LOCUS       NZ_CP022918             9068 bp    DNA     linear   CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain ST307PT04 plasmid pJYC04B.
ACCESSION   NZ_CP022918
VERSION     NZ_CP022918.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07421006
            Assembly: GCF_003597695.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Song,J.E., Jeong,H., Lim,Y.S., Ha,E.J., Jung,I.Y., Jung,W.Y.,
            Choi,H., Jeong,S.J., Ku,N.S., Yong,D., Lee,K., Kim,J.M. and
            Choi,J.Y.
  TITLE     Whole genome sequencing for investigation of a hospital outbreak of
            Klebsiella pneumoniae carbapenemase- (KPC-) producing Klebsiella
            pneumoniae (KPN) linked with an index case of community-acquired
            KPC-producing KPN infection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Song,J.E., Jeong,H., Lim,Y.S., Ha,E.J., Jung,I.Y., Jung,W.Y.,
            Choi,H., Jeong,S.J., Ku,N.S., Yong,D., Lee,K., Kim,J.M. and
            Choi,J.Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2017) Internal Medicine, Yonsei University College
            of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Korea,
            Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-JUL-2017
            Assembly Method :: SMRT Analysis (RS_HGAP_Assembly.2) v.
            2.3.0.140936.p4.150482
            Assembly Name                     :: ST307PT04_v02
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 339.55x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 21:33:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,467
            CDSs (total)                      :: 5,341
            Genes (coding)                    :: 5,217
            CDSs (with protein)               :: 5,217
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 60 of 124
            Pseudo Genes (incomplete)         :: 61 of 124
            Pseudo Genes (internal stop)      :: 16 of 124
            Pseudo Genes (multiple problems)  :: 13 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022918.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ST307PT04"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pJYC04B"
                     /geo_loc_name="South Korea: Seoul"
                     /lat_lon="37.57 N 126.98 E"
                     /collection_date="2015-02"
                     /collected_by="Yonsei University College of Medicine"
     gene            complement(<1..423)
                     /locus_tag="CJ257_RS27935"
                     /old_locus_tag="CJ257_27930"
     CDS             complement(<1..423)
                     /locus_tag="CJ257_RS27935"
                     /old_locus_tag="CJ257_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045363204.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKL"
                     /name="hypothetical protein"
     gene            complement(430..1161)
                     /locus_tag="CJ257_RS27940"
                     /old_locus_tag="CJ257_27935"
     CDS             complement(430..1161)
                     /locus_tag="CJ257_RS27940"
                     /old_locus_tag="CJ257_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(1161..3125)
                     /locus_tag="CJ257_RS27945"
                     /old_locus_tag="CJ257_27940"
     CDS             complement(1161..3125)
                     /locus_tag="CJ257_RS27945"
                     /old_locus_tag="CJ257_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(4606..4755)
                     /locus_tag="CJ257_RS27955"
