LOCUS       NZ_CP022075             9293 bp    DNA     circular CON 20-DEC-2023
DEFINITION  Citrobacter koseri strain FDAARGOS_287 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP022075
VERSION     NZ_CP022075.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06173300
            Assembly: GCF_002208985.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri
  ORGANISM  Citrobacter koseri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Vyas,G., Nadendla,S.,
            Aluvathingal,J., Geyer,C., Nandy,P., Hobson,J. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Vyas,G., Nadendla,S.,
            Aluvathingal,J., Geyer,C., Nandy,P., Hobson,J. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 9293)
  AUTHORS   Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Nadendla,S.,
            George,J., Geyer,C. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 2.3.0, HGAP v. 3.0
            Genome Coverage                   :: 667.045x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/20/2023 01:26:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,712
            CDSs (total)                      :: 4,597
            Genes (coding)                    :: 4,545
            CDSs (with protein)               :: 4,545
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 24 of 52
            Pseudo Genes (incomplete)         :: 32 of 52
            Pseudo Genes (internal stop)      :: 10 of 52
            Pseudo Genes (multiple problems)  :: 11 of 52
            Pseudo Genes (short protein)      :: 1 of 52
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022075.2.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Citrobacter koseri"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_287"
                     /isolation_source="Clean catch urine"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_287"
                     /db_xref="taxon:545"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: DC"
                     /collection_date="2014-11-04"
                     /collected_by="Children's National Hospital"
     gene            152..2116
                     /locus_tag="CEP66_RS24325"
                     /old_locus_tag="CEP66_24255"
     CDS             152..2116
                     /locus_tag="CEP66_RS24325"
                     /old_locus_tag="CEP66_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            2116..2847
                     /locus_tag="CEP66_RS24330"
                     /old_locus_tag="CEP66_24260"
     CDS             2116..2847
                     /locus_tag="CEP66_RS24330"
                     /old_locus_tag="CEP66_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            2854..3384
                     /locus_tag="CEP66_RS24335"
                     /old_locus_tag="CEP66_24265"
     CDS             2854..3384
                     /locus_tag="CEP66_RS24335"
                     /old_locus_tag="CEP66_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3412..3591)
                     /locus_tag="CEP66_RS24340"
                     /old_locus_tag="CEP66_24270"
     CDS             complement(3412..3591)
                     /locus_tag="CEP66_RS24340"
                     /old_locus_tag="CEP66_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3660..4037
                     /locus_tag="CEP66_RS24345"
                     /old_locus_tag="CEP66_24275"
     CDS             3660..4037
                     /locus_tag="CEP66_RS24345"
