LOCUS       NZ_CP022687            11499 bp    DNA     linear   CON 17-JUN-2024
DEFINITION  Escherichia coli Nissle 1917 plasmid pNissle1.
ACCESSION   NZ_CP022687
VERSION     NZ_CP022687.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07451663
            Assembly: GCF_003546975.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli Nissle 1917
  ORGANISM  Escherichia coli Nissle 1917
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11499)
  AUTHORS   McClelland,M. and Chu,W.
  TITLE     E. coli Nissle Genome Assembly Including Plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11499)
  AUTHORS   McClelland,M. and Chu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2017) Microbiology and Molecular Genetics,
            University of California, B240 Medical Sciences, Irvine, CA
            92697-4025, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. V2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 299.1x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003546975.1-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 06:53:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,875
            CDSs (total)                      :: 4,761
            Genes (coding)                    :: 4,537
            CDSs (with protein)               :: 4,537
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 95 of 224
            Pseudo Genes (incomplete)         :: 141 of 224
            Pseudo Genes (internal stop)      :: 53 of 224
            Pseudo Genes (multiple problems)  :: 54 of 224
            Pseudo Genes (short protein)      :: 2 of 224
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022687.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11499
                     /organism="Escherichia coli Nissle 1917"
                     /mol_type="genomic DNA"
                     /strain="Nissle 1917"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:316435"
                     /plasmid="pNissle1"
                     /geo_loc_name="Germany: Freiburg"
                     /altitude="279 m"
                     /collection_date="1917"
                     /collected_by="Nissle"
     gene            <1..592
                     /locus_tag="CIW80_RS25555"
                     /old_locus_tag="CIW80_25800"
     CDS             <1..592
                     /locus_tag="CIW80_RS25555"
                     /old_locus_tag="CIW80_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_189328174.1"
                     /translation="SAPLKYAAAIENALRKKLGADAGYSGLICKNPNHLHWQITVWQPE
                     LYTLDWLADYLDLGAANDREILPDYGLGRNCTLFDKTRKWAYRAIRQGWPQYDQWLQAC
                     IERAKAYNLQFSAPLDENEVMGIAKSVAKWTSKNLTELGFEEYVKQTHTSEIQRQRGKK
                     SSGGGRPKVRDGEWISLGISRSTWYRKFYKNEN"
                     /name="replication initiation protein"
     gene            582..1229
                     /gene="kwaA"
                     /locus_tag="CIW80_RS25560"
                     /old_locus_tag="CIW80_25805"
     CDS             582..1229
                     /gene="kwaA"
                     /locus_tag="CIW80_RS25560"
                     /old_locus_tag="CIW80_25805"
                     /inference="COORDINATES: protein motif:HMM:NF041622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage protein KwaA"
                     /protein_id="WP_024199421.1"
                     /translation="MKIRMYLLSMLFLFIPITIYFSGIDNVWSVLDKAMSSCSGADGKF
