LOCUS       NZ_CP022065            11366 bp    DNA     linear   CON 07-JAN-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Typhimurium var. 5-
            strain FDAARGOS_312 plasmid unnamed5.
ACCESSION   NZ_CP022065
VERSION     NZ_CP022065.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06173325
            Assembly: GCF_002208965.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium var. 5-
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium var. 5-
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11366)
  AUTHORS   Zhao,S., McDermott,P., Tallon,L.J., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Vyas,G., Nadendla,S.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11366)
  AUTHORS   Zhao,S., McDermott,P., Tallon,L.J., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Vyas,G., Nadendla,S.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 11366)
  AUTHORS   Zhao,S., McDermott,P., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Nadendla,S.,
            George,J. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CA v. 8.2
            Genome Coverage                   :: 287.835x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 02:06:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,136
            CDSs (total)                      :: 5,014
            Genes (coding)                    :: 4,848
            CDSs (with protein)               :: 4,848
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 68 of 166
            Pseudo Genes (incomplete)         :: 111 of 166
            Pseudo Genes (internal stop)      :: 24 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            Pseudo Genes (short protein)      :: 1 of 166
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022065.2.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11366
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium var. 5-"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_312"
                     /serovar="Typhimurium var. 5-"
                     /isolation_source="Ground turkey"
                     /host="Environment"
                     /sub_species="enterica"
                     /culture_collection="FDA:FDAARGOS_312"
                     /db_xref="taxon:1620419"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: MD"
                     /collection_date="2011"
                     /collected_by="FDA CVM"
     gene            complement(477..989)
                     /gene="pilV"
                     /locus_tag="CEP90_RS27810"
     CDS             complement(477..989)
                     /gene="pilV"
                     /locus_tag="CEP90_RS27810"
                     /inference="COORDINATES: protein motif:HMM:NF016785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="WP_323741130.1"
                     /translation="MRSNNGWLITRNSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGG
                     QVKGGTVRADGRLYTGEYLQLERTAVAGASCSPNGLVGRDNTGAILSCQSGTWKSSSAS
                     IWTNIKTFTLYPKNTQVLGRFKLCINTYRIDGREMAETEVVPIDMPDSNGEMTWQAKNY
                     TQYSSYL"
                     /name="pilV"
     gene            complement(965..1246)
                     /gene="pilV"
                     /locus_tag="CEP90_RS27815"
     CDS             complement(965..1246)
                     /gene="pilV"
                     /locus_tag="CEP90_RS27815"