                     /old_locus_tag="CJ257_27950"
     CDS             complement(4606..4755)
                     /locus_tag="CJ257_RS27955"
                     /old_locus_tag="CJ257_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4840..5097)
                     /locus_tag="CJ257_RS27960"
                     /old_locus_tag="CJ257_27955"
     CDS             complement(4840..5097)
                     /locus_tag="CJ257_RS27960"
                     /old_locus_tag="CJ257_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(5107..6792)
                     /locus_tag="CJ257_RS27965"
                     /old_locus_tag="CJ257_27960"
     CDS             complement(5107..6792)
                     /locus_tag="CJ257_RS27965"
                     /old_locus_tag="CJ257_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7195..7614
                     /locus_tag="CJ257_RS27975"
                     /old_locus_tag="CJ257_27970"
     CDS             7195..7614
                     /locus_tag="CJ257_RS27975"
                     /old_locus_tag="CJ257_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            7921..8184
                     /locus_tag="CJ257_RS27980"
                     /old_locus_tag="CJ257_27975"
     CDS             7921..8184
                     /locus_tag="CJ257_RS27980"
                     /old_locus_tag="CJ257_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            8174..8473
                     /locus_tag="CJ257_RS27985"
                     /old_locus_tag="CJ257_27980"
     CDS             8174..8473
                     /locus_tag="CJ257_RS27985"
                     /old_locus_tag="CJ257_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8535..8912)
                     /locus_tag="CJ257_RS27990"
                     /old_locus_tag="CJ257_27985"
     CDS             complement(8535..8912)
                     /locus_tag="CJ257_RS27990"
                     /old_locus_tag="CJ257_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            8981..>9068
                     /locus_tag="CJ257_RS27995"
                     /old_locus_tag="CJ257_27990"
     CDS             8981..>9068
                     /locus_tag="CJ257_RS27995"
                     /old_locus_tag="CJ257_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_119948552.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNEL"
                     /name="Rop family plasmid primer RNA-binding protein"
     BGC             107..9068
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3925..4503
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(434..1161)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.31e-150"
ORIGIN
        1 cagcttctcg ttcaccgcgc tttccccgtg ccgctcactc accacgtcaa tcatcgcgtt
       61 aatctgctcc ggtgagcact tcccggcatc aaccacctgc acaccgttct cgatggcgaa
      121 ccgctccacc ttgcgataga ggtaatcaca cagcttcatt cctgtgggtt tgttccgttc
      181 ccattgcgga tttttaccag tgaaaaagta cgtggctttt tcgtcgctgt tgagccgctc
      241 gtcatacatc ccgatggatt tcatcatcgc cagatgctgg gcccggtcga caaaacagaa
      301 aacatacgcc ccggtctgcc aggcgagcac caccatatct tccagatgct cgctggtcgg
      361 catgatgcag cgctcgccgt tccggtcctc cgcgtcttca ataaatacca tgtacagcgc
      421 cataatccct cactccgttg tcataaattt aaagcacgca aacaaccgtt cacgatccag
      481 ctctgccgtt ttgcgggcct cgccggtctg tgggttcacg atttcatcca ccgacaggat
      541 tgcccgctgc accctgacct gcgtcgcccg gtccgggcag atgtaccaga tttcatcgat
      601 gccgttagct ttgcggttga acagataccc ggacaccact tcctgatacc ggcgacggct
      661 cttgacggtg cgctccacct caaacgccac cgttttaccg tccagctccg ccagggcgtc
