LOCUS       NZ_CP021102             8639 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Escherichia coli strain 13P477T plasmid p13P477T-8, complete
            sequence.
ACCESSION   NZ_CP021102
VERSION     NZ_CP021102.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06219553
            Assembly: GCF_002951655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Ho,P.L., Lau,A., Liu,C.J., Lai,E.L.Y., Chow,K.H., Tse,C.W.S. and
            Que,T.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Microbiology, The University of Hong Kong,
            102 Pok Fu Lam Road, Hong Kong, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. Nov-2016
            Genome Coverage                   :: 200
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 04:11:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,487
            CDSs (total)                      :: 5,362
            Genes (coding)                    :: 5,063
            CDSs (with protein)               :: 5,063
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 299
            CDSs (without protein)            :: 299
            Pseudo Genes (ambiguous residues) :: 0 of 299
            Pseudo Genes (frameshifted)       :: 156 of 299
            Pseudo Genes (incomplete)         :: 162 of 299
            Pseudo Genes (internal stop)      :: 54 of 299
            Pseudo Genes (multiple problems)  :: 69 of 299
            Pseudo Genes (short protein)      :: 4 of 299
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP021102.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="13P477T"
                     /isolation_source="stool"
                     /host="pig"
                     /db_xref="taxon:562"
                     /plasmid="p13P477T-8"
                     /geo_loc_name="Hong Kong"
                     /collection_date="2013-04"
     gene            join(8561..8639,1..179)
                     /locus_tag="BWI89_RS30355"
     CDS             join(8561..8639,1..179)
                     /locus_tag="BWI89_RS30355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112256.1"
                     /note="Members of this family include Cki (colicin K
                     immunity protein) for the pore-forming toxin Cka (colicin K
                     activity protein), as well as immunity proteins from other
                     related colicin systems.; Members of this family include
                     Cki (colicin K immunity protein) for the pore-forming toxin
                     Cka (colicin K activity protein), as well as immunity
                     proteins from other related colicin systems; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cki family colicin immunity protein"
                     /protein_id="WP_158666934.1"
                     /translation="MTVKYYLHNLLESLIPWLFYLLLNYKTGLSDSGYCRPKRTGATAA
                     VPVSEDLTFSMAVHPGRPYASMTQVVFPHNVPAAAHVPHP"
                     /name="Cki family colicin immunity protein"
     gene            262..454
                     /locus_tag="BWI89_RS28420"
                     /old_locus_tag="BWI89_28405"
                     /pseudo=""
     CDS             262..454
                     /locus_tag="BWI89_RS28420"
                     /old_locus_tag="BWI89_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(643..879)
                     /locus_tag="BWI89_RS30600"
                     /old_locus_tag="BWI89_28410"
     CDS             complement(643..879)
                     /locus_tag="BWI89_RS30600"
                     /old_locus_tag="BWI89_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033806376.1"