                     QCVLDYSNSRLTFSVLRSVICGALILTCSYTYIWYRWVLKNLDSGAVSRKLKVVSCKKE
                     SGEYLSFLSTYIMPLVFTDLSKPSNIVNFLFVLIIVGFLHIKTKRIHCNPTLSLFNVSA
                     YKITYSVVANGREKTQDGELIVLSKDLIKENDFIRVINHDDYLTFAKKIIEE"
                     /name="kwaA"
     gene            1232..2374
                     /locus_tag="CIW80_RS25565"
                     /old_locus_tag="CIW80_25810"
     CDS             1232..2374
                     /locus_tag="CIW80_RS25565"
                     /old_locus_tag="CIW80_25810"
                     /inference="COORDINATES: protein motif:HMM:NF027488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kiwa anti-phage protein KwaB-like
                     domain-containing protein"
                     /protein_id="WP_001704287.1"
                     /translation="MSDDALDLDALEHNEEKAQELLELYKHFDCDINDVTLHTYFNFKN
                     KGKAGAKDSFSYKIAILKNDSELKQSIIDNVIKKTISDLENRVVIVSDVNTLSDRTNTI
                     SFLNDAEQYDNEQMKFLTGIVFDAGFGGDGNENIPIVDFDSVDLISKVDSIIFVLSKNN
                     DDNCITIYKKQYPVQNLSRKKNNFYGFSKKELSRVDQDMISIDGKASLVFAGDTVFIYD
                     QNVFERFFGYKAWIKNAATDFVNNMSTRFKGFVDLSKFIERVNKDSHDADAFSRKLAKA
                     YARSETKQTLQTITNEHLKSVLDENGYLAGVLKYKKGDATICIASHNQQDTFIAMVSEG
                     VLRSLITGIDYLSLGSKRKISSAASNTTTSTTSDSSSAAV"
                     /name="Kiwa anti-phage protein KwaB-like domain-containing
                     protein"
     gene            2601..2927
                     /locus_tag="CIW80_RS25570"
                     /old_locus_tag="CIW80_25815"
     CDS             2601..2927
                     /locus_tag="CIW80_RS25570"
                     /old_locus_tag="CIW80_25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001554615.1"
                     /translation="MEKRTKEIKIRLTEAEHQRLLERCDRKHLAEWLRAVGLGESRTAR
                     RRPLPTVDPTLLRQVSGIGNNLNQIARYLNQHGLPPQERVSLLAVLNSIDQHLAELLEQ
                     HRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3010..>3603
                     /locus_tag="CIW80_RS26610"
                     /old_locus_tag="CIW80_25820"
                     /pseudo=""
     CDS             3010..>3603
                     /locus_tag="CIW80_RS26610"
                     /old_locus_tag="CIW80_25820"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4160..4384
                     /locus_tag="CIW80_RS25580"
                     /old_locus_tag="CIW80_25825"
     CDS             4160..4384
                     /locus_tag="CIW80_RS25580"
                     /old_locus_tag="CIW80_25825"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_223938297.1"
                     /translation="MEQQLLSALESLQRHYEQQQQAWQDSYANLQRMFEVTSQELAKND
                     RVCQALSMQVTGLAQQVESLNRTVRRLSN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4429..4713)
                     /locus_tag="CIW80_RS25585"
                     /old_locus_tag="CIW80_25830"
     CDS             complement(4429..4713)
                     /locus_tag="CIW80_RS25585"
                     /old_locus_tag="CIW80_25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016149473.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001554709.1"
                     /translation="MSYNLEFDPRALKEWRKLGDDVRLQFKKKLEQVLQHPRIDKNRLR
                     ELHDCYKIKLRASGYRLVYQVRDQTITVFVVAVGKRERSAAYDAADKRL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4703..4945)
                     /locus_tag="CIW80_RS25590"
