LOCUS       NZ_CP023427            10105 bp    DNA     linear   CON 17-FEB-2024
DEFINITION  Enterococcus faecium strain K60-39 plasmid pTT39_p4.
ACCESSION   NZ_CP023427
VERSION     NZ_CP023427.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07638053
            Assembly: GCF_002334625.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Pedersen,T., Janice,J., Wagner,T. and Hegstad,K.
  TITLE     Enterococcus faecium megaplasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10105)
  AUTHORS   Pedersen,T., Janice,J., Wagner,T. and Hegstad,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2017) Department of Microbiology and Infection
            Control, Norwegian National Advisory Unit on Detection of
            Antimicrobial Resistance, Sykehusveien 38, Tromso, Troms 9038,
            Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method                   :: HGAP v. V3
            Assembly Name                     :: K60-39
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 256.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 10:34:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,035
            CDSs (total)                      :: 2,943
            Genes (coding)                    :: 2,774
            CDSs (with protein)               :: 2,774
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 92 of 169
            Pseudo Genes (incomplete)         :: 69 of 169
            Pseudo Genes (internal stop)      :: 28 of 169
            Pseudo Genes (multiple problems)  :: 16 of 169
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023427.1.
            Bacteria and source DNA available from University Hospital
            Nord-Norge HF Postbox 100 9038 Tromso.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="K60-39"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pTT39_p4"
                     /geo_loc_name="Norway"
                     /lat_lon="69.6492 N 18.9553 E"
                     /collection_date="2008"
                     /collected_by="Norwegian Monitoring System for Antibiotic
                     Resistance in Microbes"
     gene            <1..738
                     /locus_tag="CNX66_RS15765"
                     /old_locus_tag="CNX66_15765"
     CDS             <1..738
                     /locus_tag="CNX66_RS15765"
                     /old_locus_tag="CNX66_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_232519567.1"
                     /translation="LSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            766..1305
                     /locus_tag="CNX66_RS15770"
                     /old_locus_tag="CNX66_15770"
     CDS             766..1305
                     /locus_tag="CNX66_RS15770"
                     /old_locus_tag="CNX66_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            1321..1491
                     /locus_tag="CNX66_RS16025"
     CDS             1321..1491
                     /locus_tag="CNX66_RS16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(2087..2263)
                     /locus_tag="CNX66_RS16205"
     CDS             complement(2087..2263)
                     /locus_tag="CNX66_RS16205"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_232519568.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKFVNFIVLT"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2396..2707
