LOCUS       NZ_CP021721             9396 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain AR_0128 plasmid tig00000856, complete
            sequence.
ACCESSION   NZ_CP021721
VERSION     NZ_CP021721.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04014969
            Assembly: GCF_002180275.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Conlan,S., Thomas,P.J., Mullikin,J., Frank,K.M. and Segre,J.A.
  TITLE     Whole genome sequencing of Escherichia coli AR_0128
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   Segre,J.A. and Mullikin,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2017) NIH Intramural Sequencing Center, 5625
            Fishers Lane, Rockville, MD 20852, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.3
            Genome Coverage                   :: 124x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002180275.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 04:55:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,253
            CDSs (total)                      :: 5,128
            Genes (coding)                    :: 4,921
            CDSs (with protein)               :: 4,921
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 66 of 207
            Pseudo Genes (incomplete)         :: 140 of 207
            Pseudo Genes (internal stop)      :: 42 of 207
            Pseudo Genes (multiple problems)  :: 37 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP021721.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AR_0128"
                     /db_xref="taxon:562"
                     /plasmid="tig00000856"
     gene            complement(join(7940..9396,1..592))
                     /locus_tag="AM375_RS02165"
                     /old_locus_tag="AM375_02165"
     CDS             complement(join(7940..9396,1..592))
                     /locus_tag="AM375_RS02165"
                     /old_locus_tag="AM375_02165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966094.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_023281053.1"
                     /translation="MQTELIKELNKVLKAFPQFWNGEELQRSMVSDAISQKQPDLIKAL
                     VANEKIRAVYGTDIDGIFIFDFDNLCKFLKYKEYWANSFTKYRNKVGLTSEGKYLDYSS
                     DVVLDFPFKDCVLEGGMTKENQGKDEIYYNEIIARDEIDRLFSPKVFTNVKRHTKNGAQ
                     NNISYFDQSDNLIIKGNNLIALNSLITPHKGEVKLIYIDPPYNTGSDSFKYNDRFNHST
                     WLTFMKNRLETSLKLLSDDGFIFIQCDDNELFHLKVLMDNIFGVENFINIITIKTKIGG
                     VSGSSEGKSLRDATEFIILYSKRKELAMLNPVFKKTEVIQYVNEYKDSGKSWKYTQILL
                     DLGAKTPIKSEDGVTYNHYPYAKTTSISRFSKEKGISENDVYKDYAGIVYRTTNAQSSV
                     RGRVLKELEEINNGIVSVEYTPLKGKNKGTPVEIFYNASNKDMFMFLSDMLEKNGESYN
                     YMQKINTLWDDIQYNNLNKEGGYVSFENGKKPELLIKRVIELSTKAGDLILDFHLGSGT
                     TAAVAHKMGRRYIGIEQMDYINDITVPRLQKVIGGEQGGISKDINWQGGGSFVYAELME
                     LNAYFVHEIQKAQSTEELEKLFAVMKTEAHLNYQVALENVLSAEYEVDGIFRKVAFSEL
                     ELHEQKQLLIEILDKNQLYVNASDMDDSDLNISESDKAFTRSFYGME"
