LOCUS       NZ_CP022487             5121 bp    DNA     circular CON 19-FEB-2024
DEFINITION  Enterococcus faecalis ARO1/DG plasmid pARO1.4, complete sequence.
ACCESSION   NZ_CP022487
VERSION     NZ_CP022487.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07371835
            Assembly: GCF_002814115.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis ARO1/DG
  ORGANISM  Enterococcus faecalis ARO1/DG
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Taiaroa,G., Knottenbelt,M., Carter,G., Williamson,D. and Cook,G.
  TITLE     Complete Genome of a New Zealand Enterococcus faecalis Isolate
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Taiaroa,G., Knottenbelt,M., Carter,G., Williamson,D. and Cook,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2017) Biochemistry, Otago University, 362 Leith
            Street, Dunedin 9016, New Zealand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUL-2017
            Assembly Method                   :: Canu v. 1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/19/2024 01:24:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,803
            CDSs (total)                      :: 2,726
            Genes (coding)                    :: 2,688
            CDSs (with protein)               :: 2,688
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 21 of 38
            Pseudo Genes (incomplete)         :: 16 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022487.1.
            Bacteria and source DNA available from Cook Lab, Department of
            Microbiology, The University of Otago.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Enterococcus faecalis ARO1/DG"
                     /mol_type="genomic DNA"
                     /strain="ARO1/DG"
                     /isolation_source="Dog with mastitis"
                     /db_xref="taxon:565651"
                     /plasmid="pARO1.4"
                     /geo_loc_name="New Zealand: Otago"
                     /lat_lon="45.8647 S 170.5144 E"
                     /collection_date="2001-06-05"
                     /collected_by="Department of Microbiology, The University
                     of Otago"
     gene            177..1856
                     /gene="mobV"
                     /locus_tag="CG806_RS00935"
                     /old_locus_tag="CG806_00920"
     CDS             177..1856
                     /gene="mobV"
                     /locus_tag="CG806_RS00935"
                     /old_locus_tag="CG806_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_010717414.1"
                     /translation="MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQNLLEIQEELPT
                     YLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQILAEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANVKEKKFSLNGLK
                     YVLSASDFSAIKSITKDFETLRTQNKHLKIENNNLAQKNKELTQVNHEQELQITELAYE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDTTTQTVSVLETENKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGHAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="mobV"
     gene            1945..2145
                     /locus_tag="CG806_RS00940"
                     /old_locus_tag="CG806_00925"
     CDS             1945..2145
                     /locus_tag="CG806_RS00940"
                     /old_locus_tag="CG806_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364281.1"
                     /translation="MKKLLVLILGVSFISVALSSTTEASAREWDSIECQPGFYWKKGGW
                     TAGGYHKAGGFCIPMHTRWTK"
                     /name="hypothetical protein"
     gene            complement(2664..2750)
                     /locus_tag="CG806_RS14705"
     CDS             complement(2664..2750)
                     /locus_tag="CG806_RS14705"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107196852.1"
                     /translation="MIESLISLIIAPLFVGLVLLLVDRWLDD"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3375..4097
