LOCUS       NZ_CP021783             2726 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Acinetobacter baumannii strain A85 plasmid pA85-1, complete
            sequence.
ACCESSION   NZ_CP021783
VERSION     NZ_CP021783.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07125723
            Assembly: GCF_002210065.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Hamidian,M., Hawkey,J., Wick,R., Holt,K.E. and Hall,R.M.
  TITLE     Evolution of a clade of Acinetobacter baumannii global clone 1,
            lineage 1 via acquisition of carbapenem- and
            aminoglycoside-resistance genes and dispersion of ISAba1
  JOURNAL   Microb Genom 5 (1) (2019)
   PUBMED   30648939
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Hamidian,M., Kenyon,J.J., Holt,K.E., Pickard,D. and Hall,R.M.
  TITLE     A conjugative plasmid carrying the carbapenem resistance gene
            blaOXA-23 in AbaR4 in an extensively resistant GC1 Acinetobacter
            baumannii isolate
  JOURNAL   J Antimicrob Chemother 69 (10), 2625-2628 (2014)
   PUBMED   24907141
REFERENCE   3  (bases 1 to 2726)
  AUTHORS   Hamidian,M., Wick,R.R., Hawkey,J., Holt,K.E. and Hall,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUN-2017) School of Life and Environmental Sciences,
            The University of Sydney, Cnr., Maze Cres., and Bultin Ave, Sydney,
            NSW 2006, Australia
REFERENCE   4  (bases 1 to 2726)
  AUTHORS   Hamidian,M., Holt,K.E., Pickard,D., Dougan,G. and Hall,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2014) School of Molecular Bioscience, The
            University of Sydney, Sydney, NSW 2006, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-JAN-2017
            Assembly Method                   :: Unicycler v. JAN-2017
            Assembly Name                     :: Acinetobacter A85
            Expected Final Version            :: yes
            Genome Coverage                   :: 369.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 08:03:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,032
            CDSs (total)                      :: 3,936
            Genes (coding)                    :: 3,873
            CDSs (with protein)               :: 3,873
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 28 of 63
            Pseudo Genes (incomplete)         :: 35 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP021783.1.
            Bacteria and source DNA available from Ruth Hall, University of
            Sydney.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="A85"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pA85-1"
                     /geo_loc_name="Australia:Sydney"
                     /lat_lon="33.8650 S 151.2094 E"
                     /collection_date="2003"
                     /collected_by="Ian Carter"
                     /genotype="global clone 1"
     gene            1..1251
                     /locus_tag="CBI29_RS20565"
                     /old_locus_tag="CBI29_04201"
     CDS             1..1251
                     /locus_tag="CBI29_RS20565"
                     /old_locus_tag="CBI29_04201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180320.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_031943495.1"
                     /translation="MQNLDKKKPLLSDSLATGDNKGFASPKGDQHRDRITRFGNLKHRS
                     KQQENYLFSLAKIKENYHADVKNDESIRAMKTAQKLNGCGNFLLFKNFYTIDQIKLAKF
                     QACSEHLLCPFCAGIRASKAIQKYSERVDQVLSENPRLKPVMITFTVKNGVDLRERFTH
                     LIKSFRTLIERRRDYIKKGRGFNEFCKINGAMYSYENTYNEKTNEWHPHIHVFALLDDW
                     IDQDELSQYWQSITGDSMVVDIRRAKKQKDLGYSGAAAEVCKYALKFGDLSVEKTWEAF
                     KVLKGKRLSGAFGSLWGVKIPESLIDDLPDDSDLPYLEMIYKFVFSKKSYYDLQLTRHV
                     EPTGKDAADELRGVEGRNLLVSMDGRGVSDAGRARTGALAPQHGRKKQHWQIPPVTRVR
                     VRKRIRRWDGYLCVLHL"
                     /name="protein rep"
     gene            1348..1563
                     /locus_tag="CBI29_RS19685"
                     /old_locus_tag="CBI29_04202"
     CDS             1348..1563
                     /locus_tag="CBI29_RS19685"
                     /old_locus_tag="CBI29_04202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001262268.1"
                     /translation="MANANTTVLELLGKQVSFVYVLKSDSDEYSFTCSGVVTDVIISLN
                     SELQLSVDNGDFYIYSDLKDFSIKSE"
                     /name="hypothetical protein"
     gene            1872..2189
                     /locus_tag="CBI29_RS19690"
                     /old_locus_tag="CBI29_04203"
     CDS             1872..2189
