LOCUS       NZ_CP023532            13833 bp    DNA     circular CON 14-JUN-2024
DEFINITION  Escherichia coli strain FDAARGOS_403 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP023532
VERSION     NZ_CP023532.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07312447
            Assembly: GCF_002393365.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13833)
  AUTHORS   Minogue,T., Wolcott,M., Wasieloski,L., Aguilar,W., Moore,D.,
            Tallon,L., Sadzewicz,L., Ott,S., Zhao,X., Nagaraj,S., Vavikolanu,K.,
            Aluvathingal,J., Nadendla,S. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13833)
  AUTHORS   Minogue,T., Wolcott,M., Wasieloski,L., Aguilar,W., Moore,D.,
            Tallon,L., Sadzewicz,L., Ott,S., Zhao,X., Nagaraj,S., Vavikolanu,K.,
            Aluvathingal,J., Nadendla,S. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 2.3.0, HGAP v. 3
            Genome Coverage                   :: 828.249x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002393365.1-RS_2024_06_14
            Annotation Date                   :: 06/14/2024 03:23:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,418
            CDSs (total)                      :: 5,292
            Genes (coding)                    :: 4,991
            CDSs (with protein)               :: 4,991
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 128 of 301
            Pseudo Genes (incomplete)         :: 192 of 301
            Pseudo Genes (internal stop)      :: 61 of 301
            Pseudo Genes (multiple problems)  :: 70 of 301
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023532.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_403"
                     /serotype="O104:H4"
                     /isolation_source="human"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_403"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /collected_by="USAMRIID < Columbia University"
     gene            complement(<2..3230)
                     /locus_tag="CO706_RS29945"
                     /old_locus_tag="CO706_00005"
                     /pseudo=""
     CDS             complement(<2..3230)
                     /locus_tag="CO706_RS29945"
                     /old_locus_tag="CO706_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /name="LPD7 domain-containing protein"
     gene            complement(3320..3870)
                     /locus_tag="CO706_RS00020"
                     /old_locus_tag="CO706_00020"
                     /pseudo=""
     CDS             complement(3320..3870)
                     /locus_tag="CO706_RS00020"
                     /old_locus_tag="CO706_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /name="phospholipase D family protein"
     gene            complement(3885..4175)
                     /locus_tag="CO706_RS00025"
                     /old_locus_tag="CO706_00025"
     CDS             complement(3885..4175)
                     /locus_tag="CO706_RS00025"
                     /old_locus_tag="CO706_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001314267.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSETTFWICTGI
                     ILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
                     /name="hypothetical protein"
     gene            complement(<4186..5316)
                     /gene="traJ"
                     /locus_tag="CO706_RS00030"
