LOCUS       NZ_CP021785             4484 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Acinetobacter baumannii strain A85 plasmid pA85-1b, complete
            sequence.
ACCESSION   NZ_CP021785
VERSION     NZ_CP021785.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07125723
            Assembly: GCF_002210065.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Hamidian,M., Hawkey,J., Wick,R., Holt,K.E. and Hall,R.M.
  TITLE     Evolution of a clade of Acinetobacter baumannii global clone 1,
            lineage 1 via acquisition of carbapenem- and
            aminoglycoside-resistance genes and dispersion of ISAba1
  JOURNAL   Microb Genom 5 (1) (2019)
   PUBMED   30648939
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Hamidian,M., Wick,R.R., Hawkey,J., Holt,K.E. and Hall,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUN-2017) School of Life and Environmental Sciences,
            The University of Sydney, Cnr., Maze Cres., and Bultin Ave, Sydney,
            NSW 2006, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-JAN-2017
            Assembly Method                   :: Unicycler v. JAN-2017
            Assembly Name                     :: Acinetobacter A85
            Expected Final Version            :: yes
            Genome Coverage                   :: 369.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 08:03:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,032
            CDSs (total)                      :: 3,936
            Genes (coding)                    :: 3,873
            CDSs (with protein)               :: 3,873
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 35 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP021785.1.
            Bacteria and source DNA available from Ruth Hall, University of
            Sydney.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="A85"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pA85-1b"
                     /geo_loc_name="Australia:Sydney"
                     /lat_lon="33.8650 S 151.2094 E"
                     /collection_date="2003"
                     /collected_by="Ian Carter"
                     /genotype="global clone 1"
     gene            <25..309
                     /gene="thyA"
                     /locus_tag="CBI29_RS19710"
                     /old_locus_tag="CBI29_04101"
                     /pseudo=""
     CDS             <25..309
                     /gene="thyA"
                     /locus_tag="CBI29_RS19710"
                     /old_locus_tag="CBI29_04101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997638.1"
                     /GO_function="GO:0004799 - thymidylate synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /name="thyA"
     gene            406..918
                     /gene="dfrA44"
                     /locus_tag="CBI29_RS19715"
                     /old_locus_tag="CBI29_04102"
                     /gene_synonym="dfrA39"
     ARG             407..915
                     /name="dfrA44"
                     /gene="dfrA44"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA44"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1178..1594)
                     /locus_tag="CBI29_RS19720"
                     /old_locus_tag="CBI29_04103"
     CDS             complement(1178..1594)
                     /locus_tag="CBI29_RS19720"
                     /old_locus_tag="CBI29_04103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002061210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_057690043.1"
                     /translation="MNNEWLSEKITYISALKNPSDAQKLLLELAQIQYRTPEQEKKLNA
                     LIKAEKAIDRANKQKVVVRKLINAEKEAERKARTRHLIQLGALFEIANLDKRDPAELLG
                     VLLKTAEIDPNDMKWQIWKELGQETLNQRKKDKY"
                     /name="conjugal transfer protein TraD"
     gene            1873..2028