                     /inference="COORDINATES: protein motif:HMM:NF016785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="WP_267253664.1"
                     /translation="MLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGSNNLNNV
                     GAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVMSPLAVISEVIMVG"
                     /name="pilV"
     gene            complement(1293..1517)
                     /gene="pilV"
                     /locus_tag="CEP90_RS27820"
     CDS             complement(1293..1517)
                     /gene="pilV"
                     /locus_tag="CEP90_RS27820"
                     /inference="COORDINATES: protein motif:HMM:NF016785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="WP_267253665.1"
                     /translation="MSSGFTETTARGSGYRHMWFERPNPELLQAMVVSSGGTPYPVKAL
                     IQMAKDITAGLGGYIQDGKQPQVHYVPGQ"
                     /name="pilV"
     gene            complement(1776..2429)
                     /locus_tag="CEP90_RS27090"
                     /old_locus_tag="CEP90_27090"
                     /pseudo=""
     CDS             complement(1776..2429)
                     /locus_tag="CEP90_RS27090"
                     /old_locus_tag="CEP90_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /name="prepilin peptidase"
     gene            complement(2414..2875)
                     /locus_tag="CEP90_RS27095"
                     /old_locus_tag="CEP90_27095"
                     /pseudo=""
     CDS             complement(2414..2875)
                     /locus_tag="CEP90_RS27095"
                     /old_locus_tag="CEP90_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(2978..3574)
                     /locus_tag="CEP90_RS27505"
                     /pseudo=""
     CDS             complement(2978..3574)
                     /locus_tag="CEP90_RS27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /name="type 4 pilus major pilin"
     gene            complement(3605..4700)
                     /locus_tag="CEP90_RS27105"
                     /old_locus_tag="CEP90_27105"
                     /pseudo=""
     CDS             complement(3605..4700)
                     /locus_tag="CEP90_RS27105"
                     /old_locus_tag="CEP90_27105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /name="type II secretion system F family protein"
     gene            complement(4712..6263)
                     /locus_tag="CEP90_RS27110"
                     /old_locus_tag="CEP90_27110"
                     /pseudo=""
     CDS             complement(4712..6263)
                     /locus_tag="CEP90_RS27110"
                     /old_locus_tag="CEP90_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /name="ATPase, T2SS/T4P/T4SS family"
     gene            complement(6274..6725)
                     /gene="pilP"
                     /locus_tag="CEP90_RS27115"
                     /old_locus_tag="CEP90_27115"
                     /pseudo=""
     CDS             complement(6274..6725)
                     /gene="pilP"
                     /locus_tag="CEP90_RS27115"
                     /old_locus_tag="CEP90_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /GO_process="GO:0009297 - pilus assembly [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /name="pilP"
     gene            complement(6712..8004)