                     /old_locus_tag="CO706_00030"
                     /pseudo=""
     CDS             complement(<4186..5316)
                     /gene="traJ"
                     /locus_tag="CO706_RS00030"
                     /old_locus_tag="CO706_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /name="traJ"
     gene            complement(5313..6129)
                     /gene="traI"
                     /locus_tag="CO706_RS00035"
                     /old_locus_tag="CO706_00035"
                     /pseudo=""
     CDS             complement(5313..6129)
                     /gene="traI"
                     /locus_tag="CO706_RS00035"
                     /old_locus_tag="CO706_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /name="traI"
     gene            complement(6126..6584)
                     /gene="traH"
                     /locus_tag="CO706_RS00040"
                     /old_locus_tag="CO706_00040"
     CDS             complement(6126..6584)
                     /gene="traH"
                     /locus_tag="CO706_RS00040"
                     /old_locus_tag="CO706_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="WP_001079808.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQY
                     TLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVRLP
                     LPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
                     /name="traH"
     gene            complement(6979..7563)
                     /locus_tag="CO706_RS00045"
                     /old_locus_tag="CO706_00045"
     CDS             complement(6979..7563)
                     /locus_tag="CO706_RS00045"
                     /old_locus_tag="CO706_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="WP_000977522.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEINA
                     DNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNAVR
                     TIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLARDRL
                     GKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
                     /name="histidine phosphatase family protein"
     gene            complement(7622..8824)
                     /locus_tag="CO706_RS00050"
                     /old_locus_tag="CO706_00050"
     CDS             complement(7622..8824)
                     /locus_tag="CO706_RS00050"
                     /old_locus_tag="CO706_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="WP_000976357.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASSH
                     YGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPNDVSNKADDVKDDWDLFDSAVDNQ
                     HGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGKVND
                     ADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGVTPKY
                     QRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQNIIPRS
                     IDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDVDLTPASGFTSDSKRQFASVGAEF
                     NAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGAMAQLQFT
                     F"
                     /name="conjugal transfer protein TraF"
     gene            complement(8910..9734)
                     /gene="traE"
                     /locus_tag="CO706_RS00055"
                     /old_locus_tag="CO706_00055"
