LOCUS       NZ_CP022921             9294 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain ST307PT03 plasmid pJYC03B, complete
            sequence.
ACCESSION   NZ_CP022921
VERSION     NZ_CP022921.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07421005
            Assembly: GCF_003597735.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Song,J.E., Jeong,H., Lim,Y.S., Ha,E.J., Jung,I.Y., Jung,W.Y.,
            Choi,H., Jeong,S.J., Ku,N.S., Yong,D., Lee,K., Kim,J.M. and
            Choi,J.Y.
  TITLE     Whole genome sequencing for investigation of a hospital outbreak of
            Klebsiella pneumoniae carbapenemase- (KPC-) producing Klebsiella
            pneumoniae (KPN) linked with an index case of community-acquired
            KPC-producing KPN infection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Song,J.E., Jeong,H., Lim,Y.S., Ha,E.J., Jung,I.Y., Jung,W.Y.,
            Choi,H., Jeong,S.J., Ku,N.S., Yong,D., Lee,K., Kim,J.M. and
            Choi,J.Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2017) Internal Medicine, Yonsei University College
            of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Korea,
            Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-JUL-2017
            Assembly Method :: SMRT Analysis (RS_HGAP_Assembly.2) v.
            2.3.0.140936.p4.150482
            Assembly Name                     :: ST307PT03_v02
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 267.17x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:11:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,466
            CDSs (total)                      :: 5,340
            Genes (coding)                    :: 5,229
            CDSs (with protein)               :: 5,229
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 49 of 111
            Pseudo Genes (incomplete)         :: 60 of 111
            Pseudo Genes (internal stop)      :: 15 of 111
            Pseudo Genes (multiple problems)  :: 13 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022921.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ST307PT03"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pJYC03B"
                     /geo_loc_name="South Korea: Seoul"
                     /lat_lon="37.57 N 126.98 E"
                     /collection_date="2015-01-31"
                     /collected_by="Yonsei University College of Medicine"
     gene            1..531
                     /locus_tag="CJ256_RS29230"
                     /old_locus_tag="CJ256_27930"
     CDS             1..531
                     /locus_tag="CJ256_RS29230"
                     /old_locus_tag="CJ256_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(559..738)
                     /locus_tag="CJ256_RS27940"
                     /old_locus_tag="CJ256_27935"
     CDS             complement(559..738)
                     /locus_tag="CJ256_RS27940"
                     /old_locus_tag="CJ256_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            807..1184
                     /locus_tag="CJ256_RS27945"
                     /old_locus_tag="CJ256_27940"
     CDS             807..1184
                     /locus_tag="CJ256_RS27945"
                     /old_locus_tag="CJ256_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(1246..1545)
                     /locus_tag="CJ256_RS27950"
                     /old_locus_tag="CJ256_27945"
     CDS             complement(1246..1545)
                     /locus_tag="CJ256_RS27950"