                     /old_locus_tag="CEP66_24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(4099..4398)
                     /locus_tag="CEP66_RS24350"
                     /old_locus_tag="CEP66_24280"
     CDS             complement(4099..4398)
                     /locus_tag="CEP66_RS24350"
                     /old_locus_tag="CEP66_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4388..4651)
                     /locus_tag="CEP66_RS24355"
                     /old_locus_tag="CEP66_24285"
     CDS             complement(4388..4651)
                     /locus_tag="CEP66_RS24355"
                     /old_locus_tag="CEP66_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(4957..5376)
                     /locus_tag="CEP66_RS24360"
                     /old_locus_tag="CEP66_24290"
     CDS             complement(4957..5376)
                     /locus_tag="CEP66_RS24360"
                     /old_locus_tag="CEP66_24290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            5778..7463
                     /locus_tag="CEP66_RS24370"
                     /old_locus_tag="CEP66_24300"
     CDS             5778..7463
                     /locus_tag="CEP66_RS24370"
                     /old_locus_tag="CEP66_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7473..7730
                     /locus_tag="CEP66_RS24375"
                     /old_locus_tag="CEP66_24305"
     CDS             7473..7730
                     /locus_tag="CEP66_RS24375"
                     /old_locus_tag="CEP66_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            7815..7964
                     /locus_tag="CEP66_RS24380"
                     /old_locus_tag="CEP66_24310"
     CDS             7815..7964
                     /locus_tag="CEP66_RS24380"
                     /old_locus_tag="CEP66_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             778..9293
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(8068..8646)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2117..2844
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 atggggcgga aagaggctgg cctccgtggt gaaagctgaa cgcaatcacc acggacgtga
       61 ccgccgccgc catgccttgg ggcatgtccg cggcttgtgc agcgggggct gcacatcacg
      121 ccggcaaacc atcgtctgaa ggagagagaa catgtttaat acggattcgc ttgcctggaa
      181 gtggcacatt ccggagctgg cccggcagat ttatccgtgg gtcagctggc tgtaccagga
      241 gtattacggt ctggccgggg ctttcctagt tatcggcctg tttttcggtc tggctgaact
      301 ttgggccgga cggatgcgtt ttgtggggct gagtcataag gagcgacggg agtatgccga
      361 tcgctataag cgcgataata aacggccagc gccattttcg gtgctggcct cgctggattc
      421 gcccgggcgg atggtcaggg cacgtcgaca tgtctggaac tacgcgtact ggctgaccgg
      481 cgcggccggt ctggcccttc tgggggggct ggttgtcgcc gtgtttatcg agagctgggg
      541 agaaaccgtc ctgcgtccgg acctctacgc aaacgcagat tattacctgc tgcagccggc
      601 taacctgttc gggattgcct ggtgcgcggg cgggctggct gtcgggattg tgttggcgcg
      661 gctgctggcg ttcctgctgc ttgacggtca ttttgcggct gctgacgagg cggtgaacgc
      721 gaaactgaat caggagagcc ggcgcagcag ccagcggaca ggagagatga ccgacgtgcg
      781 ccacctgcat ttcggggagc cggtaccggt taatgcgctg gccgattttt cctcggagca
      841 ggcccggcag gagcaggcgg tgtttctggg taaggatgag cagggcaagc cggtgctggt
      901 tccgcgtgat acctggcgca agaccaacat tcagatcctc ggcctgcccg ggagcggcaa
      961 gagcgtgatg gccaccaacg cgctcattcg ctgcgtacgc gatttcgggg acgcggtggt
     1021 gtacttcgac cccaaagggg acgcatgggc gccgcacgtc ttccgggcgc actgtccgga