                     /name="site-specific DNA-methyltransferase"
     gene            complement(750..899)
                     /locus_tag="AM375_RS27940"
     CDS             complement(750..899)
                     /locus_tag="AM375_RS27940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152930637.1"
                     /translation="MEILEKIEVSARLLAEIISVVLITNRQHKNSQFWGQKTGCFARFD
                     VFSS"
                     /name="hypothetical protein"
     gene            complement(878..1327)
                     /locus_tag="AM375_RS02170"
                     /old_locus_tag="AM375_02170"
     CDS             complement(878..1327)
                     /locus_tag="AM375_RS02170"
                     /old_locus_tag="AM375_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_023281052.1"
                     /translation="MALVSISEAARLTGKSRTTVQSYIKQGKLTKCTDSSGSSKLDTSE
                     LLRVFGSFTGQQLGSEQSGSTVQSLAGESVQLTDQNLLHENYALKAEIELVKALLREKE
                     LLLAEKDKRNEDLKQALLLIESKLPATPEPVTQPMVKKSWKFWKK"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1335..2312)
                     /locus_tag="AM375_RS02175"
                     /old_locus_tag="AM375_02175"
     CDS             complement(1335..2312)
                     /locus_tag="AM375_RS02175"
                     /old_locus_tag="AM375_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_087758688.1"
                     /translation="MAELVVFKANELAVSRYDLTEHETKLILFCVAKLNPSLETPDETE
                     RTVKFSCSEYASFMGISYENAWGRLNTSTRELFKRSIELIYPTGAVSMRVFNWAEYAEF
                     NRETQEVTLVFSKYIIPLLFHLKRFIKYNLDHVKSFENKYSMRIYEWLLKELTQRKTHK
                     ANISIRIDEFKFMLMLENSYPAYKLLNHWVLKPISKDLNSYSNMKLAIEKRGRPADTLI
                     FQVELDKQIDLVTELAKADPATAPEKTTRTPIPVTNSTQDSQLHNGLKKTLHDALTAKI
                     QLTSFEAKFLSDMQSKHDLNDSFTWLTQKQRATLEKILAKYGRF"
                     /name="replication initiation protein"
     gene            complement(2928..3380)
                     /gene="traD"
                     /locus_tag="AM375_RS02180"
                     /old_locus_tag="AM375_02180"
     CDS             complement(2928..3380)
                     /gene="traD"
                     /locus_tag="AM375_RS02180"
                     /old_locus_tag="AM375_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_023281057.1"
                     /translation="MSKSEKQEKWVADRVQYIRGLKSPNEQQRLMVLLTDKVNRTAQDI
                     KTLSLLIQAEKAAEKAQEARTKVMNLIQAEKRAEARAARKARDHALYQSAGLLIMAGLV
                     DSQTGKPVDDAAALLGALASLNDLSRDNPKWSDWKIRGQELLNSRK"
                     /name="traD"
     gene            3544..4659
                     /gene="mobA"
                     /locus_tag="AM375_RS02185"
                     /old_locus_tag="AM375_02185"
     CDS             3544..4659
                     /gene="mobA"
                     /locus_tag="AM375_RS02185"
                     /old_locus_tag="AM375_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_023281056.1"
                     /translation="MASYHLSVRTGGKGNASPHADYISREGKYARKKDADLEHKEAGNM
