LOCUS       NZ_CP022060            11782 bp    DNA     linear   CON 07-JAN-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Typhimurium var. 5-
            strain FDAARGOS_312 plasmid unnamed1.
ACCESSION   NZ_CP022060
VERSION     NZ_CP022060.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06173325
            Assembly: GCF_002208965.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium var. 5-
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium var. 5-
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11782)
  AUTHORS   Zhao,S., McDermott,P., Tallon,L.J., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Vyas,G., Nadendla,S.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11782)
  AUTHORS   Zhao,S., McDermott,P., Tallon,L.J., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Vyas,G., Nadendla,S.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 11782)
  AUTHORS   Zhao,S., McDermott,P., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Nadendla,S.,
            George,J. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CA v. 8.2
            Genome Coverage                   :: 287.835x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 02:06:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,136
            CDSs (total)                      :: 5,014
            Genes (coding)                    :: 4,848
            CDSs (with protein)               :: 4,848
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 68 of 166
            Pseudo Genes (incomplete)         :: 111 of 166
            Pseudo Genes (internal stop)      :: 24 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            Pseudo Genes (short protein)      :: 1 of 166
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022060.2.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11782
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium var. 5-"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_312"
                     /serovar="Typhimurium var. 5-"
                     /isolation_source="Ground turkey"
                     /host="Environment"
                     /sub_species="enterica"
                     /culture_collection="FDA:FDAARGOS_312"
                     /db_xref="taxon:1620419"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: MD"
                     /collection_date="2011"
                     /collected_by="FDA CVM"
     gene            complement(<1..418)
                     /locus_tag="CEP90_RS00005"
                     /old_locus_tag="CEP90_00005"
                     /pseudo=""
     CDS             complement(<1..418)
                     /locus_tag="CEP90_RS00005"
                     /old_locus_tag="CEP90_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /name="DUF5710 domain-containing protein"
     gene            complement(508..1059)
                     /locus_tag="CEP90_RS00010"
                     /old_locus_tag="CEP90_00010"
     CDS             complement(508..1059)
                     /locus_tag="CEP90_RS00010"
                     /old_locus_tag="CEP90_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="WP_000014584.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGSA
                     SALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQEAM
                     KYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNMPDV
                     AAQYLEHWQDRWNKGTDWRP"
                     /name="phospholipase D family protein"
     gene            complement(1074..1364)
                     /locus_tag="CEP90_RS00015"
                     /old_locus_tag="CEP90_00015"
     CDS             complement(1074..1364)
                     /locus_tag="CEP90_RS00015"
                     /old_locus_tag="CEP90_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001299214.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTGI
                     ILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
                     /name="hypothetical protein"
     gene            complement(1361..2508)
                     /gene="traJ"
                     /locus_tag="CEP90_RS00020"
                     /old_locus_tag="CEP90_00020"
                     /pseudo=""
     CDS             complement(1361..2508)
                     /gene="traJ"
                     /locus_tag="CEP90_RS00020"
                     /old_locus_tag="CEP90_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /name="traJ"
     gene            complement(2505..3323)
                     /gene="traI"
                     /locus_tag="CEP90_RS00025"
                     /old_locus_tag="CEP90_00025"
     CDS             complement(2505..3323)
                     /gene="traI"
                     /locus_tag="CEP90_RS00025"
                     /old_locus_tag="CEP90_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="WP_000646097.1"
                     /translation="MKALTSLILPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSEK
                     PDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEGWL
                     PPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGTEGS
                     VVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQGMITR
                     PDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
                     /name="traI"
     gene            complement(3320..3778)
                     /gene="traH"
                     /locus_tag="CEP90_RS00030"
                     /old_locus_tag="CEP90_00030"
     CDS             complement(3320..3778)
                     /gene="traH"
                     /locus_tag="CEP90_RS00030"
                     /old_locus_tag="CEP90_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="WP_001079808.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQY
                     TLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVRLP
                     LPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
                     /name="traH"
     gene            complement(4173..4757)
                     /locus_tag="CEP90_RS00035"
                     /old_locus_tag="CEP90_00035"
     CDS             complement(4173..4757)
                     /locus_tag="CEP90_RS00035"
                     /old_locus_tag="CEP90_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="WP_000977517.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEINA
                     DNTVIFLFRHGEHCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNAVR
                     TIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLARDRL
                     GKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
                     /name="histidine phosphatase family protein"
     gene            complement(4816..6032)
                     /locus_tag="CEP90_RS00040"
                     /old_locus_tag="CEP90_00040"
                     /pseudo=""
     CDS             complement(4816..6032)
                     /locus_tag="CEP90_RS00040"
                     /old_locus_tag="CEP90_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /name="conjugal transfer protein TraF"
     gene            complement(6102..6925)
                     /gene="traE"
                     /locus_tag="CEP90_RS00045"
                     /old_locus_tag="CEP90_00045"
                     /pseudo=""
     CDS             complement(6102..6925)
                     /gene="traE"
                     /locus_tag="CEP90_RS00045"
                     /old_locus_tag="CEP90_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /name="traE"
     gene            complement(7076..8230)