     1081 cttcaccctg ctggatctgc ggccggggaa accggcgcag ctgaatctgt tccgcgatct
     1141 cgaccagtac gcgctgaaaa acctgctgat tgccgggctt aacctgggcg agaccggcga
     1201 cgtggcggac cactaccgca tcagcgagca gaaagcggcg aagctgattg cggagcagtt
     1261 tccgcagggg gcaaacattc agcaggtgct ggcggcggcg tacgcgctgc cggaggccct
     1321 gaaaaaggac gtgaaggggt tgatcactag gctggaaaac gtcgctgacc tgagcgtcct
     1381 gcagacggac agtggtatcg atgtggcggg gattatcaac ggcggcggct gcctgtacgt
     1441 tatcggctcg atggacgacg aggcggtgat ccgtgtgcaa aaaatgctgt ttgcccgctg
     1501 cgcgcaaatc atcattgcgc gggacgagtt ccgggagtgg ccacacgtca gcgtgatgct
     1561 cgatgaaatt aaatatctgc tgtcgaagta tgtactgaac gcactgggga cgctgcgcag
     1621 ccgggactgc aatctgttgc tcgcgcacca gtcgctgggc gatttcgggc agtgcgggca
     1681 ggatctgccc gctgaatttg tgaaaaccac ggtactggat aacacgccga tccgctggtt
     1741 ttaccgggcg gccagcctgg agtccgcgca gtgggcggcg gggcagaccg gggaaatcag
     1801 ggtggacgtg gagcgccgcc gtgccagccg ggaggcgggg aacgtggagc atatcagcgg
     1861 ggacagcttc atccagaagg aggcgcggcc gctgtttgac gtgaacaccc tgcaacacct
     1921 gccggacggg ttcgcggtca tgaccgggct gggtgtggcc cggatggcgt tcagcagtcc
     1981 gctgcgggtt gagcgccggg aaatcccgtt gcagcgtttc ccggtgctgg cgaaaaccga
     2041 tcccctggcg gagttccggc ccccggccga cggcggcggg ccgaaagcgg atgatgattt
     2101 tgcggggatt tactgatggc gctggagctg tacaacgtca gccacgcgaa acgacaggcg
     2161 cggaacgcgg agaaaacccg gctgaccctg cgctggttgc gggaagagct gtgttccacg
     2221 gcggagctgg ttgcgcggcg gctggggatt gcggcggtgc agccggtcta ccgcttcctc
     2281 gacagcctgg tggcgaaagg gctgctggtc cggaccaaat atccggtgga cggccgtcag
     2341 gtcagcgtgt gggggctgac gccgcatggc gtggcgttct cgttcgacga ggacgaaccg
     2401 ctggcggatg ttattccgtt tcagccgtca agggtgtcgg cagcacatct gccgcaccgg
     2461 ctggccgtgc aatccctgcg ccttgatatg gaagccagag gggccaccgg ctggcgcatt
     2521 ctgcaccgtc tgagtcttaa aggcatgaaa gtgccggacg ccctggcgga gctggacggt
     2581 aaaacggtgg cgtttgaggt ggagcgcacc gtcaagagcc gtcgccggta tcaggaagtg
     2641 gtgtccgggt atctgttcaa ccgcaaagct aacggcatcg atgaaatctg gtacatctgc
     2701 ccggaccggg cgacgcaggt cagggtgcag cgggcaatcc tgtcggtgga tgaaatcgtg
     2761 aacccacaga ccggcgaggc ccgcaaaacg gcagagctgg atcgtgaacg gttgtttgcg
     2821 tgctttaaat ttatgacaac ggagtgaggg attatggcgc tgtacatggt atttattgaa
     2881 gacgcggagg accggaacgg cgagcgctgc atcatgccga ccagcgagca tctggaagat
     2941 atggtggtgc tcgcctggca gaccggggcg tatgttttct gttttgtcga ccgggcccag
     3001 catctggcga tgatgaaatc catcgggatg tatgacgagc ggctcaacag cgacgaaaaa
     3061 gccacgtact ttttcactgg taaaaatccg caatgggaac ggaacaaacc cacaggaatg
     3121 aagctgtgtg attacctcta tcgcaaggtg gagcggttcg ccatcgagaa cggtgtgcag
     3181 gtggttgatg ccgggaagtg ctcaccggag cagattaacg cgatgattga cgtggtgagt
     3241 gagcggcacg gggaaagcgc ggtgaacgag aagctgaaag cggagctgat gcgggaatat
     3301 aacagccgct attttaacgt gccggtacct gaactgccgg aggacaaacc tgcccgccgg
     3361 tggtggccgt tcggcaaaaa ataaatgcca gcaccggcca tgccggtgct gttattcctc
     3421 cccgagctta tcccgcatca cctgccagag tgtttccgca tcctcgtgta gccgctcgca
     3481 catgtcggcc ggggtatcca tatccagctc gttcagttta tcgagcaata gcagcgactg
     3541 ctccttaata aatttagcca tattgagggc tgttttttcc tgttttgtca tgattctttc
     3601 cttccgggca tggtgtgcgc ctgtcaggta tactgtctca aaaatagcgg ggtgagacaa
     3661 tggcgtgggt gacggtgaga caggcgacgg aactgacggg taaagcccga agttccctgt
     3721 atcgggatat ggcgaaaggc cgcgtatcat accgtacgga agctgatggc ggccgggtgg
     3781 tggataccag cgagcttatc cgggtgtacg gtgagttaag acaggatgag actcacgagc