                     /locus_tag="CNX66_RS15780"
                     /old_locus_tag="CNX66_15780"
     CDS             2396..2707
                     /locus_tag="CNX66_RS15780"
                     /old_locus_tag="CNX66_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_232519569.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVIYYY"
                     /name="lactococcin 972 family bacteriocin"
     gene            complement(2687..3052)
                     /locus_tag="CNX66_RS15785"
                     /old_locus_tag="CNX66_15785"
     CDS             complement(2687..3052)
                     /locus_tag="CNX66_RS15785"
                     /old_locus_tag="CNX66_15785"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_232519570.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDN"
                     /name="helix-turn-helix domain-containing protein"
     gene            3185..3559
                     /locus_tag="CNX66_RS15790"
                     /old_locus_tag="CNX66_15790"
     CDS             3185..3559
                     /locus_tag="CNX66_RS15790"
                     /old_locus_tag="CNX66_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            3613..5739
                     /locus_tag="CNX66_RS15795"
                     /old_locus_tag="CNX66_15795"
     CDS             3613..5739
                     /locus_tag="CNX66_RS15795"
                     /old_locus_tag="CNX66_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            5736..6356
                     /locus_tag="CNX66_RS15800"
                     /old_locus_tag="CNX66_15800"
     CDS             5736..6356
                     /locus_tag="CNX66_RS15800"
                     /old_locus_tag="CNX66_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            6470..6607
                     /locus_tag="CNX66_RS16030"
     CDS             6470..6607
                     /locus_tag="CNX66_RS16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            7049..7429
                     /gene="mobC"
                     /locus_tag="CNX66_RS15805"
                     /old_locus_tag="CNX66_15805"
     CDS             7049..7429
                     /gene="mobC"
                     /locus_tag="CNX66_RS15805"
                     /old_locus_tag="CNX66_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            7411..8325
                     /locus_tag="CNX66_RS15810"
                     /old_locus_tag="CNX66_15810"
     CDS             7411..8325
                     /locus_tag="CNX66_RS15810"
                     /old_locus_tag="CNX66_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002340563.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8285..8563
                     /locus_tag="CNX66_RS16350"
                     /old_locus_tag="CNX66_15815"
     CDS             8285..8563
                     /locus_tag="CNX66_RS16350"
                     /old_locus_tag="CNX66_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            8565..9254
                     /locus_tag="CNX66_RS15820"
                     /old_locus_tag="CNX66_15820"
     CDS             8565..9254
                     /locus_tag="CNX66_RS15820"
                     /old_locus_tag="CNX66_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(9375..9461)
