LOCUS       NZ_CP021101             8618 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Escherichia coli strain 13P477T plasmid p13P477T-9, complete
            sequence.
ACCESSION   NZ_CP021101
VERSION     NZ_CP021101.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06219553
            Assembly: GCF_002951655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Ho,P.L., Lau,A., Liu,C.J., Lai,E.L.Y., Chow,K.H., Tse,C.W.S. and
            Que,T.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Microbiology, The University of Hong Kong,
            102 Pok Fu Lam Road, Hong Kong, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. Nov-2016
            Genome Coverage                   :: 200
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 04:11:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,487
            CDSs (total)                      :: 5,362
            Genes (coding)                    :: 5,063
            CDSs (with protein)               :: 5,063
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 299
            CDSs (without protein)            :: 299
            Pseudo Genes (ambiguous residues) :: 0 of 299
            Pseudo Genes (frameshifted)       :: 156 of 299
            Pseudo Genes (incomplete)         :: 162 of 299
            Pseudo Genes (internal stop)      :: 54 of 299
            Pseudo Genes (multiple problems)  :: 69 of 299
            Pseudo Genes (short protein)      :: 4 of 299
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP021101.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="13P477T"
                     /isolation_source="stool"
                     /host="pig"
                     /db_xref="taxon:562"
                     /plasmid="p13P477T-9"
                     /geo_loc_name="Hong Kong"
                     /collection_date="2013-04"
     gene            complement(join(<8618..<8618,1..446))
                     /locus_tag="BWI89_RS29775"
                     /pseudo=""
     CDS             complement(join(<8618..<8618,1..446))
                     /locus_tag="BWI89_RS29775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /name="colicin-10"
     gene            705..1142
                     /locus_tag="BWI89_RS28335"
                     /old_locus_tag="BWI89_28320"
     CDS             705..1142
                     /locus_tag="BWI89_RS28335"
                     /old_locus_tag="BWI89_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024189453.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTSQDGRR
                     EFETSELIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLQNEIAQLRQALEQEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            1223..1642
                     /locus_tag="BWI89_RS28340"
                     /old_locus_tag="BWI89_28325"
     CDS             1223..1642
                     /locus_tag="BWI89_RS28340"
                     /old_locus_tag="BWI89_28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_016247941.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVELHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2203..2436)
                     /locus_tag="BWI89_RS29500"
     CDS             complement(2203..2436)
                     /locus_tag="BWI89_RS29500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2443..2973)
                     /locus_tag="BWI89_RS29505"
     CDS             complement(2443..2973)
                     /locus_tag="BWI89_RS29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_143361689.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNALRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3011..3661)