                     /gene="pilO2"
                     /locus_tag="CEP90_RS27120"
                     /old_locus_tag="CEP90_27595"
                     /pseudo=""
     CDS             complement(6712..8004)
                     /gene="pilO2"
                     /locus_tag="CEP90_RS27120"
                     /old_locus_tag="CEP90_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /name="pilO2"
     gene            complement(7997..9676)
                     /locus_tag="CEP90_RS27125"
                     /old_locus_tag="CEP90_27130"
                     /pseudo=""
     CDS             complement(7997..9676)
                     /locus_tag="CEP90_RS27125"
                     /old_locus_tag="CEP90_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0009297 - pilus assembly [Evidence IEA];
                     GO:0009306 - protein secretion [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /name="PilN family type IVB pilus formation outer membrane
                     protein"
     gene            complement(9690..10126)
                     /gene="pilM"
                     /locus_tag="CEP90_RS27130"
                     /old_locus_tag="CEP90_27135"
                     /pseudo=""
     CDS             complement(9690..10126)
                     /gene="pilM"
                     /locus_tag="CEP90_RS27130"
                     /old_locus_tag="CEP90_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /name="pilM"
     gene            complement(10126..11186)
                     /gene="pilL"
                     /locus_tag="CEP90_RS27135"
                     /old_locus_tag="CEP90_27140"
                     /pseudo=""
     CDS             complement(10126..11186)
                     /gene="pilL"
                     /locus_tag="CEP90_RS27135"
                     /old_locus_tag="CEP90_27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /name="pilL"
     gene            complement(11219..>11366)
                     /locus_tag="CEP90_RS27910"
                     /old_locus_tag="CEP90_27150"
                     /pseudo=""
     CDS             complement(11219..>11366)
                     /locus_tag="CEP90_RS27910"
                     /old_locus_tag="CEP90_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334619.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /name="pili assembly chaperone"
ORIGIN
        1 gcgtcccacc aacatatcct gcagtcggga tgtatttgca catgcacctc cggcagatcc
       61 tccattacct ccagatgcat aataaacaat gtactgcccc tgaattcccc tgagaatgaa
      121 cctctatgtg aactaagttt gtgtaagaac cattgagaac cagtcttcac gtaccggatt
      181 ggcactggcc tacgtgccaa tccggtacct ggagaaaggt aggatctggt gagcttcaaa
      241 ttgctacagc acaggccaca ggttggcgct ttcctggagc aacagctact gtcaactgga
      301 aaacgtgtta caggtgcggg cggaatatgc acctcacgaa ctggttacat atggcttacc
      361 agtcattccc atcagccaat aactcatggt cggctgcgtg cgatacaacc gaagatcaga
      421 atggttcaat aactgtatat gccatttgcc aataagctat ttcaagcaag taatcttcat
      481 aagtaggacg aatactgtgt gtagtttttg gcttgccaag tcatctcgcc gttagagtca
      541 ggcatgtcaa taggaacgac ttctgtttct gccatttcac gcccgtcaat acgataggtg
      601 ttaatacaca gtttaaacct ccctaagacc tgtgtgtttt tgggatataa cgtaaatgtt
      661 tttatattcg tccatatcga cgctgatgat gatttccagg taccggattg gcacgaaagt
      721 attgcccctg tattatcgcg gcctacaagg ccgttaggcg aacatgatgc gccagcaacg