                     /locus_tag="CG806_RS00955"
                     /old_locus_tag="CG806_00940"
     CDS             3375..4097
                     /locus_tag="CG806_RS00955"
                     /old_locus_tag="CG806_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002360724.1"
                     /translation="MSDKSELAVKYQNELNLVPLKNFNAKQMDLFFALCARMKDKGVEN
                     IRFSFEELKELSDYKMTATKAFVADLEKLYKDMLNLSYRTENDDEIEYFVLFNGFKIDK
                     KQKFVEVRVNQDLDYIINGLTTEFSRFELSAFTSIRSTYAKTLFRLLMQYRSTGYYVVS
                     IEDFRELLDIPEYYQMGNIDQKVLKPAMKELHNYFENLEVTKIKAKKGNKIAKLEFTFT
                     GLKTNKPSVTMHDWVNGE"
                     /name="replication initiation protein"
     gene            4224..4520
                     /locus_tag="CG806_RS00960"
                     /old_locus_tag="CG806_00945"
     CDS             4224..4520
                     /locus_tag="CG806_RS00960"
                     /old_locus_tag="CG806_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002360723.1"
                     /translation="MGLMTGYNMTKEEYEVTQAVLERLDKFESELEALLLDLEPLDLSR
                     LDTVVRDETLRMFNYLSRSYILLIDTVAYKVTLEEDISCGIVKIDGQSLDEEE"
                     /name="hypothetical protein"
     Typing          3376..4097
                     /name="rep_cluster_2301"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          103..1853
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atcggctaag tgcgagattg ggaagcctaa aggctgacct ttcgagtact agccgacagc
       61 cctcggcaga gcgcaccttt acgaagtaaa gtatagtgcg ttatacttta cttggaagta
      121 ctgccgaaat ccaaagacac cgaacgacac aagaaaaaaa agggggagga gcgctcatgt
      181 caaaaattgt tgcacgtatg gaaaaaatga aagacggaaa tttaagtggt atccaacgac
      241 ataatcaacg agaaaccaat aatcattcca atcctgatat tgatattgag aaatctcact
      301 tgaattatga cttagtcaat cctggttcaa tcaattatcg ggagaaaatc aaacaaatca
      361 ttgagagcca acgaatcagt aaacgagcgg ttagaaaaga cgcagtcctt gtgaacgaat
      421 ggataatcac tagtgatacc gccttttttc aagagaatac agacacacaa gcatttttta
      481 ccgatgttgt cgcatatttc tctgatcgct gcggtcgaca aaatgtcgcc tatgccacgg
      541 tacatttaga cgaaaccacg ccccatatgc acttaggaat tgtgcctatg tacgaagggc
      601 gattgagcag taaacaggtg tttagtcggc aaaatttgtt agagattcaa gaagaattgc
      661 caacctattt gcaagaacga ggctatgcta ttgagcgtgg actccgtggg agtccgcaaa
      721 agcatttatc tgtcaaagaa tacaaagacg ttcaaaagga gctacaagtt tctaagcagg
      781 aactcgctaa tcaggaacaa attctggcag aaaaagagag agaactccat tcttatgaac
      841 aggcaatctt ccgtattgac cgccttcttg aagagaagaa cgaagaagta aagggcaaag
      901 tccaagcaat gaaccaactg gaaacgaagc gaaacgaaat cattaactct ctacaaacaa
      961 ccgttttacc tgatttagca aatgtcaaag aaaagaaatt tagtttaaac ggtttaaaat
     1021 acgttttaag cgcttctgac ttttcggcga taaaaagtat tacgaaagat ttcgagacgc
     1081 ttagaacgca aaataagcac cttaaaatcg aaaacaataa cttggcacag aaaaataaag
     1141 aattgacaca agtaaatcat gaacaagaat tacaaataac ggaattagcc tatgaattaa
     1201 ccctgaaaaa agaacaatta acagcgtttg aagaaaaaca actaaactat gaccagagcg
     1261 aacaaatcaa acaagaactt gatacaacaa cacaaacagt ttctgtgtta gaaacagaga
     1321 ataagcggtt aaaagataaa attactgact taacctacga cttttctgaa ctaaatgaaa
     1381 aatatcaaga aataaaatta aagtttgacg ggttaactac ttaccttcac gaaagactag
     1441 gtcatgctaa agaaaccatt ttatttacga ttcagcaagg agtgaagtac cttcagaacg
     1501 cgcctaaatt aaaaaaatcc ctagagcgat attccaagga tagaacttac ctcattgacg
     1561 aaaaagggac attgttttta ccaccagata cggggtcaaa aggggtaaca atgtccttaa
     1621 acccacattt ctacccccta aaagccccgt tttacaacag agaaacaggg tgttatgagt
     1681 ttgtcggcga atatatggct aatggacgtt ctcgaacgaa tactttacaa ctgtctgaag
     1741 aacaagtgct agagaagaaa gcttttacgt ttgaacagtc gacagataaa aaggcttggc
     1801 aacagagtaa aaaatattca agagatatgt caaaaggacg aggtttatct cgttaaaaat
     1861 aatttaattg ataaattatt gacacataaa aacagttaaa ttaaaatata ttaataaaaa
     1921 ttaaaaaaaa gtgaggtaag cattatgaaa aagttgttag ttctaatttt aggggtgtct