                     /locus_tag="CNX66_RS15885"
     CDS             complement(9375..9461)
                     /locus_tag="CNX66_RS15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     BGC             1..8559
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..738
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7412..8322
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgagtaacg atatagttcg atataataat ggattaaatt ctgtcccttt aagaaaattt
       61 acccctatag atatggattt attttgggga atatgttcta aaatgaaaag aaaaggtaca
      121 aaagaagaaa tttttacatt taaacaaata aaagaattaa ttgattacaa tataaatgca
      181 cgtggcgacc gttttgctaa tgatttaata aaaatgtcaa aaaaaatcat tactttgtct
      241 tttatttatg aagacgaaaa aacatatgaa caattaaatc tgtttcaaaa atttaaaatc
      301 gataaagtga atgaaacatt attaataaag gtttctgaag aatttgaata tatacttaat
      361 tcaatcggaa ctaattttac tcgtttcgaa ttagaaaata tgacaaactt aaagagtagc
      421 tacgttaaag agttatatcg ccaacttatg gctcataaag acagaagatc taaaaaaggt
      481 cactggtttg taaaaataga agattttaga gctctattag caataccaga aagttatcgc
      541 atgagagata ttgataaaca aatttttgcc caagcgaaaa aagaattttt aaacgttaat
      601 tcaactcacc ctgctatttt taaaactttt gaaatcaaaa aaattaaagc ctacaaagga
      661 aataagatag gatctctgca aatttatttt gaagaaaata taccttcaat taccttagat
      721 aattggttag agaaataata cctaacaatt taggaggcaa ttatcatgca acccaaagaa
      781 acctttacaa taaaggaatt atctgaatta tttgaaataa gtaggcaagc aatctctaaa
      841 catgttgata aattagaccc gtccttatta gctaaaaatg agcgtggtta taaggttgta
      901 ttacgttctg gagtattgca acttgctagc aacttaggta accaaaggtt actatatagg
      961 ttgcatgagt atgaacaaga aaacgataaa aacgtttata tatcaagcaa aaacgtgatt
     1021 gtaaacgagt tgctagataa gttgcgtgaa gaaaatgaat ttttacgaga acaactttcc
     1081 ctcaaagagc aaaataattc taatatgcaa aaattactcg accagcaaca acagctcacc
     1141 ctacaggcaa atcaacagat cgaaaaactg caagaacagt tacaattagc ttatacggaa
     1201 gaaactccag aagatgaatc aactactatt ctttctgaag aaacagaatc gatagaaaaa
     1261 cacacaaaaa agaagaagca aaaatggtgg caactttgga aataactgga gggaacattc
     1321 atgacaagaa aaaatcttct attcggttct attttactag gaattctagg cttcagtccg
     1381 atgttattgt cagtaatcaa taaagggaat aaacatggtg aattgaaatt gaatccaaat
     1441 tattacaaca gtcctaacga agaattgttt gtaaaagaga cggataaata ataaaaaaga
     1501 ctgctcttcc gagcagccag ttaaaaaata atgagtgttt attcttgaaa aagcacgcgc
     1561 ttatgaagcg ctgccaaaaa tttaccatat tttacaaaaa ataacaagtc ttaaaaaaat
     1621 gacagataaa aatgcacaaa gaattagata tagataaccg cgaaaaatca agctttagaa
     1681 agttgctttt agctagacca attaaaaatc acaaagtgag cagctatagc ccttgataga
     1741 ggggctactt ggcatagtcg acattatgtc aactatgcaa ttaaataact aactacttca
     1801 tattagctta ataaaataat caaaataact agggggaaaa acattgtata aaaaaatttt
     1861 tcatttttta aataagaata gtaaatataa aaataattta agatatctat tactattaat
     1921 ccagatagag caaaccaagt aaatattcca tgtgtaaagc taaaaaaatt catttgaatc
     1981 aattcaaata aacctaatat agtaacaaat atattaaaaa taatagctgt caataccagt
     2041 aaattatttt ttttattttt tgaattatct tcatttaaaa tagaatttat gttagtacta
     2101 taaaattcac aaattttttt agtgtctcta tatctggtgt tgttttgtta ttttcccact
     2161 ttgaaatagt ttgacgtgta acgttaaatt ttcttgccac atattcttga gtataccctt
     2221 ttttcactct taattctctt agcttagatc ctaaatcctc cattttttct cctatgtaaa