                     /locus_tag="BWI89_RS30345"
                     /pseudo=""
     CDS             complement(<3011..3661)
                     /locus_tag="BWI89_RS30345"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3651..3974)
                     /locus_tag="BWI89_RS28360"
                     /old_locus_tag="BWI89_28345"
     CDS             complement(3651..3974)
                     /locus_tag="BWI89_RS28360"
                     /old_locus_tag="BWI89_28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_106505284.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVVALMAIERELRSLRLVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4039..4230
                     /locus_tag="BWI89_RS28365"
                     /old_locus_tag="BWI89_28350"
     CDS             4039..4230
                     /locus_tag="BWI89_RS28365"
                     /old_locus_tag="BWI89_28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5254..5391)
                     /locus_tag="BWI89_RS28385"
                     /old_locus_tag="BWI89_28370"
     CDS             complement(5254..5391)
                     /locus_tag="BWI89_RS28385"
                     /old_locus_tag="BWI89_28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001329514.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            5553..5780
                     /locus_tag="BWI89_RS28390"
                     /old_locus_tag="BWI89_28375"
     CDS             5553..5780
                     /locus_tag="BWI89_RS28390"
                     /old_locus_tag="BWI89_28375"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_015056439.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(5777..7344)
                     /locus_tag="BWI89_RS30350"
                     /pseudo=""
     CDS             complement(5777..7344)
                     /locus_tag="BWI89_RS30350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /name="colicin-like pore-forming protein"
     gene            7604..8041
                     /locus_tag="BWI89_RS28405"
                     /old_locus_tag="BWI89_28390"
     CDS             7604..8041
                     /locus_tag="BWI89_RS28405"
                     /old_locus_tag="BWI89_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024189453.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTSQDGRR
                     EFETSELIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLQNEIAQLRQALEQEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            8122..8541
                     /locus_tag="BWI89_RS28410"
                     /old_locus_tag="BWI89_28395"
     CDS             8122..8541
                     /locus_tag="BWI89_RS28410"
                     /old_locus_tag="BWI89_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_016247941.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVELHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     Typing          complement(4317..4403)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="100.0"
                     /evalue="1.13e-36"
     Typing          4414..4971
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2100..3661)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.697"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3658..4007)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.162"
                     /coverage="99.0"
                     /evalue="1.09e-64"
ORIGIN
        1 aaagcctttt ccgcagcttc cgcttcttta cgagcttttt cttctgcttt tgcaagcgca