                     /locus_tag="CEP90_RS00050"
                     /old_locus_tag="CEP90_00050"
     CDS             complement(7076..8230)
                     /locus_tag="CEP90_RS00050"
                     /old_locus_tag="CEP90_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_001139958.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPIA
                     LRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTCRT
                     NPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEILALR
                     WEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKNAWRI
                     ATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRAWQLVS
                     KLDARRRQTQKVAAWFVPYPAHITTINEENGQKAHRIEIGDFDNLHVTATTKEEAVHRA
                     SEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
                     /name="site-specific integrase"
     gene            8921..9169
                     /locus_tag="CEP90_RS00060"
                     /old_locus_tag="CEP90_00060"
     CDS             8921..9169
                     /locus_tag="CEP90_RS00060"
                     /old_locus_tag="CEP90_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001349157.1"
                     /translation="MFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGKT
                     AFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
                     /name="hypothetical protein"
     gene            complement(9166..10458)
                     /locus_tag="CEP90_RS00065"
                     /old_locus_tag="CEP90_00065"
     CDS             complement(9166..10458)
                     /locus_tag="CEP90_RS00065"
                     /old_locus_tag="CEP90_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="WP_032274560.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEAR
                     LVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRLQA
                     YVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITAGLGGYIQDGKTATGALRSWSV
                     ALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGSNNLN
                     NVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITRNSKGW
                     LNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERTAVAGAS
                     CSPNGLVGRDNTGAILSCQSGTWGTIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAH
                     YCQVVESPAGSRKWYKYEHKTGCIASCVTLN"
                     /name="shufflon system plasmid conjugative transfer pilus
                     tip adhesin PilV"
     gene            complement(10458..11114)
                     /locus_tag="CEP90_RS00070"
                     /old_locus_tag="CEP90_00070"
     CDS             complement(10458..11114)
                     /locus_tag="CEP90_RS00070"
                     /old_locus_tag="CEP90_00070"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="WP_001193553.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWYK
                     THPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFTRR
                     FLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITAWSG
                     LYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI"
                     /name="prepilin peptidase"
     gene            complement(11099..11656)
                     /locus_tag="CEP90_RS00075"
                     /old_locus_tag="CEP90_00075"
                     /pseudo=""
     CDS             complement(11099..11656)
                     /locus_tag="CEP90_RS00075"
                     /old_locus_tag="CEP90_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(11666..>11782)
                     /locus_tag="CEP90_RS27835"
                     /pseudo=""
     CDS             complement(11666..>11782)