     1981 tttatctctg ttgctttaag ttcaactaca gaagcaagtg ctagagagtg ggattctata
     2041 gagtgtcagc caggatttta ttggaaaaaa ggcgggtgga ctgcaggagg atatcataaa
     2101 gccggtggat tttgtatccc aatgcataca agatggacaa aatagttgct aaatttgagg
     2161 ttatttcatg aaaaaatatc taataaaaaa tatattttat attactattc ctttagtttt
     2221 aagctatata acttcttttt tagttaatat tgatttgccc atattgataa ttatttttta
     2281 cggaatttta ttattttttt taattccttc agaagtatat ttgggaagta caatggatta
     2341 caacgcgaaa gtagtgaatc ccacttacag accagaaaat aaaagctttg aagatagtcc
     2401 aaaaagtaaa atattgtcta tcctcatagt attactatgt ttaatactaa ctatttcgat
     2461 atggtatttc tctaattgac gttttgcgta atctaatctg atgtttgaaa atacagaaaa
     2521 aaaatgttat tgtgtaagtg cgaagcgaaa ttaagcaaaa tcatgtttac accaaaaagc
     2581 cctcctattc tcagtaggag ggctttttta cactgttgcg atttcagcgt tggagctaga
     2641 aaagcaactg catagattat ctgctaatcg tctagccaac gatcaactag caaaagtact
     2701 aaacccacaa aaagtggggc gataatcaac gaaattaagc tttcaatcat gtttacaccc
     2761 cctttcgggg cagttgccaa aacaagtata tcacaagaag aatttcttgt gggggttgtt
     2821 agggcttgcc ctgaccgcct gcaaggcgca atcctaacca agatacccta aaacaactaa
     2881 acatgtctta aagatcttca gacaagcaaa tagagcctaa ccaaatgatt taccttgtca
     2941 acggtattga ttttgatttt agcgagtgaa cgaagtgaac gctgcaagca aaatgtgagc
     3001 gggattttcg ctcactcctt ttttgatttt ttagtgccag ttttgtaaat atcgcggacc
     3061 tgtttgtaga aaaaagaggt taattttggg cgggaaattt ccgcgcgcgc acgcgtttct
     3121 tttatatata ttttatatat atttttaaaa cctttttagg cgggctgtgt gccacgtggc
     3181 tctaaggaag agcagatggt accaacaact ttttatctga ctaccaacaa ctttttatct
     3241 gactaccaac aactttttat ctgactacca acaacttttt atctgtttta ttttcataat
     3301 attaaaacaa acaaccaact gttgacaaag ttggttgttt gttttaatat tcgaattgtg
     3361 agggggcgaa aggaatgagt gataaatcgg aattagctgt taaatatcaa aatgagttaa
     3421 atcttgtgcc acttaaaaac tttaatgcaa aacaaatgga tctgttcttt gcgttatgcg
     3481 cacgaatgaa agataaagga gtagagaata ttcgtttctc cttcgaggaa ttaaaggaat
     3541 tgagtgacta caaaatgact gctacaaaag cctttgtagc agacttagaa aagttgtata
     3601 aagatatgct gaacctttct tatcgtacag aaaacgatga cgaaatagag tattttgttt
     3661 tattcaatgg atttaaaatc gacaaaaaac aaaagtttgt tgaagttcga gttaatcaag
     3721 atttagacta tataataaat ggtttaacaa cagagtttag taggtttgag ctgtccgctt
     3781 ttacttcaat acgctcaaca tatgctaaaa cattgtttcg tttgttaatg caatatcgtt
     3841 caacagggta ttatgtagtc agtattgaag acttcagaga attgttagac atacccgaat
     3901 attatcaaat gggtaacatt gaccaaaagg ttttaaaacc agcaatgaaa gaactccata
     3961 actattttga gaatttagaa gtaacaaaaa tcaaagccaa aaaaggcaat aagattgcca
     4021 aactagaatt tacgtttact ggtctgaaaa ccaacaaacc aagcgtaacc atgcacgatt
     4081 gggtaaacgg agaataaaaa aagaacttgc cccctcacat taagcaagtt agcaagttcc
     4141 tcacgcaatc ttccggactg ctagattatt ttagcatatt tgcgtgagga cttctaaaat
     4201 aaattatttt ggaggtccta cttatgggat taatgacagg atacaacatg acaaaagagg
     4261 agtacgaagt cacacaggct gtactggagc gactagataa gtttgagagt gaactagaag
     4321 cgctattgct tgatttggag ccacttgact tatcacgatt agatactgta gttcgagacg
     4381 aaacgctaag aatgtttaat tatctaagtc ggtcatacat tctacttatt gatacggtag
     4441 cctacaaagt tacgctagaa gaagatattt cctgtgggat tgtgaagatt gacggtcaat
     4501 ctctagacga agaagaataa aaactactct tgcttgttat aaaaaaatgt tgactatttg
     4561 ggataacact cgaaaaatat caagattagc ctacttccat tttggggtag gctttctttt
     4621 tgcaagcgga gcaacatggt accatgttgc gatattttga acgactccta ccaaaaatac
     4681 tggtaggagt cgttcaaatt tttggggttc ccccaatccc actgctgtta ttttttgctt
     4741 ttactgttga aattcgctta atttcttcct gtgtttcgga agagcataag gggaactggg
     4801 gggcacacaa ggtaccccgc cttgcactgc aaggctttcc cgtggggtac actttcgttc
     4861 ttcgctagca ctttactagc aggtactttc aggtaaacta gcacattttt agcactcaat
     4921 agcaggtttc tagcacccaa actagcagat tcctagcagt gactagcagg tttttagcac
     4981 caaattagca cattactagc accggctagc agaaagctag cacgttcact aatttcaggc
     5041 agtcgttatt taaacggttt tctgacggag ggaaatttgg agtttctgcc ttagcagaaa
     5101 tggaaagttt gaccgtaggc a
//