                     /locus_tag="CBI29_RS20570"
                     /old_locus_tag="CBI29_04104"
     CDS             1873..2028
                     /locus_tag="CBI29_RS20570"
                     /old_locus_tag="CBI29_04104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004846790.1"
                     /translation="MQLKQVLGKIKDITFGVIAAPLIIIIMMWASILDQRAFKKELDRK
                     KEQEKT"
                     /name="hypothetical protein"
     gene            2028..2957
                     /locus_tag="CBI29_RS19725"
                     /old_locus_tag="CBI29_04105"
     CDS             2028..2957
                     /locus_tag="CBI29_RS19725"
                     /old_locus_tag="CBI29_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_057690044.1"
                     /translation="MAIARLSVKVGKAGKAAPHAEYIDRDEEKKLKQEQAETDLEHSAY
                     GNMPKWAEHNPITFWEAADLYERKNGSTYREYEIALPREMNAEQRLELVEDFIQSEIGS
                     KYPYQFAIHNPKAMDGNDQPHVHLMFNERLQDGIERDPEQYFKRYNSKNPERGGAKKDN
                     TGKSYQERKTDIKDLRQRWADLCNSHLEKHQIDSRIDMRSYKEQGIEKEPEKKLLPSQA
                     KDPEIREALQQSRTAYKELERLDLGDPKKDLKELKDSPISDKEIKQGIESFKADFDSFK
                     QLALEQYKAQQKLEREQQKTMKFRGMSR"
                     /name="MobA/MobL family protein"
     gene            2954..3454
                     /locus_tag="CBI29_RS19730"
                     /old_locus_tag="CBI29_04106"
     CDS             2954..3454
                     /locus_tag="CBI29_RS19730"
                     /old_locus_tag="CBI29_04106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057690045.1"
                     /translation="MTPDEKKLYALMAVAEQQQNLVNQAVKELQITKEHIQSTIAEQTH
                     HTVSKSLNMGLQEGTAELIKTAKALGRLNNSIESARDQLSWKLITIVLGIFAILMLGII
                     WLFSVYIKPLGNIPQIESLRQQGIQFDIGSCKVDGESKPCVRVMKKQCGYGKNGDFCVI
                     DPK"
                     /name="hypothetical protein"
     Typing          2029..2954
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.793"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcaaaaaa ttaggggcat aaagttttta ggtgtccctt tcaatattgc cagctacgct
       61 ctgctcaccc atatgatcgc acaagtctgt gagcttgagg tcggtgattt tgtctggacg
      121 ggtggtgata cgcacctgta tgccaaccac tttgaacaag ccaaacttca gctgagtcgt
      181 gagcctttag gtctatgtca gttaaaactg aatcctgaaa ttaaagatct attcgatttc
      241 aaatttgaag atattgaaat tgtcggctat gaatcccatc cggcaattaa agcgcctgtc
      301 acggtttaat ttttcccaaa cccaagaaaa tcaaaatccc actttgctta aagtgggatt
      361 ttgcatttta ataggaacaa tttttacttg tttttgagtg tgggtatggc atttcaggat
      421 ttagaagtcg ttcatgtcgt tgcaatggat cagcagcgct gtattggtaa ggacaatgac
      481 ctgccttggc atatctcagc agatctaaaa cattttaagg aaatcaccca gggcggtgta
      541 attgtaatgg gacgtaagac ccttgaatcc atgggacgtg ccttgcctaa acgtgtcaac
      601 tgggtcatta cccgtgatac agactggtct tttgaaggta ctaaagtcgc acacacgatt
      661 gaagatgcct tgaaccaagc tgttgcagat gtaaaagcgt cagaaaaacc ggagtctatt
      721 tatattattg ggggtggtga aatcttcaaa cagacaatga gtattgctga ccgtctggaa
      781 ctgacccatg tcgaactgga tgtacaaggt catgccttct acccggaaat tcctgctgaa
      841 ttcaaaaaag ttttttccga acaacatatc gacgacaaaa ctgggattgc ttttgagttt
      901 gcaacttata gaaaatgaag aataattttt aattaaagat tcaattatgc atatactcct
      961 gagctaattt ttcttgacgt ttttgttctt cttctaaacg ttttttatat gcgattctgt
     1021 catcagaaga ttctttttta tattgattaa aagagatctt cccttcaaca aggtcatgta
     1081 aagtattcag attattacga taaagggata gttaaaaact atcccaagct tattgattaa
     1141 gtttttttct acgaagttga ttagaaattt tgaataatca atatttgtct ttttttcgtt
     1201 gatttaaagt ttcttgtcct aattccttcc aaatctgcca tttcatatca tttggatcta
     1261 tttcagcagt tttaagcaga actccaagta actcagctgg atctcgttta tccaagtttg
     1321 caatttcaaa aagtgcgcct aattgaatga ggtgacgtgt acgagcttta cgttcagctt
     1381 ccttttccgc attgattaat tttctaacaa cgactttctg tttatttgct cgatctatag