     CDS             complement(8910..9734)
                     /gene="traE"
                     /locus_tag="CO706_RS00055"
                     /old_locus_tag="CO706_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="WP_001238932.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGVR
                     EVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPLLA
                     TLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEWPDT
                     ADNFRMLINDSSNTEVIFWHSFPFAGNTKREYCGVILRQEKEINGSVIYPALSLSPTLL
                     NDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAENLITGQILPVSLFNTSLLPW"
                     /name="traE"
     gene            complement(9885..11037)
                     /locus_tag="CO706_RS00060"
                     /old_locus_tag="CO706_00060"
                     /pseudo=""
     CDS             complement(9885..11037)
                     /locus_tag="CO706_RS00060"
                     /old_locus_tag="CO706_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /name="site-specific integrase"
     gene            11036..11443
                     /locus_tag="CO706_RS30105"
     CDS             11036..11443
                     /locus_tag="CO706_RS30105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003552681.1"
                     /translation="MLTSKEAKNNANNHSLQACQSGTWKTSGSLNGSYTNLGSHRGSFS
                     GRNSGGSTLFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGKTAFISFA
                     VPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
                     /name="hypothetical protein"
     gene            complement(11440..>11664)
                     /locus_tag="CO706_RS30575"
                     /old_locus_tag="CO706_00075"
                     /pseudo=""
     CDS             complement(11440..>11664)
                     /locus_tag="CO706_RS30575"
                     /old_locus_tag="CO706_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            13077..>13832
                     /locus_tag="CO706_RS00105"
                     /old_locus_tag="CO706_00105"
                     /pseudo=""
     CDS             13077..>13832
                     /locus_tag="CO706_RS00105"
                     /old_locus_tag="CO706_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /name="site-specific integrase"
     Typing          complement(5317..5681)
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.19e-145"
     Typing          complement(5933..6129)
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="96.97"
                     /coverage="85.0"
                     /evalue="1.18e-98"
ORIGIN
        1 cgataaacag atgtgagccg acgttttctg catacgtaac gctttcatct cttcgggcca
       61 cccctggtga tttgcgcagc cactttcata aaggtttcga ccgggaatcg ctgaatacgc
      121 tgacctttat ccggattatc atgtgatacc gagatcccgt tggtaactga ctccatggac
      181 cacttattcc ggaaacgtgc tttttgctct cccgggagga tgaattgttc gcggttctga
      241 agtgtcagtg tcaccacgtc acctttctga tggtttttga ccagactttc cagttgttcc
      301 ccccagtaga gtttgctgcc actgcggttt ttaagctcaa caaagaaact ttctcctttg
      361 ccgggtgcat attcagcacg accaaaattg tgcagtacgg cagtcacgct ctctcccggg
      421 cgaagttttc ctggtggttc ttcacctggt gctgcatctg ccgttttgct ggttcagtcg
      481 gtgatgatgc aggagcggtg ctgacggttg atgattcagt ggcagcaggg gacgccgggg
      541 atgtgtggct tctttttcct gaacggggtc agggacagct ggttgttcag gggtattccg