                     PAWAAHNPSEFWKAADAFERANGCTYREIEIALPRELNPAQRLELVRAFVSQEIGDRHA
                     YQFAIHNPTAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKVPERGGAKKVRFG
                     ETPTERKEHLIAQRERWADLQNAYLERYQHADRVDARSLKAQGIGREPERHLGAGQVQR
                     FDTDQLQAILERREAERQVQQCCDERDSVIDVTTSLREAISERDTLMLKQTQKSDPEQD
                     AVSGRVFDFEKEPEKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQEIHQEMERQKERAR
                     LAEKQRQQEKERQRIAEQKRQKPDKGWSFSR"
                     /name="mobA"
     gene            4672..5256
                     /locus_tag="AM375_RS02190"
                     /old_locus_tag="AM375_02190"
     CDS             4672..5256
                     /locus_tag="AM375_RS02190"
                     /old_locus_tag="AM375_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_023281055.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVSTLTEEFQAASVQLQRQSETLSK
                     VIRELDSASGNMTDTVRKSVSSAMNQVTQALTQAGTEQQKPAVNALNQVVKAANDSVNA
                     MRREMYRYTWKSAIYLVLTIFFVLACCVTALTWFMNDGYSRIAEMQRMEAVWQKKAPLA
                     DISTCDGKPCVKVDTRTTYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(5299..7935)
                     /locus_tag="AM375_RS02195"
                     /old_locus_tag="AM375_02195"
     CDS             complement(5299..7935)
                     /locus_tag="AM375_RS02195"
                     /old_locus_tag="AM375_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443154.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0016787 -
                     hydrolase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="WP_023281054.1"
                     /translation="MAKKPTSAGSEKLLFNKLKQFDEPGLFHDNYQTPDYIQANLKDAL
                     RPYQHGALRYLHYTQRKREEALLHYRHLLFHMATGAGKTMVMAGTILYLFKEYGYQNFI
                     FFVHTDAIIQKTRENLLNPQSPKYLFSQELEIDGEKITIAPVETFPSVPERNTIYLKLS
                     TIHKMHDELNSYRENSITYEDLKEIPLVLLGDEAHHFNAGTKARGKSKTSPENEEQTWE
                     RTIENILDLRPDNRLFEFTATIDLANKDIGQKYRDKVVYQYDLKQFMSDGYSKKVMLLE
                     ANQNDGDKMLDAVLLSQYRKLTAADNGITGFKPVILFKSNKIAISKAKQEEFSQLIAAM
                     TPESVRRHLANKRVQLSSDTSIWHKVIQRYADSDLVTVIQQIQEDFNDFNLLNVNKSDL
                     LEENPVLLNTLENIDNPVRAVFAVAKVNEGWDVLNLYDIVRISEQASSSKTGTDSEAQL
                     IGRGARYYPFVYDGKRSFTRRFDNSAKDLSVLEQLHYHTINEPAYIKTLHASLEQADID
                     VHQDGSGTIEHARLKEDFKKSTVYQTGKLYFNKVEEIESSSRRWETYSLETRFEIPYQT
                     AGEESLDNLTGATAVITKPEPLVLDERFYRKAMQRISFYALDNLQRFFPKLTGIREFIR
                     SDAYLGKLKITVTVPQSLDFSSVPAKEKLHLLETVLLRISENIRRNDQKVKGTYRFISQ
                     PVKEVIKDYSLHIDPSVVINQKITTAPTIGKKWYVYDNAILNQLEHRLVKTLEAFMPKL
                     KARYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILTRHSDNTYWQVFLEPKGDDRL
                     LDDAWKEQMLETLNDRERIVIDENEDVRLVGIKFFANSQMDAFVSDMQNRLNEGESLET
                     ASFSLPL"
                     /name="DEAD/DEAH box helicase family protein"
     Typing          complement(1336..2036)