      781 gcagttcttt ccagttgtaa gtattcacca gtataaaggc gaccatcagc ccgaacagta
      841 ccgcctttca cctgaccgcc ggtatagatg cccttgttgt ttacacttcg aacccatgat
      901 ccatcggaca tataaaatcc gccaccgtga gtttcattga gccagccttt actgttacga
      961 gtaattaacc aaccattatt acttctgata tcaccgccag cggtgacatt acgtcaaagt
     1021 ttccgctatt gccttttacc tgaccgctga aagtgccatt tacaccattc acattgccgc
     1081 tgaaattacc tgtctgggca tttactgccc caacgttatt caggttattt gatcccatat
     1141 caatggccgt gtgcattttg tttaagtcag ggcgaccatt gacctggaat ctgtaaagac
     1201 gatcagtgtc ctcagctgca ccactaagtt catctgtcga taacaatacg gcaatatgcc
     1261 cgttaccgct ttggcaccat aattacttaa agctactgac caggaacgta atgcacctgt
     1321 ggctgtttgc cgtcctggat atatccacca agaccagcgg taatatcctt agccatctgg
     1381 ataagtgctt tcactggata aggtgtgcca ccactggata caaccattgc ctgtagtaat
     1441 tccgggtttg ggcgttcgaa ccacatatgc ctgtaaccgc tgcccctcgc tgttgtctca
     1501 gtaaacccgc tggacaaaaa gccggtattt tcagcatggt tgtcgtgata acggcaggag
     1561 ttgttgtggt actactgccc tggagagtcg tgtagttttt ccctatataa gagcgggccg
     1621 cactggtcca gttgctgaca agacgtgctt cagtttgcca tccttttgtt tgaatatagt
     1681 cctgccatat gcccgctccc caggcaatca ggagcataac aatcagtaaa gccgcgccag
     1741 tttccagcga tgcccagcct cggtcatatt tttcatcaga ttacccatag tggttgataa
     1801 agattatcca gcagcaaaag gacatggcca aaacatagcc atggacccag agggccttct
     1861 tatgcccttt tatgcaccat gttgaatgcc ataagacaaa tcctcctgtg cccagcaaca
     1921 cacaccatat ggcgttatat aaccctgacc atgcggtaat acctgcaata agccacaggt
     1981 ctccagtgcc aatattaaga cgatggcggt taactaattt atgaaggcag aataagacaa
     2041 ttgcagcagt ggcaaattct gttgtacgga accaccatat atcagtcgtg atttgtgata
     2101 gcattcctgc aattaaaaac gtcgggtgaa tgttcctggt aataaacctg tcagtgcatc
     2161 cgttaccgcc atttgcagta tgaacgaaag aataaggaca gcatgaatac ggtagagcag
     2221 tggtgatgat gataaaataa ttaacgccac agctatactg tatagccata ttcctgacag
     2281 aacagcaggg tgtgtcttat accagagatg tcccgtatca gataaaaata acgaactctg
     2341 aatagcagag ttcgtccaat aatggaaaat gtgaacatca atggcagtat ataaacaggg
     2401 gatagactta tgcctacgat tgtctgcatt gcctgcctcc ctgtccggga agacatatcc
     2461 ctttcatgtc acgataaatt ctccatattt attggcatat tgctctcttg tttcatgtct
     2521 gtccttacgg aatccggcat tgtatgaccc aaggcaattc cagctgacgc cacatatctg
     2581 aaaatgtctt gccagtatcc agcttcctat atggatatta aggcatggtt tggtaattaa
     2641 atcctcagac ttttttatta cgcccatatt tatcagcttt ggaatatggg ttgagttatt
     2701 tgcatcaacc cataatccgt gctactggct ttacctgttt tttgtcctta tttatattaa
     2761 ttgcgcccgg cctcaacgaa gattccccgg cagaaatagc cttcaataat aatggttcaa
     2821 tttgatagcg cgcgccggca gcggcaaagc aattgtccca cttgttggtc cagatctgtt
     2881 tgggagctac gtatgaagct cgggcgggtg gaataagaaa aaagcaggat taatgaaata
     2941 agtctgaggg tactgtaccc cctgacatta aaactcctta accattgatg gtgaagataa
     3001 gtttgtttgt accagtgctg ccattatctg ccttgcattg cgtactggct tcttcagtag
     3061 tgacttttcc atcactgtga gcagtactgt tcagggtaat gccgttagtt aaaccagttt
     3121 tactgattcg tgtggcaatc tggatacagg catcctgtgg gactttatca taggtacggt
     3181 aaaaccgttg ttgaaccctg atgttgaagc cgggcaacag ttacagcacc gccccatgcg
     3241 ttatagagcg ttgctgtacc agatgttttg tcgccctgta cggtcatccc agagggaatg
     3301 acacccatct gaatgagggc acctgtcatt ttggcactgc tggtaaatgt atatccatcg
     3361 ctacctttta gcaggctctg ggcacttgtg ataattgttt gtatatttgc ggtttcagtt