                     /old_locus_tag="CIW80_25835"
     CDS             complement(4703..4945)
                     /locus_tag="CIW80_RS25590"
                     /old_locus_tag="CIW80_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020301039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_001554710.1"
                     /translation="MATINIRIDDELKSRSYAALEKLGVTPSEVLRQTLEYVAQSGRLP
                     FQQVLLTEDDADLMAIVRDRLENPQAGVKVSLDEL"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            5210..6106
                     /locus_tag="CIW80_RS25595"
                     /old_locus_tag="CIW80_25840"
     CDS             5210..6106
                     /locus_tag="CIW80_RS25595"
                     /old_locus_tag="CIW80_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_024199422.1"
                     /translation="MDNQALTLFNDRLPHKPYFSDDLQFGVRIAGKERALLAKYIQFNQ
                     PHAMYWLCFDVDRAGAAIDWADLGAPAPTLTIKNPENGHAHLLYALHTAVRTAPDGRAA
                     PLKYAAAIENALRKKLGADAGYSGLICKNPNHLHWQITVWQPELYTLDWLADYLDLGAA
                     NDREILPDYGLGRNCTLFDKTRKWAYRAIRQGWPQYDQWLQACIERAKAYNLQFSAPLD
                     ENEVMGIAKSVAKWTSKNLTELGFEEYVKQTHTSEIQRQRGKKSSGGGRPKVRDGEWIS
                     LGISRSTWYRKFYKNEN"
                     /name="replication initiation protein"
     gene            6096..6743
                     /gene="kwaA"
                     /locus_tag="CIW80_RS25600"
                     /old_locus_tag="CIW80_25845"
     CDS             6096..6743
                     /gene="kwaA"
                     /locus_tag="CIW80_RS25600"
                     /old_locus_tag="CIW80_25845"
                     /inference="COORDINATES: protein motif:HMM:NF041622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage protein KwaA"
                     /protein_id="WP_024199421.1"
                     /translation="MKIRMYLLSMLFLFIPITIYFSGIDNVWSVLDKAMSSCSGADGKF
                     QCVLDYSNSRLTFSVLRSVICGALILTCSYTYIWYRWVLKNLDSGAVSRKLKVVSCKKE
                     SGEYLSFLSTYIMPLVFTDLSKPSNIVNFLFVLIIVGFLHIKTKRIHCNPTLSLFNVSA
                     YKITYSVVANGREKTQDGELIVLSKDLIKENDFIRVINHDDYLTFAKKIIEE"
                     /name="kwaA"
     gene            7220..7540
                     /gene="mobA"
                     /locus_tag="CIW80_RS26615"
     CDS             7220..7540
                     /gene="mobA"
                     /locus_tag="CIW80_RS26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_284142498.1"
                     /translation="MSKNNDDNCITIYKKQXRLTAEEDQEIRKRAAECGKTVSGFLRAA
                     ALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRLM
                     AD"
                     /name="mobA"
     gene            complement(7929..8357)
                     /locus_tag="CIW80_RS25610"
                     /old_locus_tag="CIW80_25855"
     CDS             complement(7929..8357)
                     /locus_tag="CIW80_RS25610"
                     /old_locus_tag="CIW80_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            8515..8685
                     /locus_tag="CIW80_RS26150"
     CDS             8515..8685
                     /locus_tag="CIW80_RS26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001531327.1"
                     /translation="MKTEQRHDLVKDIEVFGVSLSLLISRANEKSVTMPSGLSREQRRA
                     WAAEQARKIHN"
                     /name="hypothetical protein"
     gene            8649..9077