                     /translation="MEKAPVPRPFPGSSFFAHMFFPGLSPDSVDNGVTAFEKGESARRS
                     RTTERSESVSEEAEEHRKLHFLLTHLCGISHSA"
                     /name="hypothetical protein"
     gene            complement(1525..1656)
                     /gene="ctl"
                     /locus_tag="BWI89_RS28435"
                     /old_locus_tag="BWI89_28415"
     CDS             complement(1525..1656)
                     /gene="ctl"
                     /locus_tag="BWI89_RS28435"
                     /old_locus_tag="BWI89_28415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 lysis protein"
                     /protein_id="WP_072023375.1"
                     /translation="MKITFLIITILSLSGCQANYISDVQGGTVAPSSSSELTGIAVQ"
                     /name="ctl"
     gene            1681..1972
                     /gene="cti"
                     /locus_tag="BWI89_RS28440"
                     /old_locus_tag="BWI89_28420"
                     /pseudo=""
     CDS             1681..1972
                     /gene="cti"
                     /locus_tag="BWI89_RS28440"
                     /old_locus_tag="BWI89_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 immunity protein"
                     /name="cti"
     gene            complement(1969..3441)
                     /gene="cta"
                     /locus_tag="BWI89_RS28445"
                     /old_locus_tag="BWI89_28425"
     CDS             complement(1969..3441)
                     /gene="cta"
                     /locus_tag="BWI89_RS28445"
                     /old_locus_tag="BWI89_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin 10"
                     /protein_id="WP_033801039.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGG
                     GGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLK
                     DIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAE
                     RQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAES
                     DVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALN
                     SAVSIRDAKKTEVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQ
                     DLAEKAKGSKFNSVDEALAAFEKYKNVLDKKFSKVDRDDIFNALESITYDEWAKHLEKI
                     SRALKVTGYLSFGHDVWDGTLKGLKTGDWKPLFVTLEKSAVDFGVAKIVALMFSFIVGA
                     PLGFWGIAIITGIVSSYIGDDELNKLNELLGI"
                     /name="cta"
     gene            3686..3976
                     /locus_tag="BWI89_RS28450"
                     /old_locus_tag="BWI89_28430"
     CDS             3686..3976
                     /locus_tag="BWI89_RS28450"
                     /old_locus_tag="BWI89_28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(3968..4246)
                     /locus_tag="BWI89_RS28455"
                     /old_locus_tag="BWI89_28435"
     CDS             complement(3968..4246)
                     /locus_tag="BWI89_RS28455"
                     /old_locus_tag="BWI89_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            4368..4790
                     /locus_tag="BWI89_RS28460"
                     /old_locus_tag="BWI89_28440"
     CDS             4368..4790
                     /locus_tag="BWI89_RS28460"
                     /old_locus_tag="BWI89_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_021539380.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5274..5507)
                     /locus_tag="BWI89_RS29525"
     CDS             complement(5274..5507)
                     /locus_tag="BWI89_RS29525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5514..6074)