       61 accgctctgc ttctgcctgc atcgctgcat tattggcatg agccaggtca ataacctccg
      121 gatgagtgga attatggcga agcgcctcat tcacaatatc cttcagatgt tgagtcagcg
      181 catcccggtt tgcttttgct ttagcctgtg cttctgcggc agctttttgc tcgggcagcc
      241 tgttctgcct gcgttttctt caattgagca gtagaccatt tagctgtggc atgaatggct
      301 gcagaacttt cacttttgct acctccagtt ccaccaccac cgccagagcc actcccgtct
      361 ggattaccat tcaaaagagt aatgattacc tgccccttat catcataagg aacaccatct
      421 ttatagtacg ctacagctgt ttccattata aaatcctctt tgacattaaa aacaaatagt
      481 taaaagcaat atactgtata tataaccact ggttttatat acagcttaaa attacgccgc
      541 tgcattttcc ctgtcaaccc tgtggatttt catttttgtg aaaacgatca aaaaacagcc
      601 acccacacca cgtgctattt ccacccgatg ccacaaaaac cagcacaaac attaccgttc
      661 tccggcctca gtatgtttta ctgaaaccat gatatgagac accaatggga cactgtcact
      721 tcatggcatg gcacacactc caggacgcac tgaaaatgac aggcagatcg cgttcacagt
      781 tttaccgtga tatgcgcgca ggccttgtca gttaccgtac cagccaggac ggacgacggg
      841 agtttgaaac cagtgaactg atccgggcat acggcgaatt aaagcaggat gagacaccag
      901 aaaggcacag tgcgggacag gcagaaaatc cacatgatca gcagacagaa cgcattctcc
      961 gggagctgaa tgagctgaaa caatgcctga cgctgatgct tgaggataaa caagcccagg
     1021 atacggagcg ccggcgccag gaagcagaac gggaacagct acagaatgag atagcccagc
     1081 tcaggcaggc gctggagcag gaaaagaaac ggggattctg gtcccggttg tttggtcgct
     1141 gaacgctgtc atggactgat gataaaatag tcttctgata ataactcacc gaaaataaat
     1201 actttaaggt agggagactc tcatgagacg taccggaaac aaactttgtc ttatcgccat
     1261 gataacagca acagtagctc tcacagcctg taccccaaag ggcagcgtgg aactacatac
     1321 ccggcattac gtatatgctt ctgatgacgg ttttgatccc aacttttcca cccaaaaagc
     1381 cgacacaaca cgaatgatgg tgcctttttt ccggcagttc tgggatatgg gagcaaagga
     1441 taaagcgaca ggaaaatcac ggagtgatgt gcagcaacgc attcagcagt ttcacagcca
     1501 ggaattttta aactcactcc ggggcacaac tcaatttgcg ggtactgatt accgcagcaa
     1561 agaccttacc ccgaaaaaat ccaggctgct ggctgagacg atttctgcgg tttatctcga
     1621 tggctatgag ggaagacagt aagtggattt accataatcc cttaattgta cgcaccgctg
     1681 aaacgcgttc ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa
     1741 aactgccgcg atcgcacccg gtaaatttta accacatgca tagctatgca gccatgtgaa
     1801 tcacgcagga attgccagcc agagacagct gaaacggatt tttttcatgc tttcggaagc
     1861 gaagaagacg gggaccggag cgggaaaaca gaagttcagg ggaatgagcg cgatctggca
     1921 atagaggcaa aacacagcaa caaaagacac accagaatcg cgcccgtatg cgttttaacg
     1981 cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc cgaaccaacc
     2041 attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc tctgattacc
     2101 atcccagcga agggccatcc agtgtccgtt cctgtatttc cagctggcgc tcacgctcca
     2161 gggataactc atgttcgcgt tcctgtatca gccgttcgtc ccttatctcc tgttctcgct
     2221 gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg
     2281 catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa
     2341 ttccacgaag gcgctctccc attcgttcag ccgctgcata tagtcctgtt gcagttgctc
     2401 taatgcgttc agcaaatgtc tttccagttc cgtcatgctc tgttactcct gtaccagcct
     2461 taaggcattc tgtttcccgt tttccatcgt ccagttcgtt tcggctttca tcccctcggg
     2521 caacaccaga aaacgtccgt tgctcccctc gtgataacgc acgccccatg tcttcgcgtt
     2581 cagcttctcc agactctcgt tctgcttcct gatttcttcc aggttcgcct gtatcatccc
     2641 gccaagatac cagagcgtcc cgccactcac ggtaaacagg aaaatgacca tccccagtaa
     2701 catcatgccc gtattccctg ccagctttaa cacgtccttc ctgtgctgca tcatcgcctc
     2761 tttcacccct tcccggtgtt ttttcagtga ttcctctgtc gaagctgtga acagggctat
     2821 agcatctttg attttcgtct cgtttgatgt cacagccttg cttacagatt cttcgagctt
     2881 cctgaactcg tcgttcagca ttttctctgt agattcggct ctctctttca gctttttctc