     3421 gccacattgg ctcttgtacg caacgcatac aaactaccca gaacaacgac tataacaaaa
     3481 gaacaacgag cgctattgtt ccctgctcca aaattgccca tcctttatca tgaggctcat
     3541 tcttcttatt atttccagtt aaagtggtat taatatttct actaacataa atatcctcgt
     3601 ttgtttaaaa gttacccatg ctgttgtcac taatagactg aatatccata atggcaaaaa
     3661 ccagtaacat gagagacatg acaagaaata tcagcataat taaacgaatc acgtttgccc
     3721 gtttctttac acgttgaagt gtttgactaa gccatcgttg accataattg ctgattagct
     3781 ctgttgcgcc atctccctgt aataaagaca ggaagttggc tgcttccctg gatgggaact
     3841 gataaccgca ctgacgcaaa gccagtccaa gatgatcccc ctggcggacg cggtaaataa
     3901 tgctgtcaag acgtgtgctt aaccatggtg atgcaaactc ctgaagaatg tttagcgagt
     3961 tgagcgtcgt cattttggct tcagaagtgc agccatattc aggagaaatg ttgctccctg
     4021 tatatcctga taaatactcc atggcattat cttgtcggca aaagtacgaa cactgtcagg
     4081 gcttttccag tttggcagag accatgatat taaacctgtg atcactgcaa aaagaacggc
     4141 acatatagcg ccgtaattat cgacaaaaac agataatcca taaagaaaac caagagcacc
     4201 actccatgaa tcgggggagc ttattttgct cagttcaggt attagctctg tattcagaac
     4261 atataatgtc cccgtcatca tgattaacaa tcccaccgga taaatagcca tttgccatat
     4321 agcctgatgg atttgttctt ttgcatctgt aagagtggtg gcgaattgca gtgcatccac
     4381 aattgaacca cttcttatcc cggcgctgat tactgcggcc tcttcctgag gaacccataa
     4441 actaagagta tattccagag agttttcgcc actgttttca cgaagagact caatgcagtc
     4501 tgtggccagt tcagcaaagg gatgccattt tcgtccaaag tctgtccagg catcccgcat
     4561 ctgttccagt gcggtcttta atggctgttt attttccagc agaaaacgca gggcttcgta
     4621 aaactgcacc ctgtaggggg cgctgaacgt ttttctgaca atgaacgcct cagccgttga
     4681 ctgaaattca tttcacgcat gttgtttcct gttagacatc atcgatgctg aggcgctcgt
     4741 cttcgtccag aggatgattc tgtcggcttc aagagggtct accagacctt cattgatacg
     4801 gcgaagaaga tgctctacac gactgatgcc tttcatgttt tccagccagt atttacgtgc
     4861 tgcaactttg ccgcgtgttt tcagaatctg aaataagcgg ttatcaggtt ctatcacctc
     4921 agcaattaca gtcctgccgg tgaggccttt acctatttca ccgcgctttc tgccgttaat
     4981 gattacgtcg tgattacatt cagagcatcc gtgatgattt cggaaccaga tattatctgt
     5041 actgcagagt gaatctttat tgcagtgccg ctcaaggtaa tcacgctcgt cgtcgctaag
     5101 ttcaggtgct cgcttttccc atggaatacg gcaggaggga cacagagttg gtacaaggcg
     5161 ctggctgatc atcccaatca gtaattgcgc atcgcaaata agatcggcat tcatacccat
     5221 cgtaatcatt cgctccggta tacccagagc tgagtttgta tgtaatgtcg tcagaactat
     5281 gtgcccggtc tgtgccgcgt aggtggttga catcatcgag ataagatcgc gcatttcccc
     5341 ttccatgatg gcatcagggt caagtcgcat tgccgatgaa atagcccgac tccatgccag
     5401 tttgacggca tcttcgtcgg atttgtcgca gataatcgga gtctgtgttg ctcccagtat
     5461 ctgtccttcc agcggatctt cgattgttag caggtgtcgc ccctgattat cgtcaagata
     5521 tacacggcag gcgctgcgta acgttgtcga tttaccggaa ccggttggac ctgacaggac
     5581 tatttttcct tccgggcggc gcagcattat gttcagcaac cggatttgtt ccgggataaa
     5641 gcccagttgt ttaaacgtgg gaactttatc tccgtcatcg ggtatcaggc gcataactgc
     5701 aatcagaccg tctcccgtag gtctgtgact gtagcgtgca ccaaatattc ctatttttcg
     5761 cattaattgt ggggacaggc gcgcatcctg ctccctttga gggaagaaac tggtttcagt
     5821 gacatcagcc attgacagaa tggctgttgc gcaaagtgaa tagcccagtg ctggttgatc
     5881 ttcatctaca gtctgcagtt caccgaatat tctcatgcga actttgaaaa tagactctga
     5941 aattaagaaa tgaatatctg aagcaccaag ttttctggca gtttcaaaat aagatagaac
     6001 tttcttctca ctttcactga catcagcaag gtctttcaga tgaataattc gttctttcgg