                     /name="rep_cluster_1787"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3545..4656
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.097"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actttacctc gcctttatgg ggggttatta atgaattaag ggcaattaag ttatttcctt
       61 tgatgattaa attatcactt tgatcaaagt aagatatatt gttttgtgca ccattttttg
      121 tatgtcgttt aacattagtg aatacctttg gagagaaaag tcgatcaatc tcatcacgag
      181 caatgatttc attgtaataa atctcatctt tcccctgatt ttctttggtc attccgcctt
      241 caagaacaca atccttaaaa ggaaagtcca gcaccacatc agagctgtaa tcaaggtatt
      301 tcccttcact ggttaatccc accttgttcc ggtatttagt gaagctgttc gcccagtatt
      361 ctttgtattt aagaaatttg cataaattat caaaatcaaa aataaaaatt ccgtctatat
      421 ccgtaccgta aacagcgcgg attttttcat tcgccaccaa tgccttgata aggtctggct
      481 gcttttgact gatagcgtca ctcaccattg aacgttgtaa ttcctctccg ttccagaact
      541 gcgggaatgc ttttaaaacc ttgttaagtt cttttattaa ttctgtctgc atgtcatgac
      601 cttatgactg tttaatatct gaatgattgt aagatatagc gtccggcgct ggaacccgac
      661 agcgatgagt tgaattcagt tgcgggaaaa tgcaccagaa taagtggtat ttaacataaa
      721 ctctcttacg cgcaccaccg atcaggtact cacgacgaaa acacgtcaaa acgcgcaaaa
      781 cagcctgttt tctgccccca aaattggctg tttttgtgtt gccggtttgt tatcaaaacg
      841 acggatatga tttcagccag caaacgggct gaaacctcta ttttttccag aatttccatg
      901 attttttcac catcggctgc gtgacaggtt ctggtgttgc cggtagcttg gactctatca
      961 gtaacagggc ttgcttgaga tcctcgttgc gcttgtcttt ttcagcgagt aggagttctt
     1021 tttcacgcag caatgccttt acgagttcta tttcagcttt taaagcatag ttttcatgca
     1081 gcaaattctg gtcagttaac tgtacggatt ctcctgccaa actctgcaca gtgctgcctg
     1141 attgttcact gccaagttgc tgaccagtaa atgatccgaa aacacgcaga agctctgatg
     1201 tatctagctt tgaagaacca ctgctatctg tgcacttggt cagttttccc tgcttaatgt
     1261 atgactgcac tgttgtgcga ctttttcctg tcagtcttgc ggcttcgctg attgatacca
     1321 gtgccataga tacctcagaa ccgtccgtat ttagctaaaa ttttctctag cgtggctcgt
     1381 tgtttttggg tcagccatgt aaatgagtcg ttgagatcgt gcttgctttg catatcgctt
     1441 agaaatttag cctcaaagct agtgagctgg atttttgcgg tcagtgcatc gtgtagtgtc
     1501 tttttaagac cgttatgcaa ttgactgtct tgcgtgctgt ttgtcaccgg aatgggcgtt
     1561 cttgttgttt tttctggtgc tgtggcggga tctgctttgg ctaactcagt tactagatca
     1621 atctgtttgt ctaactcaac ctgaaagatc agggtgtcag caggacggcc tcgtttttct
     1681 attgctaact tcatgttgct gtaggaattt aaatcttttg agattggttt taaaacccaa
     1741 tggtttagta atttatatgc tgggtagcta ttttctaaca tcaacatgaa tttaaattca
     1801 tcaattctta ttgaaatatt ggctttatgt gtttttcttt gtgttagttc ttttagcagc
     1861 cattcataaa tgcgcattga gtatttgttt tcaaaagact taacatgatc gaggttgtac
     1921 ttaatgaatc tctttaggtg aaatagcaat gggattatgt atttactaaa aactaaagtt
     1981 acctcttgtg tttccctatt gaactctgca tattcagccc aattaaaaac gcgcatagat
     2041 acagcacctg ttggatatat taattcaata gagcgcttaa ataattctcg tgttgatgta
     2101 tttaatcgcc cccacgcatt ctcataactt atccccataa atgaagcata ttctgagcat
     2161 gaaaatttaa ccgttcgttc agtttcatct ggtgtttcta atgaggggtt tagctttgca