      601 gttaagttcc ggtgttggct gatgggtgac aacagcatcc ttactgacag ccatttcctt
      661 gcgtaatttt tctagttcag cccgttgatc tggcaatttc atccggacat cgatgttgtg
      721 ctcgataatc aactgcattg ctttttgctt aaaggcatca ctgcccgtca gctctatgac
      781 gccccataaa tttggtggcc acagcaagtg cggccagaac gctgcgatcg tcattgctgg
      841 caccgttgac catttccagc cggtttaccg gtcaatgaag gcaggtttgc catccagttt
      901 gtacaggacg gttctgtctg ctcgttttcc cagtcaagct ctttaagaat ttcgtccatg
      961 ttgagcgata tcggtgcatc agaacgttcc ggggcgtaga caatactgtc aggtatcggt
     1021 ggagtcgtcg tggtttccgc tgagggcgtg ggatcactgt tctcatgcgc ctgtgaatcc
     1081 gtttttttac gcgtaaaaag gccacgcgct ttgtcgagaa acgatgaagt attatcagaa
     1141 ggtgatgtgc tctgaacagg ttgctgcgga gtgggttcat cccggacagc cggttctata
     1201 gtggctgttg tggcctgaag ttctgactca tctgcctgaa cgtggcctgt gtccggttgc
     1261 gacggcatat cactgttttc ctgtagatgc tcaggatttt cattgttcat cagctcctga
     1321 tatgcgctgt agtcttcgta ttcttcaggg ccatctgact caggaaggaa cggttctggc
     1381 tgaaggggaa tgtcttcagt tacatggctg accgcctctt ccggggagta tccggagata
     1441 ttccggtgct ttgtttgtca tcaggctccg tcatcacggt catttcctct ttgactgcat
     1501 caggggaagg tgatggttcg gttgattcat tcatggtttc agatgagggg ctgtcttcag
     1561 ggcggcgggg cagcaggggc cggtatttct ggtgaagtgc cggactcctg ttgtaactcg
     1621 tttttttccg gttgtgacag ttcggcttca aattcacgcc agtctttatc tgtggccaca
     1681 gagacaactt tttccggagg ttccgccgga tgagcgagtt catcaggggc agacgccgtt
     1741 gtactttccg gggatgccgg tgattctgca ggggcgtcct gaactacaga gctttccgtt
     1801 tgcgcctgaa cgttatcagg gtgccgctca tctttgccgt tattttcagt ggcgtgactg
     1861 gtaaatggct gtgtttcatc agcaatgtta ccgctggaag cagcaggttg tacctgctgc
     1921 gtggcggacg gcagagaagg tccctggtca tggctgtgcc ctgtatcggg tgatgtttca
     1981 ggatattctg tcggagtcat ggttttttcc tcagtgtgga ccggtgtaaa aacgaggttg
     2041 ccgatcacca gtgcctcccg gatttccggg gtgttactgt tgggggtgga aaccccgata
     2101 tgttctaact gggcgttgat gacatgcaga aaagcggccc gtccccggct ggcatccagg
     2161 tcattgaaat cggtcagccc ctgcgctttt tcggcatcgg tgaacgcagg aaagatgacc
     2221 gatcctcctg tcagttctgc ggcttttgtt gcactgacga tacctttgtt aacctggcgt
     2281 gcatgatcgt tgtcggcaca gaagataaag ggctttgctt ccatttttgc cgggcctctt
     2341 cggccacaat aatcatgttg cccgcatcca ctgtcatcag taccggaagg cctgtggctt
     2401 cgtgcaggga ggcggcagtg gcgtagccct cggcaaacag taccggctgg ccgttgcgcg
     2461 gcgtgcccag cgcaaaccag ttaccggttt tctcggaatc tttcaggatg cgggcatcct
     2521 tcccgccggt gaccggaatg cgctggtagg agcgtattgc gccgttacgg ttgctgaaag
     2581 gaataacaag ctcatttttg tcgtttattc gcacccccgg cgcagcctga atgcctttcc
     2641 gggtcaggta ttcatgtgcc gtggcctgcg gccatttgct gacatagcgg cgggcatagg
     2701 cagcctgtcg gttgtactgt gctttcagtt cccgctccgc gtcttccctg cgttgcatcg
     2761 aatgcgcttt caggtgaagt cttgcgcggg gatctgtctg ctctccgccg gagaaagtcc
     2821 aggtgaccgg cgaatcatcc gcgctgcggt aatccctgta ccagccggca ggtctgccgt
     2881 ccaggaagcc gcgatatgcg ccactttttt gtcctttttg tcaccggcgg tcgggacacg
     2941 gtggattttc ccgtccatga ccggcagttc tttcagaacc agcccggcat tttccagtac
     3001 ctgggcaaac tcggtgaccg ggtcactgcc gttcatggag acttcctgcg gccggggcag
     3061 ccagcggtcc agcatggaca ggtccgcccc cgggcgggca aaccagagtt tgtggtcctt
     3121 gtcccagaga acgggcgtgt gcccgttcat cgggggatgt gctttttgag atcttcgatt
     3181 tcatctgcag gcacggcaag ccaggtgcgg tatcgggata cagacggcat gagtgcttct