                     /locus_tag="CIW80_RS25615"
                     /old_locus_tag="CIW80_25860"
     CDS             8649..9077
                     /locus_tag="CIW80_RS25615"
                     /old_locus_tag="CIW80_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            complement(9293..9424)
                     /locus_tag="CIW80_RS26155"
     CDS             complement(9293..9424)
                     /locus_tag="CIW80_RS26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023151671.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLATATGKQDVREM"
                     /name="hypothetical protein"
     gene            complement(9912..10106)
                     /locus_tag="CIW80_RS25625"
                     /old_locus_tag="CIW80_25870"
     CDS             complement(9912..10106)
                     /locus_tag="CIW80_RS25625"
                     /old_locus_tag="CIW80_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041622.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALNADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            10390..10713
                     /gene="mobA"
                     /locus_tag="CIW80_RS25630"
                     /old_locus_tag="CIW80_25875"
     CDS             10390..10713
                     /gene="mobA"
                     /locus_tag="CIW80_RS25630"
                     /old_locus_tag="CIW80_25875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(11102..>11499)
                     /locus_tag="CIW80_RS25635"
                     /old_locus_tag="CIW80_25880"
     CDS             complement(11102..>11499)
                     /locus_tag="CIW80_RS25635"
                     /old_locus_tag="CIW80_25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_038427369.1"
                     /translation="GGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNLIVHWWSLQEWP
                     HPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNIYDLTKLVDYLD
                     TMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     Typing          10145..10381
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          4..592
                     /name="rep_cluster_2486"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5073..6106
                     /name="rep_cluster_2486"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9353..9877)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.085"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          2918..4431
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.802"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7263..7537
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.652"
                     /coverage="100.0"
                     /evalue="1.12e-50"
     Typing          10391..10710
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.15e-62"
ORIGIN
        1 ctctgccccc cttaaatacg ccgccgccat cgagaatgcg ctgcgtaaaa aattgggcgc
       61 agatgcgggg tattcagggc taatttgcaa gaatccgaac cacctgcact ggcagatcac
      121 cgtctggcag ccagagctct atacccttga ctggttagcc gattacctcg accttggcgc
      181 agccaatgac cgcgaaatcc tgccggacta cggtttaggc cgtaactgca ccctcttcga
      241 taaaacccgc aagtgggctt accgcgctat ccgccaaggc tggccgcagt atgaccaatg
      301 gctacaagcc tgcattgaac gcgctaaagc ctacaacctg cagttctccg cacctttaga
      361 cgagaacgaa gtcatgggaa ttgctaaaag tgtggctaag tggacaagta agaaccttac
      421 agagcttggc tttgaagagt atgttaaaca aacacatact tctgaaattc agcgtcaacg