                     /locus_tag="BWI89_RS29530"
     CDS             complement(5514..6074)
                     /locus_tag="BWI89_RS29530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_074167501.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<6109..6789)
                     /locus_tag="BWI89_RS30605"
                     /pseudo=""
     CDS             complement(<6109..6789)
                     /locus_tag="BWI89_RS30605"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6752..7075)
                     /locus_tag="BWI89_RS28480"
                     /old_locus_tag="BWI89_28460"
     CDS             complement(6752..7075)
                     /locus_tag="BWI89_RS28480"
                     /old_locus_tag="BWI89_28460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7143..7334
                     /locus_tag="BWI89_RS28485"
                     /old_locus_tag="BWI89_28465"
     CDS             7143..7334
                     /locus_tag="BWI89_RS28485"
                     /old_locus_tag="BWI89_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_148119661.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELNADDQADICEALHDHA
                     DELYRSCLARFGDNGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7523..7759)
                     /locus_tag="BWI89_RS30610"
                     /old_locus_tag="BWI89_28470"
     CDS             complement(7523..7759)
                     /locus_tag="BWI89_RS30610"
                     /old_locus_tag="BWI89_28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033806376.1"
                     /translation="MEKAPVPRPFPGSSFFAHMFFPGLSPDSVDNGVTAFEKGESARRS
                     RTTERSESVSEEAEEHRKLHFLLTHLCGISHSA"
                     /name="hypothetical protein"
     gene            complement(8405..8536)
                     /gene="ctl"
                     /locus_tag="BWI89_RS28500"
                     /old_locus_tag="BWI89_28475"
     CDS             complement(8405..8536)
                     /gene="ctl"
                     /locus_tag="BWI89_RS28500"
                     /old_locus_tag="BWI89_28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 lysis protein"
                     /protein_id="WP_072023375.1"
                     /translation="MKITFLIITILSLSGCQANYISDVQGGTVAPSSSSELTGIAVQ"
                     /name="ctl"
     Typing          complement(541..628)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="2.44e-33"
     Typing          complement(7421..7508)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="2.44e-33"
     Typing          complement(1086..1273)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.767"
                     /coverage="100.0"
                     /evalue="1.02e-80"
     Typing          complement(7966..8153)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.767"
                     /coverage="100.0"
                     /evalue="1.02e-80"
     Typing          complement(2..318)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="100.0"
                     /evalue="6.690000000000001e-56"
     Typing          complement(5168..6762)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.436"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7135..7337)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.059"
                     /coverage="100.0"
                     /evalue="2.29e-14"
ORIGIN
        1 ccgggctatc tgattcaggt tattgccgac cgaagcgaac tggcgcaaca gcggcggtgc
       61 cagtgtcgga agacctgacg ttttcgatgg cggtgcaccc aggcagacct tacgcatcca
      121 tgacgcaagt tgttttccct cacaacgtgc cagcagccgc gcatgttcct catccgtgac