      721 cggcactttc atgcctttaa gactcagacg gtgcagaatg cgccagccgg tggcccctct
      781 ggcttccata tcaaggcgca gggattgcac ggccagccgg tgcggcagat gtgctgccga
      841 cacccttgac ggctgaaacg gaataacatc cgccagcggt tcgtcctcgt cgaacgagaa
      901 cgccacgcca tgcggcgtca gcccccacac gctgacctga cggccgtcca ccggatattt
      961 ggtccggacc agcagccctt tcgccaccag gctgtcgagg aagcggtaga ccggctgcac
     1021 cgccgcaatc cccagccgcc gcgcaaccag ctccgccgtg gaacacagct cttcccgcaa
     1081 ccagcgcagg gtcagccggg ttttctccgc gttccgcgcc tgtcgtttcg cgtggctgac
     1141 gttgtacagc tccagcgcca tcagtaaatc cccgcaaaat catcatccgc tttcggcccg
     1201 ccgccgtcgg ccgggggccg gaactccgcc aggggatcgg ttttcgccag caccgggaaa
     1261 cgctgcaacg ggatttcccg gcgctcaacc cgcagcggac tgctgaacgc catccgggcc
     1321 acacccagcc cggtcatgac cgcgaacccg tccggcaggt gttgcagggt gttcacgtca
     1381 aacagcggcc gcgcctcctt ctggatgaag ctgtccccgc tgatatgctc cacgttcccc
     1441 gcctcccggc tggcacggcg gcgctccacg tccaccctga tttccccggt ctgccccgcc
     1501 gcccactgcg cggactccag gctggccgcc cggtaaaacc agcggatcgg cgtgttatcc
     1561 agtaccgtgg ttttcacaaa ttcagcgggc agatcctgcc cgcactgccc gaaatcgccc
     1621 agcgactggt gcgcgagcaa cagattgcag tcccggctgc gcagcgtccc cagtgcgttc
     1681 agtacatact tcgacagcag atatttaatt tcatcgagca tcacgctgac gtgtggccac
     1741 tcccggaact cgtcccgcgc aatgatgatt tgcgcgcagc gggcaaacag cattttttgc
     1801 acacggatca ccgcctcgtc gtccatcgag ccgataacgt acaggcagcc gccgccgttg
     1861 ataatccccg ccacatcgat gccactgtcc gtctgcagga cgctcaggtc agcgacgttt
     1921 tccagcctag tgatcaaccc cttcacgtcc tttttcaggg cctccggcag cgcgtacgcc
     1981 gccgccagca cctgctgaat gtttgccccc tgcggaaact gctccgcaat cagcttcgcc
     2041 gctttctgct cgctgatgcg gtagtggtcc gccacgtcgc cggtctcgcc caggttaagc
     2101 ccggcaatca gcaggttttt cagcgcgtac tggtcgagat cgcggaacag attcagctgc
     2161 gccggtttcc ccggccgcag atccagcagg gtgaagtccg gacagtgcgc ccggaagacg
     2221 tgcggcgccc atgcgtcccc tttggggtcg aagtacacca ccgcgtcccc gaaatcgcgt
     2281 acgcagcgaa tgagcgcgtt ggtggccatc acgctcttgc cgctcccggg caggccgagg
     2341 atctgaatgt tggtcttgcg ccaggtatca cgcggaacca gcaccggctt gccctgctca
     2401 tccttaccca gaaacaccgc ctgctcctgc cgggcctgct ccgaggaaaa atcggccagc
     2461 gcattaaccg gtaccggctc cccgaaatgc aggtggcgca cgtcggtcat ctctcctgtc
     2521 cgctggctgc tgcgccggct ctcctgattc agtttcgcgt tcaccgcctc gtcagcagcc
     2581 gcaaaatgac cgtcaagcag caggaacgcc agcagccgcg ccaacacaat cccgacagcc
     2641 agcccgcccg cgcaccaggc aatcccgaac aggttagccg gctgcagcag gtaataatct
     2701 gcgtttgcgt agaggtccgg acgcaggacg gtttctcccc agctctcgat aaacacggcg
     2761 acaaccagcc cccccagaag ggccagaccg gccgcgccgg tcagccagta cgcgtagttc
     2821 cagacatgtc gacgtgccct gaccatccgc ccgggcgaat ccagcgaggc cagcaccgaa
     2881 aatggcgctg gccgtttatt atcgcgctta tagcgatcgg catactcccg tcgctcctta
     2941 tgactcagcc ccacaaaacg catccgtccg gcccaaagtt cagccagacc gaaaaacagg
     3001 ccgataacta ggaaagcccc ggccagaccg taatactcct ggtacagcca gctgacccac
     3061 ggataaatct gccgggccag ctccggaatg tgccacttcc aggcaagcga atccgtatta
     3121 aacatgttct ctctccttca gacgatggtt tgccggcgtg atgtgcagcc cccgctgcac
     3181 aagccgcgga catgccccaa ggcatggcgg cggcggtcac gtccgtggtg attgcgttca
     3241 gctttcacca cggaggccag cctctttccg ccccatgcgg aaaaggcgca gccccggagg
     3301 aacaacgagc acgccccagc gtgtgagagg aaaaggcggc accccagtgc ggtcttttcc
     3361 ggtgttccgg cccccgtcag agttcgccat agcgagcggc tgaccacccg caaaatcagg
     3421 agcagctttg ctgcttcgcc aggtgcgtcc agcaccgtgg atttttgtgg gtggtcaggc