      481 tggaaaaaaa agctctggtg gtggaaggcc gaaagttaga gatggagaat ggatttcttt
      541 aggaataagt agatcaacgt ggtacaggaa attttacaaa aatgaaaatt agaatgtatc
      601 tcttatcaat gttgttttta tttataccga taacaatata tttttctggt attgataacg
      661 tatggtctgt tttagataag gccatgtctt cgtgttctgg cgcggatgga aaatttcaat
      721 gcgtactcga ctattcaaat agcagattaa cattttctgt attgaggtca gtgatctgcg
      781 gggctttgat tttaacttgt tcttatacat acatttggta tcgatgggtt ttgaaaaact
      841 tagactccgg tgcggtgtca agaaagttaa aggtcgtttc atgtaaaaaa gaatccggtg
      901 agtatctttc ttttttatca acgtacataa tgccgttggt ctttactgat ttaagcaaac
      961 catcaaatat agttaatttt ttgtttgttt tgattatcgt cggttttttg cacatcaaaa
     1021 caaaaagaat tcactgcaac ccaacattgt cattatttaa tgtttctgcc tataagatta
     1081 cttacagtgt ggtagctaat ggaagggaaa aaacgcaaga cggtgagttg atagttttat
     1141 ctaaagattt aataaaagaa aatgatttta tcagagttat aaatcatgat gattatttaa
     1201 cgttcgcgaa aaaaatcata gaggaatgat gatgagtgac gatgccttag atcttgatgc
     1261 tttggagcat aatgaagaaa aagcacaaga gcttttagaa ctgtataagc attttgattg
     1321 tgatattaac gatgttacat tgcacactta ctttaacttt aaaaataaag gtaaagcggg
     1381 tgctaaggac tcttttagct ataaaattgc catccttaaa aatgacagtg agctaaaaca
     1441 gtccattatt gataatgtga taaagaaaac tatatctgat ttagaaaatc gggttgtgat
     1501 agtatcagat gttaatacac taagtgaccg caccaacacc atttcatttt taaatgatgc
     1561 tgagcagtat gataatgagc aaatgaagtt tctgactggc attgtgtttg atgctgggtt
     1621 tggtggtgat ggcaatgaaa atatcccaat tgttgatttt gacagtgtgg atctaatatc
     1681 aaaagttgac tcaatcattt tcgttttgag taagaataat gacgacaatt gtataaccat
     1741 atacaagaag caatatccag ttcaaaactt atcaagaaag aaaaataatt tttatgggtt
     1801 tagcaaaaaa gaactatctc gagtagatca agatatgatt tctattgatg gtaaggcatc
     1861 tcttgttttt gctggcgata cggtatttat ctacgaccaa aatgttttcg agcgtttttt
     1921 tggctataaa gcatggataa aaaatgctgc cactgatttt gttaacaata tgtccactcg
     1981 ttttaaggga tttgttgact taagcaaatt tattgagcga gtaaataaag atagtcatga
     2041 tgcggatgct ttttctcgga agctagctaa ggcgtatgct cgaagcgaaa caaagcaaac
     2101 gctacaaaca ataacaaatg aacatcttaa gagtgttcta gatgaaaatg gatatttagc
     2161 aggtgtttta aagtataaaa aaggtgatgc tacgatttgc atcgcaagcc ataaccagca
     2221 agatacgttt atcgctatgg tatccgaagg cgtattgaga tcgttgatta caggcattga
     2281 ttatttaagc cttgggtcaa agcggaaaat atcgtcagca gcatccaata ctactaccag
     2341 taccacttcg gattcctctt ccgctgccgt gtgagacaaa acaacctata tcagataaca
     2401 gccctgcttt tgcggggctt tttgtttgtg cgtgatgcga cctcgatgta cgatagatgc
     2461 tgtactaagc cagtacacac agcgtcccct cttcgaggtg ccgtctgtga ctgtttcagg
     2521 gggctcgccg ccccccgaaa ccccttgcat ccactgcgaa aattcgcact ttgggtgcga
     2581 aactttctca gcggattctc atggaaaagc gcaccaaaga gatcaaaatc agactcaccg
     2641 aagcggagca tcagcggcta cttgaacgct gtgaccgtaa gcatttggcc gagtggttac
     2701 gagccgttgg cttaggcgaa tcgcgtacag ctcggcgtcg tccgctacct accgtagacc
     2761 cgaccttgtt acgtcaggtc agcgggatcg gtaataacct caatcaaata gcccgttact
     2821 tgaatcagca tggcttaccg ccgcaagaac gggtgtcgtt gttagcggtg ctcaatagca
     2881 ttgaccaaca tcttgccgaa ctgctggagc aacatcgtga tcgttaagat tcatggtcgt
     2941 ggtgccggtg gcgggagtgg tcctgtcgat taccttctgg gccctgatcg tcagcgcgaa
     3001 caagcgacgg tgttacgggg taaccctgag cacgtcaaag agctgattga tggctgcgaa
     3061 tttgcccgaa cttatacctc tggcgtgctc tcttttcagg agagtgattt acccgaaggc
     3121 gaaaagcagc gattgatgga tgaatgggag cagacattga tgaccggtct agataaagac