     6061 gcctctgttt ttctcatcct gacctgcctc ctgtaactcc gacaacgaga ctacttttgg
     6121 gttgatatcc ggatataatt ttagcagctc ctggagatta ctttgtacgt caggccttgt
     6181 ccgctggttt gtttctataa gaatttcatg ggtgtctccc ctggatataa aaggagagca
     6241 tccttcagat tatatttatc catatcacgt tactcagaaa gtaagggtgg tgccatcact
     6301 taacgtgacg ccagacaatg agatcgactt cactgtgacg gatgtgcctg gaagctggct
     6361 gccagtggtg acgcttgtct gacgtccatc agccatgcga agaacggcat taagacgttt
     6421 atctttgccg tttatttcca taataacggg caattccttt ctgttttaga tgtcggctgc
     6481 tctgaaactg atggcaaaga ggcagtcaca ccagagaaac ctgaaacagt ttcagatgat
     6541 gaagtaacat ctgattcttc gagttgtttc tgtagctgtg cgccctgaac ttttgcctgt
     6601 aaaagaatat tccggttttg ttgtgcttca agttctccta ttgtgacaag aggttgtgtt
     6661 gttgcaaatg aaaaaccgga gaagaaaaat atggatggaa taatgagtaa tttacctgga
     6721 cgcataaata tgaccttctg aggaataact gaaagtaccg ccgtttaatt caaaatggat
     6781 attgctcaga cgaacgccgg tatcctgaaa taaagggaac aactcatcag gatttacagg
     6841 cgttgataaa ctgaactgat attctttcca tttctgaaca gggctggttc accatcgtta
     6901 cctggtaatg gttccggtat ggctatttca ttaatggccg gcgttaactg ttttttctga
     6961 aaccacgtga acacccgcat cagttgttct gagggggtag ggactgcctc atccctgcgg
     7021 ggcagggaag gcagtggccg ggttacagaa gccaggcgtg caccatcttt aaggttaaag
     7081 tcaggaataa cattaaagat ttctttgctt cttgccagaa agccttcaat agttcctcct
     7141 ggctgtcgtt cgtaaatcag tgtgaatgtc tcgggagtac atgttccacc ggtaagtttc
     7201 cagccttcta gcgcgaccgg tgatggtttt cgaaggtccg cgcaggcttt cagaaaatca
     7261 ctgattacag gttgtgatgc ccatggatgc gggagttctg gcggtggatc gggcttttta
     7321 aactgcaatc ttgcccgtgc agctatctcc tcgggcgtcg gtacgacatc cggctctggc
     7381 tgagtcatcc agaatacagt gcctgcgctg gctgctacta aaaaagtacg gcaggcagtg
     7441 tgaatttgct gcgattgccg acagtaagtt tgcaacggcg gagatctgct gatgataacc
     7501 gggtaataat actttcccag ttatcaggat gttccagagg agatacgacc tgccattttt
     7561 cggggggctc ttcgttcatc gtcagaaaaa gactgacctt ttgagcaaca tcagcatcat
     7621 ttcctgacag atcagccata acggcaggct ggccattaat ggaggcaagg aataaaagct
     7681 ccttatcgct gtaccggaag acgccgtatc catttctcac ccatgagcag aaagccagag
     7741 caagggaaaa taatgattgc gctccggcct ggccagtatt cctgtgccgg taatgcggct
     7801 gatcttccca cgggaacttc tgctgcctac agtcactttc agagaggtaa gccgtctgga
     7861 ttttacatgg ggcttgtgtt tctgatgaga agggactcgc tgttttttta cgacaggact
     7921 ccatttaaga caggctgccc acacgcgatg atttacttta ggatctgcga gaagtacaac
     7981 cgggttgata tcttcatcag ccattgttgc ctcctgacgg gaaagtcggg gtaatgatga
     8041 ttacaagcgt gctgcgttca tttttcccgg tttgtgaacc accaaataat ggatttgctg
     8101 gcgtaaacgt accggcttta cttgtcgtcg tattgttctg atcgaaacca gtaacgacaa
     8161 gtgattgccc ttctttcaga ttgaccttct ggctcagtga acgcagtttg gtatacggca
     8221 tttcgatgta actgtttccg tcttttgacg taaagctacg gattgttggg gatctgacag
     8281 attaaaattc atctgcaact ggagattgcc cgttttctga attaaaggca gcagggtcat
     8341 attgaatccg gtggtgatca tgcccggcgt taatgttgtt gttgctccta catccgtagt
     8401 cgttgttgtt gctgactggg cgacgtaaac cgtctgatct gccatctgga taggtaccgg
     8461 cgtcaggttt gtgacagtgc tttcttgtga agtcacaaca ctgacatcgc cctgttcact
     8521 cagcgcttta atcagaagac tggaaccgct gaattggcgg cattccctgt tgccgtatcc
     8581 agaattgata cgcctgcaga tgtagcgcct gtaaaatctc cgcttgcatt gttcaacgtt
     8641 gctccggcgg aatgtagcga tttataaaca aggttccagt ccagaccgaa ctgttcgttt