     3841 gggacgagtt gagactgtcg ggtgagactg aaaaaatggc ctccgacgcg ttgatcgcgg
     3901 agataaaagc actcagggag gaggtggccg ggctacggca ggaaatgcag tccatgagac
     3961 tgctggaaca caaaccggag acagcagaga ccccggccgg aacaccgcgc cggtggtggc
     4021 cgtggggaaa acgctgacgc gctgtgcttg tccaaccggc gacggccacg caatgaattg
     4081 cgtccccgtc accggttatt agagaacaat atggggattc tggcgctggc cgaccacggc
     4141 cagaacccgg acttttctgg tctggtcatc aacatcatag aggacatgct gtcccatcag
     4201 actgatccgg cgaacgtcct cgccatagcg tgagtccact tcatagagtc gcgggaagtc
     4261 ggccagtttg tccgccgcag cgagaacccg gtcggtaacg ttctgcgcat cccacaggga
     4321 tgaattttcc gcgatatagt caaacagcgt cgttacatcc tcgatggctc cctgcttcca
     4381 ttccacgtca tacggcatcg cgcagttcct gataacggcc atgcaggcgc tggcggagtt
     4441 gttccgtggt catacccgga cgcgtgtcag cacgggcggc ggtcactttg gcgcgaacct
     4501 gatcttcata ctctgcctgg gccagttctt cgttagccag tcgctgcaac agcgcagctt
     4561 tgtccgggcc accttccggc agtatgcggg gcaggctttc agccagttcc cgaagaaagt
     4621 tggcgttttc gtagtgtgta gtgttgctca ttgcggagcc tcccgggtta atgcggcaat
     4681 atgaaggaag tttagcaggg ggcggtctga aagaatatgg tcgtgtcagt caggctgatg
     4741 aagcagaaag tcggtaaaaa agggcttacg gcatccattt tacgtcaaaa catatgccat
     4801 gcagttgcgt gcataggcat gcattaggat aaaatttatc gggcgcgttt ccggcagttt
     4861 tccggggggg ttgttgcctg ttttatcccg tagccgccgg aaacgccctg agcccgtctg
     4921 agcggtgcgc gtaatgacgc gttatggtaa atccacttac tgtctgcctt catagccatc
     4981 gagataaaca gctgaaatgg tgtcagccag tagccttgat tttttcggtg tgaggtcttt
     5041 gctgcggtaa tcggtacccg caaattgtgt tgtgccttgg agtgagttta aaaattcctg
     5101 actgtgaaac tgctgaatgc gttgctgcac atcactccgt gattttccgg tcgctttatc
     5161 ctttgcgccc atatcccaga actgccggaa aaaaggcacc atcattcgtg ttgtgtcagc
     5221 tttctgggtg gaaaaattgg gatcaaaacc gtcatctgaa gcatatacgt aatgccgggt
     5281 atgctgttcc acgctgccct ttggagcaca ggcagtgaga gttgctgtcg ctgttatcat
     5341 ggcgataaga cacagtttgc tgccggaacg tctcatgagc gtctccttac ctttaattct
     5401 tttttctcat tgagttatat atccgcaggc tatcttatca tcagtttctg accgtgttca
     5461 gtgaccgaac aacccgaacc agacccccgt ttcttttcct gtcccggagg gggctttctg
     5521 gtatctcata taatgatttc agtaaaacat actgaggccg gagaacggcg ccgtttgtgc
     5581 tggtttttgt tgcatctgga ggggattgcg catggtgtga gtggctgttt ttttgatcat
     5641 tttcacaaaa acgcaaattt acagacttga catgaaaaac aaggcagcgt aatttttata
     5701 ctgtatataa aaccagtggt tatatgtaca gtattttatt ttagtttatt gttttaaaag
     5761 tcaaagagga aaagtatatg agtggcggag acggacgagg tccgggtaat tcaggtctgg
     5821 gacacaatgg tggtcaggcc agtgggaatg tgaacggtac atcaggtaaa ggtggccctt
     5881 catcaggtgg tggtacggat ccaaacagcg ggccgggctg gggtacgacg catacaccta
     5941 acggggatat tcataactat aacccggggg agtttggtaa cggcgggagt aaacccggtg
     6001 gtaatggcgg taacagcggc aatcatagcg ggagctctgg tggcggacag tcttcggcca
     6061 ccgcgatggc cttcggtctg cctgccctgg ccactccggg ggctgaagga ctggctttat
     6121 ccgtttccgg cgatgcgttg tcggccgctg ttgctgatgt gctggctgcc ctgaaaggtc
     6181 cgtttaagtt tggtctgtgg gggattgcga tttacggagt gctgccttct gagatagcaa
     6241 aagatgatcc gaaaatgatg tcaaaaatcg tgacgtcatt gccggcagac acggtaacag
     6301 agacgccggt cagctccctg ccgctggacc aggcgacggt cagcgtcact aaacgtgtgg
     6361 cggatattgt gaaggacgag cggcagcata ttgcggttgt caccggccgg ccaatgagtg
     6421 ttcctgtggt ggatgcgaaa ccgacaaaac gtccgggggt attcagtgtg tcgattccgg
     6481 gtctcccgtc tctgcaggtg agcgtaccta aaggtgttcc ggccgcgaaa gccccgccaa