     3181 cagtatgcct gcctctgggt tcaacatcag gacaaagggc gtcttgaatt gaattttgtt
     3241 atcccgaaca tcgaattgca gagcggaaaa cggctacaac cttactttga tcgggctgac
     3301 cggcctcgtg ttaacgcatg gcaaaccctc accaatgacc ggcttgggtt acgcgacccg
     3361 aatgaccctg ctaatcgccg agcattaacc ccctctaatg accttcctcg caacaaacag
     3421 caggcagcgg aagccattac caaagggcta atcagcttga ttgagcaggg agaaattacg
     3481 gatcgtaaag gggtgatttc ccaccttacc gatgccggat tgtcggtcgt acgggaaacc
     3541 aaatccagta tcagtattgc tgatccggca ggtggcccga atattcgctt aaaaggagtg
     3601 ctgtatgagc gagattttaa atttagcgcg ggagttcgag agcaaatcga agcagcaagc
     3661 caagactacc gcaacgagcg tcgcgaacgc attcgagaag cacgagaaac gtatcaccga
     3721 ggccttgaaa ttaagctcag ggaacataca gaccgctatc caagaagaga acgacagcca
     3781 gctaaaacag atacaccgct tagtcggaat gacatggctg tacagcctgg cattaagtgg
     3841 gatcctgttt gcgatattga ttggagtagc ttggtatctc gggactatcg tggtcgaacg
     3901 ccagaacgaa atcagcgagc agagccagat cctgcaggac ttaaagagcc agaccggagc
     3961 cggcgtatcg ataattcacg attccaagaa caagagcgtg tattacctga tccttccgca
     4021 gggggcgaag aagatcgacg agtacaagaa cgctcaacat cgtcaggtca tcaagtacag
     4081 cgccaaataa cctcatcaga cgccacagaa tcgattctgg gcggttttat ctatcagggt
     4141 gaagagattc atgaccgaaa tggagcagca gcttctgagc gcattagaga gcttacagag
     4201 gcactacgaa caacagcagc aagcgtggca ggacagctac gccaacttac agcgcatgtt
     4261 cgaggttacc tcgcaggagt tggcgaaaaa cgacagggtt tgtcaggcct tgagcatgca
     4321 agtcaccggc ttggcgcagc aagtcgagag cttaaacaga acagtgcgcc gcttgagcaa
     4381 ttagccaagc ggcacgaaca gcggtattct cgcggtcacg gcatgagttt ataagcgttt
     4441 atcggccgca tcgtaagcgg cagaacgctc gcgcttaccg accgccacca cgaataccgt
     4501 aatggtttga tcgcgaacct gatagaccaa gcgataaccg gatgcacgga gcttgatttt
     4561 gtagcagtca tgcagctctc gcaggcgatt tttatcgatc cgcgggtgtt gtagaacctg
     4621 ctcgagtttt ttcttgaact gcagacggac atcatccccg agcttgcgcc attccttcag
     4681 ggctcgggga tcaaattcaa ggttatagct catccagtga cacctttacg cccgcctgtg
     4741 ggttttccag acgatcccga acgatagcca tcaaatcggc atcatcctcg gtcagcaaaa
     4801 cctgctggaa cggcaaacgt ccgctttggg ccacatattc cagtgtttgg cgcagaacct
     4861 cggacggcgt tacgcccagc ttttccagtg cggcataaga gcggcttttc agctcgtcat
     4921 cgatccgaat attaatcgtg gccatcatct cacctcttga tgtagtgaca agtgtatcta
     4981 caagaagtag tatgagcgta aagccgtgcg agaacaagca ggaataacgg attgtcgggg
     5041 atgacaaaaa ccgttgttga ggtgtaactt agtggcagaa aaaacaaagc cccgaaatca
     5101 tgctctactt tggcgagcgg acatgacatt cagggctagg tcgaaaccta gaaaggatat
     5161 tagcacatgc agcgtgcaaa acaacagccc cgccataagg ctgggagcct tggataatca
     5221 ggctttaacg ctttttaacg accggttacc ccacaagccg tacttctccg atgatttaca
     5281 gtttggtgtt cgcattgccg gtaaagagcg tgctctcctc gcaaaataca tccagtttaa
     5341 tcagcctcac gccatgtact ggctttgctt tgacgtggac agagccggag ccgcgattga
     5401 ttgggccgat ctgggggcac ctgcgcccac actcaccatc aaaaaccccg agaacggaca
     5461 tgctcacctg ttgtatgcct tgcatacggc ggtacgtacc gcgccggatg gtcgagctgc
     5521 cccccttaaa tacgccgccg ccatcgagaa tgcgctgcgt aaaaaattgg gcgcagatgc
     5581 ggggtattca gggctaattt gcaagaatcc gaaccacctg cactggcaga tcaccgtctg
     5641 gcagccagag ctctataccc ttgactggtt agccgattac ctcgaccttg gcgcagccaa
     5701 tgaccgcgaa atcctgccgg actacggttt aggccgtaac tgcaccctct tcgataaaac
     5761 ccgcaagtgg gcttaccgcg ctatccgcca aggctggccg cagtatgacc aatggctaca
     5821 agcctgcatt gaacgcgcta aagcctacaa cctgcagttc tccgcacctt tagacgagaa
     5881 cgaagtcatg ggaattgcta aaagtgtggc taagtggaca agtaagaacc ttacagagct