                     /old_locus_tag="CJ256_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1535..1798)
                     /locus_tag="CJ256_RS27955"
                     /old_locus_tag="CJ256_27950"
     CDS             complement(1535..1798)
                     /locus_tag="CJ256_RS27955"
                     /old_locus_tag="CJ256_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(2104..2523)
                     /locus_tag="CJ256_RS27960"
                     /old_locus_tag="CJ256_27955"
     CDS             complement(2104..2523)
                     /locus_tag="CJ256_RS27960"
                     /old_locus_tag="CJ256_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            2926..4611
                     /locus_tag="CJ256_RS27970"
                     /old_locus_tag="CJ256_27965"
     CDS             2926..4611
                     /locus_tag="CJ256_RS27970"
                     /old_locus_tag="CJ256_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4621..4878
                     /locus_tag="CJ256_RS27975"
                     /old_locus_tag="CJ256_27970"
     CDS             4621..4878
                     /locus_tag="CJ256_RS27975"
                     /old_locus_tag="CJ256_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            4963..5112
                     /locus_tag="CJ256_RS27980"
                     /old_locus_tag="CJ256_27975"
     CDS             4963..5112
                     /locus_tag="CJ256_RS27980"
                     /old_locus_tag="CJ256_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            6593..8557
                     /locus_tag="CJ256_RS27990"
                     /old_locus_tag="CJ256_27985"
     CDS             6593..8557
                     /locus_tag="CJ256_RS27990"
                     /old_locus_tag="CJ256_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            8557..9288
                     /locus_tag="CJ256_RS27995"
                     /old_locus_tag="CJ256_27990"
     CDS             8557..9288
                     /locus_tag="CJ256_RS27995"
                     /old_locus_tag="CJ256_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5216..5794)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8558..9285
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 atggcgctgt acatggtatt tattgaagac gcggaggacc ggaacggcga gcgctgcatc
       61 atgccgacca gcgagcatct ggaagatatg gtggtgctcg cctggcagac cggggcgtat
      121 gttttctgtt ttgtcgaccg ggcccagcat ctggcgatga tgaaatccat cgggatgtat
      181 gacgagcggc tcaacagcga cgaaaaagcc acgtactttt tcactggtaa aaatccgcaa
      241 tgggaacgga acaaacccac aggaatgaag ctgtgtgatt acctctatcg caaggtggag
      301 cggttcgcca tcgagaacgg tgtgcaggtg gttgatgccg ggaagtgctc accggagcag
      361 attaacgcga tgattgacgt ggtgagtgag cggcacgggg aaagcgcggt gaacgagaag
      421 ctgaaagcgg agctgatgcg ggaatataac agccgctatt ttaacgtgcc ggtacctgaa
      481 ctgccggagg acaaacctgc ccgccggtgg tggccgttcg gcaaaaaata aatgccagca
      541 ccggccatgc cggtgctgtt attcctcccc gagcttatcc cgcatcacct gccagagtgt
      601 ttccgcatcc tcgtgtagcc gctcgcacat gtcggccggg gtatccatat ccagctcatt
      661 cagtttatcg agcaatagca gcgactgctc cttaataaat ttagccatat tgagggctgt
      721 tttttcctgt tttgtcatga ttctttcctt ccgggcatgg tgtgcgcctg tcaggtatac
      781 tgtctcaaaa atagcggggt gagacaatgg cgtgggtgac ggtgagacag gcgacggaac
      841 tgacgggtaa agcccgaagt tccctgtatc gggatatggc gaaaggccgc gtatcatacc
      901 gtacggaagc tgatggcggc cgggtggtgg ataccagcga gcttatccgg gtgtacggtg