      181 ccgtatagtg agcatccttt cgcgtttcac cggtatcatt aaaacctccg acagactccc
      241 cacacatgga gaaacagaac tgtgactaaa caggaaaaaa cagcccttaa catggcccgc
      301 tttatcagaa gccagacgct gacactgctg gaaaaaactg aatgaactga acgccgacga
      361 ccaggcggac atctgcgaag cccttcacga ccacgccgac gagctttacc gcagctgcct
      421 cgcacgcttc ggggataacg gtgaaaacct ctgacacata cagctcccgg agacggtcac
      481 agcttgcctg tgagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcaggttt
      541 tagcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
      601 cttataccat gcagaatcag tgcggattgt atgaaaagtg catcatgccg agtgtgaaat
      661 gccgcacaga tgcgtaagga gaaaatgcag cttccggtgc tcttccgctt cctcgctcac
      721 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggac tcccctttct caaaggcggt
      781 aaccccgtta tccacagaat ccggggataa cccgggaaag aacatgtgag caaaaaacga
      841 agaccccggg aaaggccgcg gcacaggcgc tttttccata ggctccgccc ccctgacgga
      901 cttcacaaaa cagacacttt ttcggtgcat ctgtgggagc cttacggctc aaccatgaaa
      961 tccgccagcg gtggcgaaac ccgacgtggc ttaaagatca tcaccgtttc cggtgaatcg
     1021 ctccctcctg cgcccccacg ttcccgccat atcgattacc ggataccttt tccgcctttc
     1081 tcccttgcgg gacacgtggc gctttctcat agctcacgtc ttctggtatc tcggggcgac
     1141 ttaggcagtt cgctccgggc cgggctgtat gcaggaaccc cccgttcagc ccgactgctg
     1201 cgccttctcc ggtaactgtt ctcttgggtc caacccggaa aggacacgac ttaacgccag
     1261 tggcagcagc cattggtagc caggttagca gagcgagatt tataagttag taacagagtt
     1321 cttgaagtat cagcctgatt ttgactacac tagaaggaca agttttgggt atctgcgctc
     1381 tgctgaagcc agttaccttc ggaaaaagag ttggtagatc ttgatccggc aaacaaacca
     1441 ccgttggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1501 ctcaagaaga tcctttgatc ttttctactg aaccgcgatc cctgtcagtt cagaagagga
     1561 agatggagcg accgtccctc cttgtacatc actaatataa tttgcttgac atcccgacaa
     1621 ggataatatt gtaattatca aaaacgttat tttcattcta cccctataac aacataaata
     1681 atgaccgtaa aatactacct acacaattta ctagagtcac ttatcccatg gctattctat
     1741 ttgttattaa actataagac gcccccattt tcacttttca tattcatcgc atctatacat
     1801 gtattactat acccatattc aaaactaact atattttctt ttatacaaaa cacgacaaaa
     1861 atgaaaaaag aaccttggta ctcatacaac cttttttatt ttctttacct agcaatggca
     1921 atcccagtag gattgcccag ttttcatata ttactctcta aagagaaatt aaatacctag
     1981 caattcatta agcttgttca actcatcatc ccctatgtaa gaagaaacaa tacctgtgat
     2041 aattgcaatt ccccagaagc caagaggcgc accaacaata aaactaaaca ttaatgccac
     2101 aatttttgcc acgccgaaat ctaccgcgct cttctccaga gtgacaaata aaggcttcca
     2161 gtctcctgtt tttaatccct ttagggtacc atcccataca tcatgcccga aagacaaata
     2221 tccagtaacc ttaagagctc tagagatctt ttctagatgc ttggcccact catcataagt
     2281 aatagactct aaagcattaa aaatatcatc cctatcaacc ttactgaatt tcttatccag
     2341 tacattttta tacttttcaa atgcagcaag tgcttcatca acactattaa atttactacc
     2401 cttcgccttt tcagctaaat cctgagctat tctggaatat ttttccccat attgttcggt
     2461 tatatattga taaaacccaa ccatagtatc aacagagtct tttacctgac tcttctcaag
     2521 agcatcctta gcttctttta attttatctc agcattctga acttctgttt ttttagcatc
     2581 tctaatgcta acagcagaat taagagcatt cgtcactgac gaatattcat tcttcttatc
     2641 ctgcaattgt ctctccagtt ttttctgaac atcacgccat ccaggaaatc cggtaacctt
     2701 cacatcaaca cttttttgag cgttttcaag ctcccctgca actctggaaa caatcgcttg
     2761 cttactctgt acatcgcttt cggccttcgc cagttcagct ttagcctcag ccagcttctt
     2821 ctctgccact tccacagccc gatgctcttc atcaagagaa tcctgtcgat ttttctcagc
     2881 ctctgctgcc tccgccattg ctaacaaatg cgcggtttca gcctgttggc gggcaatctc
     2941 atcacgttgg cgttctgctt cccggagcgc tttttctgct gcttcagctt cttcacgggc