     3241 cctgaaaaaa gacggaaaaa aaccaccgcc ggaaaccggg ggtggctggg gggatagaaa
     3301 accgggggag aaatgtttgt cagggtctcc agtctgtacc cttattccag cgatcctgcc
     3361 agtgttcaag atatgtgcgg ctacatccgg catattctta agcacaacga cattctctga
     3421 attcttgcgt gcagcagcac gagagaagtt ataggagccg gtttcaaccg tgtttccgtc
     3481 aacaatgatg actttgtcgt gatggatggg gaagacatct acagtccgca gaggaatgtc
     3541 cagcaggttg atatatttca tcgcttcccg acttgcccgg ttcgtattcc ccttgtcatc
     3601 aacaacaata cggacgtcaa ctccgcgttt tttcgctttt gccagcgcat gcattacatc
     3661 cgggtcggtg aaagaatatg ccatcatcct gatggagctt tccgcactgt tgattgtgtc
     3721 aagcaccaga gcggatgcac ttccttcagg agaaaagccg accttcacgg atggcgtgtc
     3781 gatcattgca aacgatggtg tcgtgaacag taacgggcat gccagtagtg atacccggag
     3841 cagagtaacg atagtttttt tacctgacat aaagactcca gaatttaatc gaggccagtc
     3901 cagtctcgtg gtgattccgt atggtggcga taccaccatg ggcgaccgga ggcgatggcc
     3961 cctctcagaa tgctgaaaat acgtgtacag atagtcctga cagtccagcc tgaacggtcg
     4021 agaatgacga aaaataaaat gatgccggtg caaatccaga aggttgtttc ggagggaaaa
     4081 gggcatataa acagataaat catggtgaac ggcagaggta cgccctggat gttgactgga
     4141 ggtgttgcat atcgccagaa accttctctt ttcatgaccg taactccctg gcgggataaa
     4201 aagaatcgct tctcttcggt caacttcatt ccggatatac atttccagaa tctgatcccg
     4261 gatacgacgt ttttcctgcc ggattatggc atcaatgtga tgcccccact ctgcatgtgg
     4321 cattccggaa agattgtcgc gaagctcatc gttaatgacg atatattccc ggacggcttt
     4381 acggccaccg tcatttgttc tgaccagaac ctgaaccaga atgaactgca acagtgacag
     4441 gacagcccag gccatcgaat ccctgatgac agggggaaac agcccaagaa gacgtgccag
     4501 cgtttcacca ggagatttcg tatgcatggt gctcaggcag taatgtccgg tgttgcctgc
     4561 ctgtaccgct gcgtcggcgg tttcgttatc cctgatctca ccaacaccga taattcggga
     4621 ttacgtcgta cagcagaacg cagtccggca gcaaaactga ccacatcacg cccgatttct
     4681 gcctgatgcg gtgggagcag atcgttttca tttcccagat atattccacc gggtcttcat
     4741 atgtcacgat tttggcgtcg gggaaattgt ccataatgta acggtacagt gctgaacaca
     4801 gcgttgattt tcctgacccg gtttcaccac atacaaatcc cagaccgctt ttgcagacca
     4861 ttgcttcaag caggtctggt tcaatgttca tggagaggat gtccggtatt tctgtcggga
     4921 tgacacgcat ggttattgat attgctttct cctccgcacc agatgtgccc tgaatgaggt
     4981 gagagcgtaa ccggatgcgt tctccgcgtt taagaccata gcgaccggat gcatccccgt
     5041 taatctgtat cgtcctgtca accggattgc ctgccagaac gcgggaatga cttcccgacc
     5101 gaacagttca tcaatcaggg aggacatgag tgtggttggt aacgttgcac tggaacaacg
     5161 cacccgtcgg ccaaagcgac tgacagatac aggtgaccca ccggtcagat ccacatcact
     5221 gactttatgt gcggcacacc agacaaagaa atgccgtagt tcatctgcag tgaaacgctg
     5281 aaacggaaaa agaccaaact catcaggaat attcatcggg agcctccttc agtgcctata
     5341 agcggacgcc agtgagattt ctgaagattg aattccgcat gatttttaag gcgacgaaca
     5401 cgatctccag tgaccagttt tttcccgtca cctgtaacgg tttgcatttg atcagaaaca
     5461 tccggacggg tgatcatgcc ctgacgccag agaagtgagt acagcatcat gccccggtaa
     5521 tcacgggtga gggttttctg gttggcttca agcgtcaggt cggcgttttg tcttccttcc
     5581 tgccatccct gccgtaatgc ggtttcccac acttttcgtt gcacgctgtc ttctggaacg
     5641 acgctgcctt ctgtacccgg agtggctgtt gttgaaagcc ccggagaagc cagtcacgcc
     5701 agcccggtgg attgctgaca aagcgttcag ggcgaatgat ggtccagacc cgggaagagg
     5761 tccggatttg atctggcgtg atatgagcta cgtcctgtgc ttcatcaata accggtggta
     5821 gccagccttc cgggctaatc agtggccgga aatcgtataa ggcattaagt gtgctttccc