     1441 ctttttcagc tttgataagg gcatttaatt tcttttcttg ttcaggggtt cggtactgaa
     1501 tttgagccaa ttccaataga agtttttgag catcacttgg attctttaac gcagaaatgt
     1561 aggtgatttt ttcagacagc cattcattat tcatattcaa ttccttaatt catataccgc
     1621 aaaagtacag gctcaattct caaagaacga taacaaaata attacgtcta atgtccgcaa
     1681 ttttgttaaa gttcgttcta gagattgtcg cctgcacttc catcaaaaat ttgatggcgc
     1741 gcttagacgt ttctcaattt cattgagaaa cctaagcacg ttagggactt catccctaaa
     1801 accctgaaac ttattttttt cgtaaatttt caatttacgg aaaaaagaag taccaaagct
     1861 aggagcaaca gcatgcaact aaaacaggta ctgggaaaaa tcaaagatat tacttttggc
     1921 gtgatcgcag caccgctaat catcatcatt atgatgtggg cttcgatact cgatcaaaga
     1981 gcatttaaga aagaactaga ccgaaaaaag gaacaggaaa aaacctaatg gcgattgctc
     2041 gtttaagtgt caaagtcggc aaggcaggca aagcagcacc gcatgccgaa tatattgatc
     2101 gggacgaaga aaagaagctg aaacaggaac aggcagaaac tgaccttgaa catagtgctt
     2161 atggaaacat gccgaaatgg gctgaacata atccgattac cttttgggag gcagccgatc
     2221 tttacgagcg caaaaatggc agtacgtatc gagaatatga aattgccctg cctagagaga
     2281 tgaatgccga gcaacgccta gaactggttg aggattttat tcagtctgaa attggctcaa
     2341 aatatccgta tcagtttgcg attcacaacc ccaaagcaat ggacggcaac gatcagcccc
     2401 acgtccacct catgtttaat gaacgtctgc aagatggaat tgagcgagat ccagagcaat
     2461 attttaagcg ctataacagc aaaaatcctg aacgtggcgg agctaaaaaa gacaatacgg
     2521 gtaaaagtta ccaggaacga aaaaccgata ttaaagacct acgccaaaga tgggcagatt
     2581 tatgcaacag ccatttagaa aaacaccaaa ttgacagccg tattgatatg cgaagctata
     2641 aagagcaagg catagagaaa gaacctgaaa agaaactgtt accaagccaa gctaaagatc
     2701 ctgaaatcag ggaagccctg caacagtcac gcacagccta taaagagctt gagcgactgg
     2761 acttaggcga cccgaaaaaa gacctgaaag aactcaaaga cagcccaatt tcagacaaag
     2821 aaattaagca gggtattgag agctttaagg ctgattttga cagcttcaaa cagcttgctt
     2881 tagagcagta caaagcacag cagaaattag aacgagagca acagaaaaca atgaagttta
     2941 gagggatgag ccgatgacac ctgatgaaaa aaagttatat gcactgatgg cagttgcaga
     3001 acaacagcag aacctagtca atcaggccgt taaagagctt caaatcacga aagaacacat
     3061 tcaaagcact attgctgagc aaacccacca tactgtatca aaatcgctaa atatgggctt
     3121 acaggagggt acagcggagc ttattaagac cgctaaagca cttggacgct tgaataattc
     3181 aattgaatca gcccgtgatc agttgtcatg gaagctcatt accattgttc taggcatctt
     3241 tgccattctg atgcttggta tcatttggct gtttagtgtt tatatcaaac ctttaggcaa
     3301 tatcccgcag atcgagagcc tgagacagca aggcatccag tttgatattg gaagttgtaa
     3361 agttgatgga gaatctaaac cgtgtgtccg agtgatgaaa aagcagtgcg gatatggaaa
     3421 aaatggcgat ttttgcgtca tagatccaaa atgataagta cttgataaag aaaaagccca
     3481 ttcagtattg aaactaaatg ggctttcgct ttatattcag cggtacgcag ttatttgatg
     3541 ctctaacatc gaataacgaa gatcataagg tttggcgacc gttatgattt cacactaagg
     3601 cttgtacctg aactctattt tcacaataaa cagtgtttat atcaagcctc aaaatgataa
     3661 gtcttcgtta attcgtgttt taaatcgtca aataagtatc ctagatcgag ctttgcccgc
     3721 ttctgcgtat ccgttttacg gtcggcatgc agtacgtgtg gtaggaataa gtgtcactgg
     3781 cgtattgtgg ctatagtcga aaggctaaac cgcttacagg ggaacttcac ataggcggag
     3841 cgttgggagt agttacatag gacttgttaa gttgtacgtt ggggaaaccc tcgcagagat
     3901 acaggcaatg taacgacagg gagcgcgtgg agcgaagtgc aagactttga gtccttgtct
     3961 gcatagtcag caaagctatg tgcggatacg accgatatac gtggctccga agccaaatat
     4021 atacaggtaa atcggcaata cttaggggat gtgtcatttt tttaaatgac tgcccttagg
     4081 gtgagtattg ccgaaactct ctcaaccgtt tccaaagcca aagcacgaag tgcgacgctt
     4141 tgggggcgag ttgagaggtt gagagcatat gtttttatag tttatggaga gatagttttt
     4201 tagaaatcag cttttttgct taggctattg ctatgattta aataggttct aacaagttca
     4261 attcaaatac tggggttagg gtggatagaa aagggtctaa ctgctatcat tgcctaactt
     4321 gggaaccaag ttataactgg cgtgtatgct catatattta attcattaaa aaagactaat
     4381 ctcaaaatca gtccttctta aggaattttt tgacaagctg aatgtgcaaa ataaaaaatg
     4441 cacattcaga gtatggatct aatcactagc tatccaagca tatt
//