                     /locus_tag="CEP90_RS27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /name="pilus assembly protein PilX"
     Typing          complement(2509..3323)
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.16e-178"
ORIGIN
        1 gaaggtccag gtgatcggcg aattatccgc gctgcgataa tccctgtacc agccggcagg
       61 tctgccgtcc aggaatccgc gatatgcgcc acttttttgt ccttttttgt catcggcggt
      121 cgggacacgg tggattttcc cgtccatgac cggcagttct ttcagaacca gtccggcatt
      181 ctccagaacc tgggcaaact cggtgaccgg atcactgccg ttcatggaga cgtcctgtgg
      241 ccggggcagc cagcggtcca gcctggacag gtccgccccg ggcgggcgaa ccagagtttg
      301 tgctccttgt cccagatgac gggcgtgtgc ccgttcatcg gggatgtgct tttttgagat
      361 cttctatttc atctgcaggc acggcaagcc aggtgcggta gcgggataca gacggcatga
      421 gtgcttctcc tgaaaaaaga cggaaaaaaa ccaccgccgg aagccggggg tggctggggg
      481 gatagaaaat cgggggagaa atgtttgtca gggtctccag tctgtaccct tattccagcg
      541 atcctgccag tgttcaagat attgtgcggc tacatccggc atattcttga gcacaacgac
      601 attctctgaa ttcttgcgtg cagcagcacg agagaagtta taggagccag tttcaaccgt
      661 gtttccgtca acaatgatga ctttgtcgtg atggatgggg aaggcatcta cagtccggag
      721 aggaatgtcc agcaggttga tatatttcat cgcttcctga cttgcccggt tcgtattccc
      781 cttgtcatca acaacaatac ggacgtccac tccgcgtttt ttcgcttttg ccagcgcatg
      841 cattacatcc gggtcggtga aggaatatgc catcatcctg atggagcttt ccgcactgtt
      901 gattgtgtca agcaccagag cggatgcgct tccttcagga gaaaagccga ccttcacgga
      961 tggcgtgtcg atcattgcaa acgatggtgt cgtgaacagt aacgggcatg ccagtagcga
     1021 tacccggagc agagtaacga tagttttttt acctgacata aagactccag aatttaatcg
     1081 aggccagtcc agtctcgtgg tgattccgta tggtggcgat accaccatgg acgaccggag
     1141 gcgatggccc ctctcagaat gctgaaaatg cgagtacaga tcgtcctgac agtccagcct
     1201 gaacggtcga gaataacgaa aaataaaatg atgccggtgc aaatccagaa ggttgttttg
     1261 gagggaaagg ggcatataaa cagataaatc atggtgaacg gcagaggtac gccctggatg
     1321 ttgactggcg gtgttgcata tcgccagaaa ccttctcttt tcatgaccgt aactcccctg
     1381 gcgggataaa aagaatcgct tctcttcggt caacttcatt ccggatatac atttccagaa
     1441 tctggtcccg gatacggcgc ttctcctgcc ggattatggc atcaatgtga tgcccccact
     1501 ctgcatgcgg cattccggaa agattgtcgc gaagctcatc gttaatgacg atatattccc
     1561 ggacggcttt acgaccaccg tcatttgttc tgaccagaac ctgaaccaga atgaactgca
     1621 acagtgacag gacagcccag gccatcgaat ccctgatgac agggggaaac agcccaagaa
     1681 gacgtgccag cgtttcacca ggagatttcg tatgcatggt gctcaggcag taatgcccgg
     1741 tgttgcctgc ctgtaccgct gcgtcggcgg tttcgttatc cctgatctca ccaacaccga
     1801 taatttcggg attacgtcgt acagcagaac gcagtccggc agcaaaactg accacatcac
     1861 gcccgatttc tgcctgatgc ggtgggagca gatcgttttc atttcccagg atatattcca
     1921 ccgggtcttc atatgtcacg attttggcgt cggggaaatt gtccataatg taacggtaca
     1981 gtgctgaaca cagcgttgat ttccctgacc cggtttcacc acatacaaat cccagaccgc
     2041 ttttgcagac cattgcttca agcaggtctg gttcaatgtt catggagagg atgtccggta
     2101 tttctgtcgg gatgacacgc atggttattg atattgcttt ctcctccgca ccagatgtgc
     2161 cctgaatgag gtgagagcgt aaccggatgc gttctccgcg tttaagacca tagcgaccgg
     2221 atgcatcccc gttaatctgt atcgtcctgt caaccggatt gcctgccaga acgcgggaat
     2281 gacttcccga ccgaacagtt catcaatcag ggaggacatg agtgtggttg gtaacgttgc
     2341 actggaacaa cgcacccgtc ggccaaagcg actgacagat acaggtgacc cgccggtcag
     2401 atccacatca ctgactttat gtgcggcaca ccagacaaag aaatgccgta gttcatctgc
     2461 cgtgaaacgc tgaaacggaa aaagaccaaa ctcatcagga atattcatcg ggagcctcct
     2521 tcagtgccta taagcggacg ccagtgagat ttctgaagat tgaattccgc atgattttta
     2581 aggcgacgaa cacgatctcc agtgaccagt tttttcccgt cacctgtaac ggtttgcatt
     2641 tgatcagaaa catccggacg ggtgatcatg ccctgacgcc agagaagtga gtacagcatc
     2701 atgccccggt aatcacgggt gagggttttc tggttggctt caagcgtcag gtcggcgttt
     2761 tgtcttcctt cctgccatcc ctgccgtaat gcggtttccc acacttttcg ttgcacgctg