     5881 gggcgttgag tgcctgaatc agctcccatg agcgctgagc cttgccacca cgaaatccaa
     5941 gcgtttacct gcgtcggtca gcattttcca gcgtgtttct gacagactat ccggcttttc
     6001 acttcgtggt ttaagccatg cattaatgtc aggtggtggc gtattgacac cacctggcga
     6061 gatggtaggg gaagaagaac aaccagtaag cagtattact ggcaaaagga gagaggtgag
     6121 tgctttcatt ttttccggtt ctccggctgc atgaagctga cctgtaacaa gcccggatat
     6181 tgatggattg ttgctctcca ggctgtctgg gcttcaataa gtctcaacgt attggaaaac
     6241 gtcatatccc ggacgtgaag gttgactggt aacggcaggc ggacaccatt gtagttaaac
     6301 tgcatccccc tgactctggc cagttcgttc agtaattcaa ttgcatcacc ttcccagtca
     6361 aaggaaaggc gatcgctgtt agctgtcaga agatgagacc ccgttgaaac cggaagttgg
     6421 ccggaaaaca cacctttgtc ccataatgtg tactgagtgt gccgggtaac cgccacatca
     6481 gaggcagcac cgtagttacg cggaacagga gtatgctgag aaggggagtt gcagccagcc
     6541 agaaaaaggg cggcaagaca ccccgcaggt ataatacggt tcataataat tcctgagcag
     6601 ttaaatcaga atgggatata ccggtgctcc ggtacagaca ggatgatgtt tgtttccggc
     6661 tatacttgcg ttaccggaaa taaatgccag tgtggccagg taaatagcct catagtttac
     6721 tgaaggtgat tattcctgat actggctggt tgatgtgtcg tcagacataa aacgaatatg
     6781 agcgtaatct ttaacgctcc gcgccgaatc gatccatgat ggatgactcg acgatttatg
     6841 aatcgaacgt accggcgctt gtccctgaaa cagttcccgg ccctcgtccg gataacctgc
     6901 aattcagaca ggcacatgtg cccgtacact cttgtatctt actgagtata tcagaattta
     6961 tgtaaattca aatatcctct atgcctcttt gttgtatttt ccgtcaagta tgagccgggt
     7021 accatcgtaa tgcatgatca gggcatcaag gtacgccggt ttgaatttct tgccgagcct
     7081 atctctggcc agaaatgata aacaatgatt atgtgtcatt attactatgt tccttttatg
     7141 agattctctt gcgattgatt caagcgtctt ataaagatca ttattgcagt ctgataaact
     7201 gtccataacg acgggttctt taccggagaa gaattttgca gtttgtatgg ttcttaccgc
     7261 attgctggag taaatatcat attcactgaa gattgttgcg aacttaatac cttcctgctg
     7321 tgctttctct gtaccggtaa tagtgattcc gcttttatcc gaatagcatg gcatatctga
     7381 acggtcgcaa cgttctccat ggcggaaaag gaaaataacg gtgttatcag cattaatttc
     7441 ttttgcctgt tctagggtga tatcgggaag cgagcttttt gcttttgtca ctgccgctac
     7501 caggaaaaca gaaaataaaa gaatgaatgc tgtttttgaa taagaagagc gtggtttcat
     7561 catgttattt aaactgaata taaaaatacc cccgggttta gtgccggggc ggggacagta
     7621 atcagaaagt gaactgaagt tgtgccattg caccgtaatc gtggtcagtt cctaccaggc
     7681 cggaaacatc gatatgtacg acgtcaaatg gagagatacc aacgccagcg gtgaaggcgc
     7741 ttccggagtt tgatgccatg ttctgacgat aaccggcacg caattgcgcc catttccatg
     7801 cgttaaattc agcaccaaca cttgcaaact gacgtttgct gtcagaagtg aagccactgg
     7861 caggagtcag atcaacgtca agagcagtgg taaacaggtc attatgccag gatacaccgg
     7921 cggttgcctg tggacgaact ttgaaggttt ctttaaagcc attgacgact ttagtatcaa
     7981 tactgcgtgg aatgatgttc tgggcaacaa gaccgacagt ccagttgtca ttcaggtctg
     8041 tataagcacc aatatcggcg ttgaatccgt ttttcgtgtt gtggtacttg tcaccgtcaa
     8101 aatcgtcttt gtcgtaatca cgaaccgtta cgttgtagtt aaacaggtcc acacgctgat
     8161 attttggtgt gacgccaaga gaccattttt gtccggccgt ttccagttct ttggcaaatg
     8221 aaattccgac gtcggtaatt acagctgcac gcccgaatgc gcgtgaggtc agggcattct
     8281 tatcgacgtc ggtaattgtt ccattggcaa ctttgtccag ataatccagg tctgcatcgt
     8341 ttacttttcc gtttacgctg acagttccgt aagacttgac cataagggca aacggcagag
     8401 tgtcatttgc catggctgct acagctgata cacctacctg agcatctgca ttgatattgc
     8461 ggaattcctg cagacgatgt ttaagatttg ccgctgcctg ttgtacgcca tgctggttat