      961 agttaagaca ggatgagact cacgagcggg acgagttgag actgtcgggt gagactgaaa
     1021 aaatggcctc cgacgcgttg atcgcggaga taaaagcact cagggaggag gtggccgggc
     1081 tacggcagga aatgcagtcc atgagactgc tggaacacaa accggagaca gcagagaccc
     1141 cggccggaac accgcgccgg tggtggccgt ggggaaaacg ctgacgcgct gtgcttgtcc
     1201 aaccggcgac ggccacgcaa tgaattgcgt ccccgtcacc ggttattaga gaacaatatg
     1261 gggattctgg cgctggccga ccacggccag aacccggact tttctggtct ggtcatcaac
     1321 atcatagagg acatgctgtc ccatcagact gatccggcga acgtcctcgc catagcgtga
     1381 gtccacttca tagagtcgcg ggaagtcggc cagtttgtcc gccgcagcga gaacccggtc
     1441 ggtaacgttc tgcgcatccc acagggatga attttccgcg atatagtcaa acagcgtcgt
     1501 tacatcctcg atggctccct gcttccattc cacgtcatac ggcatcgcgc agttcctgat
     1561 aacggccatg caggcgctgg cggagttgtt ccgtggtcat acccggacgc gtgtcagcac
     1621 gggcggcggt cactttggcg cgaacctgat cttcatactc tgcctgggcc agttcttcgt
     1681 tagccagtcg ctgcaacagc gcagctttgt ccgggccacc ttccggcagt atgcggggca
     1741 ggctttcagc cagttcccga agaaagttgg cgttttcgta gtgtgtagtg ttgctcattg
     1801 cggagcctcc cgggttaatg cggcaatatg aaggaagttt agcagggggc ggtctgaaag
     1861 aatatggtcg tgtcagtcag gctgatgaag cagaaagtcg gtaaaaaagg gcttacggca
     1921 tccattttac gtcaaaacat atgccatgca gttgcgtgca taggcatgca ttaggataaa
     1981 atttatcggg cgcgtttccg gcagttttcc gggggggttg ttgcctgttt tatcccgtag
     2041 ccgccggaaa cgccctgagc ccgtctgagc ggtgcgcgta atgacgcgtt atggtaaatc
     2101 cacttactgt ctgccttcat agccatcgag ataaacagct gaaatggtgt cagccagtag
     2161 ccttgatttt ttcggtgtga ggtctttgct gcggtaatcg gtacccgcaa attgtgttgt
     2221 gccttggagt gagtttaaaa attcctgact gtgaaactgc tgaatgcgtt gctgcacatc
     2281 actccgtgat tttccggtcg ctttatcctt tgcgcccata tcccagaact gccggaaaaa
     2341 aggcaccatc attcgtgttg tgtcagcttt ctgggtggaa aaattgggat caaaaccgtc
     2401 atctgaagca tatacgtaat gccgggtatg ctgttccacg ctgccctttg gagcacaggc
     2461 agtgagagtt gctgtcgctg ttatcatggc gataagacac agtttgctgc cggaacgtct
     2521 catgagcgtc tccttacctt taattctttt ttctcattga gttatatatc cgcaggctat
     2581 cttatcatca gtttctgacc gtgttcagtg accgaacaac ccgaaccaga ccccccgttt
     2641 cttttcctgt cccggagggg gctttctggt atctcatata atgatttcag taaaacatac
     2701 tgaggccgga gaacggcgcc gtttgtgctg gtttttgttg catctggagg ggattgcgca
     2761 tggtgtgagt ggctgttttt ttgatcattt tcacaaaaac gcaaatttac agacttgaca
     2821 tgaaaaacaa ggcagcgtaa tttttatact gtatataaaa ccagtggtta tatgtacagt
     2881 attttatttt agtttattgt tttaaaagtc aaagaggaaa agtatatgag tggcggagac
     2941 ggacgaggtc cgggtaattc aggtctggga cacaatggtg gtcaggccag tgggaatgtg
     3001 aacggtacat caggtaaagg tggcccttca tcaggtggtg gtacggatcc aaacagcggg
     3061 ccgggctggg gtacgacgca tacacctaac ggggatattc ataactataa cccgggggag
     3121 tttggtaacg gcgggagtaa acccggtggt aatggcggta acagcggcaa tcatagcggg
     3181 agctctggtg gcggacagtc ttcggccacc gcgatggcct tcggtctgcc tgccctggcc
     3241 actccggggg ctgaaggact ggctttatcc gtttccggcg atgcgttgtc ggccgctgtt
     3301 gctgatgtgc tggctgccct gaaaggtccg tttaagtttg gtctgtgggg gattgcgatt
     3361 tacggagtgc tgccttctga gatagcaaaa gatgatccga aaatgatgtc aaaaatcgtg
     3421 acgtcattgc cggcagacac ggtaacggag acgccggtca gctccctgcc gctggaccag
     3481 gcgacggtca gcgtcactaa acgtgtggcg gatattgtga aggacgagcg gcagcatatt
     3541 gcggttgtca ccggccggcc aatgagtgtt cctgtggtgg atgcgaaacc gacaaaacgt
     3601 ccgggggtat tcagtgtgtc gattccgggt ctcccgtctc tgcaggtgag cgtacctaaa
     3661 ggtgttccgg ccgcgaaagc cccgccaaaa ggcattattg ctgaaaaagg tgattcacgt
     3721 ccggctggtt ttacggccgg tggtaactcc cgtgaggccg ttattcgttt cccgaaagag