     2941 gaactccgcg cccgtctgta aaagattgct catagaatgc tcctttcagt ctgatattcc
     3001 tcccgccgtt cgggtctgca atgctgatac tgcttcgcgt caccctgacc acttcaagtc
     3061 ccgcctccgt gagcgcctga atcacatcct gacgaccttt tatctctccg gcatggtaaa
     3121 gcgcgtctat cccgcgtgta acgccctcag caatcgcctg tttcgtttcc ggcagattat
     3181 cagggagtgt cagcgtcctg cggttctccg gggcgttcgg gtcatgcagc ccgtaatggt
     3241 gattaaccag cgtctgccag gcatcaattc taggcctgtc tgcccggtcg tagtacggct
     3301 ggaggcgttt tccgctctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc
     3361 ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca
     3421 gtacacgctc aaagctcgcc atcacctttt cacgtcctcc cggcggcagc tccttctcag
     3481 cgaacgacag aacacctgac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc
     3541 ggacttcttc cggattaccc tgaagcaccg ttgcaccttc ccggttacgc tcccttccca
     3601 acaggtaatc aaccggacca ctgccaccgc cttttcccct ggcatgaaat ttaacaatca
     3661 tcccgcgctc cctgctccct gacgaccagc cgtaagctgc gcaactctct ctcgatggcc
     3721 atcagtgcga ccaccacctg aacccggtca ccggaagacc actgcccgct gtttaccttc
     3781 ctcgctgtct gattcaggtt attcccgatg gcggccagct gacgcagtaa cggcggtgcc
     3841 agtgtcggca gcttcccgga acgggcaacc ggctcaccca gacagacccg ccgcatccat
     3901 accgccagct gtttaccctc acagcgttcc agtaaccgtg catgttcatc atcagtaacc
     3961 cgtatcgtga gcatcctctc gcgtttcatc ggtatcatta cccccatgaa cagaaatccc
     4021 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     4081 atcagaagcc agacattaac gctgctggag aagctcaacg aactggacgc ggatgaacag
     4141 gcagacatct gtgaatctct tcacgaccac gccgatgagc tttaccgcag ttgcctcgct
     4201 cgcttcgggg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     4261 tgtctgtgag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtttttgc
     4321 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     4381 accatgcggc atcagtgcgg attgtatgaa aagtgcgcca tgccgggtgt gaaatgccgc
     4441 acagatgtgt aaggagaaaa tgcaacttcc gacgctcttc cgcttcctcg ctcactgact
     4501 cgctacgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag acggtaatgc
     4561 ggttatccac agaatcaggg gataacacca aaagaaacat gtgagcaaaa aacaagaacc
     4621 cagagaaggc cacgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4681 aaaaccgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4741 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4801 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgttggtatc
     4861 tcggctcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4921 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4981 tagcgccact ggcagcagcc gttggtaact tggttagcag agcgatgatg gcgcaaatgg
     5041 tgcaacagag ttcttgaagt agtggcccga ctacggctac actggaagga cagtatttgg
     5101 tatctgcgct ctgctgaagc cagttacctc ggttaagcag ttccccaact gacttaacct
     5161 tcgatcaaac cgcctcccca ggcggttttt tcgtttacag gcagcagatt acgcgcagaa
     5221 aaaaaggatc tcaagaagat cctttgatct tttctactga acagagatcc ccgtcaattt
     5281 agaagaagat gatggagcca cagtccctcc ctgaacatca cgtatatagt tagcctgaca
     5341 tccaacaagg aggtttatag cgaatattcc cacaaaaaat cttttcctca taactcgatc
     5401 cttataaaat gaaaagaata tataacgagg tttaatttat gagtttaaga tactacataa
     5461 aaaatatttt gtttggccta tactgcgcac ttatatatat atatcttata acaaaaaaca
     5521 acgaagggta ttatttccta gcgtcagata agatgctata cgcaatagtg ataagcacta
     5581 ttttatgccc atattcaaaa tatgctattg aacacatatt ttttaagttc ataaagaaag
     5641 attttttcag aaaaagaaaa aacctaaata atgcccccgt ggcaaaatta aacctattta
     5701 tgctatacaa tctactttgt ttggtcctag caatcccatt tggattgcta ggactgttta
     5761 tatcaataaa gaataattaa atacccaaca cctcatttat agtattaagt ttattcttat