     8521 ctacagcact gtcaaacaaa tcccagtcat ctttaacatc atcagcttta tttgatacat
     8581 cattaggatc agcaacttgt gccccaacag acggcagaag aaggctgaaa tcatcattgc
     8641 tgttgtgctt tgtcagtaaa gccgggtttg ccagaggtgc cacaccataa tgggaagatg
     8701 ctaccccggt gccacccatt gcatcgttac gcgcttcaaa atatgacgtc gctgctgcag
     8761 cgttaaagga aaataataca gcagatgcaa tgatggtgcg ggtaatatga tttttcttca
     8821 tcatttaatt tcctgtttat cagaatttag acaaaacaac gctatgacac ggatgtgccg
     8881 taatttttta ctttggtact atctggtatt taccagggta ataaagacgt attaaataag
     8941 ctaacaggta gtatttgccc tgtaataagg ttctcggcaa ggccaggtct caacgtgacg
     9001 cgtcgcaaag acattgttgt attctgcctg gttgtatatt cagttaacca ttttcttttt
     9061 tcatcgttca gaagtgttgg tgataatgat agtgccggat atattacaga gccatttatt
     9121 tctttttcct gtctcaatat taccccgcaa tattctcttt tcgtgtttcc tgcgaagggg
     9181 aaactatgcc agaaaataac ttcagtgttg ctgctatcat taataagcat tctgaaatta
     9241 tcagcagtat ctggccattc accggacaac gctatcggat agttttcatg gccattcagc
     9301 cagtccggat tgagattaaa acattcagca agatgttcga gagcgtcagc tgaaagatag
     9361 tctgcaagtc tggccgggtc ctgtataacc gtggagcgga tattccatgg cgacagcaat
     9421 gttgccagta atggcgcatt tatatcatgc gacagcatga tgtattcaat tctgcttatt
     9481 atgtttcctg tggtgttatc cgcaggcata aacatattat gaagagcatc ctcaagcagg
     9541 attcgggtta aatcagcggt ggatatggct aattgttcgc tcattatccc cagaacttct
     9601 cttacccctt gtgggtaacg gacctgcaga ggtgtcgggt ttatctgacg accgttgatg
     9661 agtttttcgc cgattgcttt tttgtccagc gagagcatca ttcgggccgc aataccggtc
     9721 gtattcagtc tcattgttcc tccgactgag aaagtctttt cagctcatgt ggttattgta
     9781 tcttcggtgt tttttctggt gaaacgaata atttcgatca tgcagatcga atgtgtttgg
     9841 cgagttgttg caactcgcct ttctgtatgt gtcttgaatg atgtttacag cggggtgctg
     9901 cccgggttca gagggcaaat cataatgtag tcagggtcgt taacaggtaa cgctccggga
     9961 gatgggacac gttcgccttt ctgtgctgca atggccagtg tacgcaacaa aacctcactg
    10021 gcgcggtgaa ctgcttcctc tttcgttgtg gcagtgacgt gaaggttatc aaatcaccga
    10081 tctcaatacg atgcgctttc tgcccatttt cttcatcgat ggtcgtgata tgggcaggat
    10141 acggcacaaa ccatgctgcc actttttgtg tctgccgccg gcgggcatca agcttactga
    10201 ccagtagcca tgcacgaaga tgagtatacc gtttcagcat attcatggaa cgatgtcctg
    10261 atattgcagc aatctccatt acattcaggc tacccagttc gaagaagcgg cttattgctt
    10321 catgccgtag atcgtgaaaa tgcaggtcct cgatgcgaag tcgttgtgtg gctattctcc
    10381 aggcattttt aaagccggat gcggtgtaat caaaaacatt gccgtggaga ttaacgggca
    10441 tcatctgaag aaagttacgg gcacgtctgg acagaggaac atcccgtgag tgaccgtttt
    10501 ggtttcaggt aaatgagcca caccgtggcg caaatcaatg tgttcccaac gtaaggccag
    10561 tatttcgccc tgccgcatgg ctgtttcaag ggcaagatgg aaaatgacat acaacatcag
    10621 atttttttcg cggaaatagc gagaaaggcg acgttcttct gaagacgtta gccggcgatc
    10681 tcgtcccgag gatactttcg gcttgcgaac cagttcaacc gggttagtac gacaggttcc
    10741 ccattcaaca cgagcaatat tgaacagaga tgacagaagg gcgagttcaa gacgtacagt
    10801 attacctgta atgggtttac ccgttcgggg gttgatttct gctaaacgaa cgtctctgta
    10861 tgtagcaata tcaacggttg ttatttcgtc catattccga agggcaatgg gatatcgctt
    10921 gataacattg ctccggtaaa actcctgttg atgccctttc ttgtgaacag aaactgtttt
    10981 caggtactta tccagtgcgc gtgacaggga cattttacgg atgcgtggag acggcatgct
    11041 aacctcaaaa gaggcaaaaa ataatgccaa caatcattct ctgcaagcgt gccaatccgg
    11101 tacgtggaag acttctggtt cgctcaatgg ttcttacaca aacttaggtt cacatagagg