     2821 tcttctggaa cgacgctgcc ttctgtaccc ggagtggctg ttgttgaaag cccccggaga
     2881 agccagtcac gccagcccgg tggattgctg acaaagcgtt cagggcgaat gatggtccag
     2941 acccgggaag aggtccggat ttgatctggc gtgatatgag ctacgtcctg tgcttcatca
     3001 ataaccggtg gtagccagcc ttccgggcta atcagtggcc ggaaatcgta taaggcatta
     3061 agtgtgcttt cccgggcgtt gagtgcctga atcagctccc atgaacgctg agccttgcca
     3121 ccacgaaatc caagcgtttt acctgcgtcg gtcagcattt tccagcgtgt ttctgacaga
     3181 ctatccggct tttcacttcg tggtttaagc catgcattaa tgtcaggtgg tggcgtattg
     3241 acaccacctg gcgagatggt aggggaagaa gaacaaccag taagcagtat tactggcaaa
     3301 atgagagagg tgagtgcttt cattttttcc ggttctccgg ctgcatgaag ctgacctgta
     3361 acaagcccgg atattgatgg attgttgctc tccaggctgt ctgggcttca ataagtctca
     3421 acgtattgga aaacgtcata tcccggacgt gaaggttgac tggtaacggc aggcggacac
     3481 cattgtagtt aaactgcatc cccctgactc tggccagttc gttcagtaat tcaattgcat
     3541 caccttccca gtcaaaggaa aggcgatcgc tgttagctgt cagaagatga gaccccgttg
     3601 aaaccggaag ttggccggaa aacacacctt tgtcccataa tgtgtactga gtgtgccggg
     3661 taaccgccac atcagaggca gcaccgtagt tacgcggaac aggagtatgc tgagaagggg
     3721 agttgcagcc agccagaaaa agggcggcaa gacaccccgc aggtataata cggttcataa
     3781 taattcctga gcagttaaat cagaatggga tataccggtg ctccggtaca gacaggatga
     3841 tgtttgtttc cggctatact tgcgttaccg gaaataaatg ccagtgtggc caggtaaata
     3901 gcctcatagt ttactgaagg tgattattcc tgatactggc tggttgatgt gtcgtcagac
     3961 ataaaacgaa tatgagcgta atctttaacg ctccgcgccg aatcgatcca tgatggatga
     4021 ctcgacgatt tatgaatcga acgtaccggc gcttgtccct gaaacagttc ccggccctcg
     4081 tccggataac ctgcaattca gacaggcaca tgtgcccgta cactcttgta tcttactgag
     4141 tatatcagaa tttatgtaaa ttcaaatatc ctctatgcct ctttgttgta ttttccgtca
     4201 agtatgagcc gggtaccatc gtaatgcatg atcagggcat caaggtacgc cggtttgaat
     4261 ttcttgccga gcctatctct ggccagaaat gataaacaat gattatgtgt cattattact
     4321 atgttccttt tatgagattc tcttgcgatt gattcaagcg tcttataaag atcattattg
     4381 cagtctgata aactgtccat aacgacgggt tctttaccgg agaagaattt tgcagtttgt
     4441 atggttctta ccgcattgct ggagtaaata tcatattcac tgaagattgt tgcgaactta
     4501 ataccttcct gctgtgcttt ctctgtaccg gtaatagtga ttccgctttt atccgaatag
     4561 catggcatat ctgaacggtc gcaatgttct ccatggcgga aaaggaaaat aacggtgtta
     4621 tcagcattaa tttcttttgc ctgttccagg gtgatatcgg gaagcgagct ttttgctttt
     4681 gtcactgccg ctaccaggaa aacagaaaat aaaagaatga atgctgtttt tgaataagaa
     4741 gaacgtggtt tcatcatgtt atttaaactg aatataaaaa tacccccggg tttagtgccg
     4801 gggcggggac agtaatcaga aagtgaactg aagttgtgcc attgcaccgt aatcgtggtc
     4861 agttcctacc aggccggaaa catcgatatg tacgacgtca aatggagaga taccaacgcc
     4921 agcggtgaag gcgcttccgg agtttgatgc catgttctga cgataaccgg cacgcaattg
     4981 cgcccatttc catgcgttaa attcagcacc aacacttgca aactgacgtt tgctgtcaga
     5041 agtgaagcca ctggcaggag tcagatcaac gtcaagagca gtggtaaaca ggtcattatg
     5101 ccaggataca ccggcggttg cctgtggacg aactttgaag gtttctttaa agccattgac
     5161 gactttagta tcaatactgc gtggaatgat gttctgggca acaagaccga cagtccagtt
     5221 gtcattcagg tctgtataag caccaatatc ggcgttgaat ccgtttttcg tgttgtggta
     5281 cttgtcaccg tcaaaatcgt ctttgtcgta atcacgaacc gttacgttgt agttaaacag
     5341 gtccacacgc tgatattttg gtgtgacgcc aagagaccat ttttgtccgg ccgtttccag
     5401 ttctttggca aatgaaattc cgacgtcagt aattacagct gcacgcccga atgcgcgtga
     5461 ggtcagggca ttcttatcga cgtcggtaat tgttccattg gcaactttgt ccagataatc
     5521 caggtctgca tcgtttactt ttccgtttac gctgacagtt ccgtaagact tgaccataag
     5581 ggcaaacggc agagtgtcat ttgccatggc tgctacagct gatacaccta cctgagcatc
     5641 tgcattgata ttgcggaatt cctgcagacg atgtttaaga tttgctgctg cctgttgtac
     5701 gccatgctgg ttatcaacag cactatcaaa caaatcccag tcatctttaa catcatcagc
     5761 tttatttgat acatcatcag gatcagcaac ttgtgcccca acagacggca gaagaaggct