     3781 agcggacaga aaccggtcta tgtgtcggtg acggatgttc ttactccggc acaggtaaaa
     3841 cagcgtctgg aggaagaaaa gcgtcgccag caggcatggg acgctgctca cccggaagag
     3901 gggctgaaaa gagagtatga taaagcgaaa gctgagctgg atgctgaaga taaaaatatt
     3961 gcgaccttaa acagtcgcat tgcatcgaca gagaaggcga tccccggtgc aagggctgct
     4021 gttcaggaag ccgataaaaa ggtaaaagag gcagaggcaa ataaagatga ttttgtgact
     4081 tataacccgc ctcatgaata tggctccggg tggcaggatc aggttcgcta tcttgataag
     4141 gatattcaga atcagaatga gaaattaaaa gctgcccaga catctttaaa cgaaatgaat
     4201 gaatccttat ccagggataa ggctgcgctt tccggggcga tggagagccg gaaacaaaag
     4261 gagaaaaaag cgaaggatgc agaaaataaa ttaaatgagg aaaagaaaaa acctcgtaag
     4321 ggaactaaag attacggcca tgattatttt cctgatccca agactgaaga tattaagggg
     4381 ttgggagagt tgaaagaggg taaacctaaa acccctaaac aaggtggtgg tggtaagcgg
     4441 gcccgatggt atggtgataa aaagcgtaaa atttatgaat gggattccca gcacggtgag
     4501 cttgaagggt accgcgccag tgatggcgaa cacctcgggg cattcgatcc aaaaacgggt
     4561 aagcaggtta aagggccgga tccaaaacga aacattaaaa aatatcttta agaggtaaat
     4621 atggggctta aattacatat tcattggttt gataagaaaa ccgaagagtt taaaggtggt
     4681 gaatactcaa aagacttcgg tgatgatggt tctgtcattg aaagtctggg gatgccttta
     4741 aaggataata ttaataatgg ttggtttgat gttgaaaaat catgggtttc gatattacag
     4801 ccacacttta aaaatgtaat cgatattagt aaatttgatt actttgtatc ctttgtttac
     4861 cgggatggta actggtaagc aaaagttatc aaggatgatt tgaaatacag acttccggtg
     4921 ttcagggatg aataccgggg tttttaaata cagggaaggt atatgaaaaa ggtaaaggtt
     4981 gtttttcttt ttatattgat tttttcaggc tttcttctgg tcgcatgtca ggcaaactat
     5041 atccgggatg ttcagggtgg aacggtggca ccatcgtcct cctctgaact gacggggatc
     5101 gcggttcagt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5161 tttgccctgt aaacgaaaaa accacctggg gaggtggttt gatcgaaggt taagtcagtt
     5221 ggggaactgc ttaaccgtgg taactggctt tcgcagagca cagcaaccaa atctgtcctt
     5281 ccagtgtagc cggactttgg cgcacacttc aagagcaacc gcgtgtttag ctaaacaaat
     5341 cctctgcgaa ctcccagtta ccaatggctg ctgccagtgg cgttttgcgt gcttttccgg
     5401 gttggactca agtgaacagt taccggataa ggcgcagcag tcgggctgaa cggggggttc
     5461 ttgcttacag cccagcttgg agcgaacgac ctacaccgag ccgagatacc agagtgtgag
     5521 ctatgagaaa gcgccacact tcccgcaagg gagaaaggcg gaacaggtat ccgggaaacg
     5581 gcagggttgg aacaggagag cgcaagaggg agcgattcat cggaaacggt gatgatcttt
     5641 aagtcctgtc gggtttcgcc actcctgact gattcatggt tgagccaccg gctcccacag
     5701 atgcaccgaa aaagcgtctg tttatgtgaa ctcagtcagg agggcggagc ctatggaaaa
     5761 acgccacctg cgtggccttg ccgttttgcc tcacatgtta gtcccctgct tattcacgga
     5821 atctgtgggt aactttgcat gtgtccgcag cgcccgccgc agtctcacgg ccggagcgta
     5881 gcgaccgagt gagcgaggag gcgcaatttc cccggcacct acaagacgga caccggacgc
     5941 gctgatgtgg catattgtcc cctgattaag tctttatatt ccctgataat aaattaccag
     6001 gataggtgaa tcatatcggt ttttcgctga acattttacg ttaacctatc tctgttgctt
     6061 ttttctcctt acggagaaat acaggtcatc cccgcgaggg cgtggctgac ctcaggggga
     6121 tatcccctgc ttacgctggc tggacgccac cgacgcacgg tctctccccc tctttctggc
     6181 tccgtccggc tggacgccgg ccacgccaga aattcacccg gcccccgtca ggctcacgcc
     6241 tgaccaccca caaaaatcca cggtgctgga cgcacctggc gaagcagcaa agctgctcct
     6301 gattttgcgg gtggtcagcc gctcgctatg gcgaactctg acgggggccg gaacaccgga
     6361 aaagaccgca ctggggtgcc gccttttcct ctcacacgct ggggcgtgct cgttgttcct
     6421 ccggggctgc gccttttccg catggggcgg aaagaggctg gcctccgtgg tgaaagctga
     6481 acgcaatcac cacggacgtg accgccgccg ccatgccttg gggcatgtcc gcggcttgtg