     5821 caataaaagc acatagaatg cctgtaataa tagcaatccc ccagatacct aatgtagttc
     5881 cagcaagcat actaaaaagt aaaacaacaa cataactaac tccagcatct acagctttct
     5941 tctctaatgt aagaaatagt ggcttccagt cacctgtatc cttaattttt aggatatcag
     6001 ataccacatc atatccaaaa gaaacatgtc ccgtaatctt caagtactta gcaaactgat
     6061 ctaaatgctt agcccagtct tcatacttaa ccgactccag tgcattgaaa attgcatcac
     6121 ggtctgcttt gctgaatttc ttatttaaaa acatccttgt atttttcaaa agcagctaga
     6181 gcttcattca cattgctgat tttcttacct ttagatttat cagcaagttc ctgtgccatt
     6241 tttgaatatt tttcaccata tttttcagat agcgtttgat aaaagctaac tgttgcatcc
     6301 acagcatcct taatctgcga gttcaggaga ttagtctgtg ctattttcaa attatcttca
     6361 gcatcatgaa tacgtgaaac tgctgtactt cgcttattat tagcctgaga aatagccttt
     6421 tggattccat ttatctcaga gctaatctgg ttaagacgcg tttctgatgc tgttacctgc
     6481 ttttgttttt cctccatctc atcaccagct ctcgcccgta gtctggtggc ctcaaaaaaa
     6541 ggacgactct gaagcggatc attcgcccta ggagataaca gtttaaccct ttcatccagt
     6601 tctttatatt tagcagatgc ctgatccaac tcatttcgtt ttccggacag cgtattcgtt
     6661 tctgcatcac gggcatgaat accggagctt aaacgggtat tgagcgtatt aatctcttca
     6721 tccactttcg ccagctcaga ttgtgcagca gcaagatttt tttgtgccac ctccacagcc
     6781 cgggcctctt cactcaatgc tgccaggcgt ttctcttcat cctcagccag tttcaattgg
     6841 cgttctgttt cagcctgctc cttctctatc tctttacgtc gttgttctgc ttcctgaaaa
     6901 gccttttccg cagcttccgc ttctttacga gctttttctt ctgcttttgc aaggcgcaac
     6961 cgctctgctt ctgcctgcat cgctgcatta ttggcatgag ccaggtcaat aacctccgga
     7021 tgagtggaat tatggcgaag cgcctcattc acaatatcct tcagatgttg agtcagcgca
     7081 tcccggtttg ctttttgctt tagcctgtgc ttctgcggca gcttttgctc gggcagcctg
     7141 ttctgcctgc gttttcttca attgagcagt agaccattta gctgtggcat gaatggctgc
     7201 agaactttca cttttgctac ctccagttcc accaccaccg ccagagccac tcccgtctgg
     7261 attaccattc aaaagagtaa tgattacctg ccccttatca tcataaggaa caccatcttt
     7321 atagtacgct acagctgttt ccattataaa atcctctttg acattaaaaa acaaatagtt
     7381 aaaagcaata tactgtatat ataaccactg gttttatata cagcttaaaa ttacgccgct
     7441 gcattttccc tgtcaaccct gtggattttc atttttgtga aaacgatcaa aaaacagcca
     7501 cccacaccac gtgctatttc cacccgatgc cacaaaaacc agcacaaaca ttaccgttct
     7561 ccggcctcag tatgttttac tgaaaccatg atatgagaca ccaatgggac actgtcactt
     7621 catggcatgg cacacactcc aggacgcact gaaaatgaca ggcagatcgc gttcacagtt
     7681 ttaccgtgat atgcgcgcag gccttgtcag ttaccgtacc agccaggacg gacgacggga
     7741 gtttgaaacc agtgaactga tccgggcata cggcgaatta aagcaggatg agacaccaga
     7801 aaggcacagt gcgggacagg cagaaaatcc acatgatcag cagacagaac gcattctccg
     7861 ggagctgaat gagctgaaac aatgcctgac gctgatgctt gaggataaac aagcccagga
     7921 tacggagcgc cggcgccagg aagcagaacg ggaacagcta cagaatgaga tagcccagct
     7981 caggcaggcg ctggagcagg aaaagaaacg gggattctgg tcccggttgt ttggtcgctg
     8041 aacgctgtca tggactgatg ataaaatagt cttctgataa taactcaccg aaaataaata
     8101 ctttaaggta gggagactct catgagacgt accggaaaca aactttgtct tatcgccatg
     8161 ataacagcaa cagtagctct cacagcctgt accccaaagg gcagcgtgga actacatacc
     8221 cggcattacg tatatgcttc tgatgacggt tttgatccca acttttccac ccaaaaagcc
     8281 gacacaacac gaatgatggt gccttttttc cggcagttct gggatatggg agcaaaggat
     8341 aaagcgacag gaaaatcacg gagtgatgtg cagcaacgca ttcagcagtt tcacagccag
     8401 gaatttttaa actcactccg gggcacaact caatttgcgg gtactgatta ccgcagcaaa
     8461 gaccttaccc cgaaaaaatc caggctgctg gctgagacga tttctgcggt ttatctcgat
     8521 ggctatgagg gaagacagta agtggattta ccataatccc ttaattgtac gcaccgctga
     8581 aacgcgttcg gcgcgatcac ggcagcagac aggtaaaa
//
