
     6001 tggacctcgc ccgcgagcgc caggcgcacg aggccggcgc gcggacccgc gccacggccc
     6061 acgagcggac gccgcagcag gagcgccaga aggccgccag agaggccgag cgcggccgtg
     6121 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     6181 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     6241 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     6301 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     6361 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     6421 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     6481 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     6541 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     6601 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     6661 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     6721 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     6781 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     6841 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     6901 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     6961 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     7021 cccaacagac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     7081 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     7141 gcccactatg gcattctgct ggcgctgtat gcgttgatgc aatttgcctg cgcacctgtg
     7201 ctgggcgcgc tgtcggatcg tttcgggcgg cggccggtct tgctcgtctc gctggccggc
     7261 gctgctgtcg actacgccat catggcgacg gcgcctttcc tttgggttct ctatatcggg
     7321 cggatcgtgg ccggcatcac cggggcgact ggggcggtag ccggcgctta tattgccgat
     7381 atcactgatg gcgatgagcg cgcgcggcac ttcggcttca tgagcgcctg tttcgggttc
     7441 gggatggtcg cgggacctgt gctcggtggg ctgatgggcg gtttctcccc ccacgctccg
     7501 ttcttcgccg cggcagcctt gaacggcctc aatttcctga cgggctgttt ccttttgccg
     7561 gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg ctctcaaccc gctcgcttcg
     7621 ttccggtggg cccggggcat gaccgtcgtc gccgccctga tggcggtctt cttcatcatg
     7681 caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt tcggcgagga tcgctttcac
     7741 tgggacgcga ccacgatcgg catttcgctt gccgcatttg gcattctgca ttcactcgcc
     7801 caggcaatga tcaccggccc tgtagccgcc cggctcggcg aaaggcgggc actcatgctc
     7861 ggaatgattg ccgacggcac aggctacatc ctgcttgcct tcgcgacacg gggatggatg
     7921 gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg gaatgccggc gctgcaagca
     7981 atgttgtcca ggcaggtgga tgaggaacgt caggggcagc tgcaaggctc actggcggcg
     8041 ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca cggcgatcta tgcggcttct
     8101 ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg ccctctactt gctctgcctg
     8161 ccggcgctgc gtcgcgggct ttggagaaat tcttcaaatt cccgttgcac atagcccggc
     8221 aattcctttc cctgctctgc cataagcgca gcgaatgccg ggtaatactc gtcaacgatc
     8281 tgatagagaa gggtttgctc gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg
     8341 ctggccgtcc tggccgccac atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat
     8401 a
//