     3001 tttttgctct gccttcgcaa ggcgcaaacg ctcagcctct gcctgcattg ccatattatt
     3061 ggcatgagca aggtcagtta ctgatggact acgagcggca ttagcacgta aagcgtcatt
     3121 aacaatatcc ttgagacgtt gagtcagggc atcacgctga gatttcgctt ttgccaatgc
     3181 tgcctcagcc gcagcagcac gggcagcctg ttcagcctga tgttttttca actgcgcggt
     3241 agaccattta gcggttgcat gtatcgcagc agaactttca ctggccccac caaatgctcc
     3301 accgcctcca ccaacatttt cagttccagt tgcaagcgtc gctgtaatcg tatcgccagt
     3361 atcaacccca acagttggga atgacccctc agtagattct gtgctctcca catctggaga
     3421 attatcagtg actttatcca taaagaaatt cctctttgat aattaaaaca ataaattaaa
     3481 aacaacatac tgtacatata accactggtt ttatgtacag taaaaaccta ctactcagca
     3541 ttgtccatgt caagagcatg gattttcatt tttgcaataa ggatcacact atggggaggc
     3601 aggcattgag aacgtcgaaa cagaacaccg gagcaaatca ggatgagata taaaactgtt
     3661 ggatcatgaa aaaacggaga acgatgtgag caaatcaccc cgccataaac tgaacaaaac
     3721 agacaaacga cttctcgaca cccttgttgc tgccggatat gagcatgaca aagcccgtga
     3781 cctcatccag aaacaggttt acacgctgac actggcagat cagcgtcatg tggtcagtga
     3841 aatcagtaat ggtgtgaatc ccacccaggc ttactcggcg gtataccagg caagacgcat
     3901 tcgcctcgcc cgtaaatatc tgaacggtaa aaaggttatg gaagaaaccg gggaaaatac
     3961 gcccccatca gcgtaaggat ttcttttgcc gctccagaga ctccagtttt ttacgcaaat
     4021 cctctctttt ttgggcatct ctggtgccag ccagctctgc tctcaactca tcgatctgaa
     4081 gttgtatctt cagtctgtta ctgaacattt tctgtctggc attaacatcc gcaacaatgc
     4141 cgttttttgt cttctcggcc ttttgttgaa aaacattgct gtccgcatga ctggcaaccg
     4201 aagcagaaag aacactaaaa agcaggactg gcacacattt tttcacggga ttattcctga
     4261 ctcattgacc atcaaatcac attgggagta aaccgacgta tgataagaga tactcttcgg
     4321 atatataact ccctgagtat caagattaaa aacgcaagga gatgtttatg agatctgccg
     4381 ctgccaggct gcttctgata cctctgataa cagcaacaat agctcttaca ggatgcaccc
     4441 caaagaccag cctggaacga catacccggc attatgttta tgcttcagat gatggatttg
     4501 atcctaactt ctacacccag aaagcagaca ccatacgtat gatgctcccg ttctttcagc
     4561 agttccggga tatggggatg aaagacaaag cagccggagt atcagcagaa acggcacagc
     4621 aacgtgtaaa agaattccac tcagaaaaat tttttcactc actccggagc acaacaacct
     4681 ttgctggcag aaaatacaca aacagcgata tgccttcgcc gaaaaaaatg aaactaatgg
     4741 cagacaccat ttctgcggtt tatctcgatg gatacgaggg cagacagtaa gggatttacc
     4801 ataatccctt aattgtacgc accgctgaaa tgcgttcagc gcgatcacgg ctgctgacag
     4861 gtaaaaatgg caacaaacca cccgaaaagc tgccgcgatc gcacctgata aattttaacc
     4921 gtatgcatag ctattcagcc atgtgaataa cgctggtttt gcctgcgtta acctcatgac
     4981 actgtttttt ttccatcttt tcagttgatg acatacgcag acatcgcggg atgaggctga
     5041 ggaatgagcg cgatctggca aagaggcaaa acacagcaac aaaaacgaca ctccagaatc
     5101 gcgcccggat gcgtttttaa cgcgttccgg taccatctgg caacctcccg gaacaactca
     5161 ccgtcacatc cccattgacg ggccacgcca tacccgtgct tcccgttcct gctcttcatg
     5221 ccaggaccgc gcacgctccc gttccaggcg tgcctgcctt tcctgttcat cccttatctg
     5281 ctgttcgtga taaataaccg actcaagcgg tccacctgcc cggctaatct ctgcacctgc
     5341 tgactcaagt cgtcgcactg ttccctcagt tgcccgttct cctgtcgtgt cagctcgaac
     5401 atatgctgca aatccgtgaa ggcgctctcc cagtctttca gccgctgcgt atagtcctgc
     5461 tgcaattgct ctaaggcgtt cagtaagtgc atttccagct ctgtcatact cacttactcc
     5521 ctgaccagtc ttactgcgtt cttcttctcc accgtccagt tgttttcccc cttcaccccg
     5581 gacggcagca ccagaaattt cccgttcctg ccctcgtgat acgtcacacc ccatgttttt
     5641 tcccggagtt tcgccagcgt ctcttcctgg tccctgatag ccaggatgtt cgccgcaatc
     5701 cggctttcct gccactgaat cagcccccat gacgccagaa aaatcccgcc cgtgacgcaa
     5761 agcgccgtca gcgacgggta cagtatccgc cctttgatca gcttccagag cagctcttcc