     2221 acacaaaaca ggattagttt cgtttcgtgc tctgttaggt cataacgact aactgcaagc
     2281 tcatttgctt tgaatactac caattcagcc atgactaaaa ccattggttg tgattttaaa
     2341 ataacataac ctttaccatt ggttatgtca attgcactgg cataaccttt ttggaggttg
     2401 tgtggattac ctaattttca cttaaccttt tggccgggac ccctaatttt tgcttaacct
     2461 ttcctaattt ttgcttaacc ttttgcgttt ttttttgttt tataacaata acttaaaatc
     2521 cttagaataa aaaaagaata aaaaaagata aaaaagaata gatcccagcc ctgtggataa
     2581 ctgaccacca gagacaattg ttcagtattg ataacaggat gtcgcaaacg ctgtttgctc
     2641 ctcttcaaaa cagatctctt aaccccgaaa cactgaggta gctctccctt cgtaagctcg
     2701 ggcaactcgc tgaacatttc tttcgtctcc gaccatccgg cacccgaatc gcggttgttt
     2761 tcgtgaaaat cagttcgctt cgctcacggc tctggcagga aatggggtta aatggcgcta
     2821 cagacgtctt tcatgggtta cagtgaagga ttccacccat aaaacaaaaa aagcccgtca
     2881 cgggcttctc agggcgttta atcgctatct gctcaggtgt tcctgtctca cttcctgctg
     2941 ttcagcagtt cctgccccct gattttccag tctgaccact ttggattatc ccgtgacagg
     3001 tcattcagac tggctaatgc gcccagcaga gcagccgcat catcgaccgg ttttcccgtc
     3061 tgactgtcta ccagacccgc catgatgagt aatccggctg actggtacag cgcatggtca
     3121 cgggctttac gggcggctct ggcttcagcc cgtttctctg cctgtatcag gttcatgacc
     3181 tttgttctgg cttcctgtgc tttctctgcg gccttttctg cctgaatcag cagtgacagt
     3241 gttttgatat cctgcgctgt cctgttcact ttatccgtca gcaataccat caggcgctgt
     3301 tgttcattcg gcgactttaa accgcgaata tactgcactc tgtctgccac ccatttttcc
     3361 tgtttttcgc ttttactcat acccgtctgt cctctgcgtt ttaacggtgg aattatgctc
     3421 actggtagct gcaaaggtca aattccacta caatccctga taagttttta tttatcaggg
     3481 cgcacttaga cgtttcttca ctttgttcag aaacggtgcg cgccttccgc gggataaaac
     3541 acaatggcct cctatcatct cagcgtcagg accggcggga aaggtaacgc ttccccccat
     3601 gccgattaca tttcgcgtga gggaaagtat gcccgcaaaa aagacgccga ccttgaacac
     3661 aaggaggccg gcaacatgcc ggcatgggcg gcgcataatc cgtctgaatt ctggaaggcg
     3721 gcagacgcct ttgaacgcgc caacggctgc acctatcgag agattgaaat tgccctgccg
     3781 cgtgagctta atccggcaca gcgtcttgaa ctcgtccgtg cgttcgtcag ccaggaaatc
     3841 ggcgaccgtc atgcgtatca gtttgccatc cataacccga cagcggcgat tgccggcggc
     3901 gaacagccgc acgcgcatat tatgttttct gaacggatga atgacgggat tgaccgtgac
     3961 ccgcagcagt atttcaaacg ggcaaactct aaagtaccgg agcgcggcgg agctaaaaag
     4021 gtgcgtttcg gggaaacacc gacagagcgc aaagagcatc ttatcgccca gcgtgaacgc
     4081 tgggctgacc tccagaatgc ctatcttgaa cgctatcagc atgctgaccg tgtcgatgcc
     4141 cgctccctga aagcgcaggg catcgggcgt gaaccggagc ggcatctggg ggcgggacag
     4201 gttcagcgct tcgatactga ccagttacag gccattcttg agcgcaggga agcggaaaga
     4261 caggttcagc agtgctgtga tgaacgtgac agcgtgattg acgtaaccac ctcgctgcgg
     4321 gaagccatca gtgagcggga cactctgatg ctaaaacaga cgcagaaatc tgatcccgaa
     4381 caggacgccg tttccggcag ggtattcgat tttgaaaaag agccggagaa gctgaatgca
     4441 ctggtcagcg atgccatgaa ggacatacag gaagaaattg accttcagtc ccttgttaac
     4501 gatgcgatgg cggagtttca ggagatacat caggaaatgg aacggcagaa agagcgggcg
     4561 agactggcgg aaaaacagcg tcagcaggag aaagagcggc agcgcattgc cgaacagaaa