     2281 cgattattta cataaaattt tatcataaaa atactaacgc gccaaaaaag ttctttattt
     2341 tataaaacac actaaaataa aataattcta gtaaaaaaga taggagaaat aagttatgaa
     2401 aaaattttta tgtctttctg ctttaatggg tgtcttatta actagtggag gaatagctag
     2461 tgccacagaa gctttaaatc tagatgtacc cgaagaccat accgccattt acggaggagg
     2521 aatggaagct acagaagaag gattttcttc tagtaaattg agatatgctg ctggcggcgg
     2581 ggatttcaac tgtggggtta atggatttaa agtttatgca aattactacc atgctagagc
     2641 taaacatagt gcaacagcta aaaatggacg aggcggtcaa gtacgatcag ttatctatta
     2701 ctattaatcc agatagagca aaccaagtaa atattccatg tgtaaagcta aaaaaattca
     2761 tttgaatcaa ttcaaataaa cctaatatag taacaaatat attaaaaata atagctgtca
     2821 ataccagtaa attatttttt ttattttttg aattatcttc atttaaaata gaatttatgt
     2881 tagtactata aaattcacaa atttttttta gtgtctctat atctggtgtt gttttgttat
     2941 tttcccactt tgaaatagtt tgacgtgtaa cgttaaattt tcttgccaca tattcttgag
     3001 tatacccttt tttcactctt aattctctta gcttagatcc taaatcctcc attttttctc
     3061 ctatgtaaac gattatttac ataaaatttt atcataaaaa tactaacgcg ccaaaaaagt
     3121 tctttatttt ataaaacaca ctaaaataaa ataattctag taaaaaagat aggagaaata
     3181 agttatgaaa aaatttttat gtctttctgc tttaatgggt gtcttattaa ctagtggagg
     3241 aatagctagt gccacagaag ctttaaatct agatgtaccc gaagaccata ccgccattta
     3301 cggaggagga atggaagcta cagaagaagg attttcttct agtaaattga gatatgctgc
     3361 tggcggcggg gatttcaact gtggggttaa tggatttaaa gtttatgcaa attactacca
     3421 tgctagagct aaacatagtg caacagctaa aaatggacga ggcggtcaag tacgatcagt
     3481 tcagaaagct ggagtaagag cttatgcaac atgcaatgcc acacttactg gaaatactgg
     3541 ttggtggaat gtttattaaa aaagagagtt taaactctct tttttattta catttgaaag
     3601 gaaattcaaa atatgttaaa aaaaataaac gctgttttat cactttttgt ctgcattaca
     3661 atactatttt tttctactaa tgttataaat ggtataaaga cttctgaagc gctatataaa
     3721 aataccgctg aaattcatat gcaatataaa gatgatatgg actttaacag ttatataaac
     3781 gctttgtatg aaatatctac acaagaaaat attaatattt ctcaatatag ttttactgct
     3841 gttaaccaac taactattct tgctacaaac cctaacgcta acaaacactg gaaattttct
     3901 aaaaaaaata tattagcaag aaacgattct aaagtccatt atactaataa caaaaattct
     3961 aagtaccaat taaaacttcc aaataatttt ttaaatataa atatttatcc tttttctcat
     4021 gtggaaaata ttggattaag tgaaatatta tatgtacaag gaaactcaaa caaattaatt
     4081 cctgtactga aaaaatatgg aacacctact atttccaata tcaatcaatc agatactttt
     4141 caaattaata ctaatattgt tcttgttatc tgctatctta gtatatttat tgtaattact
     4201 acaattatat ttgctttttc aaaaatgaaa gaaatcactc taaaaaaaat gttaggctat
     4261 aactcttttg atactataaa agattctatt aaagagttaa acctcttatt ttgttctttg
     4321 attataactt tcattttatc tatttttttg agcatatatt acagtaacga aaattttttc
     4381 ctatcatttc ttctatgtag tatttttcat tttataatta gttttttatt tatcatagtt
     4441 actctaagta tctatcattt atctagtata aaaaattatc taaaaaattc acgaccactt
     4501 aagttaaatc tttgtatatt aaatatctgt ctattcttaa gcatgatcct tctgttaata
     4561 gcttctacaa aagtaataaa tacccataat gaagccgaaa ataattctct taaatattgg
     4621 gaaagaacta ctaatttata caaaacaaat attactaatc aattaaatag aaacaacacc
     4681 gtagaagaaa ataactactt gaaaaaagct agtaaatttg tttataaaat acagaagaat
     4741 tataaaactt ttattatcgc gccatacaat tacgcaacta tccaagagaa taataaagaa
     4801 ttttttattg gacaaaaagt ttatccaaat tttgaagact atgttagtca acctgctggt
     4861 gcaggaatat gtgttgattt aaactatcta aaatattaca atcctatttc ttttgaagaa