     6541 cagcgggggc tgcacatcac gccggcaaac catcgtctga aggagagaga acatgtttaa
     6601 tacggattcg cttgcctgga agtggcacat tccggagctg gcccggcaga tttatccgtg
     6661 ggtcagctgg ctgtaccagg agtattacgg tctggccggg gctttcctag ttatcggcct
     6721 gtttttcggt ctggctgaac tttgggccgg acggatgcgt tttgtggggc tgagtcataa
     6781 ggagcgacgg gagtatgccg atcgctataa gcgcgataat aaacggccag cgccattttc
     6841 ggtgctggcc tcgctggatt cgcccgggcg gatggtcagg gcacgtcgac atgtctggaa
     6901 ctacgcgtac tggctgaccg gcgcggccgg tctggccctt ctgggggggc tggttgtcgc
     6961 cgtgtttatc gagagctggg gagaaaccgt cctgcgtccg gacctctacg caaacgcaga
     7021 ttattacctg ctgcagccgg ctaacctgtt cgggattgcc tggtgcgcgg gcgggctggc
     7081 tgtcgggatt gtgttggcgc ggctgctggc gttcctgctg cttgacggtc attttgcggc
     7141 tgctgacgag gcggtgaacg cgaaactgaa tcaggagagc cggcgcagca gccagcggac
     7201 aggagagatg accgacgtgc gccacctgca tttcggggag ccggtaccgg ttaatgcgct
     7261 ggccgatttt tcctcggagc aggcccggca ggagcaggcg gtgtttctgg gtaaggatga
     7321 gcagggcaag ccggtgctgg ttccgcgtga tacctggcgc aagaccaaca ttcagatcct
     7381 cggcctgccc gggagcggca agagcgtgat ggccaccaac gcgctcattc gctgcgtacg
     7441 cgatttcggg gacgcggtgg tgtacttcga ccccaaaggg gacgcatggg cgccgcacgt
     7501 cttccgggcg cactgtccgg acttcaccct gctggatctg cggccgggga aaccggcgca
     7561 gctgaatctg ttccgcgatc tcgaccagta cgcgctgaaa aacctgctga ttgccgggct
     7621 taacctgggc gagaccggcg acgtggcgga ccactaccgc atcagcgagc agaaagcggc
     7681 gaagctgatt gcggagcagt ttccgcaggg ggcaaacatt cagcaggtgc tggcggcggc
     7741 gtacgcgctg ccggaggccc tgaaaaagga cgtgaagggg ttgatcacta ggctggaaaa
     7801 cgtcgctgac ctgagcgtcc tgcagacgga cagtggcatc gatgtggcgg ggattatcaa
     7861 cggcggcggc tgcctgtacg ttatcggctc gatggacgac gaggcggtga tccgtgtgca
     7921 aaaaatgctg tttgcccgct gcgcgcaaat catcattgcg cgggacgagt tccgggagtg
     7981 gccacacgtc agcgtgatgc tcgatgaaat taaatatctg ctgtcgaagt atgtactgaa
     8041 cgcactgggg acgctgcgca gccgggactg caatctgttg ctcgcgcacc agtcgctggg
     8101 cgatttcggg cagtgcgggc aggatctgcc cgctgaattt gtgaaaacca cggtactgga
     8161 taacacgccg atccgctggt tttaccgggc ggccagcctg gagtccgcgc agtgggcggc
     8221 ggggcagacc ggggaaatca gggtggacgt ggagcgccgc cgtgccagcc gggaggcggg
     8281 gaacgtggag catatcagcg gggacagctt catccagaag gaggcgcggc cgctgtttga
     8341 cgtgaacacc ctgcaacacc tgccggacgg gttcgcggtc atgaccgggc tgggtgtggc
     8401 ccggatggcg ttcagcagtc cgctgcgggt tgagcgccgg gaaatcccgt tgcagcgttt
     8461 cccggtgctg gcgaaaaccg atcccctggc ggagttccgg cccccggccg acggcggcgg
     8521 gccgaaagcg gatgatgatt ttgcggggat ttactgatgg cgctggagct gtacaacgtc
     8581 agccacgcga aacgacaggc gcggaacgcg gagaaaaccc ggctgaccct gcgctggttg
     8641 cgggaagagc tgtgttccac ggcggagctg gttgcgcggc ggctggggat tgcggcggtg
     8701 cagccggtct accgcttcct cgacagcctg gtggcgaaag ggctgctggt ccggaccaaa
     8761 tatccggtgg acggccgtca ggtcagcgtg tgggggctga cgccgcatgg cgtggcgttc
     8821 tcgttcgacg aggacgaacc gctggcggat gttattccgt ttcagccgtc aagggtgtcg
     8881 gcagcacatc tgccgcaccg gctggccgtg caatccctgc gccttgatat ggaagccaga
     8941 ggggccaccg gctggcgcat tctgcaccgt ctgagtctta aaggcatgaa agtgccggac
     9001 gccctggcgg agctggacgg taaaacggtg gcgtttgagg tggagcgcac cgtcaagagc
     9061 cgtcgccggt atcaggaagt ggtgtccggg tatctgttca accgcaaagc taacggcatc
     9121 gatgaaatct ggtacatctg cccggaccgg gcgacgcagg tcagggtgca gcgggcaatc
     9181 ctgtcggtgg atgaaatcgt gaacccacag accggcgagg cccgcaaaac ggcagagctg
     9241 gatcgtgaac ggttgtttgc gtgctttaaa tttatgacaa cggagtgagg gatt
//