     5821 gaaatcatca ttgctgttgt gctttgtcag taaagccggg tttgccagag gtgccacacc
     5881 ataatgggaa gatgctaccc cggtgccccc attgcatcgt tacgtgcttc aaaatatgac
     5941 gtcgctgctg cagcgttaaa ggaaaataat acagcagatg caatgatggt gcgggtaata
     6001 tgattttctt catcatttaa tttcctgttt atcagaattt agacaaaaca acgctatgac
     6061 acggatgtgc cgtaattttt tactttggta ctatctggta tttaccaggg taataaagac
     6121 gtattaaata agctaacagg tagtatttgc cctgtaataa ggttcccggc aaggccaggt
     6181 ctcaacgtga cgcgtcgcaa agacattgtt gtattctgcc tggttgtata ttcagttaac
     6241 cattttcttt tttcatcgtt cagaattgtt ggtgataatg atagtgccgg atatattaca
     6301 gaaccattta tttctttttc tgtctcaata ttaccccgta atattctctt ttcgtgtttc
     6361 ctgcgaaggg gaaactatgc cagaaaataa cttcagtgtt gctgctatca ttaataagca
     6421 ttctgaaatt atcagcagta tctggccatt caccggacaa cgctatcgga tagttttcat
     6481 ggccattcag ccagtccgga ttgagattaa aacattcagc aagatgttcg agagcgtcag
     6541 ctgaaagata gtctgcaagt ctggccgggt cctgtataac cgtggagcgg atattccatg
     6601 gcgacagcaa tgttgccagt aatggcgcat ttatatcatg cgacagcatg atgtattcaa
     6661 ttctgcttat tatgttccct gcggtgttat ccgcaggcat aaacatatta tgaagagcat
     6721 cctcaagcag gattcgggtt aaatcagcgg tggatatggc taattgttcg ctcattatcc
     6781 ccagaacttc tcttacccct tgggggtaac ggacctgcag aggtgtcggg tttatctgac
     6841 gaccgttgat gagtttttcg ccgattgctt ttttgtccag cgagagcatc attcgggccg
     6901 caataccggt cgtattcagt ctcattgttc ctccgactga aaaagtcttt tcagctcatg
     6961 tggttattgt atcttcggtg ttttttctgg tgaaacgaat aatttcgatc atgcagatcg
     7021 aatgtgtttg gcgagttgtt gcaactcgcc tttctgtatg tgttttgaat gatgtttaca
     7081 gcggggtgct gcccgggttc agagggcaaa tcataatgta gtcagggtcg ttaacaggta
     7141 acgctccggg agatgggaca cgttcgcctt tctgtgctgc aatggccagt gtacgcaaca
     7201 aaacctcact ggcgcggtga actgcttcct ctttcgttgt ggcagtgacg tgaaggttat
     7261 caaaatcacc gatctcaata cgatgcgctt tctgcccatt ttcttcattg atggtcgtga
     7321 tatgggcagg atacggcaca aaccatgctg ccactttttg tgtctgccgc cggcgggcat
     7381 caagtttact gaccagttgc catgcacgaa gatgagtata ccgtttcagc atattcatgg
     7441 aacgatgtcc tgatattgca gcaatctcca ttacattcag gctacccagt tcgaagaagc
     7501 ggcttattgc ttcatgccgt agatcgtgaa aatgcaggtc ctcgatgcga agtcgttgtg
     7561 tggctattct ccaggcattt ttaaagccgg atgcggtgta atcaaaaaca ttgccgtgga
     7621 gattaacggg catcatctga agaaagttac gggcacgtct ggacagagga acatcccgtg
     7681 agtgaccgtt tttggtttca ggtaaatgag ccacaccgtg gcgcaaatca atgtgctccc
     7741 aacgtaaggc cagtatttcg ccctgccgca tggctgtctc aagggcaaga tggaaaatga
     7801 catacaacat cagatttttt tcgcggaaat agcgagaaag gcgacgttct tctgaagacg
     7861 ttagccggcg atctcgtccg gaggatactt tcggcttgcg aaccagttca accgggttag
     7921 tacgacaggt tccccattca acacgagcaa tattgaacag agatgacaga agggcgagtt
     7981 caagacgtac agtattacct gtaatgggtt tacccgttcg ggggttgatt tctgctaaac
     8041 gaacgtctct gtatgtagca atatcaacgg ttgttatttc gtccatattc cgaagggcaa
     8101 tgggatatcg cttgataaca ttgctccggt aaaactcctg ttgatgccct ttcttgtgaa
     8161 cagaaactgt tttcaggtac ttatccagtg cgcgtgacag ggacatttta cggatgcgtg
     8221 gagacggcat gctaacctca aaagaggcaa aaaataatgc caacaatcat tctctgcaag
     8281 cgtgccaatc cggtacctgg agaaaggtag gatctggtga gcttcaaatt gctacagcac
     8341 aggccacagg ttggcgcttt cctggagcaa cagctacttg tccaactgga aaacgtgtta
     8401 caggtggcgg cggaatatgc acctcacgaa ctggttacat atggcttacc aggtcattcc
     8461 catcagccaa taactcatgg tcggctgcgt gcgatacaac cgaagatcag aatggttcaa
     8521 taactgtata tgccatttgc caataagcta tttcaagcaa gtaatcttca taaagtagga
     8581 cgaatactgt gtgtagtttt tggcttgcca agtcatctcg ccgttagagt caggcatgtc
     8641 aataggaacg acttctgttt ctgccatttc acgcccgtca atacgatagg tgttaataca
     8701 cagtttaaac ctccctaaga cctgtgtgtt tttgggatat aacgtaaatg tttttatatt
     8761 cgtccatatc gacgctgatg atgatttcca ggtaccggat tggcacgtag gccagtgcca