     4921 tctactgact taaatttaat aaactctgat ccactaacta cttacattct agtacctaaa
     4981 aaatataaag aatatgcaaa aatgattgaa aaaaattatt tagaagacat acagtttaga
     5041 cttgaggact ctcctcctca agctccttat aaaaatccta atttgaaaaa tttaaaaata
     5101 aaactaatat ttgttaataa taaccaaaag tattttagtt ttaatacctt atatggaaag
     5161 gcaaaagata actatacaat aactgatccc atagttagag ttattaatcc agaaataaca
     5221 gaatcattat tttggggaaa tatattgact agtgatggtg gattattttt tgatcagaaa
     5281 catacttcaa accaaaactt ttttaatcaa attaatccgt atataaaaga atttaaccta
     5341 gaaaatatca taaatttttc ttattctgtt tttcaagaaa ctggtgaatt attatctcaa
     5401 aaaaaagtaa ataaattaaa tgcatatcta caatatcttt taatattagg attatttata
     5461 ataattaatt ttcaaatgat ttttgttagt tttactataa attcaaaaaa aatagcttta
     5521 aaaaaaatat ttggatattc accaataagt caagttttag atttatgttt actccctttt
     5581 acaatagagt tatttgctac tatgtgtttt caattgatat tttcaaaaaa cacttacttg
     5641 tattttataa tttttatgat ctttttaata aatctaatta cttctattat tatttataaa
     5701 aaaaatagtt ttaaatattt taaggagaat attttatgat attatcagtt gagaatttca
     5761 ccctgtcaat taataaaaaa aagatcaaac aaaattggaa cttttcaata tcagaaaaag
     5821 attttattat agtaacagga aaaagtggta gtggaaaaac tactctgtta aataatttaa
     5881 gcctaataga taaacaatat accggtaact tattctattt tgaaaagaaa gtttctaaac
     5941 atgatataca acgtctacga agaaatcata ttagctattt atttcaaaac tatgctcttc
     6001 ttgaaaatca aacagtacaa tacaatttcg aattagcaaa aaagtttaac agtaatattt
     6061 ccaatgatca tatttataat ttattaacag atttttcttt gccaaaagat atcttaaaag
     6121 aaaaaatctt tctcttaagc ggtggagaac aacaaagaat agcattaatt agaaatatgt
     6181 taaaaccatt tgatatatta tttgcagatg aacctacagg aaatttagat aaagaaaact
     6241 caacttttat aatgaacttt ctgaaaaatt taacagaaaa agaaaaaaaa gccgttgtac
     6301 tagtaacaca tgacttaaat gttctaaact atggaactaa tattatagaa ttataatttt
     6361 tacccgtgat gctagctgat ttaaaactag caccacgggt atttatttta tattccatct
     6421 ctcctatccc aaaatttaaa taatcactta ataaaaagga ggcacccaag tgattttatt
     6481 gaaaaaagag tatgaaaaat tttatgatag acttatttat tctttgcaat tgccaaaaaa
     6541 attaaaccgt caattacaaa agacttatga aactaaatca actgaagaat gggagagtta
     6601 ttgctagatt tttggtagaa gtaacaaaaa gtggacatat tatcataatg actcgccgaa
     6661 agtctgataa aaaattggct aaaaatattg ctgaatgttt aataaaacta tcagttttag
     6721 ctaaatagtt agtagtttac acaaaaaagc atttatgttt tttgttgcaa agtaacaatt
     6781 tcatcttcgg tgaaataagc gaaactttac aaaaagtggc tagcatttgc tagctttttt
     6841 tgtattgtgg agcgtggctt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     6901 gcggttattt gcactaaata cgtaggtatt tggctagcaa atatagagct tgccaaaccc
     6961 ttttagtgag ctttactcat tgtacctttt taggtacaat ggtattgcga acgatacgtc
     7021 cgtaggacgt actgaaaggg tgggacttat gagcgaacaa aatcaaaatt tggctagcaa
     7081 ttcttctaaa aaatatacct atcgatccga gccaaaacaa atcagttttc gagtgagcga
     7141 atccgaattt gcaaagctga agcaatcggc tgaagctttg caaatgagtg tgccggcttt
     7201 cgtgaaagcc aaggcacaaa acgcgcgtct ggtgacaccg aaagtggcgc cagacattgc
     7261 ccaggcaatc gcgcgcgatt tagccaaagc tggagggaat atcaatcaaa ttgctaagtg
     7321 gtgcaataca caccaacatg acgttgcttc tggtgacgct caacgtttat ccgaaaacct
     7381 aaaaatcatg caaaaggagc tacaaaagat atggcaacaa ttaaggtaag ccgaaccact
     7441 tcttgtagtc gtgcaattaa ttacgcagaa ccacgagcaa ccattaaaac aggaataaac