     5941 tggctttgaa gagtatgtta aacaaacaca tacttctgaa attcagcgtc aacgtggaaa
     6001 aaaaagctct ggtggtggaa ggccgaaagt tagagatgga gaatggattt ctttaggaat
     6061 aagtagatca acgtggtaca ggaaatttta caaaaatgaa aattagaatg tatctcttat
     6121 caatgttgtt tttatttata ccgataacaa tatatttttc tggtattgat aacgtatggt
     6181 ctgttttaga taaggccatg tcttcgtgtt ctggcgcgga tggaaaattt caatgcgtac
     6241 tcgactattc aaatagcaga ttaacatttt ctgtattgag gtcagtgatc tgcggggctt
     6301 tgattttaac ttgttcttat acatacattt ggtatcgatg ggttttgaaa aacttagact
     6361 ccggtgcggt gtcaagaaag ttaaaggtcg tttcatgtaa aaaagaatcc ggtgagtatc
     6421 tttctttttt atcaacgtac ataatgccgt tggtctttac tgatttaagc aaaccatcaa
     6481 atatagttaa ttttttgttt gttttgatta tcgtcggttt tttgcacatc aaaacaaaaa
     6541 gaattcactg caacccaaca ttgtcattat ttaatgtttc tgcctataag attacttaca
     6601 gtgtggtagc taatggaagg gaaaaaacgc aagacggtga gttgatagtt ttatctaaag
     6661 atttaataaa agaaaatgat tttatcagag ttataaatca tgatgattat ttaacgttcg
     6721 cgaaaaaaat catagaggaa tgatgatgag tgacgatgcc ttagatcttg atgctttgga
     6781 gcataatgaa gaaaaagcac aagagctttt agaactgtat aagcattttg attgtgatat
     6841 taacgatgtt acattgcaca cttactttaa ctttaaaaat aaaggtaaag cgggtgctaa
     6901 ggactctttt agctataaaa ttgccatcct taaaaatgac agtgagctaa aacagtccat
     6961 tattgataat gtgataaaga aaactatatc tgatttagaa aatcgggttg tgatagtatc
     7021 agatgttaat acactaagtg accgcaccaa caccatttca tttttaaatg atgctgagca
     7081 gtatgataat gagcaaatga agtttctgac tggcattgtg tttgatgctg ggtttggtgg
     7141 tgatggcaat gaaaatatcc caattgttga ttttgacagt gtggatctaa tatcaaaagt
     7201 tgactcaatc attttcgttt tgagtaagaa taatgacgac aattgtataa ccatatacaa
     7261 gaagcargwt cgtctgacgg cagaagaaga ccaggaaatc agaaaaaggg ctgctgaatg
     7321 cggcaagacc gtttccggtt ttttacgggc ggcagctctc ggtaagaaag ttaactcact
     7381 gactgatgat cgggtactga aagaagttat gagactgggg gcgttacaga aaaaactctt
     7441 tatcgacggc aagcgtgtcg gggacaggga gtatgcggag gtgctgatcg ctattacgga
     7501 gtatcaccgt gccctgttat ccaggcttat ggcagattag cttcccggag agaaaactgt
     7561 cgaaaactga cggtatgaac accgtaagct cccaaagtga tcaccattcg ctttcatgca
     7621 tagctatgca gcgagctgaa aacgatcctg acgcatcctt cctgtttttc cggggtaaaa
     7681 catctctttt tgcggtgtct gcgtcagaat cgcgttcagc gcgtttcagt ggtgcgtaca
     7741 attaagggat tatggtaaat atatgagcta tgcgataact ttaactgtga agcgatgaac
     7801 ccattacagg caaagccaat tactcctgac agtggtttag ccagaagcag ggctaccaag
     7861 accaatgcaa taagtaatat atcgttttgc tatcgtgcat cgctcgcgct cagttccatt
     7921 gtgctttttt aagctgtcgt ttttcttacg gtatataccg gttttttatg gcgtggtttc
     7981 ttaacttgtt cagctactga tggacccatt gtatctaggt agtcaactag ctttgttaga
     8041 tcataaatat tgcgaccacc atatcggcga tcactcttcg atgttggttt cttgtccaag
     8101 agattagctt tttcaagatc attgatagct ctctgaacag tccgtacaga aacccccata
     8161 cgtatggcta gactttccat tgacggatgc ggccactctt gcaaactcca ccagtgaacg
     8221 atcaggttaa gtagtgtgtt aaaggccact gaagttagct ttttctcgtt ttgtataaaa
     8281 aacaatacgg taggcactgc tgtccagcca agagacaaac cgccagcttt ccatttattc
     8341 ttaacggagt aagtcattga ttttcctaag ccccaaaata tttaaagtat atattatatg
     8401 gtatattcat atgaataggg tgacactggc gccattattg tgcaaccaaa aaagactact
     8461 ctgaaaacga ggaaaagatt ttttcctgcc tgaattagat acggagttag cgatatgaaa
     8521 accgaacaac gtcatgatct tgttaaagat attgaggttt ttggcgtatc cttgtctctg
     8581 ttgatttcca gagcgaatga gaagtctgtt acaatgccat ctggtctaag tcgggagcag