     4621 cgacagaagc ccgataaagg ctggtcattc tcacgataag caggatttga tatgagcgac
     4681 gaaattaaac agattgctgc ccttatcggg catcatcagg cgctggaaaa acgagtgagc
     4741 acgctgactg aggagtttca ggctgcctcc gtgcagttac agcggcagag cgaaacactg
     4801 agcaaggtta tccgggagct ggacagtgcg tccggtaata tgaccgatac agtcagaaag
     4861 tctgtcagct ccgctatgaa tcaggttacg caggcgttaa cacaagccgg cactgagcag
     4921 cagaagcccg ccgtgaatgc ccttaatcag gtcgtgaaag cagctaatga ctccgttaac
     4981 gccatgcgcc gtgaaatgta tcgctatacg tggaaatcgg cgatttatct tgtgctgacg
     5041 atattcttcg tcctggcttg ctgtgtgacg gcactgacat ggtttatgaa tgacgggtac
     5101 agccggattg ctgaaatgca gcgtatggaa gctgtctggc agaaaaaagc gccgctggct
     5161 gatatctcta cgtgtgacgg aaagccctgc gttaaggtgg acacccgcac cacgtatggg
     5221 gataaagaga atacctggat gattattaag aaataaaata acggagcatt acgctccgct
     5281 attgggtgtt gttgcgacct acaacggcag ggagaacgag gcggtttcga gcgattctcc
     5341 ctcattcagc ctgttctgca tgtcgctgac gaaggcatcc atctgactgt tggcaaagaa
     5401 tttaatcccg accagcctga cgtcctcatt ttcatcaatc acaatgcgct ccctgtcatt
     5461 cagcgtttcc agcatctgct ctttccatgc gtcatccagc agccggtcat ccccctttgg
     5521 ctcaaggaac acctgccagt aagtgttgtc cgaatggcgc gtcagtatca tgatgaaatc
     5581 cggcataaac ccgcgcacgc cgccgaactc cgtgagttta aagcgcgttg actgctcgtc
     5641 gttacgcagt acgtaaatgt cgtcgtaacg cgctttcagc ttcggcatga atgcttccag
     5701 cgttttcaca aggcgatgct caagctggtt cagtatcgcg ttatcgtaca cgtaccattt
     5761 tttaccgatg gtcggtgcgg tggtgatttt ctggttaatg accaccgacg ggtcaatgtg
     5821 caggctgtag tctttaatca cctccttcac cggctggctg ataaagcggt acgtgccttt
     5881 caccttctgg tcattgcggc gtatgttctc tgagatgcgc agcagcacag tttccagcag
     5941 gtgcagtttc tcttttgccg gtacagagga gaaatccagt gactgcggta cggtgacggt
     6001 gatttttagc ttgccaagat aggcgtcact gcgaataaac tcacggatgc cggtcagttt
     6061 cgggaagaag cgttgcaggt tatccagcgc gtagaagctg atcctctgca tcgccttgcg
     6121 gtaaaaacgc tcgtccagta ccaggggttc cggttttgtt atgactgccg ttgcgcccgt
     6181 caggttgtcc agcgactcct caccggcggt ctgatacggt atctcaaagc gtgtttccag
     6241 cgagtaggtt tcccagcgcc ggctgctgct ctcaatctct tccactttgt tgaaatacag
     6301 cttacccgtc tgatagaccg tggatttctt gaagtcttct ttcagtctgg cgtgttcaat
     6361 cgtgccgctg ccatcctgat gcacgtcaat gtccgcctgc tctaatgatg cgtgcagcgt
     6421 ttttatataa gctggttcat tgatggtgtg atagtgcagt tgctccagca cactcaaatc
     6481 tttcgcgctg ttatcaaacc ggcgggtgaa gctgcgctta ccgtcataaa cgaacgggta
     6541 gtaacgcgca ccacgcccga taagctgcgc ctcgctgtcc gtgccggttt tgctgctgct
     6601 cgcctgctcg ctgatgcgga caatgtcgta gaggttgagc acgtcccagc cttcgttgac
     6661 cttcgccacg gcaaagacgg ctctcaccgg attatcgatg ttttccagtg tgttcagcag
     6721 gacggggttt tcttccagca ggtctgactt gttgacgttc agcaggttga agtcgttaaa
     6781 gtcttcctga atctgttgga taaccgtcac taaatcgctg tcagcgtaac gctgaatcac
     6841 tttgtgccag atactggtgt cagagctgag ctgtactctt ttgttcgcca ggtggcgtct
     6901 gacgctctca ggcgtcattg ccgcaatcag ttgcgaaaac tcctcctgct tcgcctttga
     6961 tatcgcaatc ttgttcgact tgaacagaat gaccggcttg aagccggtga tgccgttatc