     8821 atccggtacg tggaagactt ctggttcgct caatggttct tacacaaact taggttcaca
     8881 tagaggttca ttctcagggc ggaattcagg gggcagtaca ttgtttattt atgcatctgg
     8941 aggtaatgga ggatctgccg gaggtgcatg tgcaaataca tcccgactgc agggatatgt
     9001 tggtgggacg ctaattagcg tgaatgccag caataaccct gcatatggaa aaacagcctt
     9061 tatcagcttt gctgtacctg caggtacttc ctatcagata acatcctatc caacagaaaa
     9121 tacatcatgt ggtgccgggg tattttcagt atttggatat caaacttaat tgagcgttac
     9181 acacgacgca atacaacctg tcttatgctc gtatttgtac catttgcgtg aacctgctgg
     9241 gctctcaact acctggcagt agtgggcatc ctctgcgttg cccattctgg caatggcaca
     9301 gaagtcgaat tgccccagat aacctgtggt ggaaagctga gtaactttga gttttccacc
     9361 tattgtcccc cacgtaccgg attggcacga aagtattgcc cctgtattat cgcggcctac
     9421 aaggccgtta ggcgaacatg atgcgccagc aacggcagtt ctttccagtt gtaagtattc
     9481 accagtataa aggcgaccat cagcccgaac agtaccgcct ttcacctgac cgccggtata
     9541 gatgcccttg ttgtttacac ttcgaaccca tgatccatcg gacatataaa atccgccacc
     9601 gtgagtttca ttgagccagc ctttactgtt acgagtaatt aaccaaccat tattacttct
     9661 gatatcaccg ccagcggtga catttacgtc aaagtttccg ctattgcctt ttacctgacc
     9721 gctgaaagtg ccatttacac cattcacatt gccgctgaaa ttacctgtct gggcatttac
     9781 tgccccaacg ttattcaggt tatttgatcc catatcaatg gccgtgtgca ttttgtttaa
     9841 gtcagggcga ccattgacct ggaatctgta aagacgatca gtgtcctcag ctgcaccact
     9901 aagttcatct gtcgataaca atacggcaat atgcccgtta ccgcttttgg caccataatt
     9961 acttaaagct actgaccagg aacgtaatgc acctgtggct gttttgccgt cctggatata
    10021 tccaccaaga ccagcggtaa tatccttagc catctggata agtgctttca ctggataagg
    10081 tgtgccacca ctggatacaa ccattgcctg tagtaattcc gggttttggg cgtttcgaac
    10141 cacatatgcc tgtaaccgct gcccctcgct gtttgtctca gtaaacccgc tggacaaaaa
    10201 gccggtattt ttcagcatgg ttgtcgtgat aacggcagga gttgttgtgg tactactgcc
    10261 ctggagagtc gtgtagtttt tccctatata agagcgggcc gcactggtcc agttgctgac
    10321 aagacgtgct tcagtttgcc atccttttgt ttgaatatag tcctgccata tgcccgctcc
    10381 ccaggcaatc aggagcataa caatcagtaa agccgcgcca gtttccagcg atgcccagcc
    10441 tcggtcatat tttttcatca gattacccat agtggttgat aaagattatc cagcagcaaa
    10501 aggacatggc caaaacatag ccatggaccc agagggcctt ctttatgccc ttttatgcac
    10561 catgttgaat gccataagac aaatcctcct gtgcccagca acacacacca tatggcgtta
    10621 tataaccctg accatgcggt aatacctgca ataagccaca ggtctccagt gccaatatta
    10681 agacgatggc ggttaactaa tttatgaagg cagaataaga caattgcagc agtggcaaat
    10741 tctgttgtac ggaaccacca tatatcagtc gtgatttgtg atagcattcc tgcaattaaa
    10801 aaacgtcggg tgaatgttcc tggtaataaa cctgtcagtg catccgttac cgccatttgc
    10861 agtatgaacg aaagaataag gacagcatga atacggtaga gcagtggtga tgatgataaa
    10921 ataattaacg ccacagctat actgtatagc catattcctg acagaacagc agggtgtgtc
    10981 ttataccaga gatgtcccgt atcagataaa aaataacgaa ctctgaatag cagagttcgt
    11041 ccaataatgg aaaatgtgaa catcaatggc agtatataaa caggggatag acttatgcct
    11101 acgattgtct gcattgcctg cctccctgtc cgggaagaca tatccctttc atgtcacgat
    11161 aaattctcca tattttattg gcatattgct ctcttgtttc atgtctgtcc ttacggaatc
    11221 cggcattgta tgacccaagg caattccagc tgacgccaca tatctgaaaa tgtcttgcca
    11281 gtatccagct tcctatatgg atattaaggc atggtttggt aattaaatcc tcagactttt
    11341 tattacgccc atatttatca gctttggaat atgggttgag tttatttgca tcaacccata
    11401 atccgtgcta ctggctttac ctgttttttt gtccttattt atattaattg cgcccggcct
    11461 caacgaagat tccccggcag aaatagcctt catataatgg ttcaatttga tagcgcgcgc
    11521 cggcagcggc aaagcaattg tcccacttgt tggtccagat ctgtttggga gctacgtatg
    11581 aagctcgggc gggtggaata agagaaaaaa gcaggattaa tgaaataagt ctgaggggta
    11641 ctgtaccccc tgacattaaa actccttaac cattgatggt gaagataagt ttgtttgtac
    11701 cagtgctgcc attatctgcc ttgcattgcg tactggcttc ttcagtagtg acttttccat
    11761 cactgtgagc agtactgttc ag
//