     6541 aaggcattat tgctgaaaaa ggtgattcac gtccggctgg ttttacggcc ggtggtaact
     6601 cccgtgaggc cgttattcgt ttcccgaaag agagcggaca gaaaccggtc tatgtgtcgg
     6661 tgacggatgt tcttactccg gcacaggtaa aacagcgtct ggaggaagaa aagcgtcgcc
     6721 agcaggcatg ggacgctgct cacccggaag aggggctgaa aagagagtat gataaagcga
     6781 aagctgagct ggatgctgaa gataaaaata ttgcgacctt aaacagtcgc attgcatcga
     6841 cagagaaggc gatccccggt gcaagggctg ctgttcagga agccgataaa aaggtaaaag
     6901 aggcagaggc aaataaagat gattttgtga cttataaccc gcctcatgaa tatggctccg
     6961 ggtggcagga tcaggttcgc tatcttgata aggatattca gaatcagaat gagaaattaa
     7021 aagctgccca gacatcttta aacgaaatga atgaatcctt atccagggat aaggctgcgc
     7081 tttccggggc gatggagagc cggaaacaaa aggagaaaaa agcgaaggat gcagaaaata
     7141 aattaaatga ggaaaagaaa aaacctcgta agggaactaa agattacggc catgattatt
     7201 ttcctgatcc caagactgaa gatattaagg ggttgggaga gttgaaagag ggtaaaccta
     7261 aaacccctaa acaaggtggt ggtggtaagc gggcccgatg gtatggtgat aaaaagcgta
     7321 aaatttatga atgggattcc cagcacggtg agcttgaagg gtaccgcgcc agtgatggcg
     7381 aacacctcgg ggcattcgat ccaaaaacgg gtaagcaggt taaagggccg gatccaaaac
     7441 gaaacattaa aaaatatctt taagaggtaa atatggggct taaattacat attcattggt
     7501 ttgataagaa aaccgaagag tttaaaggtg gtgaatactc aaaagacttc ggtgatgatg
     7561 gttctgtcat tgaaagtctg gggatgcctt taaaggataa tattaataat ggttggtttg
     7621 atgttgaaaa atcatgggtt tcgatattac agccacactt taaaaatgta atcgatatta
     7681 gtaaatttga ttactttgta tcctttgttt accgggatgg taactggtaa gcaaaagtta
     7741 tcaaggatga tttgaaatac agacttccgg tgttcaggga tgaatgccgg ggtttttaaa
     7801 tacagggaag gtatatgaaa aaggtaaagg ttgtttttct ttttatattg attttttcag
     7861 gctttcttct ggtcgcatgt caggcaaact atatccggga tgttcagggt ggaacggtgg
     7921 caccatcgtc ctcctctgaa ctgacgggga tcgcggttca gtagaaaaga tcaaaggatc
     7981 ttcttgagat cctttttttc tgcgcgtaat cttttgccct gtaaacgaaa aaaccacctg
     8041 gggaggtggt ttgatcgaag gttaagtcag ttggggaact gcttaaccgt ggtaactggc
     8101 tttcgcagag cacagcaacc aaatctgtcc ttccagtgta gccggacttt ggcgcacact
     8161 tcaagagcaa ccgcgtgttt agctaaacaa atcctctgcg aactcccagt taccaatggc
     8221 tgctgccagt ggcgttttgc gtgcttttcc gggttggact caagtgaaca gttaccggat
     8281 aaggcgcagc agtcgggctg aacggggggt tcttgcttac agcccagctt ggagcgaacg
     8341 acctacaccg agccgagata ccagagtgtg agctatgaga aagcgccaca cttcccgcaa
     8401 gggagaaagg cggaacaggt atccgggaaa cggcagggtt ggaacaggag agcgcaagag
     8461 ggagcgattc atcggaaacg gtgatgatct ttaagtcctg tcgggtttcg ccactcctga
     8521 ctgattcatg gttgagccac cggctcccac agatgcaccg aaaaagcgtc tgtttatgtg
     8581 aactcagtca ggagggcgga gcctatggaa aaacgccacc tgcgtggcct tgccgttttg
     8641 cctcacatgt tagtcccctg cttattcacg gaatctgtgg gtaactttgc atgtgtccgc
     8701 agcgcccgcc gcagtctcac ggccggagcg tagcgaccga gtgagcgagg aggcgcaatt
     8761 tccccggcac ctacaagacg gacaccggac gcgctgatgt ggcatattgt cccctgatta
     8821 agtctttata ttccctgata ataaattacc aggataggtg aatcatatcg gtttttcgct
     8881 gaacatttta cgttaaccta tctctgttgc ttttttctcc ttacggagaa atacaggtca
     8941 tccccgcgag ggcgtggctg acctcagggg gatatcccct gcttacgctg gctggacgcc
     9001 accgacgcac ggtctctccc cctctttctg gctccgtccg gctggacgcc ggccacgcca
     9061 gaaattcacc cggcccccgt caggctcacg cctgaccacc cacaaaaatc cacggtgctg
     9121 gacgcacctg gcgaagcagc aaagctgctc ctgattttgc gggtggtcag ccgctcgcta
     9181 tggcgaactc tgacgggggc cggaacaccg gaaaagaccg cactggggtg ccgccttttc
     9241 ctctcacacg ctggggcgtg ctcgttgttc ctccggggct gcgccttttc cgc
//