     8641 agaagagcat gggcagcgga gcaggcgcgc aaaatccaca attgaatatt gtctcattct
     8701 ctgagacctt caacctttat tacacatcca gatattctgc aaaaacactc gataaaatcg
     8761 atgatttcat tgagcatttt gaaaaataca atctctttgg cgatccttta aaaggatatc
     8821 cagcttggac tggcaaagta tcgccatcgt ggaaagtgcc tgatcattac gaaaacaaag
     8881 aagctattga gaagtatgct agagctaaca aattatggca tgctcattta ggcgatccgg
     8941 tttttaaaga tacgtttcat gggaaataca aggtttctga ctgggttatt catttccagc
     9001 ggctgacacc gaaccatata aagcttttag agcttggata ccatgaccca atgaagctac
     9061 ctcaaaactt tgaatgatcg agcggcaggc taaatgaaat cttgagattc attcagtctc
     9121 gtcgtaatct cactattgta aaaacgaaaa aaccaccctg gcaggtggtt tttcgaaggt
     9181 tagttaatcc tggcagattc tctaaccgtg gtaacagtct tgtgcgagac atgtcaccaa
     9241 atactgtcct ttcagtgtag cctcagttag gccgccactt caagaactct cgttacatct
     9301 ctcgcacatc ctgcttacca gtggccgttg ccagtggcgt taagtcgtgt cttaccgggt
     9361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9421 gcacacagcc cagcttggag cgaacgacct acaccgaacc gagataccaa cagcgtgagc
     9481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9661 ggcggagcct atggaaaaac gcctcccgcg cgcccttctt ctgggtcttt gtcttttgct
     9721 cacatgttct ttccggtttt atcccccgat tctgtggata accgtattac cgcctttgag
     9781 tgagctgaca ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa
     9841 gcggaagagc gccttatgtg acattttctc cttacgctcc tctatgccgt tctgcatcct
     9901 gtccggatgc gttatatccc ggtaagattt tccgcttcaa agcgtgtctg tatgctgttc
     9961 tggagttctt ctgcgagttc gtgcagtttc tcacacatgg cggcctgttc gtcggcattg
    10021 agtgcgtcca gtttttcgag cagcgtcagg ctctgacttt ttatgaatcc cgccatgttg
    10081 agtacggctt gctgctgctt attcatcttt tcgttttctc cgttctgtct gtcatctgcg
    10141 ttgtgtgatt atatcgcgta ccacttttcg actgttttgc tgccgctatt ctgccgcttg
    10201 gctttttgac gggcatttct gtcagacaac actgtcactg ccaaaaaact gccgtgcctt
    10261 tgtcggtaat tcgagcttgc tgacaggaca ggatgtgcaa ttgttatacc gcgcatacat
    10321 gcacgctatt acaattgccc tggtcagggc tttgccccga cacccatgtc agatacggag
    10381 ccatgtttta tgacaaaacg aagtggaagt aatacgcgca ggcgggctat cagtcgccct
    10441 gttcgtctga cggcagaaga agaccaggaa atcagaaaaa gggctgctga atgcggcaag
    10501 accgtttccg gttttttacg ggcggcagct ctcggtaaga aagttaactc actgactgat
    10561 gatcgggtac tgaaagaagt tatgagactg ggggcgttac agaaaaaact ctttatcgac
    10621 ggcaagcgtg tcggggacag ggagtatgcg gaggtgctga tcgctattac ggagtatcac
    10681 cgtgccctgt tatccaggct tatggcagat tagcttcccg gagagaaaac tgtcgaaaac
    10741 tgacggtatg aacaccgtaa gctcccaaag tgatcaccat tcgctttcat gcatagctat
    10801 gcagcgagct gaaaacgatc ctgacgcatc cttcctgttt ttccggggta aaacatctct
    10861 ttttgcggtg tctgcgtcag aatcgcgttc agcgcgtttc agtggtgcgt acaattaagg
    10921 gattatggta aatatatgag ctatgcgata actttaactg tgaagcgatg aacccattac
    10981 aggcaaagcc aattactcct gacagtggtt tagccagaag cagggctacc aagaccaatg
    11041 caataagtaa tatatcgttt tgctatcgtg catcgctcgc gctcagttcc attgtgcttt
    11101 tttaagctgt cgtttttctt acggtatata ccggtttttt atggcgtggt ttcttaactt
    11161 gttcagctac tgatggaccc attgtatcta ggtagtcaac tagctttgtt agatcataaa
    11221 tattgcgacc accatatcgg cgatcactct tcgatgttgg tttcttgtcc aagagattag
    11281 ctttttcaag atcattgata gctctctgaa cagtccgtac agaaaccccc atacgtatgg
    11341 ctagactttc cattgacgga tgcggccact cttgcaaact ccaccagtga acgatcaggt
    11401 taagtagtgt gttaaaggcc actgaagtta gctttttctc gttttgtata aaaaacaata
    11461 cggtaggcac tgctgtccag ccaagagaca aaccgccag
//