    11161 ttcattctca gggcggaatt cagggggcag tacattgttt atttatgcat ctggaggtaa
    11221 tggaggatct gccggaggtg catgtgcaaa tacatcccga ctgcagggat atgttggtgg
    11281 gacgctaatt agcgtgaatg ccagtaataa ccctgcatat ggaaaaacag cctttatcag
    11341 ctttgctgta cctgcaggta cttcctatca gataacatcc tatccaacag aaaatacatc
    11401 atgtggtgcc ggggtatttt cagtatttgg atatcaaact taattgagcg ttacacacga
    11461 cgcaatacaa cctgtcttat gctcgtattt gtaccatttg cgtgaacctg ctgggctctc
    11521 aactacctgg cagtagtggg catcctctgc gttgcccatt ctggcaatgg cacagaagtc
    11581 gaattgcccc agataacctg tggtggaaag ctgagtaact ttgagttttc cacctattgt
    11641 cccccacgta ccggattggc acgtaggcca gtgccaatcc gggacgtgga aatcatcatc
    11701 agcgtcgata tggacgaata taaaaacatt tacgttatat cccaaaaaca cacaggtctt
    11761 agggaggttt aaactgtgta ttaacaccta tcgtattgac gggcgtgaaa tggcagaaac
    11821 agaagtcgtt cctattgaca tgcctgactc taacggcgag atgacttggc aagccaaaaa
    11881 ctacacacag tattcgtcct actttatgaa gattacttgc ttgaaatagc ttattggcaa
    11941 atggcatata cagttattga accattctga tcttcggttg tatcgcacgc agccgaccat
    12001 gagttattgg ctgatgggaa tgacctggta agccatatgt aaccagttcg tgaggtgcat
    12061 attccgccgc cacctgtaac acgttttcca gttggacagg tagctgttgc tccaggaaag
    12121 cgccaacctg tggcctgtgc tgtagcaatt tgaagctcac cagatcctac ctttctccag
    12181 gtaccggatt ggcacgtagg ccagtgccaa tccggtactt ggcgtaaaag caattctggt
    12241 agtacggtga taactggtcg tatagcaaat ggtcagcaaa taccgctgcc aacagggttt
    12301 tctgccagtc agtgctcatg gagtgtgagc aatgcagaaa accctcaagg ttggaagccg
    12361 aattactttg caggatcagt tgcaacttat gatgcaaatc gaattgttaa atgtggtttt
    12421 tatgatgaat acaatttcca taaagggaca tttagagctg atttaaccgg aaaatgtagt
    12481 tatgtcgtcg cgtgtcagaa ctgagattaa attgctggcg ctgctggcct acgtgccaat
    12541 ccggtcggtg gtcagggggt aataaaatta attactcagc atgtaattgg tacaaatcat
    12601 ccgtagcaat gaatcacttt atcggcggta agagtggtgg ttcgatctat tacaagccga
    12661 ttcaatgtcc gactggatac attatgactg gaacacgcat gtacggtatt ggtgatggtg
    12721 ttgatgagga acatgtcgat gcctactgtt gcccctttaa ttaagctcca gcaccaggaa
    12781 gattgaggca tgtgactgat atggtcccac cagactcata atttccccaa gcataattac
    12841 tcacatacca aacgttgttt tggtcggaat atacctgttg aaaaccacca aagggtgagc
    12901 cattcaggta agtccatgag caatatgcat ggaccccgag ccttaaatta cgagtgtttt
    12961 tagctatgtt gtaggtctgc gttgtgaatt gaattttagg agcgccccag gtaccggatt
    13021 ggcacgcttg cagagaatga ttgttggcat tattttttgc ctcttttgag gttagcatgc
    13081 cgtctccacg catccgtaaa atgtccctgt cacgcgcact ggataagtac ctgaaaacag
    13141 tttctgttca caagaaaggg catcaacagg agttttaccg gagcaatgtt atcaagcgat
    13201 atcccattgc ccttcggaat atggacgaaa taacaaccgt tgatattgct acatacagag
    13261 acgttcgttt agcagaaatc aacccccgaa cgggtaaacc cattacaggt aatactgtac
    13321 gtcttgaact cgcccttctg tcatctctgt tcaatattgc tcgtgttgaa tggggaacct
    13381 gtcgtactaa cccggttgaa ctggttcgca agccgaaagt atcctcggga cgagatcgcc
    13441 ggctaacgtc ttcagaagaa cgtcgccttt ctcgctattt ccgcgaaaaa aatctgatgt
    13501 tgtatgtcat tttccatctt gcccttgaaa cagccatgcg gcagggcgaa atactggcct
    13561 tacgttggga acacattgat ttgcgccacg gtgtggctca tttacctgaa accaaaaacg
    13621 gtcactcacg ggatgttcct ctgtccagac gtgcccgtaa ctttcttcag atgatgcccg
    13681 ttaatctcca cggcaatgtt tttgattaca ccgcatccgg ctttaaaaat gcctggagaa
    13741 tagccacaca acgacttcgc atcgaggacc tgcattttca cgatctacgg catgaagcaa
    13801 taagccgctt cttcgaactg ggtagcctga atg
//