     5821 tgccgccggg ccagttcgtt ctctgtggcg ctgaactgcg cgttcacggc actgttcagc
     5881 gtctccagtt gttctttcac cgctgctgtg tgtgcgctga tagcgtctct gattttctgc
     5941 ccgtttaagt tcagttccct gtctacagac gcttcgagct tcctgaactc gctgttcagc
     6001 atgttctctg tagagacggc acgctctttc agtttcttct cgaagtctgt ccccatttgt
     6061 aaaagattgc tcatacagcg cccctttcag cctgagatta cgcccgccct ccgggtcggc
     6121 gatactgata ctgctcctgg ttgtcctcac aacctcaaaa cctgccgctg taagcgcctc
     6181 agtgacatcc tgacgcgttt ttagcgctcc ggcatggtaa agagcctcca gtcccctcgt
     6241 aatcgcttct gcggcctcct gtttcgcttt cggcagatta ttcggggtga caagtgtccg
     6301 cctgttctcc ggtgcgttcg ggtcgtgcag cccgtaatgg tgattcacca gtgtctgcca
     6361 ggcattgatt cgcggacggt ccgcccggtc gtaatagggc tggagccgtt ttccgctcgc
     6421 cagctccata ttcgggatga caaaattcag ctcaagacgc cccttgtcct ggtgctccac
     6481 ccacaggatg ctgtactgat ttttttcaag accgggcatc agtacccgct caaagctctc
     6541 catcaccctt tcacgctctc ccggtggcag ggtctgctct gcaaaagaca gaacccccga
     6601 ggtgtatttt ttcgcaaacg gcgtggcatc gatgagttcc cgtacctctt cgggagcacc
     6661 ccgcagaact ctcgcccctt cccggttacg ctcccggccc agcaggtaat caactggacc
     6721 actgccaccg ccttttcccc tggcatgaaa cttaactatc atcccgttct ccctgtttac
     6781 ggacctcatc cctaagctca ctcagttcac gtccgatggc catcagtgca gccaccacat
     6841 gaacccggtc atgccccgac cactgtccgc tgtttatctt ccgggctatc tgattcaggt
     6901 tattgccgac cgaagcgaac tggcgcaaca gcggcggtgc cagtgtcgga agacctgacg
     6961 ttttcgatgg cggtgcaccc aggcagacct tacgcatcca tgacgcaagt tgttttccct
     7021 cacaacgtgc cagcagccgc gcatgttcct catccgtgac ccgtatagtg agcatccttt
     7081 cgcgtttcac cggtatcatt aaaaacctcc gacagactcc ccacacatgg agaaacagaa
     7141 ctgtgactaa acaggaaaaa acagccctta acatggcccg ctttatcaga agccagacgc
     7201 tgacactgct ggaaaaactg aatgaactga acgccgacga ccaggcggac atctgcgaag
     7261 cccttcacga ccacgccgac gagctttacc gcagctgcct cgcacgcttc ggggataacg
     7321 gtgaaaacct ctgacacata cagctcccgg agacggtcac agcttgcctg tgagcggatg
     7381 ccgggagcag acaagcccgt cagggcgcgt cagcaggttt tagcgggtgt cggggcgcag
     7441 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttataccat gcagaatcag
     7501 tgcggattgt atgaaaagtg catcatgccg agtgtgaaat gccgcacaga tgcgtaagga
     7561 gaaaatgcag cttccggtgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg
     7621 ttcgactgcg gcgagcggac tcccctttct caaaggcggt aaccccgtta tccacagaat
     7681 ccggggataa cccgggaaag aacatgtgag caaaaaacga agaccccggg aaaggccgcg
     7741 gcacaggcgc tttttccata ggctccgccc ccctgacgga cttcacaaaa cagacacttt
     7801 ttcggtgcat ctgtgggagc cttacggctc aaccatgaaa tccgccagcg gtggcgaaac
     7861 ccgacgtggc ttaaagatca tcaccgtttc cggtgaatcg ctccctcctg cgcccccacg
     7921 ttcccgccat atcgattacc ggataccttt tccgcctttc tcccttgcgg gacacgtggc
     7981 gctttctcat agctcacgtc ttctggtatc tcggggcgac ttaggcagtt cgctccgggc
     8041 cgggctgtat gcaggaaccc cccgttcagc ccgactgctg cgccttctcc ggtaactgtt
     8101 ctcttgggtc caacccggaa aggacacgac ttaacgccag tggcagcagc cattggtagc
     8161 caggttagca gagcgagatt tataagttag taacagagtt cttgaagtat cagcctgatt
     8221 ttgactacac tagaaggaca agttttgggt atctgcgctc tgctgaagcc agttaccttc
     8281 ggaaaaagag ttggtagatc ttgatccggc aaacaaacca ccgttggtag cggtggtttt
     8341 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8401 ttttctactg aaccgcgatc cctgtcagtt cagaagagga agatggagcg accgtccctc
     8461 cttgtacatc actaatataa tttgcttgac atcccgacaa ggataatatt gtaattatca
     8521 aaaacgttat tttcattcta cccctataac aacataaata atgaccgtaa aatactacct
     8581 acacaattta ctagagtcac ttatcccatg gctattctat ttgttattaa actataaga
//