     3481 gtgagcctga cgggggccgg gtgaatttct ggcgtggccg gcgtccagcc ggacggagcc
     3541 agaaagaggg ggagagaccg tgcgtcggtg gcgtccagcc agcgtaagca ggggatatcc
     3601 ccctgaggtc agccacgccc tcgcggggat gacctgtatt tctccgtaag gagaaaaaag
     3661 caacagagat aggttaacgt aaaatgttca gcgaaaaacc gatatgattc acctatcctg
     3721 gtaatttatt atcagggaat ataaagactt aatcagggga caatatgcca catcagcgcg
     3781 tccggtgtcc gtcttgtagg tgccggggaa attgcgcctc ctcgctcact cggtcgctac
     3841 gctccggccg tgagactgcg gcgggcgctg cggacacatg caaagttacc cacagattcc
     3901 gtgaataagc aggggactaa catgtgaggc aaaacggcaa ggccacgcag gtggcgtttt
     3961 tccataggct ccgccctcct gactgagttc acataaacag acgctttttc ggtgcatctg
     4021 tgggagccgg tggctcaacc atgaatcagt caggagtggc gaaacccgac aggacttaaa
     4081 gatcatcacc gtttccgatg aatcgctccc tcttgcgctc tcctgttcca accctgccgt
     4141 ttcccggata cctgttccgc ctttctccct tgcgggaagt gtggcgcttt ctcatagctc
     4201 acactctggt atctcggctc ggtgtaggtc gttcgctcca agctgggctg taagcaagaa
     4261 ccccccgttc agcccgactg ctgcgcctta tccggtaact gttcacttga gtccaacccg
     4321 gaaaagcacg caaaacgcca ctggcagcag ccattggtaa ctgggagttc gcagaggatt
     4381 tgtttagcta aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct acactggaag
     4441 gacagatttg gttgctgtgc tctgcgaaag ccagttacca cggttaagca gttccccaac
     4501 tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga
     4561 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact gaaccgcgat
     4621 ccccgtcagt tcagaggagg acgatggtgc caccgttcca ccctgaacat cccggatata
     4681 gtttgcctga catgcgacca gaagaaagcc tgaaaaaatc aatataaaaa gaaaaacaac
     4741 ctttaccttt ttcatatacc ttccctgtat ttaaaaaccc cggtattcat ccctgaacac
     4801 cggaagtctg tatttcaaat catccttgat aacttttgct taccagttac catcccggta
     4861 aacaaaggat acaaagtaat caaatttact aatatcgatt acatttttaa agtgtggctg
     4921 taatatcgaa acccatgatt tttcaacatc aaaccaacca ttattaatat tatcctttaa
     4981 aggcatcccc agactttcaa tgacagaacc atcatcaccg aagtcttttg agtattcacc
     5041 acctttaaac tcttcggttt tcttatcaaa ccaatgaata tgtaatttaa gccccatatt
     5101 tacctcttaa agatattttt taatgtttcg ttttggatcc ggccctttaa cctgcttacc
     5161 cgtttttgga tcgaatgccc cgaggtgttc gccatcactg gcgcggtacc cttcaagctc
     5221 accgtgctgg gaatcccatt cataaatttt acgcttttta tcaccatacc atcgggcccg
     5281 cttaccacca ccaccttgtt taggggtttt aggtttaccc tctttcaact ctcccaaccc
     5341 cttaatatct tcagtcttgg gatcaggaaa ataatcatgg ccgtaatctt tagttccctt
     5401 acgaggtttt ttcttttcct catttaattt attttctgca tccttcgctt ttttctcctt
     5461 ttgtttccgg ctctccatcg ccccggaaag cgcagcctta tccctggata aggattcatt
     5521 catttcgttt aaagatgtct gggcagcttt taatttctca ttctgattct gaatatcctt
     5581 atcaagatag cgaacctgat cctgccaccc ggagccatat tcatgaggcg ggttataagt
     5641 cacaaaatca tctttatttg cctctgcctc ttttaccttt ttatcggctt cctgaacagc
     5701 agcccttgca ccggggatcg ccttctctgt cgatgcaatg cgactgttta aggtcgcaat
     5761 atttttatct tcagcatcca gctcagcttt cgctttatca tactctcttt tcagcccctc
     5821 ttccgggtga gcagcgtccc atgcctgctg gcgacgcttt tcttcctcca gacgctgttt
     5881 tacctgtgcc ggagtaagaa catccgtcac cgacacatag accggtttct gtccgctctc
     5941 tttcgggaaa cgaataacgg cctcacggga gttaccaccg gccgtaaaac cagccggacg
     6001 tgaatcacct ttttcagcaa taatgccttt tggcggggct ttcgcggccg gaacaccttt
     6061 aggtacgctc acctgcagag acgggagacc cggaatcgac acactgaata cccccggacg
     6121 ttttgtcggt ttcgcatcca ccacaggaac actcattggc cggccggtga caaccgcaat
     6181 atgctgccgc tcgtccttca caatatccgc cacacgttta gtgacgctga ccgtcgcctg
     6241 gtccagcggc agggagctga ccggcgtctc cgttaccgtg tctgccggca atgacgtcac