     7021 tgctgctgtc agtttacggt actggcttaa cagtaccgca tcgagcatct tgtcaccgtc
     7081 attctggttg gcttccagca gcatgacttt cttcgaatag ccgtctgaca tgaactgctt
     7141 caggtcatac tgataaacca ctttgtcacg gtatttttgc ccgatatctt tatttgccag
     7201 gtcaatggtg gcggtgaact cgaacagccg gttatccggt cgcaggtcga ggatgttctc
     7261 aatcgtgcgt tcccaggtct gctcctcgtt ctccggtgag gttttcgact ttcctctggc
     7321 tttggtgccg gcgttgaagt gatgcgcctc gtcaccaagc agcacaagcg gaatttcctt
     7381 caaatcctcg taggtgatgc tgttctcacg atagctgttc agctcatcgt gcatcttatg
     7441 gatggttgag agcttaaggt aaatcgtgtt gcgttccggt acggacggga acgtctccac
     7501 cggtgcgatg gtgatttttt ccccgtcgat ttccagctcc tgactgaaca ggtacttcgg
     7561 cgactgcgga ttcagcaggt tctcacgggt tttctggata atcgcgtcgg tatgcacgaa
     7621 gaagatgaaa ttctgatacc cgtactcttt aaacagatag agtatcgtcc ccgccatcac
     7681 catcgttttc cctgcaccgg ttgccatgtg aaacagcaga tgacggtaat gcagcagcgc
     7741 ctcttcacgt ttacgctgcg tgtaatgcag gtaacgcagt gcaccgtgct gatacggacg
     7801 cagggcatcc ttcagattcg cctgaatgta gtccggcgtc tggtagttat cgtggaacag
     7861 ccccggttca tcaaattgct tcagcttgtt aaacagcagc ttctcgctgc cggctgaggt
     7921 cggtttcttc gccatgccgt cactccattc cgtagaagct gcgggtaaag gctttatcgc
     7981 tctctgaaat gttcaggtca ctgtcatcca tatcggaagc gttcacatag agctgatttt
     8041 tatcgaggat ttcaatcagc agttgcttct gctcatgcag ttccagttcg ctgaaggcga
     8101 ctttacggaa aataccatcc acttcgtact cagccgacag cacgttctcc agtgccacct
     8161 gatagttgag atgtgcctct gttttcatca cggcaaagag cttttcgagt tcttcagtgg
     8221 actgcgcttt ctgtatttca tgcacaaagt aggcgttcag ctccatcagt tcagcgtaga
     8281 caaagctgcc gccaccctgc cagttgatgt cttttgagat ccccccctgt tcacctccga
     8341 tgactttttg caggcgtggt accgttatgt cgttgatgta atccatctgt tcaataccaa
     8401 tgtagcgacg ccccatctta tgggctactg ctgcggttgt tccgctacct aaatgaaagt
     8461 ctaagattaa atcgcctgcc tttgtagata gctctatgac ccttttaatt aacaactctg
     8521 gtttctttcc gttttcgaaa cttacatatc caccttcttt atttaaattg ttatattgga
     8581 tgtcatccca taaggtattt attttctgca tgtagttata actttcacca tttttctcca
     8641 gcatgtcgga taggaacata aacatatctt tattgctggc gttgtagaat atttcaactg
     8701 gcgttccttt gtttttccct tttaatggtg tgtactctac gctaacaatg ccattattta
     8761 tttcttctag ttcttttaga actcggcctc tgacactgga ttgtgcattt gttgttctat
     8821 aaacaatccc tgcatagtct ttatatacgt cgttttcact tatgcctttt tcttttgaaa
     8881 atctagatat ggaggtggtt ttggcatagg ggtagtgatt gtaagtaact ccatcttcac
     8941 tttttatggg cgtttttgca cctaaatcta agagtatttg agtgtatttc cagctcttac
     9001 cgctatcctt gtattcattc acatactgaa ttacctctgt ttttttgaaa acagggttaa
     9061 gcatggcaag ttcttttctt ttcgaatata aaattataaa ttcagttgca tctcttaagc
     9121 tttttccttc cgagcttcca gatacaccac ctatctttgt ttttattgtt ataatattga
     9181 tgaagttttc tacaccaaat atgttatcca ttaacacctt caaatggaat aactcgttat
     9241 catcacattg tataaaaata aacccatcat ctgataataa tttaagtgat gtttcaagcc
     9301 tatttttcat gaatgttaac cacgtggagt ggttaaatct atcattatat ttaaagctat
     9361 cggaaccagt gttatatgga gggtcaatat atatta
//