     8701 ctggtattgc tgacgctcag aacctgtacg ttcagggcta cctggcggtt cataatactg
     8761 ttttgttcgt ccacatatcg tgcgacggct tcctggacag ctggagtatc agtgacagtc
     8821 agcgttgagc tcgatgcaga taaccagtaa cggccttttt ctggtgtcag cattgcttca
     8881 atagttttcc ggatgtcttc atacagatcg tattcctgac caacggttgt gctctgagag
     8941 gacgttgcat caccggatgc tgagttatcc tggcctcctg ttgcccccat tgtgcttgtt
     9001 gagccagagc tgacactgga actgctgctg gttttggtgt tcagcatatg aagtggatac
     9061 gttctggttt cagtcagata gaatacaatc cgaccattat ccatgcgcca gtacagacca
     9121 ctccggctgg ccatcagatc cagaagacca ttgatatctc cctgccacat gaggttattc
     9181 agcgttaatg gctgagtgga ggtagtcatt gttgtgctgc ccaggctgct taacggtaga
     9241 cgcccatttt catctggtgc tggtagtgtt cctgtcatct ggcgggtagc gcctccttca
     9301 agagttgaat tggctgcgtc aggcgtgatg atcacaggga tgccgcatac ggcagtaata
     9361 cgttgcccca gttcctgcag agtgatctct cctttcttgc ctgcgtgatg tagcaggccg
     9421 gagctgtttg ttttttctct cttgatacct gagcgaccgg aacagggttt atccattgat
     9481 tatcgagcca ggtaagagcc tgcgatttac gggccgaaag ggctgatacc ttttcccgtg
     9541 catgtgctga gtcttcctgt gcttttttct gcattttgtt gatttcgctg aaagtacatc
     9601 cggagatgga aagagcgacg gcgatcatgc aggggagcac cgccagcttc attgaacgct
     9661 ggtgtgattt tttcatgaat atatccagat tagttaacgt agacgacaga accagcagta
     9721 atgcctgccg ggggtgtcag accggttgca gttcctgata gccaggtgag ttgcccgttt
     9781 tggaagatcc ctattagtgc agaaccacga ctttttgaac gcaaagtttc aacaagacct
     9841 ggttgttcag gcatccatac ccacagacgc ccctgttgta actgatgttt aattcgggag
     9901 tccggagtga cagggagagc cagtttatct gaggaaatta ctccgtcctg ctggcctgat
     9961 acgtatctga tatcattgat tctgttagca atcatcagta tctgacttgc ccatacagac
    10021 gcacttgcat attgctgggc gttgtggttt cagacatctt ctgattctgg tagctaccag
    10081 tgataatcag aatggtcagg ccgacggcca tgaccagcca tcccattagc gaccatccct
    10141 tgtatcagaa actgtaatga tgcactgcat gcgagaagcc tgtgcgtata aaggtttttg
    10201 cgcagtccga tataactcaa atacctgctc catggctgat tcaaatgatc cccggaacat
    10261 taaaggcgca tccagacggt aatcggttac agaaagtggc catatgacca tccagtgcgt
    10321 tgatgccatg ctttcgcatt ttgtttcttc tgcccacttg ataatgttct ctcttaatgt
    10381 ggtgcctgcc ggcgcacgcc actctttacc ctgagatacc ggtttaacag gcgttccggt
    10441 catgagcggg atagacttga ctgtggagcc ggttggagtc ggggtggcgg cgggggttga
    10501 cgaagatacg ctgttcctct gaatggattt cgtggttgtt ctggctattt gctgttgttg
    10561 gagattcagg ggaagtggat gtggttacca tggctgctgg cgagatagtg gttgccgtgt
    10621 cctgtgccgg tgcagcagct gatgttgtga cggtcaacgt ttattatccc agtccagatg
    10681 gccccatagg tgttgttctt ccagcaaccg attcagagaa cgggtccact gatcattggc
    10741 agaccatgaa accagtttgg tattcagctt gggcgtggct gcattctctt gtctgaattt
    10801 ccatccttcc ggtagtaatg ttttatccac tgactgacgg tccggttacg ggccgtcggc
    10861 gatagtgtta caggggcgct accaggcttg cctgtaatga ttaaccggga gaaattctga
    10921 cttattgttg gtgaaggtaa cggtcgggat tcgctgactg gatgtgctgt ttttactgtc
    10981 tctggcacgc tgactctcgg gggggagtta ttttaggtgg agaaagacgt tgttgcgtaa
    11041 gtgccagaac gccagcactc tcactgattt gtccgtcaac aaaaccagag caggcgtatc
    11101 tgatggtgcc acgtattgct gccggaaatt ccggcacagc cggacaataa tgacggcagt
    11161 ataagtgtca gatagttttt tttcataacg gagactccgt gttcgtatta cgtgtttgtt
    11221 aattggtgtg aggcggctgg agaggacgaa tgacgactgt cattccgtcc gaatgcaaat
    11281 gcgggagaga ccactgaaat aagagatata caaaaggaat tgataatctt tcatacagac
    11341 ttgttttccg tgggcatgta ataaca
//