     6301 gatttttgac atcattttcg gatcatcttt tgctatctca gaaggcagca ctccgtaaat
     6361 cgcaatcccc cacagaccaa acttaaacgg acctttcagg gcagccagca catcagcaac
     6421 agcggccgac aacgcatcgc cggaaacgga taaagccagt ccttcagccc ccggagtggc
     6481 cagggcaggc agaccgaagg ccatcgcggt ggccgaagac tgtccgccac cagagctccc
     6541 gctatgattg ccgctgttac cgccattacc accgggttta ctcccgccgt taccaaactc
     6601 ccccgggtta tagttatgaa tatccccgtt aggtgtatgc gtcgtacccc agcccggccc
     6661 gctgtttgga tccgtaccac cacctgatga agggccacct ttacctgatg taccgttcac
     6721 attcccactg gcctgaccac cattgtgtcc cagacctgaa ttacccggac ctcgtccgtc
     6781 tccgccactc atatactttt cctctttgac ttttaaaaca ataaactaaa ataaaatact
     6841 gtacatataa ccactggttt tatatacagt ataaaaatta cgctgccttg tttttcatgt
     6901 caagtctgta aatttgcgtt tttgtgaaaa tgatcaaaaa aacagccact cacaccatgc
     6961 gcaatcccct ccagatgcaa caaaaaccag cacaaacggc gccgttctcc ggcctcagta
     7021 tgttttactg aaatcattat atgagatacc agaaagcccc ctccgggaca ggaaaagaaa
     7081 cggggggtct ggttcgggtt gttcggtcac tgaacacggt cagaaactga tgataagata
     7141 gcctgcggat atataactca atgagaaaaa agaattaaag gtaaggagac gctcatgaga
     7201 cgttccggca gcaaactgtg tcttatcgcc atgataacag cgacagcaac tctcactgcc
     7261 tgtgctccaa agggcagcgt ggaacagcat acccggcatt acgtatatgc ttcagatgac
     7321 ggttttgatc ccaatttttc cacccagaaa gctgacacaa cacgaatgat ggtgcctttt
     7381 ttccggcagt tctgggatat gggcgcaaag gataaagcga ccggaaaatc acggagtgat
     7441 gtgcagcaac gcattcagca gtttcacagt caggaatttt taaactcact ccaaggcaca
     7501 acacaatttg cgggtaccga ttaccgcagc aaagacctca caccgaaaaa atcaaggcta
     7561 ctggctgaca ccatttcagc tgtttatctc gatggctatg aaggcagaca gtaagtggat
     7621 ttaccataac gcgtcattac gcgcaccgct cagacgggct cagggcgttt ccggcggcta
     7681 cgggataaaa caggcaacaa ccccccccgg aaaactgccg gaaacgcgcc cgataaattt
     7741 tatcctaatg catgcctatg cacgcaactg catggcatat gttttgacgt aaaatggatg
     7801 ccgtaagccc ttttttaccg actttctgct tcatcagcct gactgacacg accatattct
     7861 ttcagaccgc cccctgctaa acttccttca tattgccgca ttaacccggg aggctccgca
     7921 atgagcaaca ctacacacta cgaaaacgcc aactttcttc gggaactggc tgaaagcctg
     7981 ccccgcatac tgccggaagg tggcccggac aaagctgcgc tgttgcagcg actggctaac
     8041 gaagaactgg cccaggcaga gtatgaagat caggttcgcg ccaaagtgac cgccgcccgt
     8101 gctgacacgc gtccgggtat gaccacggaa caactccgcc agcgcctgca tggccgttat
     8161 caggaactgc gcgatgccgt atgacgtgga atggaagcag ggagccatcg aggatgtaac
     8221 gacgctgttt gactatatcg cggaaaattc atccctgtgg gatgcgcaga acgttaccga
     8281 ccgggttctc gctgcggcgg acaaactggc cgacttcccg cgactctatg aagtggactc
     8341 acgctatggc gaggacgttc gccggatcag tctgatggga cagcatgtcc tctatgatgt
     8401 tgatgaccag accagaaaag tccgggttct ggccgtggtc ggccagcgcc agaatcccca
     8461 tattgttctc taataaccgg tgacggggac gcaattcatt gcgtggccgt cgccggttgg
     8521 acaagcacag cgcgtcagcg ttttccccac ggccaccacc ggcgcggtgt tccggccggg
     8581 gtctctgctg tctccggttt gtgttccagc agtctcatgg actgcatttc ctgccgtagc
     8641 ccggccacct cctccctgag tgcttttatc tccgcgatca acgcgtcgga ggccattttt
     8701 tcagtctcac ccgacagtct caactcgtcc cgctcgtgag tctcatcctg tcttaactca
     8761 ccgtacaccc ggataagctc gctggtatcc accacccggc cgccatcagc ttccgtacgg
     8821 tatgatacgc ggcctttcgc catatcccga tacagggaac ttcgggcttt acccgtcagt
     8881 tccgtcgcct gtctcaccgt cacccacgcc attgtctcac cccgctattt ttgagacagt
     8941 atacctgaca ggcgcacacc atgcccggaa ggaaagaatc atgacaaaac aggaaaaaac
     9001 agccctcaat atggctaaat ttattaagga gcagtcgctg ctattgctcg ataaactgaa
     9061 tgagctgg
//