     7501 tgtgacgtcg attatgccaa aagtgaaatg aaacaaattc gcatgctgta tggaaaagac
     7561 gaccgtgtac aggcacactt attgattcaa tcttttcgac caggagaaat taccgctgaa
     7621 aaagctaatc aactaggggc agagtatgca aaaaaaatag cccccgatca ccaaacagct
     7681 atttacacac atacagacaa agaccatatt cataatcaca ttgtcattaa ctccgttaat
     7741 ctggagactg gaaaaaaatt tcaagctcat ggtcaagcat ttctaaacaa atgttatgac
     7801 ctaaatgacg agatttgtct tgcacataac ttaccaatca ctgagcgagg aaagaaacca
     7861 gaaaaacgaa ccatgtctga aataaaatta aaagaaaaaa atgaacctgt ttggaaagac
     7921 gaaattcgga ttgcgatcga tcaaacaatg aaaaacaaaa aaaccagaac ctatgaccaa
     7981 ttttgtaatt ccctcaaaac attggggatt cattgcttca atcgcgggaa aaatttcact
     8041 tacgaattga tccacaaaaa gaaaaaagta cgatccaaca gactaggaaa ggactacgaa
     8101 aaggagacga ttttacatga gctggacaga cgagaacgaa cctataacca acagcgaacc
     8161 aacgagctac gaagagctcg agctagcatt cgcgaaactg cagaagaagt acgaagagaa
     8221 caacgaactc ttacgcctga acaaccggaa attagaccgg ctcgaagaaa ccatgaacaa
     8281 cacgttgaaa gaggaacaga ccaccaacaa gaattatcgc tctaaaacgt taaaagacat
     8341 agaaaacgaa ataaagaaac aacgtgcttt tctcgttcct tatacgcaag aaaggataca
     8401 aactacgatt gatttacaag ttaaaaaact ccatcaagag ctattagacc agcgaaataa
     8461 ggaacagaaa aaggtgctta ttcaacgatc attggatttt cttgccccct tatgttgctt
     8521 agtcctgaca ctgttcgcat tttggttagt atggggaggc taaaatggca ttttatttac
     8581 cctatctact catttttgtt agtatttcag gaagtatttg gctcatctat aaaatttttc
     8641 aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgatttttc tttcttggct
     8701 gtatattttc cttgataatc gtttcaagcg gcttattcgg cgttttagag ggaaataagc
     8761 gcgtttctag aagtatttta ttaggaaatg tcactcaaaa gtatgaatca gcgcgtgata
     8821 aaaagaaaaa agagcaagca ttagcacaaa aaatggaaga gtttaccact tgttatgagg
     8881 aaatgaacga tatttttgtg aatcaagaaa aacgtttgac ggataaaaac atggaaaaac
     8941 tcactcgtct ttatcaaaac ttgccagaaa aacagcaaaa agaggttcaa gacaactatg
     9001 agcgaaccaa aaaagacgtg caatatgtaa aagatacaaa gatagaagaa gcctgtagtg
     9061 atctatttgg agacacaaac ccttggtttg ccagtgaaga agaaaaaaaa gaaaaacaac
     9121 aatctgtcac atatgaacgc tatgaaatct tacttcaaca agcaactaac atgcaaaatc
     9181 cgacaaaaaa agaaaccgcc ttaaactatc taaaaagtgt caaggaatgg ttagatcagc
     9241 aacaacaaaa ttgaatttga tttaaaatca tgttaaactg aatgtgcaaa tgatcaaaca
     9301 ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc aggttgatgt
     9361 cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa taaagagaca
     9421 caaactccta caagaagggg gcaaatgaac agttcaaaca ttagatacac ctccttccag
     9481 tcgcaagact gtcggatagg cgacaagtta ttatatctta aaaaattaaa tttgtgtggt
     9541 gtttttaagt atcacttgtt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgc
     9601 ttctcgacta gattaaggga aattaatcaa gaattgtctt agaacggctt agacaagctt
     9661 tcagagtctc acacctcctc tttgaacaac ggttttagca aaggagccgg aggcgactgc
     9721 gcagtataat atgagagcat agcgatcaca cctttttagc gaccgtaggg agcaagtttt
     9781 ccagtttaga aaaattcgcg aacctttttg gaggaaaaag taggagtttt ttaggcggga
     9841 aattcaatgt gctcttaaga tatactgtta gatatactgt tagatatact gttaataata
     9901 actgttaggg attagcaacc ttatgggaga gtaagtattg acataggtag tgggggataa
     9961 attgtcacac tatgggggat aaattgtcac actatggggg ataaattgtc acactatggg
    10021 ggataaattg tcacactatg ggggataaat tgtgttttac atcctcttat gtctatgata
    10081 acataattta ttgaggtgat tttta
//