                     /locus_tag="CBI29_RS19690"
                     /old_locus_tag="CBI29_04203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000070380.1"
                     /translation="MSNPSKLDTLVAEISGTAESEYIGLYKQIPFRVRLPLFARLSALH
                     TIQNSHNKTPRNALLNDLLEIAMDQVMFKLDEETLEALGHLEAQYYEELSQYDSGELSD
                     D"
                     /name="hypothetical protein"
     gene            2352..2642
                     /locus_tag="CBI29_RS19695"
                     /old_locus_tag="CBI29_04205"
     CDS             2352..2642
                     /locus_tag="CBI29_RS19695"
                     /old_locus_tag="CBI29_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000593064.1"
                     /translation="MIKLEGTVLNTYHLDGGTNKKGEEYEARDKVQLLGSLELPNGQIK
                     NELIDLTVEDSRIYDDFKNKLISISCGAMAVGRNVIFYVRKGAKPVLADHL"
                     /name="hypothetical protein"
     Typing          2..1251
                     /name="rep_cluster_420"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.602"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgcagaatt tagataagaa aaaacccctg ttatcggaca gtttggcgac cggtgataac
       61 aagggttttg catctcccaa aggagatcaa catagggata gaataacacg ttttggcaat
      121 ttgaaacata gatcgaagca acaagaaaac tatttatttt cgttagctaa gattaaagaa
      181 aattatcatg ccgatgtaaa aaacgatgaa tctattcgcg ccatgaaaac tgcccaaaaa
      241 ttaaatgggt gcggtaattt tcttctattc aaaaattttt acaccattga ccaaattaaa
      301 ctcgccaagt tccaagcttg tagtgagcat ttgttatgcc cgttttgtgc aggtattaga
      361 gcttctaagg caattcaaaa atactctgag cgtgttgatc aagtcttatc tgaaaatcct
      421 cgtttaaagc ccgttatgat cacgtttacg gttaaaaatg gggtagatct aagggaacgg
      481 ttcactcatc ttataaaatc gtttagaacg cttatagaac gtcgtaggga ctatattaaa
      541 aaagggcgtg gctttaatga attttgcaaa attaacggtg caatgtattc atatgagaat
      601 acttacaatg aaaaaactaa tgagtggcat ccccatattc atgtgtttgc acttttggat
      661 gattggatag atcaggatga attgtctcaa tattggcaat ctattactgg ggactctatg
      721 gtcgttgata tccgtagagc caaaaaacaa aaagacttag gctattcagg tgctgctgct
      781 gaagtctgta aatatgctct caaatttggt gatctttctg tagaaaagac ttgggaagct
      841 ttcaaagttt taaaaggtaa gcgattaagt ggtgcttttg gatctctttg gggcgtgaaa
      901 attcctgaat cattaattga tgatttacca gatgattctg atttacctta tctagaaatg
      961 atttataagt tcgtcttttc taagaagtct tattacgatt tacaacttac tcgtcatgtc
     1021 gaacctacag gtaaggacgc cgccgacgag cttcgaggag tagaaggacg caacctgttg
     1081 gtgagcatgg acgggcgagg agtgagcgac gctgggaggg cccgcactgg cgcgcttgcc
     1141 ccgcagcacg gacgaaaaaa acaacactgg caaatcccac cagttactcg tgttcgggtt
     1201 cggaagcgaa tccgaagatg ggacggatat ttatgtgttt tacatttata gtttttaact
     1261 agttaacaat actagtaagt cggtgaaatc agggagtgcc atccccgatt tcacctaacc
     1321 acaacgtact gtcaaggagt acttatcatg gctaacgcga atactactgt tttagaactt
     1381 ttaggcaaac aagtttcttt tgtttatgtt cttaagtctg attcagatga atattctttc
     1441 acctgttctg gtgttgtgac tgatgtaatt attagtctta actctgaact acagctctct
     1501 gttgataatg gggattttta tatttattcg gatttaaagg atttctctat caagtctgaa
     1561 taaggggctc ttggtatatt tacgaaatct catgccacgc ttgaaactca agggtttcag
     1621 gcgtgttttt tttatagttt cttgagaaac gcgtaagcgt gcattactga aaaagcacct
     1681 aaatcttaga gtgggtgtct acccacgaac tgacataaaa cgatttcaaa ccttccctct
     1741 ctgattacaa gctcccttta gcctaagaac gggagttacc gagctttgct cgccgtgata
     1801 ttgaatagat tttaattttg atatataaat agaatcacaa aatgattcct tttatatata
     1861 aggtaagatt tatgagcaat ccatcaaaat tagatacact tgttgcagaa attagtggta
     1921 ctgctgaatc tgaatatatt ggtctttata agcagattcc ttttcgtgta agacttcccc
     1981 tttttgcacg tttatcagca cttcatacca ttcaaaattc ccataataaa actccgcgta
     2041 atgcgttgtt gaatgactta cttgaaatcg ccatggatca agttatgttt aaattagatg
     2101 aggaaacttt ggaagctctg ggacatttag aagctcaata ttatgaagag ctttctcaat
     2161 atgattctgg agaactttca gatgattaga aaaccacgaa ttataagtaa cacctctatg
     2221 gaaagcctgg atgaagcatg tacttacttt agacaggttc tccaggaaca tttagataaa
     2281 aagagacaaa ttacattttt taccattgct atggtcgatg gtcgtttgaa gttaattgag
     2341 gggataccag aatgattaag cttgaaggta cagttttaaa tacataccac ttagatggtg
     2401 ggactaataa aaagggcgaa gaatatgaag ctcgagataa ggtccagctt ctaggttcgt
     2461 tggagcttcc caacggacaa attaaaaatg aattaatcga ccttactgtt gaagattctc
     2521 gtatttatga tgatttcaaa aataagctta ttagcatcag ttgtggcgct atggctgttg
     2581 gtcgtaacgt tattttttat gttcgaaaag gtgcgaaacc tgttttagca gatcacctat
     2641 gattttccct aaaaaacatc gagttagcga agcgtctcga tgttttttct tttacttgat
     2701 actattgatt aaagcgggga caaaat
//
